RESUMO
Monoclonal antibodies were produced against Taylorella equigenitalis using two reference strains. Out of the 79 hybridoma clones shown to express antibodies to T equigenitalis by indirect immunofluorescence assay, 16 were selected for monoclonal antibody production and characterization. These clones recognized different field strains of T equigenitalis isolated in France. They showed no cross-reaction with bacterial strains with previously reported antigenic cross-reactivity, nor did they react with other bacteria commonly found in genital flora. The epitopes recognized by eight of the monoclonal antibodies were situated in proteins of 150, 120, 52.7 and 22 kDa. These epitopes were resistant to the extraction denaturing conditions. These monoclonal antibodies could be used as reagents for specific detection of T equigenitalis.
Assuntos
Anticorpos Antibacterianos/biossíntese , Anticorpos Monoclonais/biossíntese , Especificidade de Anticorpos , Haemophilus/imunologia , Animais , Anticorpos Antibacterianos/imunologia , Anticorpos Monoclonais/imunologia , Antígenos de Bactérias/imunologia , Reações Cruzadas , Eletroforese em Gel de Poliacrilamida/veterinária , Endometrite/diagnóstico , Endometrite/veterinária , Epitopos/imunologia , Feminino , Técnica Indireta de Fluorescência para Anticorpo/veterinária , Infecções por Haemophilus/diagnóstico , Infecções por Haemophilus/veterinária , Doenças dos Cavalos/diagnóstico , Cavalos , Hibridomas , Immunoblotting/veterinária , Camundongos , Camundongos Endogâmicos BALB CRESUMO
The Wyoming strain of equine infectious anemia virus was adapted to cell cultures by 7 passages in horse leukocytes and 14 passages in fetal equine dermal and kidney cells. The virus was made evident by electron microscopy and immunodiffusion tests with antigens prepared from culture fluids.