Clinical results of critical rehabilitation pathway after total knee arthroplasty / 中国骨伤

OBJECTIVE@#To investigate the clinical results of the application of critical rehabilitation pathway in the rehabilitation after total knee arthroplasty.@*METHODS@#From March 2015 to December 2019, 67 patients with total knee arthroplasty (TKA) were included. There were 49 females and 18 males, 42 cases on the left and 25 cases on the right, with an average age of 60 to 81(70.72±5.92) years old. Critical rehabilitation paths included intensive strength and gait rehabilitation exercises. All patients were evaluated before operation and 3, 12 months after operation. The evaluation indexes included stair climbing test (SCT), six minute walk test (6MWT), quadriceps and hamstring strength, range of motion, visual pain scale (VAS), Western Ontario McMasterUniversity Osteoarthritis score(WOMAC).@*RESULTS@#All the patients completed the entire pathway and the assessment. The results of pre-operative, 3 months after surgery and 12 months after surgery were as follows respectively. SCT-up: (16.32±3.58) s, (18.16±2.46) s, (11.00±1.29) s, @*CONCLUSION@#Critical rehabilitation path is safe and effective. The knee function of patients who receive critical rehabilitation path after TKA is significantly improved in the first 12 months after operation.

DNA barcoding identification of original plants of a rare medicinal material Resina Draconis and related Dracaena species / 中国中药杂志

Resina Draconis, a rare and precious traditional medicine in China, is known as the "holy medicine for promoting blood circulation". According to the national drug standard, it's derived from the resin extracted from the wood of Dracaena cochinchinensis, a Liliaceae plant. In addition, a variety of Dracaena species all over the world can form red resins, and there is currently no molecular identification method that can efficiently identify the origin of Dracaena medicinal materials. In this study, seven species of Dracaena distributed in China were selected as the research objects. Four commonly used DNA barcodes(ITS2, matK, rbcL and psbA-trnH), and four highly variable regions(trnP-psaJ, psbK-psbI, trnT-trnL, clpP) in chloroplast genome were used to evaluate the identification efficiency of Dracaena species. The results showed that clpP sequence fragment could accurately identify seven species of Dracaena plants. However, due to the long sequence of clpP fragment, there were potential problems in the practical application process. We found that the combined fragment "psbK-psbI+ trnP-psaJ" can also be used for accurate molecular identification of the Resina Draconis origin plants and relative species of Dracaena, which were both relatively short sequences in the combined fragment, showing high success rates of amplification and sequencing. Therefore, the "psbK-psbI+ trnP-psaJ" combined fragment can be used as the DNA barcode fragments for molecular identification of Resina Dracon's origin plants and relative species of Dracaena. Research on the identification of Dracaena species, the results of this study can be used to accurately identify the original material of Resina Draconis, and providing effective means for identification, rational development and application of Resina Draconis base source.

Effect of atorvastatin on expressions of intercellular adhesion molecule-1 and transforming growth factor-β1 in the spinal cord of rats with experimental autoimmune encephalomyelitis / 中华神经医学杂志

Objective To study the effect of atorvastatin on the expressions of intercellular adhesion molecule-1 (ICAM-1) and transforming growth factor-β1 (TGF-β1) in the spinal cord of rats with experimental autoimmune encephalomyelitis (EAE).Methods Eighty healthy female Wistar rats were randomly divided into 4 groups:normal control group (n=20),EAE model group (n=20),low-dose treatment group (n=20) and high-dose treatment group (n=20).Each group was equally divided into 2 sub-groups:14-d group and 21-d group according to the day that they were sacrificed.EAE rat models were established by immunizing the rats with fresh guinea-pig spinal cord homogenate plus complete Freund's Adjuvat (CFA).Rats of the treatment groups were fed with atorvastatin at dasage of 2 mg/(kg·d)and 10 mg/ (kg·d),respectively; and rats of the normal control group and EAE model group were fed with physiological saline.The severity of EAE was scored according to the signs and symptoms,and NIHSS was performed.Pathological changes were observed with the aid of HE staining.The levels of ICAM-1 and TGF-β1 were detected by immunohistochemistry.Results The rats in the high-dose treatment group had significantly lower incidence of disease,decreased CNS inflammation focus,lower level of ICAM-1 and higher level of TGF-β1 as compared with rats of the EAE model group and low-dose treatment group (P＜0.05).Conclusion Atorvastatin could ameliorate EAE of rats in a dose-dependent manner,whose effect might be related to the decreased expression of ICAM-1 and increased expression of TGF-β1.

Histological study about the effect of orthodontic loading time and duration on the periodontal repair in autologous tooth transplantation / 华西口腔医学杂志

<p><b>OBJECTIVE</b>The study established an animal model of autologous tooth transplantation in dogs and aimed to explore the effects of orthodontic loading time and duration on the periodontal repair in autologous tooth transplantation.</p><p><b>METHODS</b>12 Beagle dogs were selected and grouped randomly in this research. Four first premolar teeth in every dog were carried on autologous transplant operation. These teeth were exerted the orthodontic force after the operation in 2, 4, 8 and 12 weeks respectively. The loading duration was 1, 2 and 4 weeks respectively. We examined the dental and periodontal tissue samples in first premolar teeth by histological study, measured root absorption rate and analyzed reconstruction condition of periodontal tissue with statistical methods.</p><p><b>RESULTS</b>Root absorption rates of the experimental group which was exerted the orthodontic force were lower than those of the control group. The difference between two groups had statistical significance (P < 0.05). Root absorption rates in different levels of orthodontic loading time (X) and the duration (Y) had no statistical significance (P > 0.05), but these two factors had interaction effect (P(X x Y) < 0.05).</p><p><b>CONCLUSION</b>Orthodontic force contributes to the improvement of root absorption and reconstruction of periodontal tissue in transplant teeth. The orthodontic loading time and the duration have no obvious effect on root absorption rate of transplant teeth. When X is two weeks and Y is four weeks, it is most beneficial for the reconstruction effect of periodontal tissues in transplant teeth.</p>

Community based rehabilitation of cognitive deficits in stroke patients / 中华物理医学与康复杂志

Objective To investigate the effects of cognitive interventions (CIs) in the context of communi- ty based rehabilitation (CBR) on cognitive deficits (CDs) in stroke patients.Methods Ninety-two stoke patients with CDs were randomly divided into a CI group and a control group.All patients were treated with conventional CBR.In addition,the patients in the CI group were also treated with special intervention therapy.The patients in both groups were assessed with the neurological and cognitive status examination (NCSE) for cognitive functioning, the FCA for motor function and the BI for their ability in the activities of daily living.Results The NCSE,FCA and BI scores in the cognitive intervention group after treatment were significantly higher than those before treatment and also significantly higher than those in the control group after treatment.Conclusion CIs can not only improve CDs,but also enhance recovery of motor function and ADL.