Diagnostics possibilities of erythrocytes analysis by the method of laser interference microscopy.

The morphofunctional state of erythrocytes significantly changes by the onset and progression of various diseases, as well as in critical conditions, injuries, accompanied by severe tissue damage. This leads to a violation of microcirculation and has an effect on hemorheology in general. In connection with this, there is a need for the development of adequate methods for assessing the functional activity of erythrocytes. The aim of the work was to study the possibilities of laser interference microscopy in the analysis of the functional state of erythrocytes. The results allowed us to quantify the state of the phase height and phase diameter of erythrocytes. Analysis of the surface nanostructure gave a qualitative assessment of the functional activity of cells. The following patterns were revealed: the action of adrenaline causes the appearance of echinocytes with an increase in phase height and decrease phase diameter and the appearance of spicules on the surface of cells. The action of cortisol leads to a decrease in the phase height and the phase diameter with the appearance of numerous loosening of the structure of the cell surface. Moreover, the traditional method of analyzing the distribution of erythrocytes in a population by morphology (discocytes, stomatocytes, and degeneratively altered cell forms) did not reveal statistically significant differences. Thus, the results indicate that the interference microscopy method allows a qualitative assessment of the morphofunctional activity of native cells. This increases the information content of the analysis and leads to the objectification of data on the functional capabilities of erythrocytes.

[The evaluation of genotoxic effects in buccal epithelium under disorders of adaption status of organism.]

The cyto-morphologic analysis of buccal epithelium is one of technique of evaluation of adaptation status of organism and xenogeneic intoxication. The analysis of reactivity of cells of buccal epithelium is used to obtain information concerning genetic alterations in human cells that is extremely important for proper decision making related to issues of population health protection. The smoking results in manifestation of tension of adaptation mechanisms and significant increasing of risk of development cytogenetic disorders in cells of buccal epithelium of oral cavity. The study was carried out concerning morphology of buccal epithelial cells of smoking youths. The cytogram demonstrated a reliable decreasing of number of normal cells and increasing of percentage of cells with various cytogenetic alterations. The cells with micro-nuclei are observed twice more often that can be considered as an integral indicator of genetic disorders in inter phase. In smokers, among cells with signs of completion of nucleus destruction the cells with karyorrhexis are registered reliably more often. The results of micro-nucleus test are an unfavorable prognostic indication testifying high degree of genotoxicity of tobacco smoke and meaning a disorder of cytogenetic homeostasis and decreasing of adaptation reserve of organism.

[The evaluation of adaptive possibilities of organism under therapy of diabetes mellitus type I and II.]

The article presents results of the study of electrophoretic agility of erythrocytes, their pro-oxidant characteristics and intra-cellular concentration of adenosine triphosphate (ATP) and 2,3-diphosphoglycerate (2,3-DPG) in patients with diabetes mellitus type I and II under implementation of therapy. The sampling included 46 patients with diabetes mellitus type I and II. The control group consisted of 22 healthy volunteers. 20 patients with diabetes mellitus type i received insulin protaphan and insulin actrapid, 26 patients with diabetes mellitus type II received preparations of sulfanylurea, biguanides, incretins. The analysis of blood was applied before and after course of treatment. The electrophoretic agility of erythrocytes was analyzed, including concentration of malonic dialdehyde in them, content of 2,3-DPG and ATP. In case of diabetes mellitus type I and II, the study established increasing of content of malonic dialdehyde, decreasing of electrophoretic agility of erythrocytes, concentration of ATP and 2,3-DPG in erythrocytes concerning physiological standard, mostly expressed in patients with diabetes mellitus type I. The therapy decreased concentration of malonic dialdehyde and increased electrophoretic agility of erythrocytes, content of ATP and 2,3-DPG as compared with indices before treatment. In patients with diabetes mellitus type II during treatment reduction of electrophoretic agility of erythrocytes and 2,3-DPG up to physiological standard was observed. The positive effect of the given clinical strategies during treatment of diabetes mellitus type I and II is conditioned by decreasing of stress reaction and activation of adaptation processes that is manifested at the level of micro-circulation by amelioration of oxygen-transport function of blood at the expense of increasing of electrophoretic agility of erythrocytes, level of 2,3-DPG and decreasing of destructive processes of membranes of erythrocytes.

[Continuous ambulatory peritoneal dialysis in patients with diabetes mellitus]. / Postoiannyi ambulatornyi peritoneal'nyi dializ u bol'nykh sakharnym diabetom.

AIM: To analyse the results of continuous ambulatory peritoneal dialysis (CAPD) in diabetes mellitus (DM) patients, to specify complications, to evaluate transport characteristics of peritoneal membrane. MATERIAL AND METHODS: The ability of peritoneal membrane to eliminate low-molecular substances (creatinine, urea), electrolytes and to evaluate treatment efficacy by residual renal function (peritoneal equilibration, total urine and creatinine clearance). RESULTS: DM patients on CAPD developed vascular complications typical for DM:gangrene (n = 2), diabetic foot (n = 4), acute disorder of cerebral circulation (n = 3), acute myocardial infarction (n = 3). Diabetics' residual renal function deteriorated faster than in patients with non-diabetic uremia, though transport characteristics of the peritoneum in diabetics and non-diabetics were the same. Peritonitis in diabetics was observed much less frequently than in non-diabetics. CONCLUSION: CAPD is an adequate replacement therapy in patients with uremia of different origin including diabetes. In the course of the treatment DM patients develop complications typical for basic disease but their frequency is unrelated to CAPD.

[Low doses of recombinant erythropoietin in the treatment of renal anemia in patients on chronic hemodialysis and ambulatory peritoneal dialysis]. / Nizkie dozy rekombinantnogo éritropoétina v lechenii pochechnoi anemii u bol'nykh, nakhodiashchikhsia na khronicheskom gemodialize i na ambulatornom peritoneal'nom dialize.

AIM: A comparative study of efficiency and safety of low-dose erythropoietin (EP) in two groups of patients with chronic renal failure (CRF): patients on chronic hemodialysis (CHD) and patients on continuous ambulatory hemodialysis (CAHD). MATERIALS AND METHODS: 51 CRF adult patients with renal anemia on hemodialysis entered the trial: 34 CHD and 17 CAHD patients. EP compounds were injected s.c. in a dose 1000-2000 U 2-3 times a week. RESULTS: EP treatment provided a rapid correction of renal anemia in the majority of patients. After 3-month EP therapy a mean increment of Hct (Hct delta) was much greater (p < 0.05) in CAHD than CHD patients (12.2 +/- 6.0 and 9.0 +/- 5.1%, respectively), though EP dose were the same in both the groups. CONCLUSION: Low doses of recombinant human EP injected subcutaneously were effective and safe for correction of anemia in both CHD and CAHD. In CAHD patients EP effectiveness was much higher than in CHD patients.

Induction of IL-6 and IL-10 production by recombinant HIV-1 envelope glycoprotein 41 (gp41) in the THP-1 human monocytic cell line.

Elevated levels of circulating monokines (IL-6, IL-1, and TNF alpha) have been seen in HIV-1 infection, and the overproduction of these cytokines could contribute to AIDS pathogenesis in various ways. In previous work, we had seen that exposure of human monocytes to HIV-1, including inactivated, noninfectious HIV, led to rapid IL-6 gene expression and secretion. To investigate cytokine production in response to components of HIV by monocytes/macrophages, production of IL-6 and IL-10 were examined in a human monocytic cell line, THP-1, stimulated by HIV proteins. IL-6 production was induced in THP-1 cells by a detergent lysate of HIV, particularly fractions at molecular weight of 25-50 kDa. Recombinant HIV envelope glycoprotein 41 (gp41), but not gp120 or p24, also was seen to induce significant IL-6 production by THP-1 cells. These results suggest that gp41, transmembrane protein, is the primary HIV-encoded protein involved in inducing IL-6 production. IL-10 was also produced with delayed kinetics, following IL-6 production in THP-1 cells stimulated by gp41. To investigate a possible regulatory role for IL-10 in HIV-induced monokine production, recombinant IL-10 was added to gp41-exposed THP-1 cells. IL-10 inhibited gp41-induced IL-6 production and reduced the expression of IL-6 mRNA. When anti-human IL-10 neutralizing antibody was added to THP-1 cells, IL-6 production was enhanced. These results suggest that the IL-6 production may be downregulated by endogenously produced IL-10 and that IL-10 may downregulate cytokine production by HIV-activated monocytes via an autoregulatory mechanism.

[A method for performing stimulation electromyography of the masticatory muscles in man]. / Metodika provedeniia stimuliatsionnoi élektromiografii zhevatel'nykh myshts u cheloveka.

A principal block scheme of an apparatus complex is suggested and a method for examination of masticatory muscle function is described. The method is based on electrostimulation of masticatory nerve followed by registration and analysis of the masticatory muscle action evoked potentials. The block scheme of the apparatus complex includes a universal electrostimulator ESU-2. Examinations of 30 healthy volunteers and 30 patients with unilateral fractures of the mandible helped define the major parameters of stimulation electromyography. The patients were treated by splints applied on the teeth with fixation on both jaws. By day 28 of the investigation the M-response amplitude dropped more than twofold and its length by 1.5 times as against the mean parameters in health.

[Stimulation of the immune response to immobilized antigen in an in vitro system with B-activin]. / Stimuliatsiia immunnogo otveta na immobilizovannyi antigen v sisteme in vitro B-aktivinom.

The influence of B-activin, the preparation of immunomodulating myelopeptides, on the level of antibody formation after the primary immunization of mouse splenocyte cultures with immobilized antigens has been studied. The treatment of the cells with B-activin on the third day of their cultivation in the presence of peroxidase immobilized on polystyrene or protein M1 of influenza virus has been found to increase antigen-specific antibody formation by several times, while having practically no effect on the total level of IgG secretion. The stable level of the stimulation of antibody formation and the possibility of its quantitative evaluation in the enzyme-linked immunosorbent assay makes this immune response inducing system a convenient model for testing the biological activity of myelopeptides and other immunostimulators.