Assuntos
Doadores de Sangue , Preservação de Sangue/normas , Eritrócitos/fisiologia , Hemólise/fisiologia , Desenho de Equipamento , Eritrócitos/diagnóstico por imagem , Humanos , Fragilidade Osmótica/fisiologia , Controle de Qualidade , U.R.S.S. , Ultrassonografia/instrumentação , Ultrassonografia/métodosRESUMO
The effect of ultrasound (frequency 0.88 MHz, intensity from 0.05 to 1 W/cm2) on alterations in antigenic activity has been investigated in vitro using ABO antigens of human erythrocytes. The existence of threshold doses of ultrasound influence has been found. These doses are shown to be independent of ultrasound intensity. The dependence of the effect on erythrocyte concentration has been established. Individual and group differences in the antigenic resistance to ultrasonic exposure in donors of groups A and B have been revealed. A drop in antigenic activity equal to 97% has been obtained.
Assuntos
Sistema ABO de Grupos Sanguíneos/imunologia , Epitopos/análise , Membrana Eritrocítica/imunologia , Sonicação , Ultrassom , Humanos , Técnicas In VitroRESUMO
A technique of antierythrocyte antibody detection is described. It as based on co-agglutination of red blood cells and S. aureus cells. The method is unsophisticated and non-laborious. It provides massive agglutination, its sensitivity is one order of magnitude higher than that of the Coombs test.
Assuntos
Autoanticorpos/análise , Eritrócitos/imunologia , Testes de Hemaglutinação/métodos , Anemia Hemolítica Autoimune/imunologia , Humanos , Staphylococcus aureus/imunologiaAssuntos
Anemia Hemolítica Autoimune/diagnóstico , Pré-Escolar , Feminino , Humanos , Lactente , Recém-Nascido , MasculinoAssuntos
Anemia Hemolítica Congênita/sangue , Membrana Eritrocítica/imunologia , Eritrócitos/imunologia , Proteínas de Membrana/imunologia , Adulto , Eletroforese das Proteínas Sanguíneas , Eletroforese em Gel de Poliacrilamida , Testes de Hemaglutinação/métodos , Humanos , Imunoglobulina M/isolamento & purificação , Masculino , Proteínas de Membrana/análiseAssuntos
Colestase/sangue , Eritrócitos , Adulto , Feminino , Humanos , Masculino , Pessoa de Meia-IdadeRESUMO
A new test for detection of antierythrocyte antibodies is described; one of its variants is three orders of magnitude more sensitive than the classic Coombs test. The proposed procedures are based on the agglutination of the red blood cells following addition of test-erythrocytes coated with the aggregated proteins of an antiglobulin serum. Results of application of the technique for diagnosis of the autoimmune hemolytic anemia showed its high specificity and the absence of prozone phenomenon.