Effect of α1-adrenomimetic indralin on oxygen consumption by bone marrow cells in vitro.

In vitro experiments with excessive and normal oxygenation of the culture medium showed unchanged oxygen consumption by mouse bone marrow cells under the influence of radioprotector indralin belonging to α1-adrenomimetics (100 µg/ml). After exhaustion of oxygen in the medium below 10 µM and progressive decrease in cellular respiration, indralin stimulated oxygen consumption by bone marrow cells by 1.5 times. The role of the observed effect of indralin in the realization of its radioprotective properties is discussed.

[Recombinant human interleukine-1beta (betaleukine) usage for acute radiation sickness of severe degree treatment at canines].

Recombinant human interleukine-1beta (betaleukune) of Institute of especially pure biopreparation production had been examined as a treatment means at acute radiation disease of severe degree at dogs. Dogs were irradiated totally in doses above the LD95/45. Betaleukine had been administered s/c twice in day in 15 min - 2 h after irradiation. All the dogs, including control ones, received in acute period 8-24/26 days after irradiation antibiotics ampicillin and gentamycin i/m. Betaleukine increased 45 day-survival by 37% at 4 Gy and by 25% at 4.4 Gy. The effect correlated with more high level of nadir and more early beginning the leucocyte number restoration. We observed the regularity at all the dogs that received betaleukine as survived as died, but in the latter case in a lesser degree certainly. Besides the noticed character of leucocytes kinetics had been repeated at all the blood cell types but in the different degree. It had been concluded on the base of these observations that betaleukine acts on hemopoietic stem cells preferentially. The effect is in preventing death of a stem cells part, or in stimulating survived stem cells proliferation, or in both together. Betaleukin can be regarded as a suitable means of urgent pathogenic therapy at radiation accidents.

[Radioprotective capacity of indralin in reducing radiation injury to salivary glands]. / Protivoluchevye svoistva indralina po snizheniiu tiazhesti luchevogo porazheniia sliunnykh zhelez.

Radioprotective capacity of radioprotector indraline (alpha-1(B)-adrenoagonist) was studied by its effect on early displays of a local radiation injury to salivary glands in white rats after X-ray irradiation of an animal head with a dose of 18.7 Gy. Indraline was found to be capable to reduce a radiation injury to parotid glands registered by reduction of gland mass on 6th day after irradiation. In experiments on rats, radioprotective efficiency of indraline (100 mg/kg) in term of DRF is close to 1.5.

[The role of beta-adrenoreceptors in the mechanism of the hemodynamic action and radiation-protective activity of the copolymer of 2-methyl-5-vinylpyridine with 2-methyl-5-vinylpyridinium-N-oxide]. / Izuchenie roli beta-adrenoretseptorov v mekhanizme gemodinamicheskogo deistviia i protivoluchevoi aktivnosti sopolimera 2-metil-5-vinilpiridina s 2-metil-5-vinilpiridinii-N-oksid.

In experiment on anesthetized dogs and cats in was shown that the new water-soluble copolymer initiated depressive reaction characteristic for beta-adrenomimetics. This effect was levelled with the help of non-selective adrenoblockator--propranololum. In experiment on dogs the preliminary treatment with propranololum decreased the therapeutic antiradiation efficiency of the copolymer from 68.4 to 8.3%.

[Experimental study of combined effect of actoprotectors and radioprotectors in radiation injury]. / Eksperimental'noe izuchenie vozmozhnosti sovmestnogo primeneniia ak toprotektorov i radioprotektorov pri luchevom porazhenii organizma.

Combined treatment of mice and dogs with actoprotector bimethyl and radioprotector indralin suppressed radioprotective action of the latter, the surveillance being decreased from 83.3 to 14.3% in dogs and from 70.3 to 5% in mice. At the same time vasodynamic action of indralin (increase of artherial pressure up to 30% from a reference level) did not change.

[Effect of co-polymer 2-methyl-5-vinylpyridine and 2-methyl-5- vinylpyridinium-n-oxide radiation induced myelodepression in mice]. / Vliianie sopolimera 2-metil-5-vinilpiridina s 2-metil-5-vinilpiridinii-n-oksidom na mielodepressiiu u myshei, vyzvannuiu oblucheniem.

On the model of cytopenia induced in mice by irradiation with a dose of 4.0 Gy it was shown that injection of examined copolymer (400 mg/kg) 3 h after irradiation exerts positive effect on haemopoietic system: the decrease of damage and acceleration of repair processes.

[Radioprotective activity and toxicity of copolymer 2-methyl-5-vinyl pyridine with 2-methyl-5-vinylpyridinium-n-oxide]. / Protivoluchevaia aktivnost' i toksichnost' sopolimera 2-metil-5-vinilpiridina s 2-metil-5-vinilpiridinii -n-oksidom.

In experiments in mice, hamsters and dogs therapeutic radioprotective efficiency and toxicity of new water-soluble copolymer were studied. It was found that at intramuscular injection of the copolymer to dogs in a dose of 5 mg/kg 24 h after irradiation with a dose of 3.30 Gy (LD85/45) it showed pronounced therapeutic effect (68.1%). In mice and hamsters, the effect was less pronounced: 42-21% after irradiation with a dose of 8.0 Gy. The copolymer is low toxic substance and according to the State standards of Russian Federation belongs to the fourth class (harmless).

[Radioprotective effectiveness and toxicity of 4,5-disubstituted tryptamines]. / Radiozashchitnaia éffektivnost' i toksichnost' 4,5-dizameshchennykh triptaminov.

In experiments on mice a study was made of different substituents in the 4th position of the indole ring of 5-methoxytryptamines (5-MOT) on toxicity and radioprotective efficiency of the compounds of this class. It was shown that the administration of the amino-group to a mexamine molecule increased the preparation toxicity; the nitro-group somewhat diminished the toxic properties, and the acetylamino group did not change 5-MOT toxicity. A 5-MOT derivative with a nitro group possessed the strongest radioprotective action. The radioprotective efficiency of these compounds persisted for 1-2 h.