[Enhancement of epidermal regeneration by recombinant vaccinia virus growth factor]. / Usilenie épidermal'noi regeneratsii s pomoshch'iu rekombinantnogo faktora rosta virusa ospovaktsiny.

Examining the specific activity has showed that recombinant vaccinia virus growth factor binds to appropriate receptors on the A-431 cell surface and prompts the healing acceleration of degree III burns in rats. This recombinant factor did not demonstrate pyrogenicity or toxicogenicity in tests on rabbits, guinea-pits, noninbred albino mice.

[Cloning and sequencing of genes coding the glycoproteins of antigens A and B of oncogenic strain JM of Marek disease virus (MDV)]. / Klonirovanie i sekvenirovanie genov, kodiruiushchikh glikoproteiny A- i B-antigenov onkogennogo shtamma JM virusa bolezni Mareka (MDV).

The genes encoding the precursors of secretory glycoprotein gp57-65 (A antigen) and glycoprotein complex gp100, gp60, gp48 (B antigen) of virulent strain JM of Marek's disease virus (MDV) have been cloned using PCR of viral DNA. Nucleotide sequences of both genes have been determined and amino acid sequence of the precursor peptides predicted, and compared with the corresponding sequences of other MDV strains. The results will be used for developing methods for the diagnosis of Marek's disease and vaccination against it.

[Cloning proviral DNA sequences of the human T-cell leukemia virus (HTLV-1) from the genome of cultured MT-4 cells]. / Klornirovanie provirusnykh DNK-posledovatel'nostei virusa T-kletochnogo leikoza cheloveka tipa 1 (HTLV-1) iz genoma kul'tury kletok MT-4.

MT-4 cell line is a continuous strain of human T lymphocytes expressing defective noninfective subviral HTLV-1 particles. A fragment of sequence encoding the p24 protein and gene for envelope protein (env) have been obtained from genomic DNA of this culture by polymerase chain reaction. Both HTLV-1 fragments were cloned in bacterial vectors, and the nucleotide sequence of these regions was determined. The cloned DNA fragment encoding the p24 has only four point nucleotide exchanges. Analysis of the env gene structure revealed that the sequence had several amino acid exchanges and two deletions (13 bp and 70 bp).

[Cloning and primary structure determination of genes coding for IE2 and PP150 proteins of human cytomegalovirus]. / Klonirovanie i opredelenie pervichnoi struktury genov belkov IE2 i PP150 tsitomegalovirusa cheloveka.

The epidemiological situation with cytomegalovirus infection in Siberia is still to be studied and serological diagnosis of human cytomegalovirus (HCMV) is not satisfactory. Two regions of HCMV genome (strain AD-169) have been examined for obtaining diagnostic reagents by expression cloning. Using polymerase chain reaction, fragments of gene of the immediate early protein (IE2) and of the region encoding for the entire hydrophilic part (1176 bp) of the large phospoprotein gene (pp150) have been obtained. Both fragments were cloned in bacterial vectors. Analysis of nucleotide sequence showed negligible substitutions in comparison with previously reported sequences for these genes.