RESUMO
By alkylation of uracil, thymine, cytosine, adenine, 6-chloropurine, and 2-amino-6-chloropurine with 5-chloro-1-(4-halogenophenyl)-1-pentanones novel derivatives of nucleic bases were obtained, their physicochemical properties were studied. The influence of synthesized compounds on HIV-1 integrase was investigated.
Assuntos
Inibidores Enzimáticos/química , Infecções por HIV/tratamento farmacológico , Integrase de HIV/química , Relação Estrutura-Atividade , Adenina/química , Alquilação , Citosina/química , Inibidores Enzimáticos/farmacologia , HIV/efeitos dos fármacos , HIV/enzimologia , Infecções por HIV/enzimologia , Infecções por HIV/virologia , Integrase de HIV/metabolismo , Humanos , Purinas/química , Timina/química , Uracila/químicaRESUMO
Novel polymethylene derivatives of nucleic bases containing a phenylketo function in the omega-position of hydrocarbon chain were synthesized by alkylation of uracil, thymine, cytosine, hypoxantine, adenine, and N(2)-isobutyrylguanine with omega-chloro-1 -phenylalkan-1-ones, their physicochemical properties were studied.
Assuntos
Purinas/química , Purinas/síntese química , Pirimidinas/química , Pirimidinas/síntese químicaRESUMO
NS3 protein of hepatitis C virus plays the key role in the virus functioning. It possesses three enzymatic activities, namely protease activity, associated with N-terminal domain of the protein, and helicase/NTPase activities specific for C-terminal domain. Here, the effect of some polimethylenic derivatives of the nucleic bases on helicase and ATPase enzyme activities has been studied. Several of compounds tested displayed inhibitory activity towards NS3 helicase. However, most compounds demonstrated strong activating effect on ATPase activity of the enzyme as well as several other ATPases. The ATPase activating mechanism was not described earlier. The activation potency of the compounds depended on substrate/activator concentration ratio, and was maximal at the 1000:1. The activation mechanism scheme that allows us to explain phenomena observed is proposed.
Assuntos
Hepacivirus/enzimologia , Nucleotídeos/metabolismo , Nucleotídeos/farmacologia , Poliaminas/metabolismo , Poliaminas/farmacologia , Proteínas não Estruturais Virais/agonistas , Proteínas não Estruturais Virais/antagonistas & inibidores , Adenosina Trifosfatases/metabolismo , Ativação Enzimática , Humanos , Nucleotídeos/química , Poliaminas/química , RNA Helicases/antagonistas & inibidores , RNA Helicases/metabolismo , Proteínas não Estruturais Virais/metabolismoRESUMO
New polymethylene derivatives of nucleic bases with a beta-diketo function in the omega-position were obtained by alkylation of uracil, thymine, cytosine, hypoxanthine, adenine, and N(2)-isobutyryl guanine with 2-omega-chloroal-kanoyl)cyclohexanones. The physical and chemical characteristics of the compounds synthesized and their effect on the K562 and HCT116 tumor cell lines were studied.
Assuntos
Antineoplásicos/farmacologia , Proliferação de Células/efeitos dos fármacos , Purinas/farmacologia , Pirimidinas/farmacologia , Alquilação , Antineoplásicos/química , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Citosina/farmacologia , Doxorrubicina/farmacologia , Interações Medicamentosas , Resistencia a Medicamentos Antineoplásicos , Ensaios de Seleção de Medicamentos Antitumorais , Humanos , Hipoxantina/farmacologia , Purinas/química , Pirimidinas/química , Relação Estrutura-Atividade , Timina/farmacologia , Uracila/farmacologiaRESUMO
New polymethylene derivatives of nucleic bases with beta-diketo function in omega-position were prepared by alkylation of uracil, thymine, and cytosine. Their physicochemical properties and effect on the E. coli uridine phosphorylase were studied.
Assuntos
Escherichia coli/enzimologia , Ácidos Nucleicos/química , Pirimidinas/química , Uridina Fosforilase/antagonistas & inibidores , Ácidos Nucleicos/síntese química , Pirimidinas/síntese química , Uridina Fosforilase/químicaRESUMO
New polymethylene derivatives of nucleic bases containing a keto function in the omega-position were synthesized by alkylation of nucleic bases with 2-(3-chloropropyl)-2-phenyl-1,3-dioxolane and the subsequent deblocking of the keto group; their physicochemical properties were studied. The English version of the paper: Russian Journal of Bioorganic Chemistry, 2005, vol. 31, no. 6; see also http://www.maik.ru.
Assuntos
Butirofenonas/química , Nucleosídeos/química , Purinas/química , Pirimidinas/química , Butirofenonas/síntese química , Purinas/síntese química , Pirimidinas/síntese químicaRESUMO
New polymethylene derivatives of nucleic bases containing a beta-dioxo function at the omega-position were synthesized by alkylation of uracil, thymine, and cytosine with 1-(7-chloroheptanoyl)cyclohexan-2-one, and their physicochemical properties were studied. The English version of the paper: Russian Journal of Bioorganic Chemistry, 2004, vol. 30, no. 5; see also http: // www.maik.ru.
Assuntos
Bioquímica/métodos , Metano/análogos & derivados , Nucleosídeos/química , Pirimidinas/química , Alquilação , Citosina/química , Hidrocarbonetos , Metano/química , Timina/química , Uracila/químicaRESUMO
A five-step synthesis of alpha-methylspermidine (1,8-diamino-5-azanonane), the first polyamine analogue preventing pathological consequences of spermidine depletion in transgenic rats overproducing spermine/spermidine N'-acetyltransferase, from ethyl 3-aminobutyrate was achieved in a high overall yield.
Assuntos
Espermidina/análogos & derivados , Espermidina/síntese química , Animais , Animais Geneticamente Modificados , Espectroscopia de Ressonância Magnética/métodos , Ratos , Espermidina/químicaRESUMO
N9-Polymethylene derivatives of adenine and hypoxanthine with various functional groups in the omega-position of the alkyl substituent were synthesized. Their physico-chemical properties and effect on the HIV reverse transcriptase and DNA topoisomerase I were studied.
Assuntos
Adenina/análogos & derivados , Hipoxantinas , Metano/análogos & derivados , Adenina/síntese química , Adenina/farmacologia , Alquilação , DNA Topoisomerases Tipo I/efeitos dos fármacos , Transcriptase Reversa do HIV/efeitos dos fármacos , Hidrocarbonetos , Hipoxantina/síntese química , Hipoxantina/farmacologiaRESUMO
N1-Acyclic derivatives of pyrimidine bases (uracil, thymine, and cytosine) with hydrophobic polymethylene chains containing various functional groups in an omega-position of the alkyl substituent were synthesized. Their physicochemical properties and inhibitory effect on the HIV reverse transcriptase and human DNA topoisomerase I were studied.
Assuntos
Citosina/análogos & derivados , Citosina/síntese química , Timina/análogos & derivados , Timina/síntese química , Uracila/análogos & derivados , Uracila/síntese química , Citosina/química , DNA Topoisomerases Tipo I/química , Inibidores Enzimáticos/síntese química , Inibidores Enzimáticos/química , Transcriptase Reversa do HIV/antagonistas & inibidores , Transcriptase Reversa do HIV/química , Humanos , Inibidores da Transcriptase Reversa/síntese química , Inibidores da Transcriptase Reversa/química , Relação Estrutura-Atividade , Timina/química , Inibidores da Topoisomerase I , Uracila/químicaRESUMO
The temperature dependence of CD spectra of ApA, its (2'--5')-isomer, and a number of free conformational analogues, in which the ribose ring was replaced by acyclic hydroxyalkyl substituents, has been studied. The thermodynamic parameters of conformational equilibrium were determined. The role of separate functional groups and parts of DNP molecules in the organization and stabilization of the close state as well as the role of water in the stabilization of the one stranded helix of RNA is discussed.
Assuntos
Monofosfato de Adenosina/análogos & derivados , Fosfatos de Dinucleosídeos , Fenômenos Químicos , Química , Dicroísmo Circular , Isomerismo , Conformação Molecular , TermodinâmicaRESUMO
The strategy of quantitative study of conformational situation in aqueous solutions of dinucleoside phosphates (DNP) has been proposed. It includes the following steps: (1) conformational calculation of stacking conformers (SC) of DNP; (2) measurement of thermodynamic parameters of conformational equililbrium; (3) determination of extensive parameters of stacking state (SS) on the basis of experimental data and thermodynamic parameters; (4) computation of extensive parameters of SC on the basis of the calculated geometry of SC and theoretical concepts of experimental method; (5) comparison of experimental parameters of SS with the calculated ones of SC to establish the SC participating in the equilibrium as well as its relative weights. This strategy was exemplified by ApA, ApC, CpA and CpC. The chemical shifts, spin-spin coupling constants and spin-lattice relaxation rates of DNP protons were used. The predominant SC was found to be related to Pba type with a large twirl angle (45-54 degrees) and almost parallel base planes. For all DNP the presence in equilibrium of left-handed forms was typical. These were Mab forms for ApA, Mbb forms for CpC and CpA, Mbb and Mab forms for ApC. About 10% of SS of ApA and CpA were presented by SC with trans-conformation of C(4')-C(5') bond of oN-part. The SC with C(3')-endo, C(3')-endo combination of ribose conformations were basic for ApA, ApC and CpA, and the only possible for CpC. In case of ApA, ApC and CpA the conformers with C(3')-endo, C(2')-endo sugar combination were presented.
Assuntos
Nucleotídeos , Fenômenos Químicos , Química , Espectroscopia de Ressonância Magnética , Modelos Químicos , Conformação Molecular , Soluções , Termodinâmica , ÁguaRESUMO
The dinucleoside phosphates analogues APpSU, SUPpA and UPpSU have been synthesised by direct thionation of DNP analogues, containing cytosine. The structure of compounds prepared was proved by UV- and PMR-spectra. The CD spectra of the DNP analogues were examined. It has been demonstrated that the "induced" dichroism contribution in the Cotton-effect of DNP was substantial in the case of stacking conformations and negligible for unstacking conformations.
Assuntos
Nucleotídeos , Fenômenos Químicos , Química , Dicroísmo Circular , Conformação Molecular , Nucleotídeos/síntese química , Temperatura , TiouracilaRESUMO
The dependence of the NMR spectra of adenosine 5'-phosphate and phosphates of 9-(2'-hydroxyethyl)-, 9-(3'-hydroxypropyl)- and 9-(4'-hydroxybutyl)adenines on temperature and concentration has been investigated in aqueous solutions.
Assuntos
Nucleotídeos de Adenina , Monofosfato de Adenosina , Espectroscopia de Ressonância Magnética , Relação Estrutura-AtividadeRESUMO
Circular dichroism spectra of 11 analogues of the dinucleoside phosphate containing achiral 3'-terminal monomers have been measured at several pH values, various temperatures and various concentrations of ethanol. The conformation of analogues studied has been shown to by very similar to that of natural compounds. Comparison of the results obtained with the circular dichroism spectra of the corresponding natural compounds indicates that Cotton effect arises from monomeric circular dichroism, at least in main features. The exciton interaction is relatively small.
Assuntos
Nucleosídeos , Fenômenos Químicos , Química , Dicroísmo Circular , Concentração de Íons de HidrogênioRESUMO
On the basis of the application of transition-state theory to enzymatic catalysis a novel approach to the synthesis of inhibitors of enzymes involving conformational rigid substrate has been proposed. It is predicted that the substrates which resemble the natural species in the structure of functional groups, but posses conformational flexibility, will be bound to the enzyme more tightly and converted more slowly than the natural substrate.