RESUMO
The Rancourt EST Database (RED) is a web-based system for the analysis, management, and dissemination of expressed sequence tags (ESTs). RED represents a flexible template DNA sequence database that can be easily manipulated to suit the needs of other laboratories undertaking mid-size sequencing projects.
Assuntos
Sistemas de Gerenciamento de Base de Dados , Bases de Dados de Ácidos Nucleicos , Etiquetas de Sequências Expressas , Análise de Sequência de DNA/métodos , Software , Animais , Sequência de Bases , Humanos , Armazenamento e Recuperação da Informação/métodos , Dados de Sequência MolecularRESUMO
A rate-determining step in gene targeting is the generation of the targeting vector. We have developed bacteriophage gene targeting vectorology, which shortens the timeline of targeting vector construction. Using retro-recombination screening, we can rapidly isolate targeting vectors from an embryonic stem cell genomic library via integrative and excisive recombination. We have demonstrated that recombination can be used to introduce specific point mutations or unique restriction sites into gene targeting vectors via transplacement. Using the choline/ethanolamine kinase alpha and beta genes as models, we demonstrate that transplacement can also be used to introduce specifically a neo resistance cassette into a gene targeting phage. In our experience, the lambdaTK gene targeting system offers considerable flexibility and efficiency in TV construction, which makes generating multiple vectors in one week's time possible.
Assuntos
Bacteriófago lambda/genética , Marcação de Genes/métodos , Mutagênese Insercional/métodos , Colina Quinase/genética , Vetores Genéticos , Fosfotransferases (Aceptor do Grupo Álcool)/genética , Plasmídeos/genética , Recombinação GenéticaRESUMO
Neurodegenerative diseases cost the economies of the developed world billions of dollars per annum. Given ageing population profiles and the increasing extent of this problem, there has been a surge of interest in neural stem cells and in neural differentiation protocols that yield neural cells for therapeutic transplantation. Due to the oncogenic potential of stem cells a better characterisation of neural differentiation, including the identification of new neurotrophic factors, is required. Stem cell cultures undergoing synchronous in vitro neural differentiation provide a valuable resource for gene discovery. Novel tools such as microarrays promise to yield information regarding gene expression in stem cells. With the completion of the yeast, C. elegans, Drosophila, human, and mouse genome projects, the functional characterisation of genes using genetic and bioinformatic tools will aid in the identification of important regulators of neural differentiation.
Assuntos
Sistema Nervoso/citologia , Neurônios/citologia , Células-Tronco/citologia , Idoso , Animais , Transplante de Tecido Encefálico , Diferenciação Celular , Linhagem da Célula , Movimento Celular , Transplante de Células , Células Cultivadas , Perfilação da Expressão Gênica , Terapia Genética , Genômica , Humanos , Camundongos Mutantes Neurológicos , Fatores de Crescimento Neural/farmacologia , Fatores de Crescimento Neural/fisiologia , Proteínas do Tecido Nervoso/genética , Crista Neural/citologia , Doenças Neurodegenerativas/patologia , Doenças Neurodegenerativas/terapia , Neuroglia/citologia , TransfecçãoRESUMO
We have used a method for synchronously differentiating murine embryonic stem (ES) cells into functional neurons and glia in culture. Using subtractive hybridization we isolated approximately 1200 cDNA clones from ES cell cultures at the neural precursor stage of neural differentiation. Pilot studies indicated that this library is a good source of novel neuro-embryonic cDNA clones. We therefore screened the entire library by single-pass sequencing. Characterization of 604 non-redundant cDNA clones by BLAST revealed 96 novel expressed sequence tags (ESTs) and an additional 197 matching uncharacterized ESTs or genomic clones derived from genome sequencing projects. With the exception of a handful of genes, whose functions are still unclear, most of the 311 known genes identified in this screen are expressed in embryonic development and/or the nervous system. At least 80 of these genes are implicated in disorders of differentiation, neural development and/or neural function. This study provides an initial snapshot of gene expression during early neural differentiation of ES cell cultures. Given the recent identification of human ES cells, further characterization of these novel and uncharacterized ESTs has the potential to identify genes that may be important in nervous system development, physiology and disease.
Assuntos
Embrião de Mamíferos/citologia , Etiquetas de Sequências Expressas , Camundongos/genética , Sistema Nervoso/citologia , Sistema Nervoso/embriologia , Células-Tronco/citologia , Animais , Diferenciação Celular , Sistema Nervoso Central/embriologia , Dados de Sequência Molecular , Proteínas do Tecido Nervoso/genética , Neuroglia/citologia , Neurônios/citologiaRESUMO
Family ZMK is a large Irish kindred that segregates progressive sensorineural hearing loss and retinitis pigmentosa. The symptoms in the family are almost identical to those observed in Usher syndrome type III. Unlike that in Usher syndrome type III, the inheritance pattern in this family is compatible with dominant, X-linked dominant, or maternal inheritance. Prior linkage studies had resulted in exclusion of most candidate loci and >90% of the genome. A tentative location for a causative nuclear gene had been established on 9q; however, it is notable that no markers were found at zero recombination with respect to the disease gene. The marked variability in symptoms, together with the observation of subclinical muscle abnormalities in a single muscle biopsy, stimulated sequencing of the entire mtDNA in affected and unaffected individuals. This revealed a number of previously reported polymorphisms and/or silent substitutions. However, a C-->A transversion at position 12258 in the gene encoding the second mitochondrial serine tRNA, MTTS2, was heteroplasmic and was found in family members only. This sequence change was not present in 270 normal individuals from the same ethnic background. The consensus C at this position is highly conserved and is present in species as divergent from Homo sapiens as vulture and platypus. The mutation probably disrupts the amino acid-acceptor stem of the tRNA molecule, affecting aminoacylation of the tRNA and thereby reducing the efficiency and accuracy of mitochondrial translation. In summary, the data presented provide substantial evidence that the C12258A mtDNA mutation is causative of the disease phenotype in family ZMK.
Assuntos
DNA Mitocondrial/genética , Ligação Genética/genética , Perda Auditiva Neurossensorial/genética , Mutação Puntual/genética , RNA de Transferência de Serina/genética , Retinose Pigmentar/genética , Animais , Sequência de Bases , Cromossomos Humanos Par 9/genética , Herança Extracromossômica/genética , Feminino , Variação Genética/genética , Haplótipos/genética , Perda Auditiva Neurossensorial/patologia , Humanos , Irlanda , Masculino , Mitocôndrias/genética , Mitocôndrias/patologia , Anormalidades Musculoesqueléticas/genética , Anormalidades Musculoesqueléticas/patologia , Linhagem , Polimorfismo Conformacional de Fita Simples , Retinose Pigmentar/patologia , Alinhamento de SequênciaRESUMO
Glaucoma describes a clinically and genetically heterogeneous group of diseases that result in optic neuropathy and progressive loss of visual fields. A gene for juvenile onset primary open angle glaucoma JOAG) has recently been mapped to 1q21-31. Mutations in the trabecular meshwork induced glucocorticoid response gene (TIGR, also known as myocilin or the GLC1A locus) have been found to cause both juvenile and later onset primary open angle glaucoma. Family TCD-POAG1 is a Spanish kindred, which segregates JOAG in an autosomal dominant fashion. This family was found to be linked to the previously identified GLC1A locus on chromosome 1q. Direct sequencing of the TIGR/myocilin gene showed a heterozygous A to C transition in codon 380, resulting in the substitution of alanine for aspartic acid (Asp380Ala). This substitution created a StyI restriction site, which segregated with the JOAG phenotype and permitted rapid screening of all members of the family. This restriction site was not present in 60 controls.
Assuntos
Alanina/genética , Ácido Aspártico/genética , Proteínas do Olho/genética , Glaucoma de Ângulo Aberto/genética , Glicoproteínas/genética , Mutação Puntual , Adolescente , Idade de Início , Proteínas do Citoesqueleto , Feminino , Humanos , Masculino , LinhagemRESUMO
A gene for juvenile onset, open angle glaucoma (JOAG) has been localized to chromosome 1q21-31 in several families. Mutations in the trabecular meshwork-induced glucocorticoid response protein (TIGR) gene, which maps to this region, recently have been found in families segregating both JOAG and a later onset form of primary open angle glaucoma (POAG). We have analysed the TIGR gene in two families; one Spanish family segregating autosomal dominant JOAG and an Irish family with a later onset form of autosomal dominant POAG. We have found a G-T transversion in the first base of codon 426 in all affected members of the Spanish family, which results in a valine to phenylalanine amino acid substitution. We have also found a G-A transition at the first base of codon 367 that segregates through all but one branch of the Irish family and results in a glycine to arginine amino acid substitution. Members of this family that carry the Gly367Arg change also share a common haplotype that is neither present in any of the unaffected members of the family, nor in the branch that does not segregate the mutation. Identification of further mutations in the TIGR gene increases its importance in the etiology of open angle glaucoma.
Assuntos
Proteínas do Olho/genética , Glaucoma de Ângulo Aberto/genética , Glicoproteínas/genética , Mutação Puntual/genética , Adulto , Idade de Início , Idoso , Cromossomos Humanos Par 1/genética , Proteínas do Citoesqueleto , Análise Mutacional de DNA , Feminino , Genes Dominantes/genética , Ligação Genética , Haplótipos , Humanos , Irlanda , Masculino , Linhagem , Polimorfismo Conformacional de Fita Simples , EspanhaRESUMO
We have located three extended families in Ireland (population 3.5 million) with autosomal dominant simplex forms of Epidermolysis Bullosa (EBS). A mutation within the keratin type I (K14) gene (Met-->272-->Arg) in one family suffering from the generalized simplex (Koebner) form of the disease has been previously described (Humphries et al., Hum Mutat 2:37-42, 1993). Here we report on the identification of mutations within the remaining two families, both of whom suffer from the Weber-Cockayne form of the disease. These mutations, within the type II keratin (K5) gene, are Asn-->193-->Lys and Met-->327-->Thr. They have been shown in each case to co-segregate with the disease and are not present in the normal population. Within the three families, a total of 44 living persons with such mutations have been identified, providing a minimum prevalence estimate for the disease in the Irish population of approximately 1 in 80,000, compared to an overall estimated global incidence at birth for all forms of EB of 1 in 50,000. Therefore, these three mutations probably account for the majority of cases of EBS within this population.
Assuntos
Epidermólise Bolhosa Simples/genética , Genes Dominantes , Queratinas/genética , Mutação Puntual , Sequência de Aminoácidos , Sequência Conservada , Epidermólise Bolhosa Simples/etnologia , Feminino , Humanos , Irlanda , Masculino , Dados de Sequência Molecular , LinhagemRESUMO
Best's vitelliform macular dystrophy is an early onset, autosomal dominant macular degeneration. Linkage analysis has previously mapped a disease locus in this disorder to the pericentromeric region of chromosome 11. We examined two families, one of German and one of Irish origin, both affected with this disorder. The Irish family (BTMD1) showed strong evidence for linkage to the previously reported locus on chromosome 11. Linkage of the disease locus to the same region of chromosome 11 has been significantly excluded in the German family (Fam E), thereby providing evidence of locus heterogeneity in this clinically unique condition.
Assuntos
Genes Dominantes , Degeneração Macular/genética , Cromossomos Humanos Par 11/genética , Feminino , Heterogeneidade Genética , Ligação Genética , Marcadores Genéticos , Alemanha , Humanos , Irlanda , Degeneração Macular/classificação , Degeneração Macular/epidemiologia , Masculino , LinhagemRESUMO
We have identified a single base change in exon 4 of the type I keratin gene which results in the replacement of a methionine for an arginine residue at codon 272 in an Irish family displaying an autosomal dominant simplex (Koebner) form of epidermolysis bullosa (EB). This family had previously provided tentative evidence for linkage to genetic markers on chromosome 1q. The mutation cosegregates with the disease, producing a lod score of 4.8 at theta = 0.