RESUMO
Digital imaging is the method of choice for documentation and analysis of electrophoretic separations of protein and DNA. Digital images of gel electropherograms can be obtained rapidly using sCMOS and CCD-based cameras, and the images can be easily archived and analyzed using image analysis software. This overview defines important key terms and calculations for imaging, explains the capture process, reviews the range of technologies used for image capture, and provides an introduction to the software and methods used for one- and two-dimensional digital image analysis. © 2023 Wiley Periodicals LLC.
Assuntos
Processamento de Imagem Assistida por Computador , Software , Eletroforese/métodos , Processamento de Imagem Assistida por Computador/métodos , DNA/análise , Proteínas/análiseRESUMO
"Camera Technologies for Low Light Imaging" is designed to offer the reader a summary of the current camera choices for low light imaging with special emphasis on the practical aspects related to each. Four major camera technologies, cooled charge-coupled device, cooled sCMOS, intensified cameras, and electron multiplier cameras, are discussed and compared. Supporting information about noise characteristics of photoelectrons and camera noise are provided and used for illuminating the practical aspects of using these cameras.
Assuntos
Luz , Fotografação/instrumentação , Algoritmos , Humanos , Aumento da Imagem/métodos , Razão Sinal-RuídoRESUMO
Gel electrophoresis has become a ubiquitous method in molecular biology for separating biomolecules. This prominence is the result of several factors, including the robustness, speed, and potentially high throughput of the technique. The results of this method are traditionally documented using silver halide-based photography followed by manual interpretation. While this remains an excellent method for qualitative documentation of single-gel results, digital capture offers a number of significant advantages when documentation requires quantitation and sophisticated analysis. Digital images of gel electropherograms can be obtained rapidly using an image-capture device, and the images can be easily manipulated using image analysis software. This overview presents reasons for digital documentation and analysis, defines some important key terms for imaging, explains the capture process and reviews the devices used for image capture, and provides an introduction to the software and methods used for one- and two-dimensional digital image analysis.
Assuntos
Eletroforese/métodos , Documentação , Eletroforese/economia , Reprodutibilidade dos TestesRESUMO
Gel electrophoresis has become a ubiquitous method in molecular biology for separating biomolecules. This prominence is the result of several factors, including the robustness, speed, and potentially high throughput of the technique. The results of this method are traditionally documented using silver halide-based photography followed by manual interpretation. While this remains an excellent method for qualitative documentation of single-gel results, digital capture offers a number of significant advantages when documentation requires quantitation and sophisticated analysis. Digital images of gel electropherograms can be obtained rapidly using an image-capture device, and the images can be easily manipulated using image analysis software. This overview presents reasons for digital documentation and analysis, defines some important key terms for imaging, explains the capture process and reviews the devices used for image capture, and provides an introduction to the software and methods used for one- and two-dimensional digital image analysis.