RESUMO
Three patients developed Q fever endocarditis on porcine bioprosthetic valves. They had a subacute or chronic course with nonspecific symptoms, enlargement of the liver and spleen, and cardiac failure due to destruction of the cusps, without disruption of the valve ring. High-phase I-specific IgG and IgA antibody titers against Coxiella burnetii were found. C. burnetii was isolated in each patient by inoculating suspensions of valve tissue into a human fetal diploid fibroblast cell line, which was grown as monolayers on slides contained inside rubber-stoppered tube cultures. Patients were treated successfully with doxycycline, cotrimoxazole, and valve replacement and were followed up for periods of 24 to 42 months; no evidence of deterioration was found. The human fetal diploid cell culture may be an expeditious, easy, and safe method to isolate C. burnetii from cardiac valves. Valve replacement seemed necessary to cure prosthetic-valve endocarditis due to C. burnetii infection. Combined therapy with doxycycline and cotrimoxazole may control the disease and prevent reinfection of the homografts replacing the valves.
Assuntos
Bioprótese , Endocardite Bacteriana , Próteses Valvulares Cardíacas , Febre Q , Adulto , Coxiella/isolamento & purificação , Doxiciclina/uso terapêutico , Combinação de Medicamentos/uso terapêutico , Endocardite Bacteriana/tratamento farmacológico , Endocardite Bacteriana/microbiologia , Endocardite Bacteriana/patologia , Feminino , Valvas Cardíacas/patologia , Humanos , Masculino , Febre Q/tratamento farmacológico , Febre Q/microbiologia , Febre Q/patologia , Reoperação , Sulfametoxazol/uso terapêutico , Trimetoprima/uso terapêutico , Combinação Trimetoprima e SulfametoxazolRESUMO
Chromosome studies were performed on a human glioma cell line. Nineteen passages were studied, and a heterogeneous chromosome complement with variable modal numbers was found. The main range varied from tetraploidy to triploidy. The presence of identical markers (9p-, 11p+, an increase in the copies of chromosome No. 7) in different passages suggests a clonal evolution.
Assuntos
Neoplasias Encefálicas/genética , Aberrações Cromossômicas , Glioma/genética , Linhagem Celular , HumanosRESUMO
A simple method is described for obtaining images in relief of fresh preparations with an ordinary light microscope by lowering the condenser and displacing the objective at an "off-clicked" position.