Acclimatizing waste activated sludge in a thermophilic anaerobic fixed-bed biofilm reactor to maximize biogas production for food waste treatment at high organic loading rates.

Thermophilic anaerobic digestion (TAD) provides a promising solution for sustainable high-strength waste treatment due to its enhanced methane-rich biogas recovery. However, high organic loading rates (OLR) exceeding 3.0 kgCOD/m3/day and short hydraulic retention times (HRT) below 10 days pose challenges in waste-to-energy conversion during TAD, stemming from volatile fatty acids (VFAs) accumulation and methanogenesis failure. In this study, we implemented a stepwise strategy for acclimatizing waste activated sludge (WAS) in a thermophilic anaerobic fixed-bed biofilm reactor (TA-FBBR) to optimize methanogen populations, thereby enhancing waste-to-energy efficiencies under elevated OLRs in food waste treatment. Results showed that following stepwise acclimatization, the TA-FBBR achieved stable methane production of approximately 5.8 L/L-reactor/day at an ultrahigh OLR of ∼20 kgCOD/m3/day and ∼15 kgVS/m3/day at 6-day HRT in food waste treatment. The average methane yield reached 0.45 m3/kgCODremoval, attaining the theoretical production in TAD. Moreover, VFA concentrations were stabilized below 1000 mg/L at the ultrahigh OLR under 6-day HRT, while maintaining an acetate/propionate ratio of > 1.8 and a VFA/TAK ratio of < 0.3 serving as effective indicators of system stability and methane yield potential. The microbial community analysis revealed that the WAS acclimatization strategy fostered the microbial diversity and abundance of Methanothermobacter and Methanosarcina. Methanosarcina in the biofilm were observed to be twice as abundant as Methanothermobacter, indicating a potential preference for biofilm existence among methanogens. The findings demonstrated an effective strategy, specifically the stepwise acclimatization of WAS in a thermophilic fixed-bed biofilm reactor, to enhance the food waste treatment performance at high OLRs, contributing valuable mechanistic and technical insights for future sustainable high-strength waste management.

Enrichment of thermophilic methanogenic microflora from mesophilic waste activated sludge for anaerobic digestion of garbage slurry.

This study investigated a startup strategy for thermophilic methanogenic enrichment. Conventional waste activated sludge (WAS) was used as the seed. The WAS seed was incubated at 55 °C in a continuous-flow stirred tank reactor, with garbage slurry fed continuously as a substrate. One of the two reactors (termed reactor-high, RH) was fed with a high concentration of substrate (30 g-COD/L), while the other (reactor-low, RL) received a lower concentration of feed (15 g-COD/L). The specific organic loading rate was 0.2 g-COD/L/day initially, which was gradually increased by shortening the hydraulic retention time. The final OLR was 3.2 g-COD/L/day, after more than 90% of the initial WAS got washed out from the reactor and thermophilic microorganisms became dominant in the reactors. Biogas production rate and methane conversion ratio depended on substrate concentration, although total chemical oxygen demand removal and methane content were almost the same in RH and RL. Biogas production rate in RH was 3.2 times higher than that in RL, while the conversion ratio of RH was 1.6 times higher than that of RL. Quantitative polymerase chain reaction analysis using specific primers for the mcrA gene and high-throughput sequencing analysis of 16S rRNA gene amplicons demonstrated post enrichment differences in the microbial community, relative to that in the WAS. There was no significant difference in the enriched microbial community composition between RH and RL. In conclusion, thermophilic methanogenic microflora can be enriched from mesophilic seeds. Methanothermobacter, Methanosarcina, and other thermophilic bacteria were enriched in the community over time, with these thermophiles collectively accounting for ∼80% of the stable thermophilic community.