Economic losses caused by mastitis and the influence of climate variation on the occurrence of the disease in a dairy cattle farm in southern Brazil.

This study evaluated the economic impacts caused by mastitis in a small dairy farm with similar characteristics and production to most dairy farms in southern Brazil and investigated if climatic variations influenced mastitis occurrence in the region. A farm with, on average, 45 lactating Holstein cattle was monitored from November 2021 to October 2022, and data on mastitis cases, bulk tank milk somatic cell count, animal treatment costs, milk production, animal disposal costs, and production losses were collected. Monthly averages of temperature, relative humidity (RH), and rainfall in the region were obtained. The greatest loss was related to the drop in milk production, resulting in 63.8% of total losses, followed by animal disposal (29.5%), milk disposal (4.6%), and treating animals with mastitis (2.0%), totaling a 10.6% reduction in the annual gross income. There were negative correlations between the clinical mastitis rate and monthly RH and between subclinical mastitis and temperature; the occurrence of subclinical mastitis and average RH were positively correlated. Our findings showed that mastitis negatively impacted the economy and that climate influenced mastitis occurrence.

Efficacy Evaluation of a Commercial Formulation With Duddingtonia Flagrans in Equine Gastrointestinal Nematodes.

The indiscriminate use of antiparasitics for the treatment of helminths in horses has caused the ineffectiveness of commonly used chemical active principles, therefore, new alternatives such as the use of helminthophagous fungi have been studied. In this context, this study aimed to evaluate the in vitro efficacy of the commercial formulation Bioverm, composed of the fungus Duddingtonia flagrans strain AC001, in the reduction of gastrointestinal nematode larvae in equine feces. In coproculture, the genus Cyathostomum sp. was the most prevalent in the analyzed samples. The commercial formulation with D. flagrans demonstrated effectiveness in the predation of Cyathostomum sp. in tests. The recommended dose of 0.4 g, containing 105 chlamydospores per gram of product, reduced larvae by 44.23%, while the extrapolated dose of 1.0 g with the same concentrations of chlamydospores (105/g) resulted in a reduction of 57.20%, indicating the effectiveness of the product in controlling infective larvae.

Isolation and characterization of Buchananella hordeovulneris from feline pyothorax.

A 1-year-old female mixed-breed cat was admitted to a veterinary hospital in Southern Brazil with tachypnea, low thoracic amplitude, restrictive breathing pattern, and cyanotic mucous membranes 2 days after elective castration surgery. Radiography revealed pleural effusion, and approximately 100-200 mL of fluid was collected by thoracocentesis. The reddish purulent exudate contained large numbers of yellowish-white granules with branched filamentous structures on cytological examination. The fluid was plated on blood agar and incubated under aerobiosis at 37 °C. On the third day of incubation, circular, dry, and opaque colonies, measuring < 0.5 mm in diameter, were observed. Their phenotypic and molecular characteristics were compatible with Buchananella hordeovulneris (basonym: Actinomyces hordeovulneris), a pathogenic actinomycete rarely detected in cats. Our findings indicate that B. hordeovulneris should be included in the differential diagnosis of pyothorax in cats together with Actinomyces spp. and Nocardia spp. Taxonomic confirmation of disease-causing microorganisms in animals is important to understand the course of infection and its association with disease epidemiology.