RESUMO
A 21-year-old multiparous female exhibiting 31-41 day menstrual cycles was given hFSH (225 IU/day, Metrodin 75, from cycle day 3 through 9 (menses = day 1) and hCG (10,000 IU, Profasi, on day 10 to stimulate follicular development. At 35 h after hCG, under isoflurane (AErrane) anesthesia, follicles were aspirated by controlled suction under transvaginal ultrasound guidance. Metaphase II oocytes (n = 11) were placed in modified human tubal fluid (mHTF, 100 microliters) medium under oil at 37 degrees C in humidified 5% CO2. Frozen semen, collected by voluntary ejaculation, was thawed (70 degrees C H2O bath, 6 sec), diluted slowly, centrifuged, and resuspended in mHTF, and 160,000 motile spermatozoa/ml were added at 6 h after oocyte recovery. At 21 h postinsemination (p.i.) eight oocytes were at the two-cell stage, five were cryopreserved, and three were cultured to the six- to eight-cell stage in mHTF with granulosa cells before transcervical uterine transfer at 47 h p.i. using a Teflon catheter. Micronized progesterone (400 mg/d) was orally administered for 10 weeks posttransfer (p.t.). Ultrasound examination revealed a single fetus at 15 weeks p.t., and unassisted delivery of a live 1.37 kg female infant occurred at 29 weeks. Am. J. Primatol. 41:247-260, 1997.
Assuntos
Transferência Embrionária/veterinária , Fertilização in vitro/veterinária , Gorilla gorilla , Animais , Criopreservação/veterinária , Transferência Embrionária/métodos , Feminino , Fertilização in vitro/métodos , Hormônio Foliculoestimulante/administração & dosagem , Ciclo Menstrual/fisiologia , Oócitos , Preservação do Sêmen/veterináriaAssuntos
Fenoprofeno/uso terapêutico , Histerossalpingografia/efeitos adversos , Dor/prevenção & controle , Fenilpropionatos/uso terapêutico , Pré-Medicação , Adulto , Assistência Ambulatorial/métodos , Aspirina/uso terapêutico , Método Duplo-Cego , Avaliação de Medicamentos , Feminino , Humanos , Dor/etiologia , Placebos , Distribuição AleatóriaRESUMO
The serum concentration of human chorionic gonadotropin (hCG) was measured at 10- to 60-minute intervals for 4-24 hours in 5 women at 5-8 weeks of pregnancy. hCG was found to be secreted episodically with pulses observed every 2-4 hours. The episodic secretion of hCG may have important implications to our understanding of both normal and abnormal reproduction.