RESUMO
The problem of transgenic plants application to production of antigens, antibodies, enzymes, and hormones is discussed. Transgenic plants are a highly effective system for production of bioactive molecules. Prospects for using transgenic plants expressing viral and bacterial antigens for mucosal immunization are discussed.
Assuntos
Plantas Geneticamente Modificadas/metabolismo , Vacinas , Anticorpos , Antígenos/biossíntese , Enzimas/biossíntese , Hormônios/biossínteseRESUMO
Residual virulence of three vaccinia strains: Neurovaccine, L-IVP, and its recombinant Revacs-B expressing HBs and preS2 antigens of hepatitis B virus is compared. Insertion of HBs and preS2 antigens of hepatitis B virus in the genome of vaccinia virus strain L-IVP decreases its residual virulence and leads to a benign course of vaccinal reaction involving no deaths of rabbits, cotton rats, or guinea pigs. We may expect that recombinant vaccine Revacs B based on L-IVP strain will cause no postvaccinal complications under conditions of an appreciable decrease in population immunity to vaccinia virus.
Assuntos
Vaccinia virus/imunologia , Vacinas Virais/efeitos adversos , Animais , Cobaias , Antígenos de Superfície da Hepatite B/genética , Precursores de Proteínas/genética , Coelhos , Sigmodontinae , Vaccinia virus/patogenicidade , Proteínas do Envelope Viral/genética , Vacinas Virais/genética , Vacinas Virais/metabolismo , VirulênciaRESUMO
As a result of long-standing screening of chemopreparations of anti-rickettsiosis action in-vitro and in-vivo experiments and their clinical evaluation in the treatment of R. of any etiology, it is established that the most effective ones are those of the 4 groups: tetracyclines (doxicycline, minocyclines), ansamicines (rifampicine), ftorchinolones (cyprofloxacine, ofloxacine, pefloxacine) and the new generation of macrolides (asitromicine, klaritromicine). Since all the studied preparations have only rickettsiosis-static effect, a promising way to finding a rickettsiocidic preparation capable of preventing a possible persistence of a relevant agent in a human body is a combined application of highly effective chemopreparations (doxicycline, in particular) or pefloxacine with lysosomal alkalinizing drugs (chlorochin, ammonium chloride or amantadine). Antibiotics of prolonged anti-rickettsiosis action give a real chance for prevention even with a one-time dose.
Assuntos
Infecções por Bactérias Gram-Negativas/tratamento farmacológico , Infecções por Bactérias Gram-Negativas/prevenção & controle , Animais , Antibacterianos/uso terapêutico , Avaliação de Medicamentos , Avaliação Pré-Clínica de Medicamentos , HumanosRESUMO
The informative indices of antibiotic efficacy in the treatment of typhus were determined in experiments on guinea pigs and used for the design of an integral index of a drug prophylactic activity against Rickettsia prowazekii. A procedure for a comprehensive estimation of an antibiotic prophylactic efficacy in experimental typhus by the whole complex of clinical, immunological and rickettsiological properties is described.