Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 2 de 2
Filtrar
Mais filtros











Base de dados
Intervalo de ano de publicação
1.
J Int Med Res ; 37(3): 650-61, 2009.
Artigo em Inglês | MEDLINE | ID: mdl-19589247

RESUMO

Hepatitis B virus (HBV) infection exhibits a very narrow host range and shows a strong tropism for liver parenchymal cells, however none of the previously established experimental models can reproduce the natural process of HBV infection. In the present study, primary human hepatocytes were fused with HepG2 cells to establish the hybrid HepCHLine-4 cell line with high susceptibility to HBV. The HepCHLine-4 cells expressed HBV-specific antigen when co-incubated with HBV-positive serum from a hepatitis B patient. Post-infection, HBV relaxed circular DNA and covalently closed circular DNA were detected in HepCHLine-4 cells using a nested polymerase chain reaction, and HBV-specific particles were visualized by electron microscopy of the culture media of HepCHLine-4 cells. HepG2 cells were not susceptible to HBV infection under the same conditions. The HepCHLine-4 cells can be sub-cultured for > 12 months while maintaining susceptibility to HBV and may, therefore, be useful for studying HBV infection and the viral life cycle in human hepatocytes.


Assuntos
Técnicas de Cultura de Células/métodos , Fusão Celular/métodos , Vírus da Hepatite B/fisiologia , Hepatócitos/citologia , Hepatócitos/virologia , Linhagem Celular Tumoral , Meios de Cultura , DNA Circular/análise , DNA Viral/metabolismo , Antígenos de Superfície da Hepatite B/análise , Antígenos de Superfície da Hepatite B/ultraestrutura , Vírus da Hepatite B/ultraestrutura , Hepatócitos/ultraestrutura , Humanos , Espaço Intracelular/ultraestrutura , Espaço Intracelular/virologia , Masculino , Ultracentrifugação , Vírion/ultraestrutura
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA