RESUMO
Two plasmid vectors, which allow the recombinant polypeptides of Lassa and Marburg viruses to be expressed in prokaryotic cells E. coli strain BL21 (DE3), were produced. The two recombinant polypeptides are able to bind specific antibodies. This provides an opportunity to use them as antigenic components of immunoassay diagnostic test kits.
Assuntos
Vírus Lassa , Marburgvirus , Proteínas do Nucleocapsídeo , Proteínas Recombinantes , Animais , Reações Antígeno-Anticorpo , Escherichia coli , Humanos , Febre Lassa/imunologia , Vírus Lassa/imunologia , Vírus Lassa/isolamento & purificação , Doença do Vírus de Marburg/imunologia , Marburgvirus/imunologia , Marburgvirus/isolamento & purificação , Proteínas do Nucleocapsídeo/genética , Proteínas do Nucleocapsídeo/imunologia , Proteínas do Nucleocapsídeo/isolamento & purificação , Proteínas Recombinantes/genética , Proteínas Recombinantes/imunologia , Soro/imunologiaRESUMO
The results of the analysis of hepatitis A morbidity during the period of 1996 - 2001, are presented. The cyclic character of this morbidity in its dynamics over the period of several years was noted with the maximum morbidity level reached in 2000. The monthly dynamics of hepatitis A morbidity reflected its seasonal character with the maximum increase in autumn and winter. The virological control of drinking water revealed its contamination in spring and summer, with no subsequent rise in morbidity. The control of sewage reflected the emergence of the virus in the city collector in accordance with the increased morbidity.
Assuntos
Hepatite A/epidemiologia , Inquéritos Epidemiológicos , Humanos , Morbidade , República de Belarus/epidemiologia , Fatores de Risco , Estações do Ano , Esgotos/microbiologia , População UrbanaRESUMO
A total of 164 patients with schizophrenia and exogenic-organic psychoses resistant to psychotropic therapy were subjected to clinico-immunological examination. The inclusion into the multiple-modality therapy of the immunomodulator levamisole, besides the general improvement, had a positive effect on the psychopathologic symptomatology and helped to reduce a number of productive syndromes, deficit abnormalities and attendant extrapyramidal manifestations. Changes in immunological parameters showed a favourable course; it was expressed in depressed levels of neurosensitization, normalization of the concentrations of serum immunoglobulins, share and absolute amounts of T- and B-lymphocytes and elevation of their functional activity. The corrective effect of levamisole on humoral immunity seems to be a significant factor of overcoming tolerance to psychopharmacotherapy. The conducted study makes it possible to recommend the use of levamisole in the treatment of resistant forms of schizophrenia running an unfavourable course, exogenic-organic and other psychoses.
Assuntos
Adjuvantes Imunológicos , Levamisol/uso terapêutico , Transtornos Neurocognitivos/tratamento farmacológico , Esquizofrenia/tratamento farmacológico , Adulto , Idoso , Formação de Anticorpos/efeitos dos fármacos , Doença Crônica , Avaliação de Medicamentos , Resistência a Medicamentos , Quimioterapia Combinada , Feminino , Humanos , Imunidade Celular/efeitos dos fármacos , Masculino , Pessoa de Meia-Idade , Transtornos Neurocognitivos/imunologia , Psicotrópicos/uso terapêutico , Esquizofrenia/imunologiaAssuntos
Imunoglobulinas/metabolismo , Deficiência Intelectual/metabolismo , Criança , Disgamaglobulinemia , Feminino , Humanos , Deficiência de IgA , Deficiência de IgG , Imunoglobulina A/metabolismo , Imunoglobulina G/metabolismo , Imunoglobulina M/deficiência , Imunoglobulina M/metabolismo , Imunoglobulinas/deficiência , Deficiência Intelectual/sangue , MasculinoRESUMO
A clinico-immunological examination involved 39 patients with a prolonged unfavourable course of schizophrenia at the stage of disease exacerbation. Cytotoxic cerebral antibodies determined in the subcomissural organ-glomerular zone of the adrenal cortex model were found in 94.8% of those studied which was significantly higher than the amount of complement binding antibodies (59.0%). The cytotoxic action of sera was paralleled by the neurosensitization of T lymphocytes in the presence of their functional activity decrease. Many seropositive patients exhibited an increase in one, two or three classes of immunoglobulins which appears to confirm indirectly the correlation between the above phenomena. A comprehensive clinico-immunological examination of schizophrenic patients should facilitate a more profound insight into the pathogenesis of the disease and the selection of the appropriate therapy.
Assuntos
Encéfalo/imunologia , Citotoxicidade Imunológica , Esquizofrenia/imunologia , Adulto , Anticorpos/análise , Doença Crônica , Testes Imunológicos de Citotoxicidade , Feminino , Humanos , Imunoglobulina A/análise , Imunoglobulina G/análise , Imunoglobulina M/análise , Ativação Linfocitária , Masculino , Pessoa de Meia-Idade , Esquizofrenia Catatônica/imunologia , Esquizofrenia Paranoide/imunologia , Linfócitos T/imunologiaRESUMO
The study of immunoglobulin levels and neuroimmune processes in younger schoolchildren with slightly retarded intellectual development revealed the deficiency of immunoglobulin A (either alone or in combination with other types of immunoglobulins). The neuroimmune processes identified were characterized by the presence of brain antibodies predominantly against interspecific antigens. During psychostimulant and general somatic therapy, the concentration of immunoglobulins tended towards normal and the intensity of neuroimmune processes declined.
Assuntos
Autoanticorpos/análise , Encéfalo/imunologia , Imunoglobulinas/análise , Deficiência Intelectual/imunologia , Criança , Feminino , Humanos , Imunoglobulina A/análise , Imunoglobulina G/análise , Imunoglobulina M/análise , Deficiência Intelectual/etiologia , Deficiência Intelectual/terapia , Masculino , Ácido gama-Aminobutírico/uso terapêuticoAssuntos
Autoanticorpos , Doenças do Sistema Nervoso/imunologia , Transtornos Psicóticos/imunologia , Animais , Encéfalo/imunologia , Epilepsia/imunologia , Feminino , Humanos , Troca Materno-Fetal , Transtornos Mentais/etiologia , Malformações do Sistema Nervoso , Periodicidade , Gravidez , Transtornos Puerperais/imunologia , Ratos , Esquizofrenia/imunologia , Estresse Psicológico/imunologia , SíndromeAssuntos
Transtornos Puerperais/diagnóstico , Esquizofrenia/diagnóstico , Adulto , Transtorno Bipolar/diagnóstico , Delusões/diagnóstico , Diagnóstico Diferencial , Feminino , Seguimentos , Humanos , Transtornos Mentais/genética , Gravidez , Infecção Puerperal/diagnóstico , Recidiva , Esquizofrenia Catatônica/diagnóstico , Psicologia do Esquizofrênico , Transtorno da Personalidade Esquizotípica/diagnóstico , Ajustamento SocialRESUMO
On the basis of comprehensive immuno-biological studies of schizophrenia where the disease manifests itself after delivery, the authors come to the conclusion that a certain role is played in the development of the disease by immuno-allergic processes. Facts confirming this supposition are a detection of brain antigens and the expressiveness of sensitization even during the initial stage of the disease, a sufficiently high frequency of revealed automtibodies, their correlation with the quality to remissions. Besides these facts, the authors were able to distinguish individually different types of antibody formation in recurrent and attack-like-progressive forms of schizophrenia, a fall in the complement activity in the blood serum of patients as well as a certain connection between the indicis of organopecific immunity and the dynamics of the morbid process.
Assuntos
Autoanticorpos , Encéfalo/imunologia , Transtornos Puerperais/imunologia , Esquizofrenia/imunologia , Formação de Anticorpos , Antígenos , Proteínas do Sistema Complemento , Feminino , Humanos , Neutrófilos/imunologia , Gravidez , RecidivaRESUMO
The authors report of some results of a clinical follow-up study of 76 schizophrenic patients in psychosis observed during the puerperal period. Two types of the process were distinguished: a recurrent type and attack-like progressive. Some diffrential diagnostical criteria are given permitting to differ recurrent schizophrenia from somatogenic and infectious psychoses.
Assuntos
Transtornos Puerperais/diagnóstico , Esquizofrenia/diagnóstico , Adulto , Diagnóstico Diferencial , Feminino , Humanos , Gravidez , Complicações na Gravidez , Transtornos Puerperais/complicações , Recidiva , Esquizofrenia/etiologia , Psicologia do Esquizofrênico , SíndromeRESUMO
The authors compare some clinico-psychopathological traits in the development and frequency of detected antibrain antibodies and allergical states in epilepsy with a different lateralization in the hemispheres. In a sinistral localization of the lesion there is a significant increase of an unfavourable development of the disease and a more frequent detection of antibodies to the homologous brain. In a dextral localization there is a relatively favourable development of the basic disorder. The authors discuss the influence of the sex factor on the immunobiological indices and the lateralization of the hemisphere. A theory is suggested that the left and right hemispheres are involved in different ways in the different links of immunity, and their pathology may provoke different changes in the body reactivity.