RESUMO
In the title compound, C17H21ClNO4P·C3H7NO, the dihedral angle formed by the aromatic rings is 83.98â (7)°. In the crystal, O-Hâ¯O, N-Hâ¯O and C-Hâ¯O hydrogen bonds link the mol-ecules into double layers parallel to (011).
RESUMO
In the title Cu(II) complex, [Cu(C(7)H(3)NO(4))(C(6)H(5)NO(2))(H(2)O)]·H(2)O, the environment of the Cu(2+) ion is a distorted square pyramid with the axial site occupied by the O atom from the coordinated water mol-ecule and the square base formed by two O and two N atoms from the tridentate anion and the neutral monodentate pyridine-3-carboxylic acid ligand. O-Hâ¯O hydrogen bonds, as well as π-π inter-actions [centroid-centroid distance = 3.945â (3)â Å] contribute to the stabilization of this structure.
RESUMO
DLC1 (deleted in liver cancer-1) is a new candidate tumor suppressor gene, which is inactive in various types of human cancers including colon cancer. To study the function of DLC1, we constructed a pcDNA3.1 vector containing the DLC1 gene and transfected it into HT29 colon cancer cells that were deficient in DLC1 expression. The restoration of DLC1 expression in HT29 cells significantly inhibited cell proliferation and migration. Flow cytometry showed that DLC1 transfection into HT29 cells induced apoptosis and that the cell cycle was arrested at S-phase. Additionally, cyclinD1 mRNA and protein expression were down-regulated while p21 expression was increased in pcDNA3.1-DLC1-HT29 cells compared to wild HT29 cells. These results confirm the role of DLC1 gene as a tumor suppressor, which may be manifested by regulation of p21 and cyclinDl. The DLC1 gene has a potential therapeutic role in inhibiting the development of colon cancer.
Assuntos
Movimento Celular/genética , Proliferação de Células , Proteínas Supressoras de Tumor/genética , Apoptose/genética , Ciclo Celular/genética , Ciclina D1/genética , Ciclina D1/metabolismo , Inibidor de Quinase Dependente de Ciclina p21/genética , Inibidor de Quinase Dependente de Ciclina p21/metabolismo , Proteínas Ativadoras de GTPase , Expressão Gênica/genética , Células HT29 , Humanos , Invasividade Neoplásica/genética , Transfecção , Ensaio Tumoral de Célula-Tronco , Proteínas Supressoras de Tumor/metabolismoRESUMO
The conformation of the title compound, C(7)H(8)N(2)O, is stabilized by an intra-molecular O-Hâ¯N hydrogen bond. The crystal structure shows inter-molecular N-Hâ¯O hydrogen bonds.