RESUMO
Intestinal microflora in healthy rats and its changes under the conditions of experimental chronic toxic hepatitis were studied. The study revealed that in intact animals the microflora of the small intestine was represented by bacteria of the genera Escherichia, Enterobacter, Moraxella, Alcaligenes, Staphylococcus, Streptococcus. Bacteria of the genera Escherichia, Enterobacter, Moraxella, Alcaligenes, Staphylococcus, Corynebacterium and Clostridium were isolated from the large intestine. No bacteria were found in the systemic blood, the contents of the portal vein, as well as in the liver parenchyma and the mesenterial lymph nodes. As the result of dysbiosis induced by the introduction of kanamycin and in chronic hepatitis caused by carbon tetrachloride the sharp decrease in the species composition of microbial communities (up to 2-3 species) in the small intestine and was observed along with penetration of bacteria into the blood stream, the mesenterial lymph nodes and the liver parenchyma. The tendency towards the restoration of the quantitative and qualitative microflora composition was noted following administration into experimental animals of bactisubtil and amixin--an inductor of interferonogenesis.
Assuntos
Doença Hepática Crônica Induzida por Substâncias e Drogas/microbiologia , Intestino Delgado/microbiologia , Adjuvantes Imunológicos/uso terapêutico , Animais , Antibacterianos/uso terapêutico , Bacillus subtilis/isolamento & purificação , Bacteriemia , Translocação Bacteriana/efeitos dos fármacos , Fatores Biológicos/uso terapêutico , Tetracloreto de Carbono , Doença Hepática Crônica Induzida por Substâncias e Drogas/sangue , Bactérias Gram-Positivas/isolamento & purificação , Bactérias Gram-Positivas/fisiologia , Indutores de Interferon/uso terapêutico , Canamicina , Fígado/microbiologia , Linfonodos/microbiologia , Masculino , Proteobactérias/isolamento & purificação , Ratos , Ratos Wistar , Tilorona/uso terapêuticoRESUMO
The functional activity of alveolar macrophages obtained from mice, both healthy and infected with influenza virus A/Aichi 2/68 (H3N2), as manifested by their capacity to initiate the development of primary immune response to sheep red blood cells and Escherichia coli lipopolysaccharide after the transfer of these macrophages to intact syngeneic recipients was studied. The capacity of alveolar macrophages to perform antigen-presenting functions in the induction of humoral immune response was shown, and at the same time the development of experimental influenza infection was found to essentially decrease these properties. The injection of the immunomodulating agent diuciphon into experimental mice somewhat enhanced the immune response after the syngeneic transfer of alveolar macrophages from infected mice to intact recipients.