[Modified method of isolating thymopoietin and its effect on neuromuscular transmission]. / Modifikatsiia metoda vydeleniia timopoétina i ego vliianie na nervno-myshechnuiu peredachu.

A modified method for isolation of the calf thymus hormone thymopoetin has been developed. For hormone purification high-speed multi-stage centrifugation and gel-filtration on sefadexes were used instead of filtration. The thymus hormonal factor was identified on the basis of its ability to block neuromuscular transmission.

[Stimulation and suppression of contact dermatitis in mice with a low-molecular thymic humoral factor]. / Stimuliatsiia i supressiia kontaktnogo dermatita u myshei nizkomolekuliarnym gumoral'nym faktorom timusa.

The authors studied the influence of the low molecular lymphocyte-stimulating substance (LSS) of the thymus on the development of contact sensitivity to picrylchloride in mice. As revealed, low LSS doses enhanced, and high doses suppressed this type of delayed hypersensitivity. Contact sensitivity was passively transfered by means of the lymph node and spleen cells isolated on the 6th day after the immunization. Experiments showed mice given high LSS doses to contain cells suppressing passive transfer of contact sensitivity by immune cells. This suppression was annulled after the treatment of cells with theta-antiserum and the complement. A conclusion was drawn that suppressor cells influenced the effector phase of contact sensitivity.

[Influence of thymosin on some indices of immunogenesis in intact and adrenalectomized guinea pigs]. / Vliianie timozina na nekotorye pokazateli immunogeneza u neoperirovannykh i adrenaléktomirovannykh morskikh svinok

The authors studied the effect of injections of the biologically-active preparation of the thymus -- thymosine -- on the development of delayed hypersensitivity (DHS) and hypersensitivity of the immediate type, as well as on the number of rosette-forming and antibody-forming cells in adrenalectomized and unoperated guinea pigs. Thymosine produced more intensive influence upon the immunological status of the organism in adrenalectomized animals. Under conditions of sensitization used by the authors adrenalectomy enhanced the DHS manifestations in this work at the initial stage of its development; this intensification was of short duration. However, thymosine injections promoted a more prolonged and intensive expression of these sequelae of adrenalectomy. At some of the stages, there was also an increase in the number of antibody-forming and rosette-forming cells under the effect of thymosine. Thymosine gave a more pronounced effect when administered during the sensitization. The periods of supervention of the immediate type of reaction depended on the scheme of thymosine administration.