HR-LCMS-Based Metabolite Profiling, Antioxidant, and Anticancer Properties of Teucrium polium L. Methanolic Extract: Computational and In Vitro Study.

In this study, we investigate the phytochemical profile, anticancer, and antioxidant activities of Teucrium polium methanolic extract using both in vitro and in silico approaches. The results showed the identification of 29 phytochemical compounds belonging to 13 classes of compounds and 20 tripeptides using High Resolution-Liquid Chromatography Mass Spectrometry (HR-LCMS). 13R-hydroxy-9E,11Z octadecadienoic acid, dihydrosamidin, valtratum, and cepharantine were the main compounds identified. The tested extract showed promising antioxidant activities (ABTS-IC50 = 0.042 mg/mL; 1,1-diphenyl-2-picrylhydrazyl (DPPH)-IC50 = 0.087 mg/mL, ß-carotene-IC50 = 0.101 mg/mL and FRAP-IC50 = 0.292 mg/mL). Using both malignant Walker 256/B and MatLyLu cell lines, T. polium methanolic extract showed a dose/time-dependent antitumor activity. The molecular docking approach revealed that most of the identified molecules were specifically binding with human peroxiredoxin 5, human androgen, and human progesterone receptors with high binding affinity scores. The obtained results confirmed that T. polium is a rich source of bioactive molecules with antioxidant and antitumor potential.

Description of microbial diversity associated with ticks Hyalomma dromedarii (Acari: Ixodidae) isolated from camels in Hail region (Saudi Arabia) using massive sequencing of 16S rDNA.

Ticks are blood feeder able to transmit a wide diversity of microbes including pathogens. Therefore, it is of our interest to detect the diversity of microorganisms residing within ticks using massive sequencing of 16S rDNA. In this study, 200 adult ticks were collected from healthy camels in two localities from Hail province (Saudi Arabia). The analysis showed high microbial diversity dominated by the two domains (Archaea and Bacteria) associated with Hyalomma dromedarii from both regions. Proteobacteria (61.3%) and Firmicutes (31.2%) dominated the ticks from the Al Khotha region. While, the microbiome of ticks from the Al Gayed region was dominated by Proteobacteria (81.2%) and Firmicutes (9.2%). Twenty-three families were identified in the DNA-pool from the Al Gayed region, and was dominated by Pseudomonadaceae (45.37%), and Marinobacteraceae (14.39%) families. Francisellaceae (46%), Staphylococcaceae (24.26%) dominated the microbiome of the ticks collected from Al Gayed region. Thus, the genera Pseudomonas, Francisella, Proteus, Marinobacter, Glutamicibacter, Pedobacter, and Staphylococcus are largely distributed in the two identified microbiomes. This study concluded that ticks collected from the studied localities contained a wide range of microbial communities. These data have a great veterinary and medical importance in near future.

Phytochemical Screening, Antibacterial, Antifungal, Antiviral, Cytotoxic, and Anti-Quorum-Sensing Properties of Teucrium polium L. Aerial Parts Methanolic Extract.

The chemical profile of Teucrium polium L. (T. polium) methanolic extract was tested using liquid chromatography coupled with high resolution mass spectrometry (HR-LCMS). Disc diffusion and microdilution assays were used for the antimicrobial activities. Coxsackievirus B-3 (CVB3) and Herpes simplex virus type 2 (HSV-2) were used for the antiviral activities. Chromobacterium violaceum (ATCC 12472 and CV026) and Pseudomonas aeruginosa PAO1 were used as starter strains for the anti-quorum sensing tests. Isoprenoids are the main class of compounds identified, and 13R-hydroxy-9E,11Z-octadecadienoic acid, valtratum, rhoifolin, sericetin diacetate, and dihydrosamidin were the dominant phytoconstituents. The highest mean diameter of growth inhibition zone was recorded for Acinetobacter baumannii (19.33 ± 1.15 mm). The minimal inhibitory concentrations were ranging from 6.25 to 25 mg/mL for bacterial strains, and from 6.25 to 25 mg/mL for Candida species. The 50% cytotoxic concentration on VERO (African Green Monkey Kidney) cell lines was estimated at 209 µg/mL. No antiviral activity was recorded. Additionally, T. polium extract was able to inhibit P. aeruginosa PAO1 motility in a concentration-dependent manner. However, the tested extract was able to inhibit 23.66% of the swarming and 35.25% of swimming capacities of PAO1 at 100 µg/mL. These results highlighted the role of germander as a potent antimicrobial agent that can interfere with the virulence factors controlled by the quorum-sensing systems.

Nano-cellulose based nano-coating biomaterial dataset using corn leaf biomass: An innovative biodegradable plant biomaterial.

The nanocellulose derived biodegradable plant biomaterial as nano-coating can be used in the medical, biomedical cosmetics, and bioengineering products. Bio-plastic and some synthetic derived materials are edible and naturally biodegradable. The study was conducted to investigate edible nano-biopolymer based nano-coating of capsules and drugs or other definite biomedical materials from corn leaf biomass. Corn leaf biomass was used as an innovative sample to produce edible nano-coating bioplastic for drug and capsule coating and other industrial uses. The data show the negligible water 0.01% absorbed by bio-plastic nanocoating. Odor represented by burning test was under the completely standard based on ASTM. Moreover, data on color coating, tensile strength, pH, cellulose content have been shown under standard value of ASTM (American standard for testing and materials) standard. In addition to that data on the chemical element test like K+, CO3-- , Cl-, Na+ exhibited positive data compared to the synthetic plastic in the laboratory using the EN (166)) standardization. Therefore, it can be concluded that both organic (cellulose and starch) based edible nano-coating bioplastic may be used for drug and capsule coating as biomedical and medical components in the pharmaceutical industries.

Nano-cellulose biopolymer based nano-biofilm biomaterial using plant biomass: An innovative plant biomaterial dataset.

The nano-cellulose derived nano-biofilm keeps a magnificent role in medical, biomedical, bioengineering and pharmaceutical industries. Plant biomaterial is naturally organic and biodegradable. This study has been highlighted as one of the strategy introducing biomass based nano-bioplastic (nanobiofilm) to solve dependency on petroleum and environment pollution because of non-degradable plastic. The data study was carried out to investigate the nano-biopolymer (nanocellulose) based nano-biofilm data from corn leaf biomass coming after bioprocess technology without chemicals. Corn leaf biomass was used to produce biodegradable nano-bioplastic for medical and biomedical and other industrial uses. Data on water absorption, odor, pH, cellulose content, shape and firmness, color coating and tensile strength test have been exhibited under standardization of ASTM (American standard for testing and materials). Moreover, the chemical elements of nanobiofilm like K+, CO3--, Cl-, Na+ showed standard data using the EN (166).