Human T cells show plasticity for direct recognition of xenogeneic dendritic cells.

Organ shortage continues to be the forefront of problems facing clinical transplantation. Although xenografts serve as a promising alternative, its success is contingent upon further investigation into the mechanisms of cell-mediated xenograft rejection. Here, we explored the direct and indirect contribution of human immune cells in xenorecognition using human and murine in vitro coculture systems. Our data shows that human T cells directly recognized the xenogeneic MHC molecules since blocking of MHCs suppressed their proliferative response and cytokines production of IL-2 and IFN-γ. While B and NK cells alone did not generate a significant response, the combination of B and T cells promoted indirect xenorecognition by T cells as evidenced by an increase in B cell proliferative response. Overall, our data suggests that human T cells have the plasticity to recognize xenogeneic MHCs and contribute to xenograft rejection.

Quantitative PCR marker genes for endometrial adenocarcinoma.

Endometrial adenocarcinoma is a common malignancy in women worldwide, with formation of remote metastasis occurring following oncological treatment. Circulating tumor cells (CTCs) are regarded to be the origin of haematogenous metastasis formation. The present study aimed to identify suitable marker genes using a quantitative polymerase chain reaction (qPCR) approach to detect CTCs from blood samples of patients with endometrial carcinoma. Therefore, RNA was isolated from endometrial adenocarcinoma cell lines and from healthy endometrial tissue and reverse transcribed to cDNA, which was then used in qPCR on a number of marker genes. Cytokeratin 19 and claudin 4 were identified as suitable marker genes for CTCs in endometrial adenocarcinoma, due to their high expression in the majority of the cell lines investigated. The expression values of the genes examined varied widely between the different cell lines, which is similar to the variation in the patient samples. Therefore, the necessity for a set of genes for CTC detection and not one single marker gene is demonstrated. qPCR is a fast, cost­efficient and easy to perform technique, which may be used in the detection of CTCs. Investigation of the occurrence of CTCs in cancer patients would aid in the prevention of metastasis and thereby refine treatment.

Trustworthiness as a clinical variable: the problem of trust in the management of chronic, nonmalignant pain.

The subjective nature of pain leads to many treatment difficulties. These problems can often be resolved if we know that the patient is trustworthy. Trustworthiness should be assessed as a distinct clinical variable. This is more easily achieved if we examine the three components of trustworthiness: the patient's subjective reports, which we call testimony; the reason that the patient seeks treatment, which we call motive; and the patient's adherence with efforts to get well, which we call responsibility. Because of difficulties with assessing testimony and motive, we propose that establishing the patient's responsibility is the key to assessing trustworthiness.

Psychosocial therapies for neck pain.

The biopsychosocial approach provides the necessary framework for understanding and treating chronic pain. Through education, cognitive-behavioral therapy, relaxation training, and active adaptation, the biopsychosocial approach allows patients to learn to control their internal environments (pain-related thoughts and emotions) and to influence their responses to the external environment (physical condition, work, significant others, and other stresses). This education-based model of therapy combines naturally with the medical model and medical care.