Trans-Cinnamaldehyde Inhibition of Pyruvate Dehydrogenase: Effects on Streptococcus mutans Carbohydrate Metabolism.

Dental caries is a chronic oral infectious disease, and Streptococcus mutans (S. mutans) plays an important role in the formation of dental caries. Trans-cinnamaldehyde (CA) exhibits broad-spectrum antibacterial activity; however, its target and mechanism of action of CA on S. mutans needs to be further explored. In this study, it was verified that CA could inhibit the growth and biofilm formation of S. mutans. Further proteomic analysis identified 33, 55, and 78 differentially expressed proteins (DEPs) in S. mutans treated with CA for 1, 2, and 4 h, respectively. Bioinformatics analysis showed that CA interfered with carbohydrate metabolism, glycolysis, pyruvate metabolism, and the TCA cycle, as well as amino acid metabolism of S. mutans. Protein interactions suggested that pyruvate dehydrogenase (PDH) plays an important role in the antibacterial effect of CA. Moreover, the upstream and downstream pathways related to PDH were verified by various assays, and the results proved that CA not only suppressed the glucose and sucrose consumption and inhibited glucosyltransferase (GTF) and lactate dehydrogenase (LDH) activities but also decreased the ATP production. Interestingly, the protein interaction, qRT-PCR, and molecular docking analysis showed that PDH might be the target of CA to fight S. mutans. In summary, the study shows that CA interferes with the carbohydrate metabolism of bacteria by inhibiting glycolysis and the tricarboxylic acid (TCA) cycle via binding to PDH, which verifies that PDH is a potential target for the development of new drugs against S. mutans.

Hydroxypropyl chitosan/ε-poly-l-lysine based injectable and self-healing hydrogels with antimicrobial and hemostatic activity for wound repair.

The biggest obstacle to treating wound healing continues to be the production of simple, inexpensive wound dressings that satisfy the demands associated with full process of repair at the same time. Herein, a series of injectable composite hydrogels were successfully prepared by a one-pot method by utilizing the Schiff base reaction as well as hydrogen bonding forces between hydroxypropyl chitosan (HCS), ε-poly-l-lysine (EPL), and 2,3,4-trihydroxybenzaldehyde (TBA), and multiple cross-links formed by the reversible coordination between iron (III) and pyrogallol moieties. Notably, hydrogel exhibits excellent physicochemical properties, including injectability, self-healing, water retention, and adhesion, which enable to fill irregular wounds for a long period, providing a suitable moist environment for wound healing. Interestingly, the excellent hemostatic properties of the hydrogel can quickly stop bleeding and avoid the serious sequelae of massive blood loss in acute trauma. Moreover, the powerful antimicrobial and antioxidant properties also protect against bacterial infections and reduce inflammation at the wound site, thus promoting healing at all stages of the wound. The study of biohydrogel with multifunctional integration of wound treatment and smart medical treatment is clarified by this line of research.

Random Silica-Glass Microlens Arrays Based on the Molding Technology of Photocurable Nanocomposites.

Random microlens arrays (rMLAs) have been widely applied as a beam-shaping component within an optical system. Silica glass is undoubtedly the best choice for rMLAs because of its wide range of spectra with high transmission and high damage threshold. Yet, machining silica glass with user-defined shapes is still challenging. In this work, novel design and fabrication methods of random silica-glass microlens arrays (rSMLAs) are proposed and a detailed investigation of this technology is presented. Based on the molding technology, the fabricated rSMLAs with tunable divergent angles demonstrate superior physical properties with 1.81 nm roughness, 1074.33 HV hardness, and excellent thermal stability at 1250 °C for 3 h. Meanwhile, their characterized optical performance shows a high transmission of over 90% in the ultraviolet spectrum. The fabricated two types of rSMLAs exhibit excellent effects of beam homogenization with surprising energy utilization (more than 90%) and light spot uniformity (more than 80%). This innovative process paves a new route for fabricating rMLAs on solid silica glass and breaking down the barrier of rMLAs to broader applications.

Sulfadiazine removal by peroxymonosulfate activation with sulfide-modified microscale zero-valent iron: Major radicals, the role of sulfur species, and particle size effect.

Sulfide-modified zero-valent iron (S-Fe0) is regarded as a promising method to enhance the catalytic activity of Fe0 for peroxymonosulfate (PMS) activation. However, the roles of sulfidation and the application of the sulfidation treatment method are worth to further investigation. In our study, the effects of the S/Fe ratio, Fe0 dosage, and initial pH on sulfadiazine (SDZ) removal were investigated. The characterization of S-Fe0 with SEM, XPS, contact angle and Tafel analysis confirmed that the formation of sulfur species on the Fe0 surface could enhance the catalytic performance of Fe0. S2- played the major role and SO32- played the minor role in accelerating the conversion of Fe3+ to Fe2+. EPR tests, radical quenching and quantitative determination experiments identified •OH as playing the major role and SO4•- also playing an important role in SDZ removal in S-Fe0/PMS system. Sulfidation produced no notable change in the role of •OH and SO4•-. A possible degradation pathway of SDZ was proposed. Effect of sulfidation on various sizes of Fe0 was also studied which demonstrated that the smaller sizes of Fe0 (< 8 µm) were more effective in the sulfidation method treatment. S-Fe0/PMS system also showed a good performance in removing antibiotics in natural fresh water.