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Responsive luminescent materials that reversibly react to external stimuli have emerged as prospective platforms for information encryption applications. Despite brilliant achievements, the existing fluorescent materials usually have low information density and experience inevitable information loss when subjected to mechanical damage. Here, inspired by the hierarchical nanostructure of fluorescent proteins in jellyfish, we propose a self-healable, photoresponsive luminescent elastomer based on dynamic interface-anchored borate nanoassemblies for smart dual-model encryption. The rigid cyclodextrin molecule restricts the movement of the guest fluorescent molecules, enabling long room-temperature phosphorescence (0.37 s) and excitation wavelength-responsive fluorescence. The building of reversible interfacial bonding between nanoassemblies and polymer matrix together with their nanoconfinement effect endows the nanocomposites with excellent mechanical performances (tensile strength of 15.8 MPa) and superior mechanical and functional recovery capacities after damage. Such supramolecular nanoassemblies with dynamic nanoconfinement and interfaces enable simultaneous material functionalization and self-healing, paving the way for the development of advanced functional materials.
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The early identification of rotten potatoes is one of the most important challenges in a storage facility because of the inconspicuous symptoms of rot, the high density of storage, and environmental factors (such as temperature, humidity, and ambient gases). An electronic nose system based on an ensemble convolutional neural network (ECNN, a powerful feature extraction method) was developed to detect potatoes with different degrees of rot. Three types of potatoes were detected: normal samples, slightly rotten samples, and totally rotten samples. A feature discretization method was proposed to optimize the impact of ambient gases on electronic nose signals by eliminating redundant information from the features. The ECNN based on original features presented good results for the prediction of rotten potatoes in both laboratory and storage environments, and the accuracy of the prediction results was 94.70% and 90.76%, respectively. Moreover, the application of the feature discretization method significantly improved the prediction results, and the accuracy of prediction results improved by 1.59% and 3.73%, respectively. Above all, the electronic nose system performed well in the identification of three types of potatoes by using the ECNN, and the proposed feature discretization method was helpful in reducing the interference of ambient gases.
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Potatoes are popular among consumers due to their high yield and delicious taste. However, due to the numerous varieties of potatoes, different varieties are suitable for different processing methods. Therefore, it is necessary to distinguish varieties after harvest to meet the needs of processing enterprises and consumers. In this study, a new visible-near-infrared spectroscopic analysis method was proposed, which can achieve detection of five potato varieties. The method measures the transmission and reflection spectra of potatoes using a spectral acquisition system, encodes one-dimensional spectra into two-dimensional images using Gramian Angular Summation Field (GASF), Gramian Angular Difference Field (GADF), Markov Transition Field (MTF) and Recurrence Plot (RP), and improves the coordinated attention mechanism module and embeds the improved module into the ConvNeXt V2 model to build the ConvNeXt V2-CAP model for potato variety classification. The results show that compared with directly using one-dimensional classification models, image encoding of spectral data for classification greatly improves the accuracy. Among them, the best accuracy of 99.54% is achieved by using GADF image encoding of transmission spectra combined with the ConvNeXt V2-CAP model for classification, which is 16.28% higher than the highest accuracy of the one-dimensional classification model. The CAP attention mechanism module improves the performance of the model, especially when the dataset is small. When the training set is reduced to 150 images, the accuracy of the model is improved by 2.33% compared to the original model. Therefore, it is feasible to classify potato varieties using visible-near infrared spectroscopy and image encoding technology.
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Fermented foods are sensitive to the production conditions because of microbial and enzymatic activities, which requires intelligent flavor sensing system (IFSS) to monitor and optimize the production process based on the flavor properties. As the simulation system of human olfaction and gustation, IFSS has been widely used in the field of food with the characteristics of nondestructive, pollution-free, and real-time detection. This paper reviews the application of IFSS in the control of fermentation, ripening, and shelf life, and the potential in the identification of quality differences and flavor-producing microbes in fermented foods. The survey found that electronic nose (tongue) is suitable to monitor fermentation process and identify food authenticity in real time based on the changes of flavor profile. Gas chromatography-ion mobility spectrometry and nuclear magnetic resonance technology can be used to analyze the flavor metabolism of fermented foods at various production stages and explore the correlation between flavor substances and microorganisms.
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In this study, three modified glassy carbon electrodes based on three-dimensional conducting polymer nanocomposites (TDCPNs) were fabricated for evaluating the aging process of Huangjiu (Chinese rice wines). The electrochemical activity and experimental conditions of the TDCPNs modified electrodes were investigated by cyclic voltammetry, the aging information obtained by the modified electrodes were optimized by variance inflation factor (VIF). Principal components analysis (PCA), locally linear embedding (LLE), and locality preserving projection (LPP, which presented the best classification result) based on the optimized data were applied to classify the wine samples. Then, the dimensionality reduction data of PCA, LLE, and LPP were used as input variables of the logistic regression and extreme learning machine (ELM) for evaluating the aging process of Huangjiu, and the LLE-ELM method exhibited the best prediction results. These results demonstrated that the TDCPNs modified electrodes presented the potential for the quality analysis of food and beverages.
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Nanocompostos , Vinho , China , Técnicas Eletroquímicas , Eletrodos , Análise de Componente Principal , Vinho/análiseRESUMO
Aroma and taste are the most important attributes of alcoholic beverages. In the study, the self-developed electronic tongue (e-tongue) and electronic nose (e-nose) were used for evaluating the marked ages of rice wines. Six types of feature data sets (e-tongue data set, e-nose data set, direct-fusion data set, weighted-fusion data set, optimized direct-fusion data set, and optimized weighted-fusion data set) were used for identifying rice wines with different wine ages. Pearson coefficient analysis and variance inflation factor (VIF) analysis were used to optimize the fusion matrixes by removing the multicollinear information. Two types of discrimination methods (principal component analysis (PCA) and locality preserving projections (LPP)) were used for classifying rice wines, and LPP performed better than PCA in the discrimination work. The best result was obtained by LPP based on the weighted-fusion data set, and all the samples could be classified clearly in the LPP plot. Therefore, the weighted-fusion data were used as independent variables of partial least squares regression, extreme learning machine, and support vector machines (LIBSVM) for evaluating wine ages, respectively. All the methods performed well with good prediction results, and LIBSVM presented the best correlation coefficient (R2 ≥ 0.9998).
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Nariz Eletrônico , Oryza/química , Vinho/análise , Análise Discriminante , Ouro/química , Análise dos Mínimos Quadrados , Nanopartículas Metálicas/química , Nanopartículas Metálicas/ultraestrutura , Análise de Componente Principal , Máquina de Vetores de Suporte , Paladar , Fatores de TempoRESUMO
In the study, the voltammetric electronic tongue based on three nanocomposites modified electrodes was applied for the identification of rice wines of different geographical origins. The nanocomposites were prepared by gold and copper nanoparticles in the presence of conducting polymers (polymer sulfanilic acid, polymer glutamic acid) and carboxylic multi - walled carbon nanotubes. The modified electrodes showed high sensitivity to guanosine - 5' - monophosphate disodium salt, tyrosine and gallic acid which have good correlation with the geographical origins of rice wines. Scanning electron microscopy was performed to display the surface morphologies of the nanocomposites, and cyclic voltammetry was applied to study the electrochemical behaviors of the taste substances on the electrode surfaces. Four types of electrochemical parameters (pH, scan rates, accumulation potentials and time) were optimized for getting a low limit of the detection of each taste substance. The geographical information of rice wines was obtained by the modified electrodes based on two types of multi - frequency large amplitude pulse voltammetry, and "area method" was applied for extracting the feature data from the original information obtained. Based on the area feature data, principal component analysis, locality preserving projection (LPP), and linear discriminant analysis were applied for the classification of the rice wines of different geographical origins, and LPP presented the best results; extreme learning machine (ELM) and alibrary for support vector machines were applied for predicting the geographical origins of rice wines, and ELM performed better.
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Técnicas Eletroquímicas , Nariz Eletrônico , Geografia , Nanocompostos/química , Oryza/química , Vinho/análise , EletrodosRESUMO
In this paper, poly(acid chrome blue K) (PACBK)/AuNP/glassy carbon electrode (GCE), polysulfanilic acid (PABSA)/AuNP/GCE and polyglutamic acid (PGA)/CuNP/GCE were self-fabricated for the identification of rice wines of different brands. The physical and chemical characterization of the modified electrodes were obtained using scanning electron microscopy and cyclic voltammetry, respectively. The rice wine samples were detected by the modified electrodes based on multi-frequency large amplitude pulse voltammetry. Chronoamperometry was applied to record the response values, and the feature data correlating with wine brands were extracted from the original responses using the 'area method'. Principal component analysis, locality preserving projections and linear discriminant analysis were applied for the classification of different wines, and all three methods presented similarly good results. Extreme learning machine (ELM), the library for support vector machines (LIB-SVM) and the backpropagation neural network (BPNN) were applied for predicting wine brands, and BPNN worked best for prediction based on the testing dataset (R 2 = 0.9737 and MSE = 0.2673). The fabricated modified electrodes can therefore be applied to identify rice wines of different brands with pattern recognition methods, and the application also showed potential for the detection aspects of food quality analysis.
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In this study, the changes in the quality of unshelled peanuts and peanut kernels during storage were analyzed using an electronic nose (e-nose). The physicochemical indexes (acid and peroxide values) of peanut kernels were tested by traditional method as a reference. The storage time of peanut kernels increases from left to right in the cluster analysis plot based on the physicochemical indexes. The "maximum values", "area values", and "70th s values" methods were applied to extract the feature data from the e-nose responses. Principal component analysis (PCA) results indicated that the "70th s values" method produced the most accurate results, furthermore, unshelled peanut and peanut kernel samples presented similar characteristics in the PCA plots; the partial least squares regression (PLSR) results showed that the features of unshelled peanuts and peanut kernels are highly correlated with acid and peroxide values, respectively.
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Arachis/química , Armazenamento de Alimentos , Nozes/química , Arachis/normas , Nariz Eletrônico , Qualidade dos Alimentos , Nozes/normas , Análise de Componente Principal/métodosRESUMO
Staphylococcus aureus is an opportunistic bacterial pathogen responsible for a diverse spectrum of human diseases and a leading cause of nosocomial and community-acquired infections. Development of a vaccine against this pathogen is an important goal. The fibronectin binding protein A (FnBPA) of S. aureus is one of multifunctional 'microbial surface components recognizing adhesive matrix molecules' (MSCRAMMs). It is one of the most important adhesin molecules involved in the initial adhesion steps of S. aureus infection. It has been studied as potential vaccine candidates. However, FnBPA is a high-molecular-weight protein of 106 kDa and difficulties in achieving its high-level expression in vitro limit its vaccine application in S. aureus infection diseases control. Therefore, mapping the immunodominant regions of FnBPA is important for developing polyvalent subunit fusion vaccines against S. aureus infections. In the present study, we cloned and expressed the N-terminal and C-terminal of FnBPA. We evaluated the immunogenicity of the two sections of FnBPA and the protective efficacy of the two truncated fragments vaccines in a murine model of systemic S. aureus infection. The results showed recombinant truncated fragment F130-500 had a strong immunogenicity property and survival rates significantly increased in the group of mice immunized with F130-500 than the control group. We futher identified the immunodominant regions of FnBPA. The mouse antisera reactions suggest that the region covering residues 110 to 263 (F1B110-263) is highly immunogenic and is the immunodominant regions of FnBPA. Moreover, vaccination with F1B110-263 can generate partial protection against lethal challenge with two different S. aureus strains and reduced bacterial burdens against non-lethal challenge as well as that immunization with F130-500. This information will be important for further developing anti- S. aureus polyvalent subunit fusion vaccines.
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Adesinas Bacterianas/imunologia , Mapeamento de Epitopos , Epitopos Imunodominantes/imunologia , Staphylococcus aureus/imunologia , Adesinas Bacterianas/química , Adesinas Bacterianas/genética , Animais , Vacinas Bacterianas/química , Vacinas Bacterianas/genética , Vacinas Bacterianas/imunologia , Clonagem Molecular , Soros Imunes/imunologia , Imunização , Epitopos Imunodominantes/química , Staphylococcus aureus Resistente à Meticilina/imunologia , Staphylococcus aureus Resistente à Meticilina/fisiologia , Camundongos , Fragmentos de Peptídeos/genética , Fragmentos de Peptídeos/imunologia , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/imunologia , Infecções Estafilocócicas/prevenção & controleRESUMO
A voltammetric electronic tongue (VE-tongue) was developed to discriminate the difference between Chinese rice wines in this research. Three types of Chinese rice wine with different marked ages (1, 3, and 5 years) were classified by the VE-tongue by principal component analysis (PCA) and cluster analysis (CA). The VE-tongue consisted of six working electrodes (gold, silver, platinum, palladium, tungsten, and titanium) in a standard three-electrode configuration. The multi-frequency large amplitude pulse voltammetry (MLAPV), which consisted of four segments of 1 Hz, 10 Hz, 100 Hz, and 1000 Hz, was applied as the potential waveform. The three types of Chinese rice wine could be classified accurately by PCA and CA, and some interesting regularity is shown in the score plots with the help of PCA. Two regression models, partial least squares (PLS) and back-error propagation-artificial neural network (BP-ANN), were used for wine age prediction. The regression results showed that the marked ages of the three types of Chinese rice wine were successfully predicted using PLS and BP-ANN.
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Técnicas Eletroquímicas/métodos , Oryza/química , Vinho/análise , Análise de Variância , China , Análise por Conglomerados , Eletrodos , Análise dos Mínimos Quadrados , Análise de Componente PrincipalRESUMO
A voltammetric electronic tongue (VE-tongue) was developed to detect antibiotic residues in bovine milk. Six antibiotics (Chloramphenicol, Erythromycin, Kanamycin sulfate, Neomycin sulfate, Streptomycin sulfate and Tetracycline HCl) spiked at four different concentration levels (0.5, 1, 1.5 and 2 maximum residue limits (MRLs)) were classified based on VE-tongue by two pattern recognition methods: principal component analysis (PCA) and discriminant function analysis (DFA). The VE-tongue was composed of five working electrodes (gold, silver, platinum, palladium, and titanium) positioned in a standard three-electrode configuration. The Multi-frequency large amplitude pulse voltammetry (MLAPV) which consisted of four segments (1 Hz, 10 Hz, 100 Hz and 1000 Hz) was applied as potential waveform. The six antibiotics at the MRLs could not be separated from bovine milk completely by PCA, but all the samples were demarcated clearly by DFA. Three regression models: Principal Component Regression Analysis (PCR), Partial Least Squares Regression (PLSR), and Least Squares-Support Vector Machines (LS-SVM) were used for concentrations of antibiotics prediction. All the regression models performed well, and PCR had the most stable results.
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Antibacterianos/análise , Técnicas Eletroquímicas/métodos , Leite/química , Animais , Bovinos , Cloranfenicol/análise , Análise Discriminante , Eletrodos , Eritromicina/análise , Canamicina/análise , Análise dos Mínimos Quadrados , Neomicina/análise , Análise de Componente Principal , Estreptomicina/análise , Tetraciclina/análiseRESUMO
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