Single-cell RNA-seq reveals heterogeneity in metastatic renal cell carcinoma and effect of anti-angiogenesis therapy in the pancreas metastatic lesion.

Metastatic clear cell renal cell carcinoma has heterogenous tumor microenvironment (TME). Among the metastatic lesions, pancreas metastasis is rare and controversy in treatment approaches. Here, extensive primary and metastatic lesion samples were included by single-cell RNA-seq to decipher the distinct metastasis TME. The hypoxic and inflammatory TME of pancreas metastasis was decoded in this study, and the activation of PAX8-myc signaling, and metabolic reprogramming were observed. The active components including endothelial cells, fibroblasts and T cells were profiled. Meanwhile, we also evaluated the effect of anti-angiogenesis treatment in the pancreas metastasis patient. The potential mechanisms of pancreatic tropism, instability of genome, and the response of immunotherapy were also discussed in this work. Taken together, our findings suggest a clue to the heterogeneity in metastasis TME and provide evidence for the treatment of pancreas metastasis in renal cell carcinoma patients.

Impact of Stove Renovation on PM2.5 Exposure, Risk Perception, Self-Protective Willingness of Rural Residents.

To improve household air quality, the Chinese government has launched a number of pilot stove renovation projects, but few studies have explored the impact of the project on people's perception of and willingness to participate in these renovations; moreover, factors affecting willingness to pay for the project in rural China are not yet clear. We conducted a field measurement and a corresponding door-to-door questionnaire survey using the renovated group and the unrenovated group. The results showed that (1) the stove renovation project could not only reduce PM2.5 exposure and the excess mortality risk of rural residents, but also (2) improve residents' risk perception and self-protective willingness. (3) Specifically, the project had a deeper impact on female and low-income residents. (4) Meanwhile, the higher the income and the larger family size, the higher the risk perception and self-protective willingness. (5) Furthermore, willingness to pay for the project was related with residents' support for the project, benefit from renovation, income, and family size. Our results recommended that stove renovation policies should pay more attention to families with lower income and smaller size.

New observation of perceptive mechanism behind the long-lasting change of people's community mobility: evidence from COVID-19 in China.

COVID-19 pandemic provides an opportunity to investigate how a new and long-lasting threat affects public risk perception and social distancing behavior, which is important for pandemic risk management and recovery of the tertiary industry. We have found that the mechanism that perception decides behavior changes over time. At the beginning of the pandemic, risk directly shapes people's willingness of going out. But under a persistent threat, perception no longer plays the direct role of shape people's willingness. Instead, perception indirectly influences the willingness by shaping people's judgment about the necessity of traveling. Switching from direct to indirect influence, perception's effect is enlarged, which partially prevents people from returning to normal life even if the governmental ban is removed in a zero-COVID community.

LASSO-Based Pattern Recognition for Replenished Items With Graded Responses in Multidimensional Computerized Adaptive Testing.

As a branch of statistical latent variable modeling, multidimensional item response theory (MIRT) plays an important role in psychometrics. Multidimensional graded response model (MGRM) is a key model for the development of multidimensional computerized adaptive testing (MCAT) with graded-response data and multiple traits. This paper explores how to automatically identify the item-trait patterns of replenished items based on the MGRM in MCAT. The problem is solved by developing an exploratory pattern recognition method for graded-response items based on the least absolute shrinkage and selection operator (LASSO), which is named LPRM-GR and facilitates the subsequent parameter estimation of replenished items and helps maintaining the effectiveness of item replenishment in MCAT. In conjunction with the proposed approach, the regular BIC and weighted BIC are applied, respectively, to select the optimal item-trait patterns. Simulation for evaluating the LPRM-GR in pattern recognition accuracy of replenished items and the corresponding item estimation accuracy is conducted under multiple conditions across different numbers with respect to dimensionality, response-category numbers, latent trait correlation, stopping rules, and item selection criteria. Results show that the proposed method with the two types of BIC both have good performance in pattern recognition for item replenishment in the two- to four-dimensional MCAT with the MGRM, for which the weighted BIC is generally superior to the regular BIC. The proposed method has relatively high accuracy and efficiency in identifying the patterns of graded-response items, and has the advantages of easy implementation and practical feasibility.

Public anxiety through various stages of COVID-19 coping: Evidence from China.

As countries underwent the initiation, peak, post-peak, and early vaccination stages of COVID-19, the changing risk perception, coping behaviors and corresponding psychological stress experienced by the public over time was rarely reported. We conducted a national scale panel study using social-psychological data collected from 5,983 questionnaires to investigate the interactions between anxiety level, risk perception and coping behavior during different stages of COVID-19 in China. We found that sustained perceiving worries of being infected, first due to domestic and then global pandemic, contributed to the persistent high proportion of respondents with anxiety disorders which even gradually increased over time (56.1% during initiation to 60.4% during early vaccination). Gender was the strongest predictor of anxiety at all stages, with females having less confidence in COVID-19 control and always suffering from much higher anxiety levels than males even during the post peak stage. Excessive protective behavior and frequency of access to COVID-related news also contributed to public anxiety. Additionally, public risk perception was significantly associated with their willingness to vaccinate. The findings verify the feasibility of taking stage-specific and gender-based risk communication strategies to alleviate the pandemic-related public anxiety and promote vaccination by influencing public risk perception and guiding coping behaviors.

Surveillance of Parenting Outcomes, Mental Health and Social Support for Primiparous Women among the Rural-to-Urban Floating Population.

China has the largest population of floating rural-to-urban women worldwide, most of whom are of childbearing age. However, few studies have been conducted to monitor the changing trends in parenting outcomes, mental health and social support for these women in the early postpartum period. In this quantitative longitudinal study, 680 primiparous women among the floating population were recruited in Shenzhen, China. Face-to-face collection of socio-demographic questionnaires was completed by researchers in maternity wards on the third postnatal day. Follow-up electronic questionnaires were dispatched to women via email or WeChat at 6 weeks and 12 weeks following childbirth, including the Self-efficacy in Infant Care Scale (SICS), Edinburgh Postnatal Depression Scale (EPDS) and Postnatal Social Support Scale (PSSS), to measure maternal self-efficacy (MSE), postpartum depression (PPD) and social support, respectively. The mean scores of MSE for these floating women were 67.16 (14.35) at 6 weeks postpartum and slightly increased to 68.71 (15.00) at 12 weeks postpartum. The mean scores of EPDS remained almost stable, from 11.19 (4.89) to 11.18 (5.34) at the two time points. The prevalence of mild and severe PPD among floating women at 6 and 12 weeks after childbirth decreased from 54.4% to 40.1% and from 50.6% to 35.4%, respectively. The mean score of social support was 37.04 (10.15) at 6 weeks postpartum and slightly improved to 38.68 (10.46) at 12 weeks postpartum. Primiparous women among the rural-to-urban migrant population had an obviously negative status of parenting outcomes and mental health; and there was a lack of social support after childbirth. In future, tailored evidence-based interventions are highly needed to promote floating women's parenting outcomes, mental wellbeing and social support in the early stages of motherhood. As a higher-risk group of PPD, primiparous women among the floating population require effective and accessible mental health care after childbirth, such as early PPD screening and timely therapeutic methods.

Changes of public risk perception in China: 2008-2018.

This paper characterizes the risk acceptance of the Chinese public based on a psychometric paradigm and documents its change by conducting a nationally representative longitudinal survey spanning 10 years. We explore key factors that influence the acceptance of seven typical risks: drinking water pollution, interior decoration, electromagnetic radiation, air pollution, chemical plants, public transportation, and natural hazards, reflecting the general and referential changes in risk perception. The results show a general decrease in the acceptance of all of these risks in the examined decade, especially in economically developed areas. Different types of risk perception varied, but environmental risks had similar trends of perception. The perceived benefits from these risks and local GDP had the greatest impact on risk acceptance. The interaction between the changing perspectives of the emerging middle class and the evolving hazard risk landscape may be the reasons for the reduction in risk acceptance. The main findings offer insights for effective risk education and communication as well as sustainable risk management strategy.

Development of a Mobile Application of Internet-Based Support Program on Parenting Outcomes for Primiparous Women.

Primiparous women usually experience various parenting problems after childbirth that have negative effects on the well-being of mothers and infants. Although e-Support technology could provide an innovative and easily accessible intervention approach, mobile-phone interventions remain limited for Chinese primiparous women. Therefore, a new mobile application (APP) called the "Internet-based Support Program" ("ISP") was designed, incorporating the self-efficacy theory and the social-exchange theory for Chinese first-time mothers to improve their levels of maternal self-efficacy (MSE), social support, and satisfaction, as well as to reduce their postpartum depression symptoms. The research was conducted to develop and optimize the "ISP" APP for new mothers via a theory-, evidence-, and person-based approach. Five modules of "learning forum", "communication forum", "ask-the-expert forum", "baby home forum", and "reminder forum" were included in the APP to meet various parenting needs of first-time mothers; and its contents and functions were validated by the experts and primiparous women. The majority of participants gave positive feedback on the APP's perceived ease of use and usefulness. The "ISP" APP was the first designed for Chinese primiparous women, and a multicenter randomized controlled trial (RCT) will be conducted to measure its effectiveness on parenting outcomes.

Exploring Maternal Self-Efficacy of First-Time Mothers among Rural-to-Urban Floating Women: A Quantitative Longitudinal Study in China.

(1) Background: China has the highest number of rural-to-urban floating women in the world, and the majority of them are of childbearing age. However, few studies have focused on maternal self-efficacy (MSE) for these women. This research aims to explore MSE and its influencing factors for primiparous women among the rural-to-urban floating population in China. (2) Methods: A quantitative longitudinal study was conducted, and primiparous women from the floating population were recruited in China. Face-to-face demographic questionnaires were collected from obstetric wards by the researchers, three days postpartum. The 6-week and 12-week questionnaires, including the Self-efficacy in Infant Care Scale (SICS), the Edinburgh Postnatal Depression Scale (EPDS), and the Postpartum Social Support Scale (PSSS), were sent to participants by wechat or email to measure MSE, postnatal depression, and social support, respectively. The completed 6-week and completed 12-week questionnaires were returned to the researchers. (3) Results: The mean MSE scores at 6 and 12 weeks postpartum were 67.16 (SD = 14.35) and 68.71 (SD = 15.00). The variables of social support, postnatal depression, whether women attended parenting training, baby fussiness, baby health, maternal educational level, occupation, and family income affected MSE at the two time points. (4) Conclusions: Primiparous women among the rural-to-urban floating population had a low MSE level. As a vulnerable and special group, more attention should be paid to the negative parenting status of floating women by health workers and family members. Effective measures must be taken to improve the floating women's accessibility to parenting training from health services to strengthen their social support and alleviate postpartum depression. Health professionals should be more concerned with floating women with relatively low MSE levels, such as new mothers with lower education, poor working and living conditions, unhealthy babies, and babies with fussy temperaments.

Internet-based support program on parenting outcomes for Chinese primiparous women: Study protocol for a randomized controlled trial.

AIM: To evaluate the effects of internet-based support program for primiparous women in terms of improving the levels of maternal self-efficacy, social support, and satisfaction; and reducing their postpartum depression symptoms. DESIGN: A single-blinded, multicentre, randomized, controlled, parallel-group pre-test and repeated post-test design. METHODS: Based on the self-efficacy theory and the social exchange theory, the internet-based support program has five modules: (a) learning forum of parenting knowledge and skills; (b) communication forum; (c) ask-the-expert forum; (d) baby home forum; and (e) reminder forum. Primiparous women will be recruited in the obstetric wards of two university-affiliated hospitals in China. The participants (N = 258) will be randomly allocated to the intervention group that receive routine care and access to the internet-based support program and the control group that receive routine care during the 3 months postpartum. Maternal self-efficacy, social support, and postpartum depression symptoms will be measured at baseline, immediately after the intervention (post-test 1) and 3 months after the intervention (post-test 2). The study was funded in January 2018 and was ethically approved in May 2020. DISCUSSION: If the internet-based support program has positive outcomes, it will contribute to the scientific and practical knowledge of nursing interventions to support primiparous women on parenting; and could become the routine health care for health professionals to enhance parenting ability and mental well-being of new mothers. IMPACT: As the first RCT study on parenting outcomes using a rigorous research design and a theoretical framework in China, this research will contribute to evidence on the effectiveness of using internet platform to support women after childbirth. The results could help to advance research about the use of internet-based intervention methods to improve women's maternal self-efficacy, social support, satisfaction, and to alleviate depression symptoms. Chinese Clinical Trial Registry: ChiCTR2000033154.

[Determination of organic ultraviolet filters in environmental waters by magnetic solid phase extraction-high performance liquid chromatography].

A method for the determination of organic ultravioletfilters (OUFs) in water samples was developed by the combination of polymeric ionic liquid-based magnetic solid-phase extraction (PIL-MSPE) and high performance liquid chromatography-diode array detection (HPLC-DAD). To achieve the optimum extraction performance for the target analytes, several key variables, including desorption solvent, adsorption and desorption time, sample pH value and ionic strength in the sample matrix, were investigated in detail. Under the most favorable extraction conditions, linear behaviors were observed in the ranges of 0.5-200.0 µg/L for octyl salicylate and 0.2-200.0 µg/L for the other analytes. The limits of detection (LODs, S/N=3) and limits of quantification (LOQs, S/N=10) for the target OUFs were in the ranges of 0.009-0.13 µg/L and 0.031-0.43 µg/L, respectively. The developed PIL/MSPE-HPLC-DAD method was successfully applied to detect trace OUFs in real water samples. The spiked recoveries at different spiked levels were 71.4%-120%, and the RSDs were below 10% for all the compounds in all the samples. The proposed method has some unique merits including simple operation, fast extraction procedure, high sensitivity and environmental friendliness. It can become a useful method for the monitoring of trace OUFs in water samples.

A LASSO-Based Method for Detecting Item-Trait Patterns of Replenished Items in Multidimensional Computerized Adaptive Testing.

Multidimensional computerized adaptive testing (MCAT) is one of the widely discussed topics in psychometrics. Within the context of item replenishment in MCAT, it is important to identify the item-trait pattern for each replenished item, which indicates the set of the latent traits that are measured by each replenished item in the item pool. We propose a pattern recognition method based on the least absolute shrinkage and selection operator (LASSO) to detect the optimal item-trait patterns of the replenished items via an MCAT test. Simulation studies are conducted to investigate the performance of the proposed method in pattern recognition accuracy under different conditions across various latent trait correlation, item discrimination, test lengths, and item selection criteria in the test. Results show that the proposed method can accurately and efficiently identify the item-trait patterns of the replenished items in both the two-dimensional and three-dimensional item pools.

Preparation of highly fluorinated and boron-rich adsorbent for magnetic solid-phase extraction of fluoroquinolones in water and milk samples.

Effective extraction is an indispensable procedure for the sensitive analysis of fluoroquinolones (FQs) in complex samples. According to the molecular properties of FQs, a new highly fluorinated and boron-rich adsorbent (FBA) was synthesized and employed as the extraction phase of magnetic solid-phase extraction (MSPE). Results revealed that the prepared FBA displayed satisfactory extraction capability for FQs through fluorophilic and B-N coordination interactions. Besides, the synthesized FBA also exhibited strong magnetic responsiveness and good life-span. Under the most favorable conditions, the FBA/MSPE was combined with high-performance liquid chromatography with diode array detection (HPLC-DAD) to sensitively quantify trace levels of FQs in environmental water and milk samples. The developed approach showed good linearity within the studied concentration range, satisfactory precision and low limits of detection (0.0049-0.016 µg/L for water sample and 0.010-0.046 µg/kg for milk sample). In the analysis of target FQs in real samples, the recoveries of different fortified concentrations were in the ranges of 80.1-120% and 78.9-119% for water and milk samples, respectively. The relative standard deviations for reproducibility were all below 11%. The results well evidence that the introduced FBA/MSPE is a promising extraction technology for FQs, and the established FBA/MSPE-HPLC/DAD approach is suitable to measure low concentrations of FQs in water and milk samples.

Dual functional monomers modified magnetic adsorbent for the enrichment of non-steroidal anti-inflammatory drugs in water and urine samples.

According to the molecular properties of non-steroidal anti-inflammatory drugs (NSADs), a new adsorbent for magnetic solid phase extraction (MSPE) was designed and synthesized. Triethyl-(4-vinylbenzyl)aminium chloride and 4-vinylbenzeneboronic acid were utilized as dual functional monomers to copolymerize with divinylbenzene on the surface of pre-modified Fe3O4 nanoparticles. The prepared magnetic adsorbent (Fe3O4@TCVA) was characterized by elemental analysis, Fourier transform infrared, scanning electron microscopy, transmission electron microscopy and vibrating sample magnetometer. Due to the abundant boronic acid, quaternary amine and phenyl groups, the Fe3O4@TCVA displayed satisfactory extraction performance for target NSADs (diclofenac acid, ibuprofen and mefenamic acid) by means of B-N coordination, anion-exchange, π-π and hydrophobic interactions. Under the optimized conditions, the Fe3O4@TCVA/MSPE was combined with high-performance liquid chromatography with diode array detection (HPLC-DAD) to sensitively analyze NSADs in water and human urine samples. Results indicated that the limits of detection for water and urine samples were in the ranges of 0.014-0.031 µg/L and 0.029-0.11 µg/L, respectively. The relative standard deviations for the intra-day and inter-day assay variability were below 10%. The applicability of the proposed Fe3O4@TCVA/MSPE-HPLC-DAD method was demonstrated by the successful extraction and quantification of trace levels of NSADs in real water and human urine samples. Satisfactory spiked recovery and reproducibility were achieved.

Direct/Alternating Current Electrochemical Method for Removing and Recovering Heavy Metal from Water Using Graphene Oxide Electrode.

Treatment of heavy-metal pollution in both point-of-use water and industrial wastewater is critical in protecting human health and the environment. Current methods for heavy-metal treatment in both sources have limitations. For point-of-use water, current methods usually suffer from limited capacity and difficulties in spontaneously removing multiple heavy metals. For industrial wastewater, current methods greatly reduce the value of heavy metal by precipitating them as sludge which requires further treatment. Here we developed an electrochemical method that can treat both low-concentration and high-concentration heavy-metal pollution using either direct current (DC) or alternating current (AC) electrodeposition with graphene-oxide-modified carbon felt electrode (CF-GO). The graphene oxide provides a high density of surface functional groups to assist the electrodeposition. The electrodeposition method showed 2 orders of magnitude higher capacity (>29 g heavy metal for 1 g of graphene oxide) compared with traditional adsorption methods. For low levels of heavy-metal pollution in point-of-use water, DC electrodeposition with a CF-GO electrode can reduce single heavy-metal ion pollution (Cu, Cd, and Pb) as well as multiple ion mixtures to below safe water drinking levels. This method can tolerate a much wider range of heavy-metal pollution in point-of-use water than traditional adsorption methods. For high-level pollution in industrial wastewater, AC electrodeposition can recover >99.9% heavy-metal ions. By tuning the AC frequency and voltage, the electrodeposition method can further selectively recover Cu, Cd, and Pb separately, which adds values to the heavy-metal removal process.

Metal-organic framework-monolith composite-based in-tube solid phase microextraction on-line coupled to high-performance liquid chromatography-fluorescence detection for the highly sensitive monitoring of fluoroquinolones in water and food samples.

In this study, a new metal-organic framework-monolith composite for in-tube solid phase microextraction phase (IT-SPME) of fluoroquinolones (FQs) was prepared. 4-Vinylbenzoic acid was copolymerized with ethylenedimethacrylate in a fused silica capillary to form porous monolith. After that, zeolitic imidazolate frameworks (ZIF-8) were synthesized in situ within the pores and the surface of the monolith by controlled layer-by-layer self-assembly of Zn2+ and imidazole. The introduction of ZIF-8 enhanced the surface area of monolith composite, and thus, improving the extraction performance of IT-SPME for FQs obviously. Under the optimized conditions, a highly sensitive method for the monitoring of FQs residue in water and honey samples was developed by the on-line combination of IT-SPME with high-performance liquid chromatography with fluorescence detection (HPLC-FLD). The limits of detection (S/N = 3) for the targeted FQs in water and honey samples were as low as 0.14-0.61 ng/L and 0.39-1.1 ng/L, respectively. The relative standard deviations (RSDs) for intra-day and inter-day assay variability were less than 10% in all samples. The established on-line IT-SPME-HPLC-FLD was successfully used to detect ultra-trace FQs in environmental water and honey samples. Recoveries at different spiked concentrations ranged from 80.1% to 119% and 80.2-117% for water and honey samples, respectively, with satisfactory reproducibility. Compared to up-to-date reported methods, the proposed approach exhibits some features such as high sensitivity, convenience, partial automation, low consumptions of sample and solvent.

Temperature relations of aerial and aquatic physiological performance in a mid-intertidal limpet Cellana toreuma: adaptation to rapid changes in thermal stress during emersion.

The physiological performance of a mid-intertidal limpet Cellana toreuma was determined to study the physiological adaptation of intertidal animals to rapid changes and extreme temperatures during emersion. The relationship between the Arrhenius breakpoint temperature (ABT) and in situ operative body temperature was studied to predict the possible impact of climate change on the species. The temperature coefficient (Q10) of emersed animals was higher than that of submersed animals and the ratio of aerial: aquatic heart rate rose with increasing temperature. The ABTs of submersed and emersed animals were 30.2 and 34.2°C, respectively. The heart rate and levels of molecular biomarkers (hsps, ampkα, ampkß and sirt1 mRNA) were determined in 48 h simulated semi-diurnal tides. There were no obvious changes of heart rate and gene expression during the transition between emersion and submersion at room temperature, although expressions of hsp70 and hsp90 were induced significantly after thermal stress. These results indicate that C. toreuma can effectively utilize atmospheric oxygen, and the higher Q10 and ABT of emersed animals are adaptations to the rapid change and extreme thermal stress during emersion. However, the in situ operative body temperature frequently exceeds the aerial ABT of C. toreuma, indicating the occurrence of large-scale mortality of C. toreuma in summer, and this species should be sensitive to increasing temperature in the scenario of climate change.