A Europium Nanosphere-Based Time-Resolved Fluorescent Immunochromatographic Assay for the Rapid Screening of 4,4'-Dinitrocarbanilide: Aiming at Improving Strip Method Performance.

Considering that the strip method is simple and convenient for users, a Europium nanosphere-based time-resolved fluorescent immunochromatographic assay (TRFICA) for the rapid screening of 4,4'-dinitrocarbanilide (DNC) was developed to improve the performance of strip assays. After optimization, TRFICA showed IC50, the limit of detection, and cut-off values of 0.4, 0.07, and 5.0 ng mL-1, respectively. No significant cross-reactivity (CR < 0.1%) with 15 DNC analogs was observed in the developed method. TRFICA was validated for DNC detection in spiked chicken homogenates, and recoveries ranged from 77.3% to 92.7%, with coefficients of variation of <14.9%. Moreover, the time needed for the detection procedure, including the sample pre-treatment, was less than 30 min for TRFICA, which had never been achieved before in other immunoassays. The newly developed strip test is a rapid, sensitive, quantitative, and cost-effective on-site screening technique for DNC analysis in chicken muscle.

Theoretical and experimental research on valve-less piezoelectric pump with arc-shaped tubes.

In order to apply a valve-less piezoelectric pump in navigation equipment, this work first revealed a valve-less piezoelectric pump with arc-shaped tubes and then researched the relationship between the main structural parameters of arc-shaped tubes and the output flow rate. According to the velocity difference between clockwise and counterclockwise directions of liquid in circular arc-shaped tubes, the flow characteristics of liquid in circular arc-shaped tubes are analyzed in this paper, and the velocity difference model and flow equation of a piezoelectric pump are established. By using three-dimensional printing technology, five types of prototypes with different parameters were made, and the experimental research was carried out. The results show that at the optimal frequency, the pump output flow is the largest. The output flow is proportional to the driving voltage, the base circle radius, and the cross section area of the pipe. The low-cost and miniaturized navigation equipment made by using the piezoelectric pump has potential application value in unmanned aerial vehicle and intelligent vehicle navigation.