Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 9 de 9
Filtrar
Mais filtros










Intervalo de ano de publicação
1.
Rev Esp Patol ; 52(4): 222-233, 2019.
Artigo em Espanhol | MEDLINE | ID: mdl-31530405

RESUMO

Pathology and clinical oncology work hand in hand so that techniques and treatments, biomarkers and antibodies share the common goal of identifying integral new treatment regimens that are more effective and less aggressive. Evidence shows how tissue mechanics affect carcinogenesis and that tumor heterogeneity depends on metabolic stromal alteration and the Warburg effect of malignant cells, regulated directly by PD-1, becoming a target for immunotherapy. Proliferation and apoptosis depend on mitochondrial dysfunction in tumor cells, determining the grade of chemo/radio-resistance. The status of intestinal microbiota regulates immune response, tumor microenvironment structure and oncologic treatment response, whilst the Vitamin D receptor allows reprogramming of tumor stroma. Current collaboration between basic and clinical research paves the way for future investigation into areas such as tumor microenvironment and molecular mechanotherapy, metabolism and immunotherapy, mitochondria and oncogenesis, microbiota and chemotherapy, psychoneuroendocrine axis and homeostatic imbalance, epigenetics and reprogramming possibilities of the tumor phenotype. We review new prognostic and predictive biomarkers emerging from these fields of knowledge, opening up new therapeutic possibilities.

2.
Rev. esp. patol ; 52(2): 92-102, abr.-jun. 2019. ilus, tab
Artigo em Espanhol | IBECS | ID: ibc-182695

RESUMO

El grupo de enfermedades al que nos referimos como «cáncer» comparte una estructura biológica conformada por un ecosistema complejo, donde se han alterado las relaciones intercelulares, los campos de información, el desarrollo y la función tisular. Más allá de las alteraciones genéticas de la célula tumoral, la demostración de un ecosistema alterado, con sus interconexiones a nivel sistémico, abre una nueva perspectiva de la biología y del comportamiento del cáncer. Diversas facetas del tumor, su morfología, clasificación, agresividad clínica, pronóstico y respuesta al tratamiento aparecen ahora bajo una visión integral que ofrece un nuevo horizonte de estudio, investigación y manejo clínico. La Teoría de la Mutación Somática en cáncer, vigente desde hace más de 100 años, se ve hoy completada por el estudio del microambiente tumoral, la matriz extracelular, las células estromales, la respuesta inmune, la inervación, la nutrición, la mitocondria, el metabolismo, el fluido intersticial, las propiedades mecánicas y electromagnéticas del tejido, y muchas otras áreas de conocimiento emergente, que abren la puerta a un ejercicio de reprogramación del fenotipo tumoral a través de la modificación de las claves ofrecidas por este nuevo paradigma. Su reconocimiento permite pasar de considerar el proceso oncológico como un problema celular a una alteración supracelular basada en la desorganización de los tejidos, inmersos en las relaciones del sistema complejo que conforma un ser vivo


The group of diseases that we call cancer share a biological structure formed by a complex ecosystem, with altered intercellular communication, information fields, development and tissue function. Beyond the genetic alterations of the tumor cell, the demonstration of an altered ecosystem, with interconnections at systemic levels, opens up a new perspective on cancer biology and behavior. Different tumor facets, such as morphology, classification, clinical aggressiveness, prognosis and response to treatment now appear under a comprehensive vision that offers a new horizon of study, research and clinical management. The Somatic Mutation Theory in cancer, in force for more than one hundred years, is now completed by the study of the tumor microenvironment, the extracellular matrix, the stromal cells, the immune response, the innervation, the nutrition, the mitochondria, the metabolism, the interstitial fluid, the mechanical and electromagnetic properties of the tissue and many other areas of emerging knowledge; thus opening the door to a reprogramming exercise of the tumor phenotype through the modification of the keys offered by this new paradigm. Its recognition makes it possible to go from considering the oncological process as a cellular problem to a supracellular alteration based on the disorganization of tissues, immersed in the relationships of the complex system of the living being


Assuntos
Humanos , Neoplasias/classificação , Gradação de Tumores/métodos , Microambiente Tumoral , Técnicas de Reprogramação Celular/tendências , Matriz Extracelular/patologia , Taxa de Mutação
4.
Rev Esp Patol ; 52(2): 92-102, 2019.
Artigo em Espanhol | MEDLINE | ID: mdl-30902384

RESUMO

The group of diseases that we call cancer share a biological structure formed by a complex ecosystem, with altered intercellular communication, information fields, development and tissue function. Beyond the genetic alterations of the tumor cell, the demonstration of an altered ecosystem, with interconnections at systemic levels, opens up a new perspective on cancer biology and behavior. Different tumor facets, such as morphology, classification, clinical aggressiveness, prognosis and response to treatment now appear under a comprehensive vision that offers a new horizon of study, research and clinical management. The Somatic Mutation Theory in cancer, in force for more than one hundred years, is now completed by the study of the tumor microenvironment, the extracellular matrix, the stromal cells, the immune response, the innervation, the nutrition, the mitochondria, the metabolism, the interstitial fluid, the mechanical and electromagnetic properties of the tissue and many other areas of emerging knowledge; thus opening the door to a reprogramming exercise of the tumor phenotype through the modification of the keys offered by this new paradigm. Its recognition makes it possible to go from considering the oncological process as a cellular problem to a supracellular alteration based on the disorganization of tissues, immersed in the relationships of the complex system of the living being.

7.
Leuk Lymphoma ; 46(11): 1581-91, 2005 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-16236613

RESUMO

The present study aimed to describe the general tissular composition of the immune infiltrate observed in Hodgkin's lymphoma (HL) and its possible relationship with clinical and survival prognostic factors. In this retrospective study of 267 HL patients, the relative proportions of infiltrating T lymphocytes (CD4+, CD8+), natural killer cells (CD 56+, CD 57+), cytotoxic cells (Granzyme B+, TIA-1+) and dendritic cells (CD 21+, S-100+) were quantified immunohistochemically with tissue microarray technology. Our results confirm the predominance of CD4 + T lymphocytes in the background of tumoral cells, in addition to a high number of cytotoxic lymphocytes (CD8, CD 57 and TIA-1). Patients with low numbers of infiltrating CD8, CD 56, CD 57+cells and high numbers of Granzyme B and TIA-1+cells presented a significantly unfavourable clinical course (presence of leukocytosis, B symptoms, advanced clinical stage (III/IV), non-responding patients). A reduced infiltration of CD4+T lymphocytes was related with the presence of Epstein - Barr virus. Significantly longer survival times were observed in patients with a high level of infiltrating CD 57, as well as a low level of Granzyme B and TIA-1+cells (log-rank test). When evaluated in a multivariate model, high levels of infiltrating TIA-1 and Granzyme B+cells were shown to be independent prognostic factors that negatively influenced overall survival. The presence of TIA-1+cells was found to be the only unfavorable prognostic factor of event-free survival and disease-free survival. The overall detection of tumor-infiltrating cells in HL confirms the importance of cytotoxic T lymphocyte infiltration (Granzyme B and TIA-1+cells) in these patients. Independently of the classical clinical and pathological features, these cells appear to be an unfavourable prognostic factor in HL and, more particularly, the presence of cytotoxic TIA-1+cells.


Assuntos
Doença de Hodgkin/patologia , Invasividade Neoplásica/patologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Contagem de Células , Criança , Células Dendríticas/patologia , Feminino , Granzimas , Doença de Hodgkin/mortalidade , Humanos , Imuno-Histoquímica , Células Matadoras Naturais/patologia , Masculino , Pessoa de Meia-Idade , Proteínas de Ligação a Poli(A) , Prognóstico , Proteínas de Ligação a RNA/análise , Estudos Retrospectivos , Serina Endopeptidases/análise , Análise de Sobrevida , Antígeno-1 Intracelular de Células T , Linfócitos T/patologia , Linfócitos T Citotóxicos/patologia , Análise Serial de Tecidos
8.
Ann Hematol ; 84(10): 661-6, 2005 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-15875183

RESUMO

This study aimed to assess the differences in the cellular composition of the inflammatory reactive background around tumoral cells of classical Hodgkin's lymphomas (cHL) inside and outside the HIV settings. This retrospective study evaluates the infiltrating T lymphocytes (CD4 and CD8), natural killer cells (CD57+ cells), and more especially cytotoxic cells [granzyme B (GrB) and TIA-1+ cells] in the background of 99 EBV+ cHL. Sections from paraffin-embedded tumor samples from nine HIV-infected cHL patients were immunostained, using standard immunohistochemical protocols and were compared to a control group of 90 HIV-noninfected cHL patients. Our clinical and histological data indicate that HIV-infected cHL patients present a higher frequency of mixed cellularity (MC) histological subtypes, more advanced disease stages, a poor response to treatment, and a poor overall survival compared to control patients. In controls, CD4/CD8 and GrB/TIA-1 ratios were determined as 2:1 and 1:2, respectively. The inflammatory infiltrate of HIV-infected patients had a significant reduction of CD4+ T lymphocytes (CD4/CD8 ratio 1:23), a decrease in infiltrating GrB+ cells (activated cytotoxic cells) and an increase in infiltrating TIA+ T cells (mainly nonactivated cytotoxic cells) in these patients (GrB/TIA-1 ratio 1:12). In conclusion, this study highlights an important intratumoral loss of CD4+ T cells (striking inversion in the CD4/CD8 ratio) and a decrease in intratumoral activated cytotoxic T lymphocytes in HIV-associated cHL patients. Further studies are required to confirm these results and to determine the role of these findings on the antitumoral immune response observed in HIV-associated cHL.


Assuntos
Biomarcadores Tumorais/metabolismo , Linfócitos T CD8-Positivos/metabolismo , Infecções por HIV/metabolismo , HIV , Doença de Hodgkin/metabolismo , Serina Endopeptidases/metabolismo , Adulto , Relação CD4-CD8/métodos , Linfócitos T CD4-Positivos/metabolismo , Linfócitos T CD4-Positivos/patologia , Linfócitos T CD4-Positivos/virologia , Linfócitos T CD8-Positivos/patologia , Linfócitos T CD8-Positivos/virologia , Feminino , Granzimas , Infecções por HIV/complicações , Infecções por HIV/mortalidade , Infecções por HIV/patologia , Doença de Hodgkin/mortalidade , Doença de Hodgkin/patologia , Doença de Hodgkin/virologia , Humanos , Inflamação/metabolismo , Inflamação/patologia , Inflamação/virologia , Masculino , Pessoa de Meia-Idade , Proteínas de Ligação a Poli(A) , Proteínas de Ligação a RNA/metabolismo , Estudos Retrospectivos , Antígeno-1 Intracelular de Células T
9.
Med. clín (Ed. impr.) ; 114(11): 411-413, mar. 2000.
Artigo em Espanhol | IBECS | ID: ibc-6333

RESUMO

Fundamento: Se pretende determinar la proporción de enfermedad de Hodgkin (EH) que expresa el virus de Epstein-Barr (VEB) en nuestro medio. Pacientes y métodos: Se ha realizado un estudio retrospectivo sobre 49 casos de EH usando la técnica inmunohistoquímica LMP-1 y la técnica de hibridación in situ para EBER-1. Resultados: Un 40,8 por ciento (20/49) de los casos expresaba VEB (EBER-1 y/o LMP-1 positivos). Este porcentaje fue significativamente mayor en EH diagnosticadas a pacientes mayores de 55 años y no hubo diferencias por sexo, aunque fue mayor, pero no de forma significativa, en el subtipo histológico de EH de celularidad mixta. Conclusiones: El VEB se asocia a un 40,8 por ciento de las EH en las comarcas de Tarragona (AU)


Assuntos
Pessoa de Meia-Idade , Criança , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Masculino , Feminino , Humanos , Herpesvirus Humano 4 , Espanha , Estudos Retrospectivos , Doença de Hodgkin
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA