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1.
Artigo em Inglês | MEDLINE | ID: mdl-32383030

RESUMO

The antioxidant and cardioprotective effects of oleuropein have been reported in several studies; however, its effect on ketamine cardiotoxicity has not been known yet. The aim of this study was to investigate the effects of oleuropein in ketamine-induced cardiotoxicity model in rats. A total of 28 male Wistar Albino rats were included in the study and they were randomly divided into four groups, each having seven rats. Group 1 (control): rats were given 1 mL of DMSO by oral gavage method for 7 days. Group 2 (ketamine): on the seventh day of the study, 60 mg/kg ketamine was administered intraperitoneally. Then, 60 mg/kg ketamine was administered intraperitoneally every 10 min for 3 h. Group 3 (oleuropein): rats were given 200 mg/kg/day oleuropein by oral gavage method for 7 days. Group 4 (oleuropein + ketamine): rats were given 1 × 200 mg/kg oleuropein by oral gavage method for 7 days. Furthermore, 60 mg/kg ketamine was administered intraperitoneally on the seventh day of the experiment. Then, 60 mg/kg ketamine was administered intraperitoneally every 10 min for 3 h. Serum cardiac marker (TnI, CK-MB and CK) levels were measured. Histopathological analysis was performed on a portion of the cardiac tissue. Cardiac tissue oxidative stress and antioxidant markers (MDA, GSH, GSH.Px and CAT), TNF-α, IL-6, NF-κB, COX-2 and Nrf-2 gene expressions, and protein conversion levels of related genes were determined. Data obtained showed that ketamine administration increased MDA (p < 0.001), TNF-α (p < 0.01), IL-6 (p < 0.01), COX-2 (p < 0.001) and NF-κB (p < 0.001) levels, as well as serum TnI (p < 0.001), CK-MB (p < 0.001) and CK (p < 0.01) levels whereas decreased GSH (p < 0.05) and Nrf-2 (p < 0.05) levels, as well as GSH-Px (p < 0.001) and CAT (p < 0.05) enzyme activities. Oleuropein administration was observed to decrease MDA, TNF-α, IL-6, COX-2, NF-κB, TnI, CK-MB and CK levels close to the control group and to increase GSH levels and GSH-Px and CAT enzyme activities close to the control group. This study showed that oleuropein administration reversed the increased oxidative stress and inflammation as a result of the use of ketamine and had protective effects on the heart.

2.
Inflammation ; 43(1): 336-346, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31705353

RESUMO

Ischemia-reperfusion injury is an organ failure caused by hypoxia and reperfusion, which is closely associated with oxidative stress and inflammation. In this study, we investigated whether nobiletin had protective effects on inflammatory parameters, oxidative damage, iNOS-eNOS expressions, and histopathological structure of renal tissue in rats with renal ischemia-reperfusion injury. For this purpose, 24 rats were divided into 4 groups: group 1 (Control), group 2 (Ischemia-Reperfusion-IR), group 3 (Nobiletin-10 mg/kg p.o.), group 4 (Nobiletin + IR). The study was continued for 7 days. At the end of the study, urea (p < 0.05), creatine (p < 0.05), MDA (p < 0.001), TNF-alpha (p < 0.001), IL-1 beta (p < 0.05), and IL-6 (p < 0.001) levels increased in the IR group; however, a significant decrease occurred in group 4 (Nobiletin + IR) and it reached the control group levels. In the IR group, GSH (p < 0.01) levels, and GSH.Px (p < 0.01) and CAT (p < 0.05) activities decreased whereas they increased significantly in group 4 (Nobiletin + IR) and reached the same levels as the control group. In histopathological analyses, destruction and increased iNOS-eNOS expressions in the IR group showed a significant decrease in group 4 (Nobiletin + IR). As a result, the application of nobiletin has shown that it has protective effects by reducing kidney damage caused by IR injury.

3.
J Biochem Mol Toxicol ; 34(2): e22427, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31777137

RESUMO

The study aimed to examine the effects of nobiletin on the toxicity model induced with acetaminophen (APAP). For this purpose, 24 adult male rats were equally divided into four groups. The groups were the control group (group 1); dimethyl sulfoxide only, the APAP group (group 2) received a single dose of APAP 1000 mg/kg on the 10th day of experiment; the Nobiletin group (group 3), nobiletin (10 mg/kg) for 10 days; and the APAP + Nobiletin group (group 4), nobiletin (10 mg/kg) for 10 days with a single dose of APAP (1000 mg/kg) administered on the 10th day and the experiment ended after 48 hours. At the end of the study, a significant increase in malondialdehyde, interleukin-1ß (IL-1ß), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) levels and a significant decrease in glutathione levels, glutathione peroxidase activities and nuclear factor erythroid-derived 2-like 2 (Nrf-2) and heme oxygenase-1 (HO-1) expressions were observed with APAP application in liver and kidney tissues. Serum aspartate transaminase (AST), alanine transaminase (ALT), urea, and creatinine levels were also significantly increased in the APAP group. However, nobiletin treatment in group 4 reversed oxidative stress and inflammatory and histopathological signs caused by APAP. It is concluded that nobiletin may be a beneficial substance that confers hepatorenal protection to APAP-induced toxicity via antioxidant and anti-inflammatory mechanisms.


Assuntos
Acetaminofen/efeitos adversos , Acetaminofen/farmacologia , Anti-Inflamatórios/farmacologia , Antioxidantes/farmacologia , Flavonas/farmacologia , Rim/efeitos dos fármacos , Fígado/efeitos dos fármacos , Alanina Transaminase/sangue , Animais , Anti-Inflamatórios/uso terapêutico , Antioxidantes/uso terapêutico , Aspartato Aminotransferases/sangue , Doença Hepática Induzida por Substâncias e Drogas/tratamento farmacológico , Creatinina/sangue , Citocinas/metabolismo , Flavonas/uso terapêutico , Glutationa/metabolismo , Glutationa Peroxidase/metabolismo , Heme Oxigenase (Desciclizante)/metabolismo , Rim/metabolismo , Rim/patologia , Fígado/metabolismo , Fígado/patologia , Masculino , Malondialdeído/metabolismo , Fator 2 Relacionado a NF-E2/metabolismo , Estresse Oxidativo/efeitos dos fármacos , Ratos , Ratos Wistar , Ureia/sangue
4.
5.
Ann Hepatol ; 18(5): 715-724, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31204236

RESUMO

INTRODUCTION AND OBJECTIVES: The objectives of this study were to investigate the underlying mechanism of PPARα, LXRα, ChREBP, and SREBP-1c at the level of gene and protein expression with high-energy diets in liver and skeletal muscle. MATERIALS AND METHODS: Metabolic changes with consumption of high fat (Hfat), high sucrose (Hsuc) and high fructose (Hfru) diets were assessed. Levels of mRNA and protein of PPARα, LXRα, ChREBP, and SREBP-1c were investigated. Body weight changes, histological structure of liver and plasma levels of some parameters were also examined. RESULTS: In Hfru group, body weights were higher than other groups (P<0.05). In liver, LXRα levels of Hsuc and Hfru groups were upregulated as 1.87±0.30 (P<0.05) and 2.01±0.29 (P<0.01). SREBP-1c levels were upregulated as 4.52±1.25 (P<0.05); 4.05±1.11 (P<0.05) and 3.85±1.04 (P<0.05) in Hfat, Hsuc, and Hfru groups, respectively. In skeletal muscle, LXRα and SREBP-1c were upregulated as 1.77±0.30 (P<0.05) and 2.71±0.56 (P<0.05), in the Hfru group. Protein levels of ChREBP (33.92±8.84ng/mg protein (P<0.05)) and SREBP-1c (135.16±15.57ng/mg protein (P<0.001)) in liver were higher in Hfru group. In skeletal muscle, LXRα, ChREBP and SREBP-1c in Hfru group were 6.67±0.60, 7.11±1.29 and 43.17±6.37ng/mg, respectively (P<0.05; P<0.01; P<0.05). The rats in Hfru group had the most damaged livers. CONCLUSION: Besides liver, fructose consumption significantly effects skeletal muscle and leads to weight gain, triggers lipogenesis and metabolic disorders.

6.
Inflammation ; 42(5): 1680-1691, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31115770

RESUMO

In this study, the effects of tyrosol were investigated in DSS-induced experimental ulcerative colitis model. For this purpose, rats were divided into five groups of seven rats in each: control group, colitis group (DSS-4%), tyrosol group (tyrosol 20 mg/kg), sulfasalazine (sulfasalazine+DSS 100 mg/kg), and treatment group (tyrosol+DSS 20 mg/kg). In the study, the active substances were administered to all animals for a period of 21 days. At the end of the study, malondialdehyde (MDA) levels increased (p < 0.001); GSH level (p < 0.05) along with GSH.Px (p < 0.01) and CAT (p < 0.001) activities decreased in the DSS-induced colitis group. However, with the administration of tyrosol, MDA and GSH levels along with GSH.Px and CAT activities came to the same levels as the control group. In the colitis group, an increase occurred in IL-6, COX-2, and NF-κB parameters, which created a significant difference compared to the control group (p < 0.001). Similarly, TNF-α levels also significantly increased with the administration of DSS (p < 0.05) which created a significant difference compared to the control group, while there was no difference among the other groups. As for the Nrf-2 data, it decreased with the administration of DSS which created a significant difference compared to the control group (p < 0.05), while there was no difference in other groups. In the colitis-induced group, IL-6, COX-2, and NF-κB gene expression levels also similarly increased but returned to the normal levels with the administration of tyrosol. In the histopathological scoring, the negativity that increased with the administration of DSS returned to the normal levels with the administration of tyrosol+DSS. In conclusion, according to the data obtained, tyrosol fixed the destruction picture in the DSS-induced colitis model, giving rise to thought that it has a protective effect.


Assuntos
Antioxidantes/uso terapêutico , Colite Ulcerativa/tratamento farmacológico , Álcool Feniletílico/análogos & derivados , Substâncias Protetoras/uso terapêutico , Animais , Antioxidantes/farmacologia , Colite Ulcerativa/induzido quimicamente , Ciclo-Oxigenase 2/metabolismo , Sulfato de Dextrana , Glutationa/efeitos dos fármacos , Glutationa/metabolismo , Malondialdeído/metabolismo , NF-kappa B/metabolismo , Álcool Feniletílico/farmacologia , Álcool Feniletílico/uso terapêutico , Substâncias Protetoras/farmacologia , Ratos , Fator de Necrose Tumoral alfa/metabolismo
7.
Sci Transl Med ; 11(491)2019 05 08.
Artigo em Inglês | MEDLINE | ID: mdl-31068441

RESUMO

Mechanisms that govern the shift from joint homeostasis to osteoarthritis (OA) remain unknown. Here, we identify a pathway used for joint development and homeostasis, and its role in OA. Using a combination of transgenic, pharmacological, and surgical conditions in mouse and human tissues, we found that TGF-ß signaling promotes joint homeostasis through regulation of the IL-36 family. We identified IL-36 receptor antagonist (IL-36 in mice and IL-36RN in humans) as a potential disease-modifying OA drug. Specifically, OA development was associated with IL-36α up-regulation and IL-36Ra down-regulation in mice with tissue-specific postnatally induced ablation of Tgfbr2, mice treated with a TGF-ß signaling inhibitor, mice with posttraumatic OA, and aging mice with naturally occurring OA. In human cartilage, OA severity was associated with decreased TGFBR2 and IL-36RN, whereas IL-36α increased. Functionally, intra-articular treatment with IL-36Ra attenuated OA development in mice, and IL-36RN reduced MMP13 in human OA chondrocytes. These findings highlight the relevance of TGFBR2-IL-36 interplay in joint homeostasis and IL-36RN as a potential therapeutic agent for OA.

8.
IEEE Trans Neural Netw Learn Syst ; 30(3): 923-937, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30072350

RESUMO

We propose an online algorithm for sequential learning in the contextual multiarmed bandit setting. Our approach is to partition the context space and, then, optimally combine all of the possible mappings between the partition regions and the set of bandit arms in a data-driven manner. We show that in our approach, the best mapping is able to approximate the best arm selection policy to any desired degree under mild Lipschitz conditions. Therefore, we design our algorithm based on the optimal adaptive combination and asymptotically achieve the performance of the best mapping as well as the best arm selection policy. This optimality is also guaranteed to hold even in adversarial environments since we do not rely on any statistical assumptions regarding the contexts or the loss of the bandit arms. Moreover, we design an efficient implementation for our algorithm using various hierarchical partitioning structures, such as lexicographical or arbitrary position splitting and binary trees (BTs) (and several other partitioning examples). For instance, in the case of BT partitioning, the computational complexity is only log-linear in the number of regions in the finest partition. In conclusion, we provide significant performance improvements by introducing upper bounds (with respect to the best arm selection policy) that are mathematically proven to vanish in the average loss per round sense at a faster rate compared to the state of the art. Our experimental work extensively covers various scenarios ranging from bandit settings to multiclass classification with real and synthetic data. In these experiments, we show that our algorithm is highly superior to the state-of-the-art techniques while maintaining the introduced mathematical guarantees and a computationally decent scalability.

9.
J Chin Med Assoc ; 81(9): 811-815, 2018 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-29778552

RESUMO

BACKGROUND: Data describing how laryngeal cancer affects oxidative stress markers and antioxidants are limited. This study investigated serum antioxidant enzyme activities and oxidative stress markers before and after laryngectomies in patients with laryngeal cancer. METHODS: A total of 29 patients with laryngeal cancer and 25 healthy control subjects were enrolled. Serum malondialdehyde (MDA) levels and superoxide dismutase (SOD), glutathione peroxidase (GSHPx), catalase (CAT), paraoxonase (PON), and arylesterase activities were measured spectrophotometrically. Blood samples were obtained from each patient just before surgery and 1 month after a laryngectomy. RESULTS: The serum PON, arylesterase, CAT, SOD, and GSHPx activities were significantly decreased (all p < 0.001) and serum MDA levels were significantly increased (p < 0.001) in patients with laryngeal cancer, compared with control subjects. In laryngeal cancer patients, the serum GSHPx and arylesterase activity levels increased significantly following laryngectomies (both p < 0.001), whereas the MDA levels decreased significantly (p = 0.007). CONCLUSION: In patients with laryngeal cancer, the oxidant/antioxidant balance shifted toward oxidative stress. In addition, following laryngectomies, laryngeal cancer patients had increases in serum antioxidant enzyme activities and decreases in oxidative stress markers.


Assuntos
Neoplasias Laríngeas/metabolismo , Estresse Oxidativo , Idoso , Arildialquilfosfatase/sangue , Catalase/sangue , Feminino , Glutationa Peroxidase/sangue , Humanos , Neoplasias Laríngeas/cirurgia , Laringectomia , Masculino , Malondialdeído/sangue , Pessoa de Meia-Idade , Superóxido Dismutase/sangue
10.
Acta Biomater ; 73: 263-274, 2018 06.
Artigo em Inglês | MEDLINE | ID: mdl-29656073

RESUMO

Osteoarthritis (OA) is a condition where tissue function is lost through a combination of secondary inflammation and deterioration in articular cartilage. One of the most common causes of OA is age-related tissue impairment because of wear and tear due to mechanical erosion. Hyaluronic acid-based viscoelastic supplements have been widely used for the treatment of knee injuries. However, the current formulations of hyaluronic acid are unable to provide efficient healing and recovery. Here, a nanofiber-hyaluronic acid membrane system that was prepared by using a quarter of the concentration of commercially available hyaluronic acid supplement, Hyalgan®, was used for the treatment of an osteoarthritis model, and Synvisc®, which is another commercially available hyaluronic acid containing viscoelastic supplement, was used as a control. The results show that this system provides efficient protection of arthritic cartilage tissue through the preservation of cartilage morphology with reduced osteophyte formation, protection of the subchondral region from deterioration, and maintenance of cartilage specific matrix proteins in vivo. In addition, the hybrid nanofiber membrane enabled chondrocyte encapsulation and provided a suitable culturing environment for stem cell growth in vitro. Overall, our results suggest that this hybrid nanofibrous scaffold provides a potential platform the treatment of OA. STATEMENT OF SIGNIFICANCE: Osteoarthritis is a debilitating joint disease affecting millions of people worldwide. It occurs especially in knees due to aging, sport injuries or obesity. Although hyaluronic acid-based viscoelastic supplements are widely used, there is still no effective treatment method for osteoarthritis, which necessitates surgical operation as an only choice for severe cases. Therefore, there is an urgent need for efficient therapeutics. In this study, a nanofiber-HA membrane system was developed for the efficient protection of arthritic cartilage tissue from degeneration. This hybrid nanofiber system provided superior therapeutic activity at a relatively lower concentration of hyaluronic acid than Hyalgan® and Synvisc® gels, which are currently used in clinics. This work demonstrates for the first time that this hybrid nanofiber membrane scaffold can be utilized as a potential candidate for osteoarthritis treatment.


Assuntos
Cartilagem Articular/efeitos dos fármacos , Ácido Hialurônico/administração & dosagem , Nanofibras/administração & dosagem , Osteoartrite/terapia , Peptídeos/administração & dosagem , Animais , Cartilagem Articular/química , Sobrevivência Celular , Condrócitos/citologia , Cromatografia Líquida , Dicroísmo Circular , Membro Posterior/patologia , Inflamação , Masculino , Espectrometria de Massas , Células-Tronco Mesenquimais/metabolismo , Microscopia Eletrônica de Varredura , Microscopia Eletrônica de Transmissão , Oscilometria , Osteoartrite/fisiopatologia , Ratos , Ratos Sprague-Dawley , Reologia , Estresse Mecânico , Tecidos Suporte
11.
Turk J Phys Med Rehabil ; 64(1): 59-65, 2018 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31453490

RESUMO

Objectives: This study aims to evaluate the efficacy of prolotherapy injections for the treatment of plantar fasciitis. Material and methods: Between October 2014 and October 2015, 60 patients with symptomatic chronic plantar fasciitis were randomly divided into two groups, as control (n=31) and prolotherapy (n=29) groups. In the prolotherapy group, ultrasound-guided prolotherapy injections into the plantar fascia up to five different points were performed three times every 21 days. In the control group, the patients received instructions for plantar fascia and Achilles tendon stretching exercises three times a week for three months. Additionally, all patients were given heel lifts and instructed to refrain from heavy loading activity. The patients were evaluated via the Visual Analog Scale (VAS), Food and Ankle Outcome Score (FAOS), and Foot Function Index (FFI) at baseline and at 21, 42, 90, and 360 days during follow-up. Results: A total of 50 patients completed follow-up (26 patients in the prolotherapy group and 24 patients in the control group). The VAS, FAOS, and FFI scores were significantly improved in both groups (p<0.001). There were no statistically significant differences in the pain and functional scores at 21 days of treatment between the groups. The VAS and FAOS scores were higher in the prolotherapy group than the control group at 42, 90, and 360 days of treatment. The FFI scores were also higher in the prolotherapy group than the controls at 42 and 90 days of treatment; however, both groups had similar scores at 360 days. Conclusion: Our study results suggest that prolotherapy is an effective auxiliary method for treating chronic plantar fasciitis.

12.
J Int Adv Otol ; 13(3): 345-348, 2017 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-29165310

RESUMO

OBJECTIVE: To determine the serum iron (Fe), zinc (Zn), manganese (Mn), copper (Cu), magnesium (Mg), cobalt (Co), and lead (Pb) levels in patients with chronic otitis media (COM) and to evaluate the association of the serum levels of these elements with treatment outcomes. MATERIALS AND METHODS: Thirty-one healthy volunteers and 31 patients with COM were prospectively included in this study. Serum levels of Fe, Zn, Mn, Mg, Cu, Co, and Pb were determined by an atomic absorption UNICAM-929 spectrophotometer. RESULTS: Serum Co, Pb, and Fe levels were significantly increased (p<0.001) and serum Cu, Zn, Mg, and Mn levels were significantly reduced in patients with COM compared with controls (p<0.001). Serum Co and Mn levels were significantly decreased (p<0.001 and p<0.005, respectively) and serum Cu levels were significantly increased after surgery (p<0.005). The other evaluated blood chemicals and heavy metals did not exhibit significant differences (p>0.05). CONCLUSION: Significant alterations in the serum chemical composition of patients with COM were observed. Moreover, with surgical treatment, serum levels of some of these chemicals were significantly altered. Further prospective studies are warranted to elucidate the exact association of these alterations in the etiopathogenesis of COM.


Assuntos
Metais Pesados/metabolismo , Otite Média/metabolismo , Adulto , Doença Crônica , Feminino , Humanos , Masculino , Metais Pesados/sangue , Pessoa de Meia-Idade , Otite Média/cirurgia , Oligoelementos/sangue , Oligoelementos/metabolismo , Resultado do Tratamento
13.
IEEE Trans Image Process ; 26(11): 5270-5283, 2017 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-28767369

RESUMO

Correlation filters have been successfully used in visual tracking due to their modeling power and computational efficiency. However, the state-of-the-art correlation filter-based (CFB) tracking algorithms tend to quickly discard the previous poses of the target, since they consider only a single filter in their models. On the contrary, our approach is to register multiple CFB trackers for previous poses and exploit the registered knowledge when an appearance change occurs. To this end, we propose a novel tracking algorithm [of complexity O(D) ] based on a large ensemble of CFB trackers. The ensemble [of size O(2D) ] is organized over a binary tree (depth D ), and learns the target appearance subspaces such that each constituent tracker becomes an expert of a certain appearance. During tracking, the proposed algorithm combines only the appearance-aware relevant experts to produce boosted tracking decisions. Additionally, we propose a versatile spatial windowing technique to enhance the individual expert trackers. For this purpose, spatial windows are learned for target objects as well as the correlation filters and then the windowed regions are processed for more robust correlations. In our extensive experiments on benchmark datasets, we achieve a substantial performance increase by using the proposed tracking algorithm together with the spatial windowing.

14.
Acta Orthop Traumatol Turc ; 51(2): 155-159, 2017 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-28089510

RESUMO

OBJECTIVE: The aim of this study was to propose a new computer based method for measuring acetabular angles on hip radiographs and to assess its practicality, sensitivity and reliability for acetabular angle measurement. METHODS: A total of 314 acetabulum were assessed on 157 pelvic X-ray images. Acetabular angles were measured with both the conventional method (Method 1) and our proposed method (Method 2). All the Acetabular Index (AI) angle, Acetabular Angle (AA) and Acetabular Center (ACM) angle were measured with both methods. RESULTS: The mean AI angle for Method 1 is 11.02° ± 2.7° and the mean AI angle for Method 2 is 10.08° ± 1.88°, the mean AA angle for Method 1 is 39.5° ± 5.3° and the mean AA angle for Method 2 is 39.36° ± 4.68°, the mean ACM angle for Method 1 is 50.5° ± 6.01° and the mean ACM angle for Method 2 is 55.42° ± 12.43°. CONCLUSION: Our novel automated method appear to be reliable and practical for acetabular angle measurement on hip radiographs. LEVEL OF EVIDENCE: Level III, Diagnostic study.


Assuntos
Acetábulo/diagnóstico por imagem , Diagnóstico por Computador , Articulação do Quadril/diagnóstico por imagem , Radiografia/métodos , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Valores de Referência , Reprodutibilidade dos Testes , Estudos Retrospectivos
15.
Biomater Sci ; 4(9): 1328-39, 2016 Aug 16.
Artigo em Inglês | MEDLINE | ID: mdl-27447002

RESUMO

Biomineralization of the extracellular matrix (ECM) plays a crucial role in bone formation. Functional and structural biomimetic native bone ECM components can therefore be used to change the fate of stem cells and induce bone regeneration and mineralization. Glycosaminoglycan (GAG) mimetic peptide nanofibers can interact with several growth factors. These nanostructures are capable of enhancing the osteogenic activity and mineral deposition of osteoblastic cells, which is indicative of their potential application in bone tissue regeneration. In this study, we investigated the potential of GAG-mimetic peptide nanofibers to promote the osteogenic differentiation of rat mesenchymal stem cells (rMSCs) in vitro and enhance the bone regeneration and biomineralization process in vivo in a rabbit tibial bone defect model. Alkaline phosphatase (ALP) activity and Alizarin red staining results suggested that osteogenic differentiation is enhanced when rMSCs are cultured on GAG-mimetic peptide nanofibers. Moreover, osteogenic marker genes were shown to be upregulated in the presence of the peptide nanofiber system. Histological and micro-computed tomography (Micro-CT) observations of regenerated bone defects in rabbit tibia bone also suggested that the injection of a GAG-mimetic nanofiber gel supports cortical bone deposition by enhancing the secretion of an inorganic mineral matrix. The volume of the repaired cortical bone was higher in GAG-PA gel injected animals. The overall results indicate that GAG-mimetic peptide nanofibers can be utilized effectively as a new bioactive platform for bone regeneration.


Assuntos
Géis/química , Glicosaminoglicanos/química , Osteogênese/fisiologia , Peptídeos/química , Tecidos Suporte/química , Animais , Diferenciação Celular , Células Cultivadas , Células-Tronco Mesenquimais/citologia , Nanofibras/química , Coelhos , Ratos , Tecidos Suporte/normas , Microtomografia por Raio-X
16.
Biomacromolecules ; 17(2): 679-89, 2016 Feb 08.
Artigo em Inglês | MEDLINE | ID: mdl-26716910

RESUMO

Glycosaminoglycans (GAGs) and glycoproteins are vital components of the extracellular matrix, directing cell proliferation, differentiation, and migration and tissue homeostasis. Here, we demonstrate supramolecular GAG-like glycopeptide nanofibers mimicking bioactive functions of natural hyaluronic acid molecules. Self-assembly of the glycopeptide amphiphile molecules enable organization of glucose residues in close proximity on a nanoscale structure forming a supramolecular GAG-like system. Our in vitro culture results indicated that the glycopeptide nanofibers are recognized through CD44 receptors, and promote chondrogenic differentiation of mesenchymal stem cells. We analyzed the bioactivity of GAG-like glycopeptide nanofibers in chondrogenic differentiation and injury models because hyaluronic acid is a major component of articular cartilage. Capacity of glycopeptide nanofibers on in vivo cartilage regeneration was demonstrated in microfracture treated osteochondral defect healing. The glycopeptide nanofibers act as a cell-instructive synthetic counterpart of hyaluronic acid, and they can be used in stem cell-based cartilage regeneration therapies.


Assuntos
Cartilagem Articular/fisiologia , Glicopeptídeos/química , Nanofibras/química , Regeneração , Animais , Diferenciação Celular , Proliferação de Células , Células Cultivadas , Condrogênese , Receptores de Hialuronatos/metabolismo , Ácido Hialurônico/química , Masculino , Células-Tronco Mesenquimais/fisiologia , Camundongos , Mimetismo Molecular , Nanofibras/ultraestrutura , Coelhos , Espalhamento a Baixo Ângulo , Tecidos Suporte/química , Difração de Raios X
17.
Acta Orthop Traumatol Turc ; 49(5): 508-12, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26422346

RESUMO

OBJECTIVE: The aim of this study was to analyze the validity and reliability of the Turkish version (ICOAP-TR) of the intermittent and constant osteoarthritis pain (ICOAP) questionnaire in patients with knee osteoarthritis (OA). METHODS: Thirty-eight volunteer patients diagnosed with knee OA answered the questionnaire twice with an interval of 2-4 days. The reliability of the measurement was assessed using Cronbach's alpha coefficient and intraclass correlation (ICC) for test-retest reliability. Criterion validity was tested against the Western Ontario and McMaster Universities Arthritis Index (WOMAC) pain score and visual analog scale (VAS) designed to assess the perceived discomfort rated by the patient. RESULTS: Test-retest reliability was found to be ICC=0.942 for total score, 0.902 for constant pain subscale, and 0.945 for intermittent pain subscale. Internal consistency was tested using Cronbach's alpha and was found to be 0.970 for total score, 0.948 for constant pain subscale, and 0.972 for intermittent pain subscale. For criterion validity, the correlation between the total score of ICOAP-TR and WOMAC pain subscale was r=0.779 (p<0.05), and correlation between total score of ICOAP-TR and VAS was r=0.570 (p<0.05). CONCLUSION: The ICOAP-TR is a reliable and valid instrument to be used with patients with knee OA.


Assuntos
Articulação do Joelho/fisiopatologia , Osteoartrite do Joelho/diagnóstico , Medição da Dor/métodos , Dor/classificação , Inquéritos e Questionários/normas , Adulto , Feminino , Humanos , Idioma , Masculino , Pessoa de Meia-Idade , Reprodutibilidade dos Testes , Traduções , Turquia
18.
J Surg Res ; 196(2): 313-9, 2015 Jun 15.
Artigo em Inglês | MEDLINE | ID: mdl-25840486

RESUMO

BACKGROUND: Tourniquet-induced ischemia-reperfusion, which affects local and distant organs, is very common in orthopedic surgery. Hypothermia is used in traumatic tissue during ischemic period commonly. Ozone (O3) has been recommended as a novel therapeutic agent in various medical conditions. The objective of the study was to evaluate and compare the effect of hypothermia (H) and O3 on ischemia-reperfusion injury of skeletal muscle in rats by measuring oxidative parameters and inducible nitric oxide synthase (iNOS) levels. MATERIALS AND METHODS: Eighteen rats (Wistar albino) were separated into five groups randomly (sham, IR, IR + H, IR + O3, IR + H + O3; n = 6). The lower right extremity of all rats was subjected to 2 h of ischemia and 22 h of reperfusion clamping the common iliac artery and using the rubber-band technique at the level of the lesser trochanter under general anesthesia. Two hours of hypothermia were applied during the first 2 h of reperfusion in two groups. O3 was applied in two groups. All rats were sacrificed after the IR period with high dose of anesthesia. The tibialis anterior muscle and blood were saved. Levels of superoxide dismutase, glutathione peroxidase, MDA, NOx, and interleukin-1ß were measured in the muscle. Creatinine kinase, lactate dehydrogenase, aspartate aminotransferase, urea, creatinine, and electrolytes were measured in serum. Immunohistochemical iNOS staining was performed on muscle samples. RESULTS: The levels of MDA, NOx, and interleukin-1ß in muscle were raised in the IR group compared with those in the sham group. The same parameters were lower in the groups of IR + H, IR + O3, and IR + H + O3 compared with those in the IR group. Superoxide dismutase and glutathione peroxidase activities in muscle were lower in the IR group compared with those in the sham group; however, same parameters were higher in the groups of IR + H, IR + O3, and IR + H + O3 compared with those in the IR group. Score and intensity of iNOS staining in skeletal muscle in the IR group was increased compared with that in the sham group and decreased in the groups of IR + H, IR + O3, and IR + H + O3 compared with that in the IR group. Levels of creatinine kinase, aspartate aminotransferase, and K in the three treatment groups decreased compared with those in the IR group. CONCLUSIONS: These findings showed that hypothermia, which has more affect, and O3 decreased the tourniquet-induced IR injury in the rat's muscle-skeletal system by reducing the levels of oxidative and nitrosative stress parameters and enhancing antioxidant enzymes. Hypothermia and O3 had no synergistic effect. Hypothermic reperfusion and O3 preconditioning might be beneficial in skeletal muscle IR injury-associated tourniquet.


Assuntos
Hipotermia Induzida , Músculo Esquelético/irrigação sanguínea , Estresse Oxidativo , Ozônio/uso terapêutico , Traumatismo por Reperfusão/terapia , Animais , Masculino , Distribuição Aleatória , Ratos Wistar
19.
IEEE Trans Neural Netw Learn Syst ; 26(10): 2381-95, 2015 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-25608311

RESUMO

We introduce a comprehensive and statistical framework in a model free setting for a complete treatment of localized data corruptions due to severe noise sources, e.g., an occluder in the case of a visual recording. Within this framework, we propose: 1) a novel algorithm to efficiently separate, i.e., detect and localize, possible corruptions from a given suspicious data instance and 2) a maximum a posteriori estimator to impute the corrupted data. As a generalization to Euclidean distance, we also propose a novel distance measure, which is based on the ranked deviations among the data attributes and empirically shown to be superior in separating the corruptions. Our algorithm first splits the suspicious instance into parts through a binary partitioning tree in the space of data attributes and iteratively tests those parts to detect local anomalies using the nominal statistics extracted from an uncorrupted (clean) reference data set. Once each part is labeled as anomalous versus normal, the corresponding binary patterns over this tree that characterize corruptions are identified and the affected attributes are imputed. Under a certain conditional independency structure assumed for the binary patterns, we analytically show that the false alarm rate of the introduced algorithm in detecting the corruptions is independent of the data and can be directly set without any parameter tuning. The proposed framework is tested over several well-known machine learning data sets with synthetically generated corruptions and experimentally shown to produce remarkable improvements in terms of classification purposes with strong corruption separation capabilities. Our experiments also indicate that the proposed algorithms outperform the typical approaches and are robust to varying training phase conditions.

20.
Asian Nurs Res (Korean Soc Nurs Sci) ; 9(4): 278-84, 2015 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-26724235

RESUMO

PURPOSE: The purpose of this study was to determine the validity and reliability of the Turkish version of the Immobilization Comfort Questionnaire (ICQ). METHODS: The sample used in this methodological study consisted of 121 patients undergoing lower extremity arthroscopy in a training and research hospital. The validity study of the questionnaire assessed language validity, structural validity and criterion validity. Structural validity was evaluated via exploratory factor analysis. Criterion validity was evaluated by assessing the correlation between the visual analog scale (VAS) scores (i.e., the comfort and pain VAS scores) and the ICQ scores using Spearman's correlation test. The Kaiser-Meyer-Olkin coefficient and Bartlett's test of sphericity were used to determine the suitability of the data for factor analysis. Internal consistency was evaluated to determine reliability. The data were analyzed with SPSS version 15.00 for Windows. Descriptive statistics were presented as frequencies, percentages, means and standard deviations. A p value ≤ .05 was considered statistically significant. RESULTS: A moderate positive correlation was found between the ICQ scores and the VAS comfort scores; a moderate negative correlation was found between the ICQ and the VAS pain measures in the criterion validity analysis. Cronbach α values of .75 and .82 were found for the first and second measurements, respectively. CONCLUSIONS: The findings of this study reveal that the ICQ is a valid and reliable tool for assessing the comfort of patients in Turkey who are immobilized because of lower extremity orthopedic problems.


Assuntos
Artroscopia , Extremidade Inferior/cirurgia , Medição da Dor/métodos , Qualidade de Vida/psicologia , Adolescente , Adulto , Idoso , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Reprodutibilidade dos Testes , Inquéritos e Questionários , Traduções , Turquia , Adulto Jovem
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