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1.
Int J Biol Macromol ; 216: 799-809, 2022 Jul 30.
Artigo em Inglês | MEDLINE | ID: mdl-35917850

RESUMO

Food-borne carbon dots (FCDs) produced naturally during food thermal processing are one of important factors affecting human health. The FCDs will inevitably encounter blood proteins after oral administration and spontaneously form protein coronas. In this study, the interaction of three major blood proteins, including albumin, gamma globulin, and fibrinogen, with FCDs from roasted mackerel was investigated for the first time. The purpose of the research is to explore the effect of the protein corona on the biological effects of cytotoxicity and the metabolic response. The results showed that FCDs spontaneously bound to the three blood proteins, and the process involved the participation of multiple interaction forces. Three protein coronas attenuated FCD-mediated cell viability damage, oxidative stress, and mitochondrial membrane potential. Further metabolomics analysis showed that FCDs disrupted cellular carbohydrate, amino acid, and nucleotide metabolism and significantly affected the expression of six metabolic pathways in normal rat kidney cells. The protein corona alleviated the disorder of energy and substance metabolism pathways. However, the protein corona inevitably expands the range of affected metabolic responses. The results of this study are of great value in exploring the toxicity characteristics of FCDs and their protein coronas.

2.
J Endocrinol Invest ; 2022 Jul 14.
Artigo em Inglês | MEDLINE | ID: mdl-35834165

RESUMO

OBJECTIVE: Insulin resistance develops due to skeletal muscle inflammation and endoplasmic reticulum (ER) stress. Stachydrine (STA), extracted from Leonurus heterophyllus, has been shown to suppress proliferation and induce apoptosis in breast cancer cells and exert anti-inflammatory properties in the brain, heart, and liver. However, the roles of STA in insulin signaling in skeletal muscle remain unclear. Herein, we examined the impacts of STA on insulin signaling in skeletal muscle under hyperlipidemic conditions and its related molecular mechanisms. METHODS: Various protein expression levels were determined by Western blotting. Levels of mouse serum cytokines were measured by ELISA. RESULTS: We found that STA-ameliorated inflammation and ER stress, leading to attenuation of insulin resistance in palmitate-treated C2C12 myocytes. STA dose-dependently enhanced AMPK phosphorylation and HO-1 expression. Administration of STA attenuated not only insulin resistance but also inflammation and ER stress in the skeletal muscle of high-fat diet (HFD)-fed mice. Additionally, STA-ameliorated glucose tolerance and insulin sensitivity, as well as serum TNFα and MCP-1, in mice fed a HFD. Small interfering (si) RNA-associated suppression of AMPK or HO-1 expression abolished the effects of STA in C2C12 myocytes. CONCLUSIONS: These results suggest that STA activates AMPK/HO-1 signaling, resulting in reduced inflammation and ER stress, thereby improving skeletal muscle insulin resistance. Using STA as a natural ingredient, this research successfully treated insulin resistance and type 2 diabetes.

3.
Front Chem ; 10: 894759, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35864869

RESUMO

Graphene and its derivatives have been a burning issue in the last 10 years. Although many reviews described its application in electrochemical detection, few were focused on food detection. Herein, we reviewed the recent progress in applying graphene and composite materials in food detection during the past 10 years. We pay attention to food coloring materials, pesticides, antibiotics, heavy metal ion residues, and other common hazards. The advantages of graphene composites in electrochemical detection are described in detail. The differences between electrochemical detection involving graphene and traditional inherent food detection are analyzed and compared in depth. The results proved that electrochemical food detection based on graphene composites is more beneficial. The current defects and deficiencies in graphene composite modified electrode development are discussed, and the application prospects and direction of graphene in future food detection are forecasted.

4.
Molecules ; 27(13)2022 Jul 03.
Artigo em Inglês | MEDLINE | ID: mdl-35807524

RESUMO

We aimed to evaluate the inhibitory effect and mechanism of plantaricin YKX on S. aureus. The mode of action of plantaricin YKX against the cells of S. aureus indicated that plantaricin YKX was able to cause the leakage of cellular content and damage the structure of the cell membranes. Additionally, plantaricin YKX was also able to inhibit the formation of S. aureus biofilms. As the concentration of plantaricin YKX reached 3/4 MIC, the percentage of biofilm formation inhibition was over 50%. Fluorescent dye labeling combined with fluorescence microscopy confirmed the results. Finally, the effect of plantaricin YKX on the AI-2/LuxS QS system was investigated. Molecular docking predicted that the binding energy of AI-2 and plantaricin YKX was -4.7 kcal/mol and the binding energy of bacteriocin and luxS protein was -183.701 kcal/mol. The expression of the luxS gene increased significantly after being cocultured with plantaricin YKX, suggesting that plantaricin YKX can affect the QS system of S. aureus.


Assuntos
Bacteriocinas , Infecções Estafilocócicas , Staphylococcus aureus , Antibacterianos/química , Bacteriocinas/química , Bacteriocinas/farmacologia , Biofilmes/efeitos dos fármacos , Humanos , Simulação de Acoplamento Molecular , Infecções Estafilocócicas/tratamento farmacológico , Staphylococcus aureus/efeitos dos fármacos
5.
Crit Rev Food Sci Nutr ; : 1-19, 2022 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-35648034

RESUMO

Sample pretreatment is essential for trace analysis of pesticides in complex food and environment matrices. Recently, organic-inorganic hybrid materials have gained increasing attention in pesticide extraction and preconcentration. This review highlighted the common organic-inorganic hybrid materials used as absorbents in sample pretreatment for pesticide detection. Furthermore, the preparation and characterization of organic-inorganic hybrid materials were summarized. To obtain a deep understanding of adsorption toward target analytes, the adsorption mechanism and absorption evaluation were discussed. Finally, the applications of organic-inorganic hybrid materials in sample pretreatment techniques and perspectives in the future are also discussed.

6.
Foods ; 11(12)2022 Jun 13.
Artigo em Inglês | MEDLINE | ID: mdl-35741925

RESUMO

This study provides the first design and synthetic protocol for preparing highly sensitive and specific atrazine (ATR) monoclonal antibodies (mAbs). In this work, a previously unreported hapten, 2-chloro-4-ethylamino-6-isopropylamino-1,3,5-triazine, was designed and synthesized, which maximally exposed the characteristic amino group ATR to an animal immune system to induce the expected antibody. The molecular weight of the ATR hapten was 259.69 Da, and its purity was 97.8%. The properties of the anti-ATR mAb were systematically characterized. One 9F5 mAb, which can detect ATR, was obtained with an IC50 value (the concentration of analyte that produced 50% inhibition of ATR) of 1.678 µg/L for ATR. The molecular weight for the purified 9F5 mAb was approximately 52 kDa for the heavy chain and 15 kDa for the light chain. The anti-ATR mAb prepared in this study was the IgG1 type. The working range of the standard curve (IC20 (the concentration of analyte that produced 20% inhibition of ATR)-IC80 (the concentration of analyte that produced 80% inhibition of ATR)) was 0.384 to 11.565 µg/L. The prepared anti-ATR mAb had high specificity, sensitivity, and affinity with low cross-reactivity. The prepared anti-ATR mAb could provide the core raw material for establishing an ATR immunoassay.

7.
Biosensors (Basel) ; 12(6)2022 Jun 10.
Artigo em Inglês | MEDLINE | ID: mdl-35735550

RESUMO

The presence of pyrethroids in food and the environment due to their excessive use and extensive application in the agriculture industry represents a significant threat to public health. Therefore, the determination of the presence of pyrethroids in foods by simple, rapid, and sensitive methods is warranted. Herein, recognition methods for pyrethroids based on electrochemical and optical biosensors from the last five years are reviewed, including surface-enhanced Raman scattering (SERS), surface plasmon resonance (SPR), chemiluminescence, biochemical, fluorescence, and colorimetric methods. In addition, recognition elements used for pyrethroid detection, including enzymes, antigens/antibodies, aptamers, and molecular-imprinted polymers, are classified and discussed based on the bioreceptor types. The current research status, the advantages and disadvantages of existing methods, and future development trends are discussed. The research progress of rapid pyrethroid detection in our laboratory is also presented.


Assuntos
Técnicas Biossensoriais , Piretrinas , Técnicas Biossensoriais/métodos , Colorimetria , Análise Espectral Raman/métodos , Ressonância de Plasmônio de Superfície/métodos
8.
J Ginseng Res ; 46(3): 444-453, 2022 May.
Artigo em Inglês | MEDLINE | ID: mdl-35600773

RESUMO

Background: Compound K (CK) is among the protopanaxadiol (PPD)-type ginsenoside group, which produces multiple pharmacological effects. Herein, we examined the effects of CK on muscle atrophy under hyperlipidemic conditions along with its pro-myogenic effects. Further, the molecular pathways underlying the effects of CK on skeletal muscle have been justified. Methods: C2C12 myotubes were treated with palmitate and CK. C2C12 myoblasts were differentiated using CK for 4-5 days. For the in vivo experiments, CK was administered to mice fed on a high-fat diet for 8 weeks. The protein expression levels were analyzed using western blotting analysis. Target protein suppression was performed using small interfering (si) RNA transfection. Histological examination was performed using Jenner-Giemsa and H&E staining techniques. Results: CK treatment attenuated ER stress markers, such as eIF2α phosphorylation and CHOP expression and impaired myotube formation in palmitate-treated C2C12 myotubes and skeletal muscle of mice fed on HFD. CK treatment augmented AMPK along with autophagy markers in skeletal muscle cells in vitro and in vivo experiments. AMPK siRNA or 3-MA, an autophagy inhibitor, abrogated the impacts of CK in C2C12 myotubes. CK treatment augmented p38 and Akt phosphorylation, leading to an enhancement of C2C12 myogenesis. However, AMPK siRNA abolished the effects of CK in C2C12 myoblasts. Conclusion: These findings denote that CK prevents lipid-induced skeletal muscle apoptosis via AMPK/autophagy-mediated attenuation of ER stress and induction of myoblast differentiation. Therefore, we may suggest the use of CK as a potential therapeutic approach for treating muscle-wasting conditions associated with obesity.

9.
J Adv Res ; 37: 61-74, 2022 03.
Artigo em Inglês | MEDLINE | ID: mdl-35499055

RESUMO

Background: Organophosphorus pesticides (OPs), as insecticides or acaricides, are widely used in agricultural products to ensure agricultural production. However, widespread use of OPs leads to environmental contamination and significant negative consequences on biodiversity, food security, and water resources. Therefore, developing a sensitive and rapid method to determine OPs residues in different matrices is necessary. Originally, the enzyme inhibition methods are often used as preliminary screens of OPs in crops. Many studies on the characteristic of Au nanomaterials have constantly been emerging in the past decade. Combined with anisotropic Au nanomaterials, enzyme inhibition methods have the advantages of high sensitivity, durability, and high stability. Aim of Review: This review aims to summarize the principles and strategies of gold (Au) nanomaterials in enzyme inhibition methods, including colorimetric (dispersion, particle size of Au nanomaterials) and fluorometric (fluorescence energy transfer, internal filtration effect) detection, and electrochemical sensing system (shape of Au nanomaterials, Au nanomaterials combined with other nanomaterials). The application of enzyme inhibition in agricultural products and research progress was also outlined. Next, this review illustrates the advantages of Au nanomaterial-based enzyme inhibition methods compared with conventional enzyme inhibition methods. The detection limits and linear range of colorimetric and fluorometric detection and electrochemical biosensors have also been provided. At last, key perspectives, trends, gaps, and future research directions are proposed. Key Scientific Concepts of Review: Herein, we introduced the technology of enzyme inhibition method based on Au nanomaterials for onsite and infield rapid detection of organophosphorus pesticide.


Assuntos
Técnicas Biossensoriais , Nanoestruturas , Praguicidas , Agricultura , Técnicas Biossensoriais/métodos , Compostos Organofosforados , Praguicidas/análise
11.
Front Nutr ; 9: 882742, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35634401

RESUMO

In this study, we prepared cassava starch-based films by the casting method. Afterwards, the effects of geranium essential oil (GEO) on the prepared films' physicochemical, morphology, and antibacterial properties were revealed. We found that the thickness and elongation at the break of cassava starch films increased with increasing GEO concentration (from 0.5, 1, and 2%). However, increasing GEO concentration decreased the water content, water vapor permeability, and tensile strength of the prepared films'. Further, the addition of GEO increased the surface roughness, opacity, and antibacterial properties of the prepared films. With the increase of GEO concentration, L * and a * of cassava starch film decreased, while b * and Δ E increased. This study provides a theory for cassava starch-based films as a biological packaging material.

12.
Int Immunopharmacol ; 109: 108826, 2022 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-35569308

RESUMO

Chitosan oligosaccharide (COS) plays a vital role in improving the host system and mucosal immune function. So far, the impact of COS on mucosal immune response in the early stage of oral administration is not well understood. Herein, the distribution of COS after oral gavage and the protein expression changes related to innate immune by tandem mass tag (TMT)-based proteomic analysis were investigated. The results revealed that COS was mainly distributed in the stomach, duodenum, and kidney and increased the number of monocytes and lymphocytes in peripheral blood. A total of 21,677 proteins and 7,483 protein groups were identified. Among them, 338 significant differentially expressed proteins were screened, including 205 upregulated and 133 downregulated. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that the intestinal immune network for the IgA production pathway was activated, pIgR, MHCI, MHCII, Itgb2, Itgb7, and B2m were significantly increased (P < 0.05). Furthermore, the expression of the above molecular genes was detected by enzyme-linked immunosorbent assay (ELISA), western blotting, and quantitative real-time PCR. We found that the expressions of IgA, MHCII, TGF-ß1, IL-6, and pIgR were significantly increased (P < 0.05) 1 h after exposure to COS. The protein and mRNA expression of pIgR and MHCI were significantly increased (P < 0.05) at 0.5 h, while the AID protein level was significantly increased (P < 0.05) 1.5 h after COS exposure. The expression of MHCII and H2-Q10 was significantly increased (P < 0.05) by 1 h and 2 h post-exposure to COS. In conclusion, oral administration of COS can significantly enhance intestinal mucosal immunity in mice by activating the SIgA secretion pathway. These results suggest that COS can be used as an oral vaccine or drug adjuvant for small intestinal mucosa.


Assuntos
Quitosana , Imunoglobulina A Secretora , Animais , Imunidade nas Mucosas , Mucosa Intestinal , Intestino Delgado/metabolismo , Camundongos , Oligossacarídeos , Proteômica
13.
Front Nutr ; 9: 882682, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35548578

RESUMO

The demand for biodegradable products has increased; hence, a suitable method for producing green composites is essential. This study prepared corn starch-based films using the solution casting method, and the physicochemical properties of the prepared films were investigated using a mixture of glycerol (GLY) and erythritol (ERY) at different ratios (4:0, 3:1, 2:2, 1:3, and 0:4) as plasticizing agents. The crystallinity, hydrophilicity, mechanical properties, oxygen and water vapor, surface roughness, and thermal stability of corn starch-based films were analyzed using small-angle X-ray diffraction, water contact angle, automatic tensile testing machine, oxygen permeability tester and water vapor permeability analyzer, atomic force microscope, and thermogravimetric analyzer. With the increase in GLY ratio, the thickness, water-solubility, water content, water vapor permeability, elongation at break, oxygen permeability and V-shaped crystallization of the corn starch-based films increased. The tensile strength and the thermal stability decreased with increasing the GLY ratio. We developed a new plasticizer using glycerol and erythritol to improve the properties of starch films and provided the basis for the industrial production of corn starch-based films.

14.
Foods ; 11(7)2022 03 22.
Artigo em Inglês | MEDLINE | ID: mdl-35406986

RESUMO

An analytical method was developed to simultaneously determine pyridate, quizalofop-ethyl, and cyhalofop-butyl in brown rice, soybean, potato, pepper, and mandarin using LC-MS/MS. Purification was optimized using various sorbents: primary-secondary amine, octadecyl (C18) silica gel, graphitized carbon black, zirconium dioxide-modified silica particles, zirconium dioxide-modified silica particles (Z-SEP), and multi-walled carbon nanotubes (MWCNTs). Three versions of QuECHERS methods were then tested using the optimal purification agent. Finally, samples were extracted using acetonitrile and QuEChERS EN salts and purified using the Z-SEP sorbent. A six-point matrix-matched external calibration curve was constructed for the analytes. Good linearity was achieved with a determination coefficient ≥0.999. The limits of detection and quantification were 0.0075 mg/kg and 0.01 mg/kg, respectively. The method was validated after fortifying the target standards to the blank matrices at three concentration levels with five replicates for each concentration. The average recovery was within an acceptable range (70-120%), with a relative standard deviation <20%. The applicability of the developed method was evaluated with real-world market samples, all of which tested negative for these three herbicide residues. Therefore, this method can be used for the routine analysis of pyridate, quizalofop-ethyl, and cyhalofop-butyl in agricultural products.

15.
Biochem Biophys Res Commun ; 608: 142-148, 2022 06 11.
Artigo em Inglês | MEDLINE | ID: mdl-35398611

RESUMO

Abietic acid (AA), the main component of pine resin that has been traditionally used as Asian medicine, has been reported to demonstrate anti-inflammatory activities. Despite this, little is known about the effects of AA on hepatic endoplasmic reticulum (ER) stress and lipid metabolism. This study investigated the impacts of AA on ER stress and steatosis in in vitro obesity models. We found that Treatment with AA reduced lipid deposition and lipogenesis-related proteins expression in human primary hepatocytes. Augmented expression of ER stress markers (phospho-eukaryotic initiation factor-2α (eIF2α) and C/EBP homologous protein (CHOP)) in palmitate-treated hepatocytes were reversed by AA treatment. Further, AA treatment increased the expression of phospho-AMPK and oxygen-regulated protein 150 (ORP150) in hepatocytes. siRNA-associated knockdown of AMPK or ORP150 expression reduced the effects of AA on not only hepatic ER stress but also lipogenesis and apoptosis. These results denote that AA attenuates lipid accumulation in hepatocytes in the presence of palmitate through the suppression of ER stress by AMPK/ORP150 signaling. AA could be a potential candidate for treating non-alcoholic fatty liver disease.


Assuntos
Proteínas Quinases Ativadas por AMP , Abietanos , Estresse do Retículo Endoplasmático , Proteínas de Choque Térmico HSP70 , Hepatócitos , Proteínas Quinases Ativadas por AMP/metabolismo , Abietanos/farmacologia , Estresse do Retículo Endoplasmático/efeitos dos fármacos , Proteínas de Choque Térmico HSP70/metabolismo , Hepatócitos/metabolismo , Humanos , Hipercolesterolemia/metabolismo , Metabolismo dos Lipídeos , Fígado/metabolismo , Hepatopatia Gordurosa não Alcoólica/tratamento farmacológico , Hepatopatia Gordurosa não Alcoólica/metabolismo , Oxigênio/metabolismo , Palmitatos/metabolismo , Palmitatos/farmacologia
16.
Biomed J ; 2022 Apr 25.
Artigo em Inglês | MEDLINE | ID: mdl-35483573

RESUMO

BACKGROUND: Capmatinib (CAP) is a drug that has been used to treat non-small cell lung cancer (NSCLC) in adults. Presently, its novel effects on skeletal muscle insulin signaling, inflammation, and lipogenesis in adipocytes have been uncovered with a perspective of drug repositioning. However, the impact of CAP on LPS-mediated adhesion between human umbilical vein endothelial cells (HUVECs) and THP-1 monocytes has yet to be investigated. METHODS: HUVECs and THP-1 monocytes were treated with LPS and CAP. The protein expression levels were determined using Western blotting. Target protein knockdown was conducted using small interfering (si) RNA transfection. Adhesion between HUVECs and THP-1 cells was assayed using green fluorescent dye. RESULTS: This study found that CAP treatment ameliorated cell adhesion between THP-1 monocytes and HUVECs and the expression of adhesive molecules, such as intracellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and E-selectin. Moreover, phosphorylation of inflammatory markers, such as NFκB and IκB as well as TNFα and monocyte chemoattractant protein-1 (MCP-1) released from HUVECs and THP-1 monocytes, was prevented by CAP treatment. Treatment with CAP augmented PPARα and IL-10 expression. siRNA-associated suppression of PPARδ and IL-10 attenuated the effects of CAP on cell adhesion between HUVECs and THP-1 cells and inflammatory responses. Further, PPARα siRNA mitigated CAP-mediated induction of IL-10 expression. CONCLUSION: These findings imply that CAP improves inflamed endothelial-monocyte adhesion via a PPAR/IL-10-dependent pathway. The current study provides in vitro evidence for a therapeutic approach for treating atherosclerosis.

17.
Food Funct ; 13(9): 5416-5429, 2022 May 10.
Artigo em Inglês | MEDLINE | ID: mdl-35475434

RESUMO

Herein, we assessed the effects of Lycium barbarum oligosaccharides (LBO) on the intestinal microenvironment of a type 2 diabetes (T2D) mouse model through gut microbiome and metabolomics analysis. We set high (300 mg kg-1), medium (200 mg kg-1), and low (100 mg kg-1) doses of LBO for intervention once a day for 4 weeks. The results showed that the intervention effect of the medium-dose group was the most significant. It reduced the symptoms of hyperglycemia, inflammation, insulin resistance, and lipid accumulation in the T2D mouse model. It restored the structure of damaged tissues and cells, such as the pancreas, liver, and kidneys. LBO increased the relative abundance of beneficial bacteria, such as Lactobacillus, Bacteroides, Prevotella, and Akkermansia, and maintained intestinal barrier integrity. The faecal metabolic map showed that the contents of glycogen amino acids, such as proline, serine, and leucine, increased. The contents of cholic, capric, and dodecanoic acid decreased. In summary, we may suggest that LBO can be used as a prebiotic for treating T2D.


Assuntos
Diabetes Mellitus Tipo 2 , Microbioma Gastrointestinal , Lycium , Animais , Biologia Computacional , Diabetes Mellitus Tipo 2/tratamento farmacológico , Diabetes Mellitus Tipo 2/microbiologia , Glicolipídeos/farmacologia , Lycium/química , Metabolômica , Camundongos , Oligossacarídeos/farmacologia
18.
Molecules ; 27(7)2022 Mar 23.
Artigo em Inglês | MEDLINE | ID: mdl-35408465

RESUMO

Lactic acid bacteria (LAB) produce antimicrobial substances that could potentially inhibit the growth of pathogenic and food spoilage microorganisms. Lacticaseibacillus rhamnosus XN2, isolated from yak yoghurt, demonstrated antibacterial activity against Bacillus subtilis, B. cereus, Micrococcus luteus, Brochothrix thermosphacta, Clostridium butyricum, S. aureus, Listeria innocua CICC 10416, L. monocytogenes, and Escherichia coli. The antibacterial activity was estimated to be 3200 AU/mL after 30 h cultivation. Time-kill kinetics curve showed that the semi-purified cell-free supernatants (CFS) of strain XN2 possessed bactericidal activity. Flow cytometry analysis indicated disruption of the sensitive bacteria membrane by semi-purified CFS, which ultimately caused cell death. Interestingly, sub-lethal concentrations of semi-purified CFS were observed to reduce the production of α-haemolysin and biofilm formation. We further investigated the changes in the transcriptional level of luxS gene, which encodes signal molecule synthase (Al-2) induced by semi-purified CFS from strain XN2. In conclusion, L. rhamnosus XN2 and its bacteriocin showed antagonistic activity at both cellular and quorum sensing (QS) levels. Finally, bacteriocin was further purified by reversed-phase high-performance liquid chromatography (RP-HPLC), named bacteriocin XN2. The amino acid sequence was Met-Lue-Lys-Lys-Phe-Ser-Thr-Ala-Tyr-Val.


Assuntos
Bacteriocinas , Síndrome de Fadiga Crônica , Lactobacillus rhamnosus , Animais , Antibacterianos , Bovinos , Staphylococcus aureus , Iogurte
19.
Biochem Pharmacol ; 199: 115022, 2022 05.
Artigo em Inglês | MEDLINE | ID: mdl-35358477

RESUMO

Valdecoxib (VAL) is one of the non-steroidal anti-inflammatory drugs (NSAIDs) used to treat inflammatory disorders, such as rheumatoid arthritis, osteoarthritis, and menstrual cramps. Recently, VAL ameliorates skeletal muscle insulin resistance via suppression of inflammation. However, the effects of VAL on lipid metabolism in hepatocytes have not been seen yet. This study investigated the effects of VAL on lipid accumulation and lipogenesis in human primary hepatocytes. Treatment with VAL suppressed lipid accumulation and expressions of lipogenic genes, such as processed sterol regulatory element binding proteins (SREBP1) and stearoyl-CoA desaturase-1 (SCD1) in palmitate-treated hepatocytes. Furthermore, VAL ameliorated dose-dependently palmitate-induced ER stress markers. Treatment of hepatocytes with VAL increased AMPK phosphorylation and SIRT6 expression. siRNA-mediated suppression of AMPK or SIRT6 abolished the effects of VAL on lipid accumulation, lipogenesis, and endoplasmic reticulum (ER) stress in palmitate-treated hepatocytes. Administration of VAL ameliorated hepatic lipid accumulation and lipogenic protein expression in HFD-fed mice. Moreover, in vivo AMPK siRNA transfection abolished the effects of VAL on hepatic steatosis and lipid metabolism. These results suggest that VAL suppresses ER stress through the AMPK/SIRT6 pathway, thereby attenuating hepatic steatosis under hyperlipidemic conditions. Using VAL, the current study results provide clues for developing a novel therapeutic agent for treating non-alcoholic fatty liver disease.


Assuntos
Hepatopatia Gordurosa não Alcoólica , Sirtuínas , Proteínas Quinases Ativadas por AMP/metabolismo , Animais , Autofagia , Estresse do Retículo Endoplasmático , Hepatócitos/metabolismo , Isoxazóis , Metabolismo dos Lipídeos , Fígado/metabolismo , Camundongos , Camundongos Endogâmicos C57BL , Hepatopatia Gordurosa não Alcoólica/metabolismo , Palmitatos/metabolismo , Palmitatos/farmacologia , Palmitatos/uso terapêutico , RNA Interferente Pequeno/metabolismo , Sirtuínas/metabolismo , Sirtuínas/farmacologia , Sirtuínas/uso terapêutico , Sulfonamidas
20.
Front Nutr ; 9: 846333, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35284432

RESUMO

The demand for Chinese chives is growing as they are also rich in vitamins, fiber, and sulfur nutrients. Chinese chives should be sprayed with imidacloprid to control pests and diseases to safeguard their yield and to meet the demands of East Asian consumers for Chinese chives. Overspraying of imidacloprid can lead to residues in Chinese chives, posing a severe risk to human health. To reduce the harmful effects of imidacloprid residues on humans, we investigated the imidacloprid dissipation pattern and the final residue on Chinese chives using the quick, easy, cheap, effective, rugged, and safe (QuEChERS) method combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS). Good linearity (R 2= 0.9988), accuracy (expressed as recovery % of 78.34-91.17%), precision [expressed as relative SDs (RSDs) of 0.48-6.43%], and sensitivity [a limit of quantification (LOQ) ≤ 8.07 × 104 mg/kg] were achieved. The dissipation dynamics were consistent with the first-order kinetics, with a half-life of 2.92 days. The final residual levels on Chinese chives were 0.00923-0.166 mg/kg, which is lower than the maximum residue limits (MRLs) of 1 mg/kg for imidacloprid on Chinese chives. A risk assessment index of <1 indicates that Chinese chives are safe for consumption.

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