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1.
Shock ; 2020 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-31917735

RESUMO

INTRODUCTION: In human sepsis, little is known about the relationships between complement activation and the clinical characteristics of sepsis, including disseminated intravascular coagulation (DIC), interventions, and prognosis. MATERIALS AND METHODS: Adult patients with sepsis admitted from November 2016 to December 2018 were included. We used the plasma levels of soluble C5b-9 (SC5b-9) as a marker of complement activation. We compared the clinical characteristics and complement components between patients with and without DIC. We also compared the clinical characteristics and each DIC parameter across quartile groups for the SC5b-9 value. RESULTS: Forty-nine sepsis patients were eligible. Thirty-four patients developed DIC, and eight patients died. The median (interquartile range) SC5b-9 value was 342 (261-501) ng/mL. Compared with patients without DIC, patients with DIC showed lower C3 levels (mean, 95.7 vs. 70.4 mg/dL, P < 0.01) and higher SC5b-9 levels (median, 287 vs. 400 ng/mL, P = 0.01). Patients were stratified by SC5b-9 quartile (ng/mL: low: < 260, moderate: 260-342, high: 343-501, highest: > 501). The mean Sequential Organ Failure Assessment score varied across these groups (P = 0.02). In the high and highest groups, many more patients received vasopressors and developed DIC. In the highest group, the coagulation parameters were severe, and thrombocytopenia was prolonged. In-hospital mortality tended to be high (33%) in the highest group. CONCLUSIONS: The degree of complement activation is related to DIC, severity, intensive interventions, and mortality. Further studies are needed to confirm the usefulness of SC5b-9 for stratifying sepsis patients.

2.
Inorg Chem ; 58(22): 15410-15416, 2019 Nov 18.
Artigo em Inglês | MEDLINE | ID: mdl-31692349

RESUMO

Structural phase transitions of calcium strontium sulfoaluminate series, (Ca1-xSrx)8[AlO2]12(SO4)2 ((CS)AS-x) with x = 0.80-1.00, are systematically investigated by powder X-ray diffraction, dielectric measurements, and pyroelectric measurements, to clarify a phase diagram of (CS)AS-x (x = 0.80-1.00). A pure strontium sulfoaluminate, (CS)AS-1.00, is found to undergo three phase transitions, which take place successively on cooling from a prototypical cubic phase with the symmetry of Im3̅m. Though the room-temperature phase of (CS)AS-1.00 was previously reported to be of polar Pcc2, the pyroelectric measurements clarified a nonpolar character of the crystal symmetry. The dielectric measurements suggest a possibility of an antiferroelectric ground state of (CS)AS-x in the Sr-rich compositions. As x decreases, the ground state changes to a short-range-ordered state, implying a unique phase transition from the antiferroelectric state to the antiferroelectric-relaxor state. The present study provides an intriguing playground for designing new ferro/antiferroelectric materials.

3.
Inorg Chem ; 58(16): 10928-10935, 2019 Aug 19.
Artigo em Inglês | MEDLINE | ID: mdl-31393726

RESUMO

Layered double hydroxides (LDHs) are promising compounds in a wide range of fields. However, exchange of CO32- anions with other anions is necessary, because the CO32- anions are strongly affixed in the LDH interlayer space. To elucidate the reason for the extremely high stability of CO32- anions intercalated in LDHs, we investigated in detail the chemical states of CO32- anions and hydrated water molecules in the LDH interlayer space by synchrotron radiation X-ray diffraction, solid-state NMR spectroscopy, and Raman spectroscopy. We found the rigidity of the network structure formed between the CO32- anions, hydrated water molecules, and the hydroxyl groups on the metal hydroxide layer surface to be a crucial factor underlying the stability of CO32- anions in the LDH interlayer space.

4.
BMJ Open ; 9(8): e029186, 2019 08 20.
Artigo em Inglês | MEDLINE | ID: mdl-31434772

RESUMO

OBJECTIVES: Physician-staffed prehospital units are widely used in many countries. The criteria for predicting fatal injury are well recognised for trauma victims, but there are no criteria for predicting critical condition for non-trauma patients. This study aimed to identify the factors associated with non-trauma cases receiving prehospital interventions by physicians. DESIGN: Retrospective observational study. SETTING: Physician-staffed prehospital unit (car) at a single-base hospital in a suburban city in Japan. PARTICIPANTS: Participants were 1058 non-trauma patients who received prehospital medical examinations from April 2014 to December 2017. OUTCOME MEASURES: The outcome was the occurrence of physician-only interventions (POIs) exceeding paramedics' competencies. Univariate analysis and multiple logistic regression analysis were performed. Patient's age and gender, presumed disease category, type of location of the emergency, time of alarm, activation time, activator's occupation, time to arrival, transportation time and the destination facility were included as covariates. RESULTS: POIs were provided to 380 (36%) patients. Patient's age, presumed disease category, type of location of the emergency, activator's occupation, time to arrival, transportation time and the destination facility were identified as potential independent factors. Multiple logistic regression analysis found that patient's age, presumed disease category, type of location of the emergency, transportation time and destination facility were the significant independent factors. Transportation times of more than 15 min (adjusted ORs (AORs)=4.17, 95% CI 2.59 to 6.72, p<0.01) or 10 to 14 min (AOR=3.66, 95% CI 2.32 to 5.79, p<0.01) and patient age of 40-59 years (AOR=3.16, 95% CI 1.66 to 6.01, p<0.01) were the strongest independent factors. CONCLUSIONS: This study identified the factors associated with non-trauma cases receiving prehospital POIs. Patient's age, presumed disease category, type of location of the emergency and transportation time are independent factors associated with requiring POIs.

5.
Acute Med Surg ; 6(1): 49-53, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30651997

RESUMO

Aim: Athletes who suffer sport-related concussions but continue playing have a high probability of experiencing more severe symptoms with any subsequent concussion. This study used data from the authors' clinical department to retrospectively investigate the clinical characteristics of sport-related concussion and factors associated with delays in examinations. Methods: The study included 38 patients with sport-related concussions who were treated at the authors' hospital. The sports during which the injuries occurred, occasions of injury, factors affecting the time from injury until examination at hospital, and prognoses were evaluated retrospectively. Results: Sport-related concussions most frequently occurred in rugby and judo, where tackling, throwing, and other types of physical contact can result in impacts to the head region at high angular or linear velocity with acceleration. Some subjects showed consciousness disturbance and/or clear subjective symptoms immediately after the injury and were therefore examined at the hospital rapidly. However, other subjects who suffered amnesia or less obvious symptoms continued to play after the injury and had delayed examinations. In addition, there was a tendency for examinations to be delayed when an on-site physician was present. Conclusions: In contact sports where injury-resulting events occur, such as impacts to the head at high angular or linear velocity with acceleration, athletes should be immediately withdrawn from play and evaluated for concussion, even if there are no obvious head injuries or symptoms. The immediate ascertainment of symptoms after physical contact would improve the rapid detection of sport-related concussion.

6.
Dent Mater J ; 37(1): 152-156, 2018 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-28954942

RESUMO

The aim of this study was to investigate a failure criterion to predict flexural strengths of composite resins (CR) by three-dimensional finite element analysis (3D-FEA). Models of flexural strength for test specimens of CR and rods comprising a three-point loading were designed. Calculation of Young's moduli and Poisson's ratios of CR were conducted using a modified McGee-McCullough model. Using the experimental CR, flexural strengths were measured by three-point bending tests with crosshead speed 1.0 mm/min and compared with the values determined by in silico analysis. The flexural strengths of experimental CR calculated using the maximum principal strain significantly correlated with those obtained in silico amongst the four types of failure criteria applied. The in silico analytical model established in this study was found to be effective to predict the flexural strengths of CR incorporating various silica filler contents by maximum principal strain.


Assuntos
Resinas Compostas/química , Desenho Assistido por Computador , Materiais Dentários/química , Simulação por Computador , Análise do Estresse Dentário , Módulo de Elasticidade , Análise de Elementos Finitos , Teste de Materiais , Estresse Mecânico
7.
J Rural Med ; 12(1): 12-19, 2017 May.
Artigo em Inglês | MEDLINE | ID: mdl-28593012

RESUMO

Objective: Involvement of all regional medical facilities in a trauma system is challenging in rural regions. We hypothesized that the physician-staffed helicopter emergency medical service potentially encouraged local facilities to participate in trauma systems by providing the transport of patients with trauma to those facilities in a rural setting. Materials and Methods: We performed two retrospective observational studies. First, yearly changes in the numbers of patients with trauma and destination facilities were surveyed using records from the Miyazaki physician-staffed helicopter emergency medical service from April 2012 to March 2014. Second, we obtained data from medical records regarding the mechanism of injury, severity of injury, resuscitative interventions performed within 24 h after admission, secondary transports owing to undertriage by attending physicians, and deaths resulting from potentially preventable causes. Data from patients transported to the designated trauma center and those transported to non-designated trauma centers in Miyazaki were compared. Results: In total, 524 patients were included. The number of patients transported to non-designated trauma centers and the number of non-designated trauma centers receiving patients increased after the second year. We surveyed 469 patient medical records (90%). There were 194 patients with major injuries (41%) and 104 patients with multiple injuries (22%), and 185 patients (39%) received resuscitative interventions. The designated trauma centers received many more patients with trauma (366 vs. 103), including many more patients with major injuries (47% vs. 21%, p < 0.01) and multiple injuries (25% vs. 13%, p < 0.01), than the non-designated trauma centers. The number of patients with major injuries and patients who received resuscitative interventions increased for non-designated trauma centers after the second year. There were 9 secondary transports and 26 deaths. None of these secondary transports resulted from undertriage by staff physicians and none of these deaths resulted from potentially preventable causes. Conclusion: The rural physician-staffed helicopter emergency medical service potentially encouraged non-designated trauma centers to participate in trauma systems while maintaining patient safety.

8.
Comput Methods Biomech Biomed Engin ; 20(7): 714-719, 2017 May.
Artigo em Inglês | MEDLINE | ID: mdl-28387166

RESUMO

The objective of this study was to assess the effect of silica nano-filler particle diameters in a computer-aided design/manufacturing (CAD/CAM) composite resin (CR) block on physical properties at the multi-scale in silico. CAD/CAM CR blocks were modeled, consisting of silica nano-filler particles (20, 40, 60, 80, and 100 nm) and matrix (Bis-GMA/TEGDMA), with filler volume contents of 55.161%. Calculation of Young's moduli and Poisson's ratios for the block at macro-scale were analyzed by homogenization. Macro-scale CAD/CAM CR blocks (3 × 3 × 3 mm) were modeled and compressive strengths were defined when the fracture loads exceeded 6075 N. MPS values of the nano-scale models were compared by localization analysis. As the filler size decreased, Young's moduli and compressive strength increased, while Poisson's ratios and MPS decreased. All parameters were significantly correlated with the diameters of the filler particles (Pearson's correlation test, r = -0.949, 0.943, -0.951, 0.976, p < 0.05). The in silico multi-scale model established in this study demonstrates that the Young's moduli, Poisson's ratios, and compressive strengths of CAD/CAM CR blocks can be enhanced by loading silica nanofiller particles of smaller diameter. CAD/CAM CR blocks by using smaller silica nano-filler particles have a potential to increase fracture resistance.


Assuntos
Resinas Compostas/química , Desenho Assistido por Computador , Teste de Materiais , Nanopartículas/química , Tamanho da Partícula , Bis-Fenol A-Glicidil Metacrilato/química , Força Compressiva , Módulo de Elasticidade , Polietilenoglicóis/química , Ácidos Polimetacrílicos/química , Dióxido de Silício/química
9.
Shokuhin Eiseigaku Zasshi ; 58(1): 32-35, 2017.
Artigo em Japonês | MEDLINE | ID: mdl-28260730

RESUMO

Kuwazuimo (Alocasia odora) and shimakuwazuimo (Alocasia cucullata) are evergreen perennial plants that originated in East Asia. Although inedible, they are occasionally eaten by mistake because they resemble satoimo (Colocasia esculenta), and this has caused food poisoning in Japan. It is not easy to determine the cause of a food poisoning outbreak from the shape or chemical composition when the available sample is small. Therefore, we developed a new primer pair for PCR to identify kuwazuimo and shimakuwazuimo in small samples, based on the internal transcribed spacer (ITS) region of ribosomal DNA. Using PCR with the developed primer pair, we detected all samples of kuwazuimo obtained from the market, while excluding 17 other kinds of crops. The samples were identified as shimakuwazuimo by DNA sequencing of the PCR products. The present PCR method showed high specificity and was confirmed to be applicable to the identification of kuwazuimo and shimakuwazuimo from various crops.


Assuntos
Alocasia/química , Alocasia/genética , Análise de Alimentos/métodos , Contaminação de Alimentos/análise , Reação em Cadeia da Polimerase/métodos , Alocasia/envenenamento , DNA Ribossômico , Doenças Transmitidas por Alimentos/etiologia , Doenças Transmitidas por Alimentos/prevenção & controle , Sensibilidade e Especificidade , Análise de Sequência de DNA
10.
Medicine (Baltimore) ; 96(6): e6056, 2017 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-28178155

RESUMO

Secondary thrombotic microangiopathies (TMAs) are induced by several underlying conditions; most are resolved by treating background disease. Eculizumab is a human monoclonal antibody that blocks the final stage of the complement system and effectively treats atypical hemolytic uremic syndrome (aHUS). In this report, we present a patient with TMA secondary to sepsis- induced coagulopathy, who was successfully treated with eculizumab.A 44-year-old woman, who had no special medical history or familial history of TMAs, was admitted on suspicion of septic shock. Physical examination revealed gangrene on her soles. Blood tests revealed a decreased platelet count, disseminated intravascular coagulation (DIC), renal dysfunction, hemolysis, and infection. Although the coagulation disorder improved with intensive care, the low platelet count, elevated lactate dehydrogenase levels, and renal dysfunction persisted. Our investigations subsequently excluded thrombotic thrombocytopenic purpura and Shiga toxin-producing Escherichia coli-induced HUS. Plasma exchange only improved lactate dehydrogenase levels. We clinically diagnosed this case as atypical HUS and started eculizumab treatment. The patient's platelet count increased, her renal dysfunction improved, and the gangrene on her feet was ameliorated. The patient was discharged without maintenance dialysis therapy after approximately 3 months. Subsequent tests revealed elevated serum levels of soluble C5b-9, and genetic testing revealed compound heterozygous c.184G > A (Val62Ile) and c.1204T > C (Tyr402His) single-nucleotide polymorphisms in complement factor H.We encountered a case of complement-mediated TMA accompanied by DIC, which was successfully treated with eculizumab. Further studies are necessary to support the optimal use of eculizumab for TMA with background diseases.


Assuntos
Anticorpos Monoclonais Humanizados/uso terapêutico , Inativadores do Complemento/uso terapêutico , Coagulação Intravascular Disseminada/complicações , Microangiopatias Trombóticas/tratamento farmacológico , Microangiopatias Trombóticas/etiologia , Adulto , Coagulação Intravascular Disseminada/etiologia , Feminino , Humanos , Testes de Função Renal , Contagem de Plaquetas , Sepse/complicações , Microangiopatias Trombóticas/imunologia
11.
Endocrinology ; 157(10): 3994-4002, 2016 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-27560548

RESUMO

Reproduction is essential for life, but its regulatory mechanism is diverse. The analysis of this diversity should lead us to understand the evolutionary process of the regulation of reproduction. In mammals, the hypothalamic-pituitary-gonadal axis plays an essential role in such regulation, and each component, hypothalamic GnRH, and pituitary gonadotropins, LH, and FSH, is indispensable. However, the common principle of the hypothalamic-pituitary-gonadal axis regulation among vertebrates remains unclear. Here, we used a teleost medaka, which is phylogenetically distant from mammals, and analyzed phenotypes of gene knockouts (KOs) for GnRH, LH, and FSH. We showed that LH release, which we previously showed to be directly triggered by GnRH, is essential for ovulation in females, because KO medaka of GnRH and LH were anovulatory in spite of the full follicular growth and normal gonadosomatic index, and spawning could be induced by a medaka LH receptor agonist. On the other hand, we showed that FSH is necessary for the folliculogenesis, because the follicular growth of FSH KO medaka was halted at the previtellogenic stage, but FSH release does not necessarily require GnRH. By comparing these results with the previous studies in mammals that both GnRH and LH are necessary for folliculogenesis, we propose a hypothesis as follows. During evolution, LH was originally specialized for ovulation, and regulation of folliculogenesis by GnRH-LH (pulsatile release) was newly acquired in mammals, which enabled fine tuning of reproduction through hypothalamus.


Assuntos
Evolução Biológica , Hormônio Foliculoestimulante/fisiologia , Hormônio Liberador de Gonadotropina/fisiologia , Hormônio Luteinizante/fisiologia , Reprodução , Animais , Feminino , Fertilidade , Técnicas de Inativação de Genes , Gonadotropinas Equinas , Gônadas/fisiologia , Sistema Hipotálamo-Hipofisário , Masculino , Oryzias
12.
J Rural Med ; 11(1): 25-9, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27313799

RESUMO

OBJECTIVE: Rescuing severe trauma patients who are injured far from a trauma center is challenging for rural emergency systems. We report a severe trauma case that occurred at a remote location, in which the patient's life was saved by a dispatched doctor car and a physician from a local medical facility. PATIENT: A 31-year-old man experienced a left femur injury due to a fallen tree. The fire station requested a doctor car from our center, approximately 56 km away. Meanwhile, a paramedic team reported that the patient was in a state of shock. The doctor car docked over 1 h after the accident. Pressure hemostasis, rapid intravenous infusion, and tracheal intubation were performed en route. After arrival at our hospital, an emergency blood transfusion was administered; the injured blood vessel was sutured and the wound closed. On day 22, the patient was transferred to another hospital for rehabilitation. DISCUSSION: Rapid response-type doctor car is often considered ineffective for distant severe trauma cases. However, this case demonstrates the benefits of a doctor car working with local medical facilities. CONCLUSION: The rapid response-type doctor car is effective even in remote severe trauma cases.

13.
J Nat Med ; 68(4): 677-85, 2014 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-24952707

RESUMO

In May 2011, numerous poppy plants closely resembling Papaver bracteatum Lindl., a type of narcotic plant that is illegal in Japan, were distributed directly from several large flower shops or through online shopping throughout Japan, including the Tokyo Metropolitan area. In order to better identify the narcotic plants, the relative nuclear DNA content at the vegetative stage was measured by flow cytometric (FCM) analysis in 3 closely-related species of the genus Papaver section Oxytona, namely P. orientale, P. pseudo-orientale, and P. bracteatum, based on the difference between the chromosome numbers of these species. The results showed that the nuclear DNA content differed between these 3 species, and that most of the commercially distributed plants examined in this study could be identified as P. bracteatum. The remaining plants were P. pseudo-orientale, a non-narcotic plant. In addition, the FCM results for the identification of P. bracteatum completely agreed with the results obtained by the morphological analysis, the inter-genic spacer sequence of rpl16-rpl14 (PS-ID sequence) of chloroplast DNA, and the presence of thebaine. These results clearly indicate the usefulness of FCM analysis for the identification of P. bracteatum plants, including when they are in their vegetative stage.


Assuntos
Citometria de Fluxo , Papaver/classificação , DNA de Cloroplastos/química , Flores/química , Japão , Entorpecentes/análise , Papaver/anatomia & histologia , Papaver/química , Papaver/genética , Tebaína/análise
14.
Cell Signal ; 25(3): 589-97, 2013 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-23200849

RESUMO

Gα(h) (or transglutaminase-2 (TG2)) is an atypical guanine nucleotide binding-protein that associates with G protein-coupled receptors. TG2 also exerts transglutaminase activity that catalyzes posttranslational protein cross-linking with the formation of ε-(γ-glutamyl) lysine or (γ-glutamyl) polyamine bonds. Here, the role of Gα(h)/TG2 in signal transduction in glial cells was examined in detail. In 1321N1 human astrocytoma cells that lack Gα(h)/TG2, overexpression of Gα(h)/TG2 caused an enhancement of cAMP accumulation stimulated with the ß-adrenergic receptor agonist, isoproterenol, or the adenylylcyclase activator, forskolin. This cAMP-enhancement was reversed by the TG2 inhibitor, ERW1069. In rat C6 glioma cells that express endogenous Gα(h)/TG2, cAMP accumulation induced by isoproterenol or forskolin was significantly inhibited by overexpression of Gα(h)/TG2-C277V, a dominant-negative mutant that lacks transglutaminase activity, but was not inhibited by the Gα(h)/TG2-S171E mutant that cannot bind GTP/GDP. These results suggest Gα(h)/TG2 potentiates adenylylcyclase activity by its transglutaminase activity and not by its G-protein activity. Gα(h)/TG2 also increased the activities of the cAMP response element and interleukin-6 promoter, accompanied by an of cAMP in both glioma cells. Since adenylylcyclase 8 plays a major role in cAMP production, we focused on post-translational modification of adenylylcyclase 8 by Gα(h)/TG2. Adenylylcyclase 8 is expressed in both 1321N1 and C6 cells; however, Gα(h)/TG2 affected neither adenylylcyclase 8 expression levels, glycosylation, nor dimerization status. In contrast, pentylamine, a substrate of Gα(h)/TG2, was incorporated into adenylylcyclase 8 in a transglutaminase activity-dependent manner. Taking these results together, Gα(h)/TG2 promotes cAMP production accompanied by a modification of adenylylcyclase 8 in glioma cells.


Assuntos
Adenilil Ciclases/metabolismo , Proteínas de Ligação ao GTP/metabolismo , Transglutaminases/metabolismo , Substituição de Aminoácidos , Animais , Linhagem Celular Tumoral , Colforsina/farmacologia , AMP Cíclico/metabolismo , Ativação Enzimática/efeitos dos fármacos , Proteínas de Ligação ao GTP/antagonistas & inibidores , Proteínas de Ligação ao GTP/genética , Glioma/metabolismo , Glioma/patologia , Humanos , Interleucina-6/genética , Interleucina-6/metabolismo , Isoproterenol/farmacologia , Regiões Promotoras Genéticas , Ratos , Transdução de Sinais/efeitos dos fármacos , Transglutaminases/antagonistas & inibidores , Transglutaminases/genética
15.
J Pharm Pharmacol ; 64(5): 670-6, 2012 May.
Artigo em Inglês | MEDLINE | ID: mdl-22471362

RESUMO

OBJECTIVES: Many patients use herbal medicines to relieve menopausal symptoms. Keishi-bukuryo-gan contains five herbal components, and has been used for treating hypermenorrhoea, dysmenorrhoea and menopausal symptoms in Asian countries. In this study, we investigated the potential herb-drug interactions of keishi-bukuryo-gan in healthy female subjects. METHODS: Thirty-one healthy females (20-27 years) were studied to evaluate their baseline activity of cytochrome P450 (CYP) 1A2, CYP2D6, CYP3A, xanthine oxidase (XO) and N-acetyltransferase 2 (NAT2) based on the urinary metabolic indices of an 8-h urine sample collected after a 150-mg dose of caffeine and a 30-mg dose of dextromethorphan, and also the urinary excretion ratio of 6ß-hydroxycortisol to cortisol. Thereafter, the subjects received 3.75g of keishi-bukuryo-gan twice daily for seven days, and underwent the same tests on post-dose day 7. KEY FINDINGS: The geometric mean phenotypic index for CYP1A2 significantly decreased by 16% on day 7 compared with the baseline (P=0.026). Keishi-bukuryo-gan did not alter the indices for CYP2D6, CYP3A, XO and NAT2. CONCLUSIONS: Keishi-bukuryo-gan may inhibit the activity of CYP1A2, which is predominantly involved in oestrogen metabolism. However, TJ-25 is unlikely to participate in herb-drug interactions involving medications predominantly metabolized by CYP2D6, CYP3A, XO and NAT2. K


Assuntos
Cafeína/metabolismo , Inibidores do Citocromo P-450 CYP1A2 , Dextrometorfano/metabolismo , Medicamentos de Ervas Chinesas/farmacologia , Interações Ervas-Drogas , Hidrocortisona/metabolismo , Acetiltransferases/metabolismo , Adulto , Cafeína/urina , Citocromo P-450 CYP2D6/metabolismo , Citocromo P-450 CYP3A/metabolismo , Dextrometorfano/urina , Medicamentos de Ervas Chinesas/uso terapêutico , Estrogênios/metabolismo , Feminino , Humanos , Hidrocortisona/urina , Menopausa , Fenótipo , Fitoterapia/efeitos adversos , Valores de Referência , Xantina Oxidase/metabolismo , Adulto Jovem
16.
Bioconjug Chem ; 22(5): 887-93, 2011 May 18.
Artigo em Inglês | MEDLINE | ID: mdl-21488636

RESUMO

Gold magnetic particles (GMP) are magnetic iron oxide particles modified with gold nanoparticles. The gold particles of GMP specifically bind to cysteine and methionine through Au-S binding. The aim of the present study was to establish a quick and easy protein purification system using novel peptide tags and GMP. Here, we created a variety of peptide tags containing methionine and cysteine and analyzed their affinity to GMP. Binding assays using enhanced green fluorescent protein (EGFP) as a model protein indicated that the tandem methionine tags comprising methionine residues had higher affinity to the GMP than tags comprising both methionine and cysteine residues. Tags comprising both methionine and glycine residues showed slightly higher affinity to GMP and higher elution efficiency than the all-methionine tags. A protein purification assay using phosphorylcholine-treated GMP demonstrated that both a tandem methionine-tagged EGFP and a methionine and glycine-tagged EGFP were specifically purified from a protein mixture with very high efficiency. The efficiency was comparable to that of a histidine-tagged protein purification system. Together, these novel peptide tags, "methionine tags", specifically bind to GMP and can be used for a highly efficient protein purification system.


Assuntos
Ouro/química , Proteínas de Fluorescência Verde/isolamento & purificação , Magnetismo , Nanopartículas Metálicas/química , Metionina/química , Peptídeos/química , Eletroforese em Gel de Poliacrilamida , Compostos Férricos/química , Proteínas de Fluorescência Verde/química , Tamanho da Partícula , Fosforilcolina/química , Ligação Proteica , Propriedades de Superfície
17.
Ophthalmic Res ; 36(6): 321-6, 2004.
Artigo em Inglês | MEDLINE | ID: mdl-15627832

RESUMO

PURPOSE: Two isoforms of cyclooxygenase (COX-1 and COX-2) exist. To determine in vivo effects of the intravenous administration of FR122047 (a selective COX-1 inhibitor), FR188582 (a selective COX-2 inhibitor), diclofenac sodium or dexamethasone phosphate disodium on prostaglandin-E2 (PGE2)-induced aqueous flare elevation and mRNA levels for COX-1 and COX-2 in pigmented rabbits. METHODS: To produce aqueous flare elevation in rabbits, PGE2, 25 microg/ml, was applied to the cornea with the use of a glass cylinder. FR122047, FR188582, diclofenac sodium or dexamethasone phosphate disodium was intravenously injected before PGE2 application. Aqueous flare was measured with a laser flare-cell meter. The mRNA levels for COX-1 and COX-2 in the iris-ciliary body were determined by real-time polymerase chain reaction. RESULTS: FR122047, FR188582 and diclofenac sodium (15 micromol/kg each) injected intravenously 30 min before PGE2 application inhibited 29 +/- 5, 40 +/- 12 and 50 +/- 9% of aqueous flare elevation, respectively. Simultaneous injection of FR122047 (15 micromol/kg) and FR188582 (15 micromol/kg) 30 min before PGE2 application inhibited 61 +/- 8% of flare elevation. Dexamethasone phosphate disodium (15 micromol/kg) injected intravenously 300 min before PGE2 application inhibited 68 +/- 8% of aqueous flare elevation. Less than 3-fold changes in mRNA levels for COX-1 and COX-2 in the iris-ciliary body were noted after PGE2, FR122047, FR188582, diclofenac sodium or dexamethasone phosphate disodium treatment. CONCLUSION: It is possible that enzyme activities of both COX-1 and COX-2 may be involved in the mechanism of PGE2-induced aqueous flare elevation in pigmented rabbits.


Assuntos
Humor Aquoso/efeitos dos fármacos , Inibidores de Ciclo-Oxigenase/farmacologia , Dinoprostona/toxicidade , Isoenzimas/antagonistas & inibidores , Piperazinas/farmacologia , Pirazóis/farmacologia , Tiazóis/farmacologia , Uveíte Anterior/prevenção & controle , Animais , Anti-Inflamatórios não Esteroides/farmacologia , Humor Aquoso/metabolismo , Barreira Hematoaquosa/efeitos dos fármacos , Corpo Ciliar/enzimologia , Ciclo-Oxigenase 1 , Ciclo-Oxigenase 2 , Inibidores de Ciclo-Oxigenase 2 , Relação Dose-Resposta a Droga , Quimioterapia Combinada , Injeções Intravenosas , Iris/enzimologia , Isoenzimas/genética , Masculino , Prostaglandina-Endoperóxido Sintases/genética , RNA Mensageiro/metabolismo , Coelhos , Uveíte Anterior/induzido quimicamente , Uveíte Anterior/enzimologia
18.
Am J Chin Med ; 31(5): 729-38, 2003.
Artigo em Inglês | MEDLINE | ID: mdl-14696676

RESUMO

The purpose of this study was to evaluate the effects of extracts of Coptidis rhizoma, Phellodendri cortex and Gardeniae fructus, which are medicinal herbs in Orengedoku-to (Huanglin-Jie-Du-Tang in Chinese), and crocetin (a major component of Gardeniae fructus) on experimental elevation of aqueous flare in pigmented rabbits. To produce aqueous flare elevation, 0.5 microg/kg lipopolysaccharide (LPS) was injected into the ear vein, or prostaglandin E2 (PGE2) 25 microg/ml, was applied to the cornea by means of a glass cylinder. Animals were pretreated by oral administration of 150 g/day of food containing 0.15% (w/w) extract powder of Coptidis rhizoma, 0.10% (w/w) extract powder of Phellodendri cortex or 0.15% (w/w) extract powder of Gardeniae fructus for 4 days, or by intravenous injection of crocetin, 0.3, 3, 30 or 300 microg/kg, 30 minutes before aqueous flare elevation. Aqueous flare was measured with a laser flare-cell meter. Aqueous flare intensity was expressed as the area under the curve (AUC) in arbitrary units. The AUC of LPS- and PGE2-induced aqueous flare elevation was 4685 and 1386 arbitrary units, respectively. Pretreatment by oral administration of 0.15% (w/w) extract of Coptidis rhizoma or 0.10% (w/w) extract of Phellodendri cortex did not inhibit LPS-induced aqueous flare elevation. Pretreatment by oral administration of 0.15% extract of Gardeniae fructus suppressed LPS-induced aqueous flare elevation (AUC: 1411 arbitrary units). Pretreatment by intravenous injection of 3, 30 or 300 microg/kg of crocetin-inhibited LPS-induced aqueous flare elevation in a dose-dependent manner. Pretreatment with 3 or 30 microg/kg of crocetin did not inhibit PGE2-induced aqueous flare elevation, but 300 microg/kg of crocetin inhibited PGE2-induced aqueous flare elevation (AUC: 918 arbitrary units).


Assuntos
Anti-Inflamatórios não Esteroides/farmacologia , Antioxidantes/farmacologia , Humor Aquoso/efeitos dos fármacos , Carotenoides/farmacologia , Medicamentos de Ervas Chinesas/farmacologia , Administração Oral , Animais , Área Sob a Curva , Dinoprostona , Modelos Animais de Doenças , Relação Dose-Resposta a Droga , Injeções Intravenosas , Lipopolissacarídeos , Masculino , Coelhos , Fatores de Tempo , Uveíte Anterior/induzido quimicamente , Uveíte Anterior/tratamento farmacológico
19.
J Cataract Refract Surg ; 29(1): 20-6, 2003 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-12551662

RESUMO

PURPOSE: To determine whether endophthalmitis after small-incision cataract surgery is affected by the incision site (superior sclerocorneal versus temporal cornea) or the foldable intraocular lens (IOL) material (silicone versus acrylic). SETTING: Multicenter study. METHODS: Patients who had small-incision cataract surgery at Toyama Medical and Pharmaceutical University Hospital and affiliated hospitals from March 1998 to March 2001 were examined prospectively. The patients were randomized into 3 groups. In Group A, an acrylic IOL (MA60BM, Alcon) was implanted through a temporal corneal incision. In Group B, an acrylic IOL (MA60BM) was implanted via a superior sclerocorneal incision. In Group C, a silicone poly(methyl methacrylate) IOL (SI-40NB, Allergan) was implanted via a superior sclerocorneal incision. Each patient was followed for more than 6 months. RESULTS: Initially, 7622 patients (12 317 eyes) agreed to participate in the study. The final study included 3831 eyes in Group A, 3901 eyes in Group B, and 3863 eyes in Group C. Postoperative endophthalmitis was diagnosed clinically in 11 eyes (0.29%) in Group A, 2 (0.05%) in Group B, and 2 (0.05%) in Group C. Endophthalmitis proven by culture was found in 9 eyes in Group A, 2 in Group B, and 2 in Group C. The incidence of endophthalmitis in Group A was higher than in the other groups. The incidence of endophthalmitis in Group B was similar to that in Group C. The relative risk of postoperative endophthalmitis proven by culture in Groups B and C was 4.6 times (P =.037) lower than in Group A. CONCLUSION: The findings suggest that a temporal corneal incision may lead to an increased risk of postoperative endophthalmitis and that the IOL material does not affect the incidence of this complication.


Assuntos
Infecções Bacterianas/etiologia , Extração de Catarata/efeitos adversos , Endoftalmite/microbiologia , Lentes Intraoculares/efeitos adversos , Acrilatos , Idoso , Idoso de 80 Anos ou mais , Extração de Catarata/métodos , Córnea/cirurgia , Endoftalmite/epidemiologia , Desenho de Equipamento , Feminino , Humanos , Incidência , Masculino , Polimetil Metacrilato , Estudos Prospectivos , Esclera/cirurgia
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