Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 72
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Biomed Res ; 36(2): 135-42, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-25876664

RESUMO

The effect of repetitive mild hyperthermia on body temperature, the autonomic nervous system, and innate and adaptive immunity was investigated using a new hyperthermia treatment system, nanomist sauna (NMS). Six healthy volunteers participated and the concentration of catecholamines and cortisol, and the frequency and function of leukocytes in the peripheral blood were investigated before and after successive 7 days of hyperthermia treatment (20 min/day, 40°C, 100% relative humidity). After treatment, the blood level of adrenaline and cortisol on the 7th day was decreased compared with the 1st day, indicating the suppression of the sympathetic nervous system activity. Moreover, the frequency of CD56(+)NK, CD56(+)NKT and B cells on the 7th day tended to be increased compared with the 1st day. The frequency of HLA-DR-positive NK and NKT cells and expression of HLA-DR on B and T cells increased. The cytotoxicity of NK cells and proliferative response of B cells were also elevated. The results indicate that repetitive mild hyperthermia treatment might suppress excessive sympathetic dominance and modify immunity. Additionally, because it can provide the same effects as conventional hyperthermia treatments with minimal burden to the body, NMS may be a novel patient- and elderly-friendly hyperthermia treatment for health promotion.


Assuntos
Imunidade Adaptativa , Sistema Nervoso Autônomo/metabolismo , Hipertermia Induzida , Imunidade Inata , Adulto , Linfócitos B/metabolismo , Temperatura Corporal , Epinefrina/sangue , Antígenos de Histocompatibilidade Classe II/metabolismo , Humanos , Hidrocortisona/sangue , Masculino , Pessoa de Meia-Idade , Células T Matadoras Naturais/metabolismo , Norepinefrina/sangue
2.
Cell Immunol ; 289(1-2): 162-6, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-24838093

RESUMO

In order to further examine the reactivity of autoantibodies, mice were infected with a non-lethal strain of Plasmodium yoelii. Parasitemia appeared between days 10 and 21. During this period, hyperglycemia and hypothermia were serially obeserved and this phenomenon resembled stress-associated responses. In parallel with parasitemia, autoantibodies appeared against nucleus and double-stranded DNA in the sera. To examine further the reactivity of autoantibodies against tissues, immunohistochemical staining using sera from mice with or without malaria was conducted. Autoantibodies contained reactivity to erythrocytes in the spleen, bone marrow and peripheral blood, especially against tissues obtained from mice with malaria. In the liver and intestine, autoantibodies reacted with hepatocytes and intestinal epithelial cells, respectively. These results suggested that the reactivity of autoantibodies against erythrocytes and hepatocytes might be associated with the modulation of the disease course in malaria.


Assuntos
Autoanticorpos/imunologia , Eritrócitos/imunologia , Hepatócitos/imunologia , Malária/imunologia , Plasmodium yoelii/imunologia , Animais , Anticorpos Antinucleares/sangue , Anticorpos Antinucleares/imunologia , Antígenos de Protozoários/imunologia , Autoanticorpos/sangue , Hiperglicemia , Hipotermia , Mucosa Intestinal/citologia , Mucosa Intestinal/imunologia , Fígado/citologia , Fígado/imunologia , Camundongos , Camundongos Endogâmicos C57BL , Parasitemia/sangue , Parasitemia/imunologia , Baço/imunologia
3.
Med Hypotheses ; 83(1): 99-100, 2014 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-24767936

RESUMO

Capillary vessel flow in the base of the fingernail can be observed by microscopy. This flow is switched off under some conditions, such as coldness, surprise, and anger and is switched on again under other conditions, such as warming, relaxation, and mild exercise. In other words, capillary vessels perform two functions: switching flow on and off. It is speculated that the switch-off function is necessary to direct energy production to the glycolysis pathway, while the switch-on function is necessary for the mitochondrial pathway. This is because glycolysis takes place under anaerobic conditions, while oxidative phosphorylation in the mitochondria proceeds under aerobic conditions in the body. To switch off circulation, the negative electric charges on the surface of erythrocytes and the capillary wall may be decreased by stimulation of the sympathetic nerves and secretion of steroid hormones. Negative charge usually acts as repulsive force between erythrocytes and between erythrocytes and the capillary wall. By decreasing the negative charge, erythrocytes can aggregate and also adhere to the capillary wall. These behaviors may be related to the capillary flow switch-off function. Here, it is emphasized that the capillary vessels possess not only a switch-on function but also a switch-off function for circulation.


Assuntos
Capilares/fisiologia , Metabolismo Energético , Glicólise , Mitocôndrias/metabolismo , Humanos
4.
World J Gastroenterol ; 19(37): 6258-64, 2013 Oct 07.
Artigo em Inglês | MEDLINE | ID: mdl-24115824

RESUMO

AIM: To investigate the stress-induced apoptosis of natural killer (NK) cells and the changes in their killing activity in mouse livers. METHODS: A restraint stress model was established in mice. Flow cytometry was employed to measure the percentage of NK cells and the changes in their absolute number in mouse liver. The cytotoxicity of hepatic and splenic NK cells was assessed against YAC-1 target cells via a 4 h 51Cr-release assay. RESULTS: The restraint stress stimulation induced the apoptosis of NK cells in the liver and the spleen, which decreased the cell number. The number and percentage of NK cells in the spleen decreased. However, the number of NK cells in the liver decreased, whereas the percentage of NK cells was significantly increased. The apoptosis of NK cells increased gradually with prolonged stress time, and the macrophage-1 (Mac-1)(+) NK cells were more susceptible to apoptosis than Mac-1(-) NK cells. Large numbers of Mac-1(-) NK cells in the liver, which are more resistant to stress-induced apoptosis, were observed than the Mac-1(-) NK cells in the spleen. The stress stimulation diminished the killing activity of NK cells in the spleen was significantly decreased, but the retention of numerous Mac-1(-) NK cells in the liver maintained the killing ability. CONCLUSION: Significant stress-induced apoptosis was observed among Mac-1(+) NK cells, but not Mac-1(-) NK cells in the mouse liver. Stress stimulation markedly decreased the killing activity of NK cells in the spleen but remained unchanged in the liver.


Assuntos
Apoptose , Células Matadoras Naturais/patologia , Fígado/patologia , Restrição Física/psicologia , Baço/patologia , Estresse Psicológico/patologia , Animais , Biomarcadores/análise , Células Matadoras Naturais/imunologia , Fígado/imunologia , Antígeno de Macrófago 1/análise , Camundongos , Camundongos Endogâmicos C57BL , Baço/imunologia , Estresse Psicológico/imunologia
5.
Med Hypotheses ; 80(6): 813-5, 2013 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-23557848

RESUMO

As eukaryotes, human beings spend their lifetime using two different pathways for energy production: the anaerobic glycolysis pathway and aerobic mitochondria pathway. Energy produced using the glycolysis pathway is utilized for cell division and prompt force of white fiber muscles whereas that produced by the mitochondria pathway is utilized for suppression of cell division and continuous force of red fiber muscles. Herein, it is proposed that there exists a sequential shift of energy production pathways at the fetal stage and during the lifetime. If we introduce this concept in human biology, then it is may be easy to understand the energy requirement characteristics of fetuses, children, adults and aged persons. For example, during childhood the glycolysis pathway is more predominant than the mitochondria pathway in energy production. Reflecting this fact, children grow and extend their height by division of generalized cells of the body. To achieve this, they have to eat meals many times a day (more than three times a day) due to a less efficient energy production rate in the glycolysis pathway. Similarly, the characteristics of adults and aged persons can be well described using this concept.


Assuntos
Metabolismo Energético/fisiologia , Desenvolvimento Fetal/fisiologia , Glicólise/fisiologia , Desenvolvimento Humano/fisiologia , Modelos Biológicos , Fosforilação Oxidativa , Fatores Etários , Divisão Celular/fisiologia , Humanos
6.
Immunobiology ; 218(1): 10-20, 2013 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-22361243

RESUMO

Although it has been hypothesized that autoimmune-like phenomena may play a critical role in the protective immune responses to both human and animal malaria, there are still no evidence-based data to support this view. In this study we demonstrate that the majority of anti-single stranded (ss) DNA autoantibody secreting B cells were confined to B220(+)CD21(+)CD23(-) cells and that these cells expanded significantly in the spleen of C57BL/6 mice infected with Plasmodium yoelii 17X non-lethal (PyNL). To determine the role of ssDNA-binding autoantibody secreting B cell responses in murine malaria, we conjugated generation 6 (poly) amidoamine dendrimer nanoparticles with ssDNA to deplete ssDNA-binding autoreactive B cells in vivo. Our data revealed that 55.5% of mice died after DNA-coated nanoparticle-mediated in vivo depletion of ssDNA-specific autoreactive B cells and subsequent challenge using PyNL. Adoptive transfer of B cells with ssDNA specificity to mice, followed by PyNL infection, caused a later appearance and inhibition of parasitemia. The possible mechanism by which the ssDNA-binding autoantibody secreting B cells is involved in the protection against murine malaria has also been demonstrated.


Assuntos
Anticorpos Antinucleares/imunologia , Linfócitos B/imunologia , DNA de Cadeia Simples/imunologia , Malária/imunologia , Plasmodium yoelii/imunologia , Baço/imunologia , Transferência Adotiva , Animais , Linfócitos B/parasitologia , Células Cultivadas , Modelos Animais de Doenças , Humanos , Imunidade Ativa/efeitos dos fármacos , Imunidade Humoral/efeitos dos fármacos , Depleção Linfocítica , Malária/terapia , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Nanopartículas/administração & dosagem
7.
Biomed Res ; 33(4): 211-6, 2012.
Artigo em Inglês | MEDLINE | ID: mdl-22975631

RESUMO

We studied the effect of acute stress on mouse immune system and found that the stress modified the proteins existing in the lymphocytes of liver. Performing SDS-polyacrylamide gel electrophoresis (SDS-PAGE), we got the result that the proteins existing in the lymphocytes were different between stressed liver and non-stressed liver, whereas we could not find any detectable differences in the lymphocyte proteins between stressed spleen and non-stressed spleen. In stressed liver lymphocytes, we found thirteen clear bands in the stained gel that were not prominent in non-stressed liver lymphocytes.


Assuntos
Fígado/metabolismo , Linfócitos/metabolismo , Estresse Psicológico/imunologia , Animais , Complexo CD3/metabolismo , Eletroforese em Gel de Poliacrilamida , Citometria de Fluxo , Imunofluorescência/métodos , Fígado/imunologia , Linfócitos/imunologia , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Proteoma/análise , Baço/imunologia , Baço/metabolismo , Fatores de Tempo
8.
Biomed Res ; 33(4): 243-8, 2012.
Artigo em Inglês | MEDLINE | ID: mdl-22975635

RESUMO

Skin rubdown using a dry towel (SRDT) to scrub the whole body is a traditional therapy for health promotion. To investigate its mechanism, 24 healthy male volunteers were studied. Body temperature, pulse rate, red blood cells (RBCs), serum levels of catecholamines and cortisol, blood gases (PO(2), sO(2), PCO(2) and pH), lactate and glucose, and the ratio and number of white blood cells (WBCs) were assessed before and after SRDT. After SRDT, pulse rate and body temperature were increased. PO(2), sO(2) and pH were also increased and there was no Rouleaux formation by RBCs. Lactate level tended to increase, whereas that of glucose did not. Adrenaline and noradrenaline levels increased, indicating sympathetic nerve (SN) dominance with increase in granulocytes. WBC number and ratio were divided into two groups according to granulocyte ratio (≤ or < 60%) before SRDT: a normal group and a SN group. Only in the SN group did the granulocyte ratio decrease and the lymphocyte ratio and number increase after SRDT. It is suggested that SRDT is a mild aerobic, systemic exercise that might affect the immune system via the autonomic nervous system.


Assuntos
Sistema Nervoso Autônomo/fisiologia , Temperatura Corporal/fisiologia , Metabolismo Energético , Terapia por Exercício/métodos , Exercício Físico/fisiologia , Sistema Imunitário/fisiologia , Adulto , Gasometria , Glicemia , Catecolaminas/sangue , Agregação Eritrocítica , Contagem de Eritrócitos/métodos , Granulócitos/fisiologia , Frequência Cardíaca/fisiologia , Humanos , Hidrocortisona/sangue , Concentração de Íons de Hidrogênio , Ácido Láctico/análise , Masculino , Massagem , Pessoa de Meia-Idade , Neutrófilos/fisiologia , Pele/metabolismo , Fenômenos Fisiológicos da Pele , Adulto Jovem
9.
Immunology ; 136(4): 448-58, 2012 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-22564028

RESUMO

Macrophages are the major source of the chemokines macrophage inflammatory protein-2 (MIP-2) and keratinocyte-derived chemokine (KC), which play a major role in neutrophil migration to sites of inflammation. Although extracellular ATP from inflammatory tissues induces several immune responses in macrophages, it is unclear whether ATP-stimulated macrophages affect neutrophil migration. Therefore, the aim of the present study was to investigate the role of ATP-induced MIP-2 production by macrophages. When ATP was injected intraperitoneally into mice, the number of neutrophils within the peritoneal cavity markedly increased, along with the levels of MIP-2 and KC in the peritoneal lavage fluid. Consistent with this, ATP induced MIP-2 production, but not that of KC, by peritoneal exudate macrophages (PEMs) in vitro. This occurred via interactions with the P2X(7) receptor and P2Y(2) receptor. Furthermore, treatment of PEMs with ATP led to the production of reactive oxygen species. The ATP-induced MIP-2 production was inhibited by treatment with the antioxidant N-acetyl-l-cysteine. Also, MIP-2 production was inhibited by pre-incubating PEMs with inhibitors of extracellular signal-regulated kinase 1/2 or p38 mitogen-activated protein kinase. The MIP-2 neutralization reduced the increase in neutrophil numbers observed in ATP-treated mice. Taken together, these results suggest that increased production of reactive oxygen species by ATP-stimulated macrophages activates the signalling pathways that promote MIP-2 production which, in turn, induces neutrophil migration.


Assuntos
Trifosfato de Adenosina/farmacologia , Quimiocina CXCL2/metabolismo , Quimiotaxia de Leucócito , Macrófagos Peritoneais/metabolismo , Neutrófilos/imunologia , Acetilcisteína/farmacologia , Animais , Movimento Celular , Células Cultivadas , Quimiocinas/metabolismo , MAP Quinases Reguladas por Sinal Extracelular/antagonistas & inibidores , Macrófagos Peritoneais/imunologia , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Transgênicos , Neutrófilos/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Receptores Purinérgicos P2X7/metabolismo , Receptores Purinérgicos P2Y2/metabolismo , Proteínas Quinases p38 Ativadas por Mitógeno/antagonistas & inibidores
10.
Immunol Res ; 52(3): 224-30, 2012 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-22477526

RESUMO

Cumulative evidence has shown that extrathymic T cells can be autoreactive and that B-1 cells may produce autoantibodies. These T and B-1 cells, which form part of the innate immune system, tend to be activated simultaneously when conventional T and B cells are in a suppressive state, for example, when thymic atrophy occurs by stress or involution with aging. In other words, autoreactive T cells and autoantibody-producing B cells are different from thymus-derived T cells and bone marrow-derived B cells. Activated extrathymic T cells and B-1 cells are often observed in numerous autoimmune diseases, aging, malarial infection and chronic graft-versus-host disease. It is thought that the autoreactivity of extrathymic T cells and B-1 cells may be important for the elimination of "abnormal self" tissues or cells. However, over-activation of innate lymphocytes may be related to the onset of disease or self-tissue destruction. However, it must be emphasized that the autoreactivity of innate lymphocytes is not generated by failure of the thymic pathway of T-cell differentiation or the conventional pathway of B-2 cells.


Assuntos
Autoimunidade , Subpopulações de Linfócitos B/imunologia , Células da Medula Óssea/imunologia , Imunidade Inata , Linfócitos T/imunologia , Envelhecimento/imunologia , Animais , Doenças Autoimunes/imunologia , Doença Crônica , Doença Enxerto-Hospedeiro/imunologia , Humanos , Malária/imunologia , Camundongos , Timo/imunologia
11.
Immunology ; 136(1): 86-95, 2012 May.
Artigo em Inglês | MEDLINE | ID: mdl-22268994

RESUMO

Wild-type (WT) and CD1d(-/-) [without natural killer (NK) T cells] mice were treated with zymosan A to induce granuloma formation in the liver. Increased granuloma formation was seen in NKT-less mice on days 7 and 14 after administration. WT mice showed limited granuloma formation, and zymosan A eventually induced NKT cell accumulation as identified by their surface marker (e.g. CD1d-tetramer). Zymosan A augmented the expression of Toll-like receptor 2 on the cell surface of both macrophages and NKT cells. One possible reason for accelerated granuloma formation in NKT-less mice was increased production of interferon- γ (IFN-γ); a theory that was confirmed using IFN-γ(-/-) mice. Also, zymosan A increased interleukin-10 production in WT mice, which suppresses IFN-γ production. Taken together, these results suggest that NKT cells in the liver have the potential to suppress zymosan A-mediated granuloma formation.


Assuntos
Vesículas Citoplasmáticas/imunologia , Interferon gama/imunologia , Interleucina-10/imunologia , Fígado/imunologia , Células T Matadoras Naturais/imunologia , Zimosan/imunologia , Animais , Antígenos CD1d/imunologia , Interferon gama/biossíntese , Interferon gama/deficiência , Cinética , Macrófagos/imunologia , Camundongos , Camundongos Knockout , Receptor 2 Toll-Like/imunologia
12.
Biomed Res ; 32(3): 203-8, 2011 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-21673450

RESUMO

Balance between inflammatory and anti-inflammatory cytokines may be important in malaria presentation and outcome. To clarify cytokine interactions that produce pathology of malaria and control infection, C57BL/6 mice were infected with 10(4) parasitized RBCs from a non-lethal strain of Plasmodium yoelii. Kinetics was monitored showing the course of parasitemia, and cytokines were determined by RT-PCR from liver and spleen tissues. Inflammatory cytokines such as interferon-γ (IFNγ), interleukin (IL)-12, IL-6, tumor necrosis factor-α (TNFα) and anti-inflammatory cytokines, including IL-4 and IL-10, were investigated as key molecules that interact with immune cells in the activation of the immune responses. The production of IFNγ mRNA was found to be higher on day 7 than on day 21 after infection, and IL-12 and IL-6 showed higher expression in the liver than in the spleen. Though TNFα was highly expressed on day 14 after infection and on day 21 in the liver, such expression was decreased on day 21 in the spleen. Anti-inflammatory cytokines showed high expression in both the liver and spleen. The results suggest that a relative balance between inflammatory and anti-inflammatory cytokines is crucial and that the increase of inflammatory cytokine levels during the acute phase of malaria may reflect an early and effective immune response.The counteraction effect of anti-inflammatory cytokines is thought to play a role in limiting progression from uncomplicated malaria to severe life-threatening complications.


Assuntos
Citocinas/imunologia , Fígado/imunologia , Malária/imunologia , Plasmodium yoelii/imunologia , Baço/imunologia , Animais , Autoanticorpos/imunologia , Citocinas/sangue , Modelos Animais de Doenças , Eritrócitos/parasitologia , Hematócrito , Fígado/parasitologia , Malária/parasitologia , Camundongos , Camundongos Endogâmicos C57BL , Parasitemia/imunologia , RNA Mensageiro/análise , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Baço/parasitologia
13.
Immunology ; 133(1): 21-8, 2011 May.
Artigo em Inglês | MEDLINE | ID: mdl-21320121

RESUMO

Natural killer T (NKT) cells are known to be specifically activated by α-galactosylceramide (α-GalCer) via their interaction with CD1d. At that time, NKT cells mediate autoreactivity and eventually induce hepatic injury. As these immune responses resemble acute autoimmune hepatitis, it was examined whether autoantibody production and the activation of autoantibody-producing B-1 cells were accompanied by this phenomenon. Autoantibodies against Hep-2 cells and double-stranded DNA were detected in sera as early as day 3 (showing a peak at day 14) when mice were treated with α-GalCer. On day 3, B220(low) cells appeared in the liver. These B220(low) cells were CD5(-) (i.e. B-1b cells) and CD69(+) (an activation marker). Primarily, such B220(low) cells were present in the peritoneal cavity, but the proportion of B220(low) cells increased with the administration of α-GalCer even at this site. In parallel with the appearance of B220(low) cells in the liver, hepatic lymphocytes acquired the potential to produce autoantibodies in in vitro cell culture in the presence of lipopolysaccharide. These results suggested that hepatic injury induced by α-GalCer administration resembled acute autoimmune hepatitis and that the major effector lymphocytes were NKT cells with autoreactivity and autoantibody-producing B-1 cells.


Assuntos
Autoanticorpos/imunologia , Linfócitos B/imunologia , Doença Hepática Induzida por Substâncias e Drogas/imunologia , Galactosilceramidas/toxicidade , Células T Matadoras Naturais/imunologia , Animais , Autoanticorpos/biossíntese , Autoantígenos/imunologia , Subpopulações de Linfócitos B/imunologia , Ensaio de Imunoadsorção Enzimática , Feminino , Imunofluorescência , Galactosilceramidas/imunologia , Ativação Linfocitária/imunologia , Camundongos , Camundongos Endogâmicos C57BL
14.
J Investig Med ; 58(8): 968-70, 2010 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-20930645

RESUMO

The number of asthmatic patients is increasing in Japan. It is conceivable that changes in lifestyle (eg, lack of exercise and high-energy diet) may be associated with this phenomenon. The resulting factor seems to be altered activity of autonomic nervous system of these patients. When this activity was estimated by the measurement of heart rate variability, asthmatic patients (n = 11) showed a tendency for parasympathetic nerve dominance in comparison with healthy controls (n = 10). We recommend the patients engage in the "Senobi" stretch exercise, which involves stretching the arms and body upward while standing. After 1 month of regularly performing this exercise, most patients showed a decrease in the frequency of asthma rescue medication use. They also showed a recovery of forced expiratory volume in 1 second. These results suggest that the Senobi stretch is a useful exercise for asthmatic patients to perform to achieve a desirable improvement in symptoms.


Assuntos
Asma/terapia , Sistema Nervoso Autônomo/fisiopatologia , Exercícios Respiratórios , Terapia por Exercício , Asma/fisiopatologia , Feminino , Volume Expiratório Forçado/fisiologia , Frequência Cardíaca/fisiologia , Humanos , Masculino , Pessoa de Meia-Idade
15.
Biomed Res ; 31(2): 143-9, 2010 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-20460742

RESUMO

In this study, we examined the effect of the difference in time of going to sleep on the numerical values of leukocyte subsets and various hormones. Subjects consisted of 26 healthy adults (15 men, 11 women) with a mean age of 37.6 years. Among the 26 individuals, 12 persons (Group E) were of the habit of going to sleep before midnight consistently, while 14 persons (Group L) were of the habit of staying up late, consistently going to sleep after 2 am. For Group E, it was found that the ratio of lymphocytes was remarkably high in comparison with Group L (Group E 41.6 +/- 2.54%, Group L 31.7 +/- 2.03%, P < 0.01). On the other hand, for Group L it was found that the ratio of granulocytes was remarkably high in comparison with Group E (Group E 53.0 +/- 2.51%, Group L 62.3 +/- 2.22%, P < 0.01). However, no difference was observed in lymphocyte and granulocyte ratios due to the duration of the sleep. As the excessive quantity of granulocytes was not corrected through longer sleep, these findings suggest that the time when first going to sleep is more important than the total hours of sleep achieved.


Assuntos
Granulócitos/imunologia , Hábitos , Linfócitos/imunologia , Sono , Adulto , Feminino , Humanos , Leucócitos , Contagem de Linfócitos , Masculino
16.
Cell Immunol ; 263(1): 49-54, 2010.
Artigo em Inglês | MEDLINE | ID: mdl-20231018

RESUMO

Mice with malaria showed unique immunological responses, including the expansion of NK1.1(-)TCR(int) cells (extrathymic T cells). Since TCR(int) cells with autoreactivity and autoantibody-producing B cells (B-1 cells) are often simultaneously activated under autoimmune conditions, it was examined whether B-1 cells were activated in the course of malarial infection. From days 14 after infection, B220(low) B-1 cells appeared in the liver and spleen. The number of B220(low) B cells was highest at day 14, but the ratio was highest at days 28-35. In parallel with the appearance of B220(low) cells, autoantibodies against HEp-2 cells and double-stranded DNA were detected in sera. These B220(low) cells had phenotypes of CD44(high), CD23(-) and CD62L(-). In sharp contrast, conventional B220(high) B cells (B-2 cells) were CD44(low), CD23(+) and CD62L(+). These results suggested that malaria immune responses were not mediated by conventional T and B cells but resembled the responses during autoimmune diseases.


Assuntos
Anticorpos Antinucleares/metabolismo , Subpopulações de Linfócitos B/metabolismo , Linfócitos B/metabolismo , Malária/imunologia , Plasmodium/imunologia , Animais , Antígenos CD/biossíntese , Subpopulações de Linfócitos B/imunologia , Subpopulações de Linfócitos B/parasitologia , Subpopulações de Linfócitos B/patologia , Linfócitos B/imunologia , Linfócitos B/parasitologia , Linfócitos B/patologia , Células Hep G2 , Humanos , Antígenos Comuns de Leucócito/biossíntese , Fígado/metabolismo , Fígado/patologia , Malária/patologia , Malária/fisiopatologia , Camundongos , Camundongos Endogâmicos C57BL , Parasitemia , Plasmodium/patogenicidade , Baço/patologia , Transaminases/metabolismo
17.
Cell Immunol ; 260(2): 105-12, 2010.
Artigo em Inglês | MEDLINE | ID: mdl-19857863

RESUMO

Severe hepatic injury is induced by Concanavalin A (Con A) administration in mice, the major effector cells being CD4(+) T cells, NKT cells and macrophages. Since autologous lymphocyte subsets are associated with tissue damage, Con A-induced hepatic injury is considered to be autoimmune hepatitis. However, it has remained to be investigated how autoantibodies and B-1 cells are responsible for this phenomenon. In this study, it was demonstrated that autoantibodies which were detected using Hep-2 cells in immunofluorescence tests and using double-strand (ds) DNA in the ELISA method, appeared after Con A administration (a peak at day 14). Moreover, autoantibody-producing B220(low) cells (i.e., B-1 cells) also appeared at this time. Purified B220(low) cells were found to have a potential to produce autoantibodies. These results suggest that Con A-induced hepatic injury indeed includes the mechanism of autoimmune hepatitis.


Assuntos
Autoanticorpos/imunologia , Linfócitos B/imunologia , Hepatite Animal/imunologia , Células T Matadoras Naturais/imunologia , Alanina Transaminase/sangue , Animais , Autoanticorpos/sangue , Linfócitos B/citologia , Linhagem Celular Tumoral , Concanavalina A , Ensaio de Imunoadsorção Enzimática , Feminino , Granulócitos/citologia , Granulócitos/imunologia , Hepatite Animal/sangue , Hepatite Animal/induzido quimicamente , Humanos , Imuno-Histoquímica , Contagem de Linfócitos , Macrófagos/citologia , Macrófagos/imunologia , Camundongos , Camundongos Endogâmicos C57BL , Células T Matadoras Naturais/citologia , Baço/citologia , Baço/imunologia , Fatores de Tempo
18.
Biomed Res ; 30(3): 157-63, 2009 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-19574716

RESUMO

The effect of acute stress on the immune system was examined in mice. Restraint stress decreased the number of lymphocytes in the liver, whereas the number of lymphocytes remained unchanged in the spleen and thymus. In the liver, the decrease in number appeared at 1.5 h and fell to a third of he control level at 3 h. The proportions of IL-2Rbeta(+)CD3(int) cells, NKT cells, CD44(+) T cells and B cells were changed in the liver. The absolute numbers of IL-2Rbeta(+)CD3(int) cells, NKT cells and CD3(+)CD44(+) cells remained constant in the liver under the stress, while those of total T cells and NK cells decreased. The levels of hyaluronan (HA) in various tissues and sera were then examined. The expression of hyaluronan binding protein (HABP) was found to increase in the skin, liver and kidney as shown by immunohistochemical staining. An increase of HA in sera due to stress was seen at 3 h. The present results suggest that the activation of CD44(+) T cells and unconventional T cells (i.e., innate immunity) in the blood and the elevated levels of HA (ligand for CD44) in the tissues and blood are crucial responses to acute stress exposure.


Assuntos
Ácido Hialurônico/metabolismo , Imunidade Inata/fisiologia , Fígado/imunologia , Restrição Física/psicologia , Estresse Fisiológico/imunologia , Estresse Psicológico/imunologia , Animais , Separação Celular , Citometria de Fluxo , Regulação da Expressão Gênica , Glucuronosiltransferase/genética , Glucuronosiltransferase/metabolismo , Humanos , Receptores de Hialuronatos/genética , Receptores de Hialuronatos/metabolismo , Hialuronan Sintases , Subpopulações de Linfócitos/citologia , Subpopulações de Linfócitos/imunologia , Linfócitos/citologia , Linfócitos/imunologia , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Distribuição Tecidual
19.
Int J Hematol ; 90(2): 217-225, 2009 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-19639271

RESUMO

We report seven large B cell lymphoma patients showing the involvement of tumor cells with cyclin D3 (CCND3) expression in bone marrow (BM) at the initial diagnosis. All patients presented with B symptoms, splenomegaly, and anemia/thrombocytopenia lacking hemophagocytosis in the BM. Five of the seven patients had suffered from immunological diseases or cancers. The tumor cells were divided into those with a lymphoplasmacytoid or blastoid appearance. Six cases were confirmed to express CD5 antigen on tumor cells. Three cases presented a chromosomal translocation between CCND3 and the immunoglobulin heavy chain (IGH) loci, t(6;14)(p21;q32). Three and two cases showed unmutated and mutated sequences of the variable region of IGH (VH), respectively, and one case showed deletion of an entire segment of VH. Two cases with t(6;14)(p21;q32) showed an unmutated VH sequence and chromosomal translocation within the switch region of IGH. Further studies are required to determine whether CCND3 expression is associated with a unique subset of diffuse large B cell lymphoma.


Assuntos
Linfócitos B/fisiologia , Células da Medula Óssea/fisiologia , Ciclinas/genética , Genótipo , Linfoma Difuso de Grandes Células B/genética , Idoso , Idoso de 80 Anos ou mais , Linfócitos B/patologia , Células da Medula Óssea/patologia , Ciclina D3 , Feminino , Regulação Neoplásica da Expressão Gênica , Humanos , Região de Troca de Imunoglobulinas/genética , Imunofenotipagem , Linfoma Difuso de Grandes Células B/imunologia , Linfoma Difuso de Grandes Células B/patologia , Masculino , Pessoa de Meia-Idade , Translocação Genética
20.
Cell Immunol ; 259(1): 66-73, 2009.
Artigo em Inglês | MEDLINE | ID: mdl-19595299

RESUMO

Mice were exposed to starvation for 3 days. Body temperature and various parameters were examined. By starvation, body temperature, blood glucose and ACTH decreased, especially on days 2 and 3. The level of corticosterone increased at this time. On the other hand, the number of lymphocytes yielded by the liver, spleen and thymus decreased from day 1 to 3. The change of the distribution of lymphocyte subsets was unique because NK, NKT and extrathymic T cells were stress-resistant in the liver. Conventional T and B cells were stress-sensitive. Reflecting the increased proportion of NK and NKT cells, NK and NKT activities were augmented. The increased proportion of NKT cells produced both IFNgamma and IL-4 (Th0-type profile). The proportion and some functions of granulocytes and macrophages increased on Day 1 after starvation. These results suggest that starvation has a potential to increase the functions of unconventional lymphocytes and myeloid cells.


Assuntos
Imunidade Inata , Células Matadoras Naturais/imunologia , Células T Matadoras Naturais/imunologia , Inanição/imunologia , Estresse Fisiológico/imunologia , Subpopulações de Linfócitos T/imunologia , Hormônio Adrenocorticotrópico/sangue , Animais , Glicemia/análise , Temperatura Corporal/fisiologia , Corticosterona/sangue , Citotoxicidade Imunológica/imunologia , Granulócitos/imunologia , Granulócitos/metabolismo , Interferon gama/imunologia , Interferon gama/metabolismo , Subunidade p35 da Interleucina-12/imunologia , Subunidade p35 da Interleucina-12/metabolismo , Subunidade p40 da Interleucina-12/imunologia , Subunidade p40 da Interleucina-12/metabolismo , Células Matadoras Naturais/metabolismo , Fígado/imunologia , Fígado/metabolismo , Fígado/patologia , Camundongos , Camundongos Endogâmicos C57BL , Células T Matadoras Naturais/metabolismo , Baço/imunologia , Baço/metabolismo , Baço/patologia , Superóxidos/metabolismo , Timo/imunologia , Timo/metabolismo , Timo/patologia , Fator de Necrose Tumoral alfa/imunologia , Fator de Necrose Tumoral alfa/metabolismo
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA