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1.
Gene ; 576(1 Pt 2): 358-65, 2016 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-26526133

RESUMO

Phaseolus vulgaris is subjected to serious post-harvest diseases such as grey mold and cottony rot diseases caused by Botrytis cinerea and Pythium aphanidermatum, respectively. In current study, potassium silicate (KSi), potassium thiosulfate (KTS) and potassium sulfate (KS) suppressed moderately the growth of B. cinerea and P. aphanidermatum in vitro. The applied treatments significantly suppressed grey mold and cottony rot of Xera and Valentino snap beans varieties' pods stored at 7 ± 1°C and 90-95% RH for 20 days. Ethylene responsive factor (ERF), polygalacturonase inhibitor protein (PGIP), phosphatase associated to defense (PA) and pathogenesis-related protein (PR1) defense genes were over-expressed in leaves tissue of both bean varieties responding positively to potassium salts field application. The expression of these genes was influenced by plant genotype and environment as it varied by snap bean varieties. Accumulation of ERF, GIP, PA and PR1 genes transcript under KTS at 4000 ppm treatment were the highest in Xera tissues (3.5-, 4.8-, 4- and 4.8-fold, respectively). In conclusion, pre-harvest potassium salt in vivo application could be used as effective safe alternatives to fungicides against grey mold and cottony rot diseases of snap beans during storage for up to 20 days at 7 ± 1°C.


Assuntos
Botrytis/efeitos dos fármacos , Phaseolus/genética , Pythium/efeitos dos fármacos , Sulfatos/farmacologia , Tiossulfatos/farmacologia , Agricultura/métodos , Botrytis/patogenicidade , Resistência à Doença/genética , Fungicidas Industriais/farmacologia , Regulação da Expressão Gênica de Plantas , Phaseolus/efeitos dos fármacos , Phaseolus/metabolismo , Doenças das Plantas/genética , Doenças das Plantas/microbiologia , Doenças das Plantas/prevenção & controle , Folhas de Planta/efeitos dos fármacos , Folhas de Planta/genética , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Potássio/farmacologia , Pythium/patogenicidade , Silicatos/farmacologia
2.
GM Crops ; 1(4): 250-6, 2010.
Artigo em Inglês | MEDLINE | ID: mdl-21844680

RESUMO

The accumulation of compatible solutes during stress in plant cell is well documented. Proline is one of these solutes which accumulate in the cytosol in response to drought or salinity stress in plants. Proline has several functions during stress just like osmotic adjustment, osmoprotection, free radical scavenger and antioxidant. Ornithine δ-aminotransferase (δ-OAT) is an important enzyme in proline biosynthetic pathway. It catalyzes the transamination of ornithine to pyrroline-5-carboxylate which can be reduced into proline. Expression of ornithine δ-aminotransferase gene isolated from Vicia villosa (VvOAT) showed protein with a molecular mass of 63 KDa which is compatible with the predicted mass and after VvOAT gene delivery into E. coli host HB101, VvOAT gene enhanced its salt tolerance. Homology modeling of VvOAT was performed based on the crystal structure of the ornithine δ-aminotransferase from humans (PDB code 2OATA). With this model, a flexible docking study with the substrate and inhibitors was performed. The results indicated that PHE170 and ASN171 in VvOAT are the important determinant residues in binding as they have strong hydrogen bonding contacts with the substrate and inhibitors. All the obtained results indicated the efficiency of utilizing this gene in conferring salt tolerance.


Assuntos
Modelos Moleculares , Ornitina-Oxo-Ácido Transaminase/química , Proteínas de Plantas/química , Vicia/enzimologia , Sequência de Aminoácidos , Asparagina/química , Asparagina/genética , Asparagina/metabolismo , Sítios de Ligação/genética , Eletroforese em Gel de Ágar , Eletroforese em Gel de Poliacrilamida , Escherichia coli/genética , Regulação Enzimológica da Expressão Gênica , Humanos , Dados de Sequência Molecular , Ornitina/química , Ornitina/metabolismo , Ornitina-Oxo-Ácido Transaminase/genética , Ornitina-Oxo-Ácido Transaminase/metabolismo , Fenilalanina/química , Fenilalanina/genética , Fenilalanina/metabolismo , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Ligação Proteica , Conformação Proteica , Proteínas Recombinantes/química , Proteínas Recombinantes/metabolismo , Homologia de Sequência de Aminoácidos , Especificidade por Substrato , Vicia/genética
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