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1.
Anal Chim Acta ; 1178: 338809, 2021 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-34482865

RESUMO

We present a new analytical approach for the analysis of triacylglycerol fatty acyls distribution by normal phase liquid chromatography (NPLC) coupled with APPI+-HRMS. The NPLC method used allows the separation of more than 30 classes of lipids. The energy of the APPI+ source enables the formation of low-intensity ions B fragments ([RC = O+74]+ <3%), characteristic of lipids with a glycerol esterified by one or more fatty acyls. We found the relative intensities of ions B were close to the fatty acyl distribution. To establish the proof of concept, we decided to focus on the triacylglycerols (TGs) class, the major component of plant oils. By either NPLC or FIA, the TGs class appeared as a single peak. In our experimental conditions, ions B are always present in the mass spectra of TGs and each ion B is specific to a fatty acyl group. The Orbitrap mass spectrometer featured high enough resolution and accuracy to identify ions B and distinguish them from other TG fragment ions. A further adjustment of the fatty acyls relative quantities calculation from ions B intensities was computed using weighting coefficients of ions B response. The methodology was developed and validated using plant oils characterized by a GC-FID reference method. NPLC-APPI+-HRMS method offers the advantage of analyzing the fatty acyl composition of complex lipid extracts without the need for sample preparation.


Assuntos
Pressão Atmosférica , Monoglicerídeos , Cromatografia Líquida de Alta Pressão , Lipídeos , Espectrometria de Massas , Triglicerídeos
2.
Front Microbiol ; 11: 1399, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32655536

RESUMO

In condition of over-expression, SCO3201, a regulator of the TetR family was previously shown to strongly inhibit antibiotic production and morphological differentiation in Streptomyces coelicolor M145. In order to elucidate the molecular processes underlying this interesting, but poorly understood phenomenon, a comparative analysis of the lipidomes and transcriptomes of the strain over-expressing sco3201 and of the control strain containing the empty plasmid, was carried out. This study revealed that the strain over-expressing sco3201 had a higher triacylglycerol content and a lower phospholipids content than the control strain. This was correlated with up- and down- regulation of some genes involved in fatty acids biosynthesis (fab) and degradation (fad) respectively, indicating a direct or indirect control of the expression of these genes by SCO3201. In some instances, indirect control might involve TetR regulators, whose encoding genes present in close vicinity of genes involved in lipid metabolism, were shown to be differentially expressed in the two strains. Direct interaction of purified His6-SCO3201 with the promoter regions of four of such TetR regulators encoding genes (sco0116, sco0430, sco4167, and sco6792) was demonstrated. Furthermore, fasR (sco2386), encoding the activator of the main fatty acid biosynthetic operon, sco2386-sco2390, has been shown to be an illegitimate positive regulatory target of SCO3201. Altogether our data demonstrated that the sco3201 over-expressing strain accumulates TAG and suggested that degradation of fatty acids was reduced in this strain. This is expected to result into a reduced acetyl-CoA availability that would impair antibiotic biosynthesis either directly or indirectly.

3.
Antibiotics (Basel) ; 9(6)2020 May 26.
Artigo em Inglês | MEDLINE | ID: mdl-32466356

RESUMO

Streptomycetes are well known antibiotic producers and are among the rare prokaryotes able to store carbon as lipids. Previous comparative studies of the weak antibiotic producer Streptomyces lividans with its ppk mutant and with Streptomyces coelicolor, which both produce antibiotics, suggested the existence of a negative correlation between total lipid content and the ability to produce antibiotics. To determine whether such a negative correlation can be generalized to other Streptomyces species, fifty-four strains were picked randomly and grown on modified R2YE medium, limited in phosphate, with glucose or glycerol as the main carbon source. The total lipid content and antibiotic activity against Micrococcus luteus were assessed for each strain. This study revealed that the ability to accumulate lipids was not evenly distributed among strains and that glycerol was more lipogenic than glucose and had a negative impact on antibiotic biosynthesis. Furthermore, a statistically significant negative Pearson correlation between lipid content and antibiotic activity could be established for most strains, but a few strains escape this general law. These exceptions are likely due to limits and biases linked to the type of test used to determine antibiotic activity, which relies exclusively on Micrococcus luteus sensitivity. They are characterized either by high lipid content and high antibiotic activity or by low lipid content and undetectable antibiotic activity against Micrococcus luteus. Lastly, the comparative genomic analysis of two strains with contrasting lipid content, and both named Streptomyces antibioticus (DSM 41,481 and DSM 40,868, which we found to be phylogenetically related to Streptomyces lavenduligriseus), indicated that some genetic differences in various pathways related to the generation/consumption of acetylCoA could be responsible for such a difference.

4.
Mol Pharm ; 16(9): 4045-4058, 2019 09 03.
Artigo em Inglês | MEDLINE | ID: mdl-31361499

RESUMO

Retinoblastoma is a malignant tumor of the retina in infants. Conventional therapies are associated to severe side effects and some of them induce secondary tumors. Photodynamic therapy (PDT) thus appears as a promising alternative as it is nonmutagenic and generates minimal side effects. The effectiveness of PDT requires the accumulation of a photosensitizer (PS) in the tumor. However, most porphyrins are hydrophobic and aggregate in aqueous medium. Their incorporation into a nanocarrier may improve their delivery to the cell cytoplasm. In this work, we designed biodegradable liponanoparticles (LNPs) consisting of a poly(d,l)-lactide (PDLLA) nanoparticle coated with a phospholipid (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine/1,2-dioleoyl-3-trimethylammonium-propane) bilayer. An anticancer drug, beta-lapachone (ß-Lap) and a PS, m-THPC, were co-encapsulated for combined chemo- and PDT because it has been suggested that they may have a synergistic effect based on the activation of ß-Lap by PDT-induced over-expression of the enzyme NQO1. Using dynamic light scattering measurements, cryogenic transmission electron microscopy, and fluorescence confocal microscopy, we selected the appropriate conditions for the encapsulation of the compounds. LNPs were internalized in retinoblastoma cells within few hours. No obvious synergistic effect related to the activation of ß-Lap by PDT was observed. Conversely, the LNPs were cytotoxic at lower doses of the two encapsulated compounds as compared to the single therapies. Analysis of the combinatorial treatment showed that PDT and chemotherapy had an additive effect on the viability of retinoblastoma cells.


Assuntos
Ácidos Graxos Monoinsaturados/química , Mesoporfirinas/química , Nanopartículas/química , Naftoquinonas/química , Fosfatidilcolinas/química , Fotoquimioterapia/métodos , Poliésteres/química , Compostos de Amônio Quaternário/química , Retinoblastoma/tratamento farmacológico , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Microscopia Crioeletrônica , Composição de Medicamentos/métodos , Sistemas de Liberação de Medicamentos/métodos , Estabilidade de Medicamentos , Difusão Dinâmica da Luz , Humanos , Microscopia Confocal , Fármacos Fotossensibilizantes/química , Retinoblastoma/patologia
5.
Mol Pharm ; 16(7): 2999-3010, 2019 07 01.
Artigo em Inglês | MEDLINE | ID: mdl-31117740

RESUMO

The encapsulation of glucocorticoids, such as dexamethasone, in nanoparticles (NPs) faces two main issues: a low drug loading and the destabilization of the nanoparticle suspension due to drug crystallization. Here, we successfully formulated a prodrug of dexamethasone, dexamethasone palmitate (DXP), into nanoparticles stabilized by the sole presence of distearoyl- sn-glycero-3-phosphoethanolamine- N-[methoxy(poly(ethylene glycol))-2000] (DSPE-PEG2000). Two formulation processes, nanoprecipitation and emulsion-evaporation, allowed the formation of stable nanoparticles. By adjusting the drug/lipid ratio and the DXP concentration, nanoparticles of DXP (DXP-NPs) with a size between 130 and 300 nm can be obtained. Owing to the presence of DSPE-PEG2000, a high drug entrapment efficiency of 98% w/w was reached for both processes, corresponding to a very high equivalent dexamethasone drug loading of around 50% w/w in the absence of crystallization upon storage at 4 °C. The anti-inflammatory activity of DXP-NPs was preserved when incubated with macrophages activated with lipopolysaccharide. Pharmacokinetics parameters were evaluated after intravenous (IV) injection of DXP-NPs to healthy mice. The release of DXM from DXP-NPs in plasma was clearly controlled up to 18 h compared with the free drug, which was rapidly eliminated from plasma after administration. In conclusion, a novel type of nanoparticle combining the advantages of prodrugs and nanoparticles was designed, easy to produce with a high loading efficiency and leading to modified pharmacokinetics and tissue distribution after IV administration.


Assuntos
Anti-Inflamatórios/farmacocinética , Dexametasona/farmacocinética , Composição de Medicamentos/métodos , Sistemas de Liberação de Medicamentos/métodos , Nanopartículas/química , Pró-Fármacos/farmacocinética , Animais , Anti-Inflamatórios/química , Sobrevivência Celular/efeitos dos fármacos , Cristalização , Dexametasona/administração & dosagem , Dexametasona/química , Liberação Controlada de Fármacos , Estabilidade de Medicamentos , Injeções Intravenosas , Masculino , Camundongos , Camundongos Endogâmicos DBA , Nanopartículas/administração & dosagem , Tamanho da Partícula , Fosfatidiletanolaminas/administração & dosagem , Fosfatidiletanolaminas/química , Polietilenoglicóis/administração & dosagem , Polietilenoglicóis/química , Pró-Fármacos/administração & dosagem , Pró-Fármacos/química , Células RAW 264.7 , Distribuição Tecidual
6.
J Control Release ; 296: 179-189, 2019 02 28.
Artigo em Inglês | MEDLINE | ID: mdl-30659904

RESUMO

Rheumatoid arthritis (RA) is a prevalent autoimmune disease characterized by joint inflammation, bone and cartilage erosion. The use of glucocorticoids in the treatment of RA is hampered by significant side effects induced by their unfavorable pharmacokinetics. Delivering glucocorticoids by means of nanotechnologies is promising but the encapsulation of highly crystalline and poorly water-soluble drugs results in poor loading and low stability. We report here the design of 130 nm nanoparticles made of solely dexamethasone palmitate, stabilized by polyethylene glycol-linked phospholipids displaying a negative zeta potential (-55 mV), high entrapment efficiency and stability over 21 days under storage at 4 °C. X ray diffraction showed no crystallization of the drug. When incubated in serum, nanoparticles released free dexamethasone which explains the in vitro anti-inflammatory effect on LPS-activated RAW 264.7 macrophages. Moreover, we demonstrate in a murine collagen-induced arthritis model the improved therapeutic efficacy of these nanoparticles. Their passive accumulation in arthritic joints leads to disease remission and recovery of the joint structure at a dose of 1 mg/kg dexamethasone, without any adverse effects. Dexamethasone palmitate nanoparticles are promising in the treatment of inflammation in rheumatoid arthritis with a very significant difference occurring at the late stage of inflammation allowing to prevent the progression of the disease.


Assuntos
Anti-Inflamatórios/administração & dosagem , Artrite Experimental/tratamento farmacológico , Artrite Reumatoide/tratamento farmacológico , Dexametasona/administração & dosagem , Portadores de Fármacos/administração & dosagem , Nanopartículas/administração & dosagem , Palmitatos/administração & dosagem , Animais , Artrite Experimental/patologia , Artrite Reumatoide/patologia , Articulações/efeitos dos fármacos , Articulações/patologia , Masculino , Camundongos , Camundongos Endogâmicos DBA , Células RAW 264.7
7.
Med. interna (Caracas) ; 34(2): 128-132, 2018. ilus, tab
Artigo em Espanhol | LILACS, LIVECS | ID: biblio-1007866

RESUMO

Existe una asociación epidemiológica entre sífilis e infección por VIH; las úlceras genitales favorecen la transmisión de Treponema pallidum y éste a su vez, la transmisión del VIH. La dermatitis seborreica (DS) se presenta en 2 a 4% de la población general; sin embargo, en los pacientes con infección por VIH/SIDA y SIFILIS es significativamente mayor, llegando a 85% en algunas series. Se presenta el caso de un paciente masculino de 22 años de edad, natural y procedente del Distrito Capital, Venezuela, sin otras patologías conocidas, quien acude a la emergencia por presentar periodos de hetero agresividad y desorientación témporo ­ espacial., El familiar refiere aparición de lesiones descamativas de fondo eritematoso en región facial desde hace 6 meses, no pruriginosas y a la exploración física se evidencia parafasia y pupila de Argyll- Robertson. Se reportan serologías Positivas para HIV por ELISA de 4ta Generación, VDRL Reactivo a 4 diluciones y FTA-ABS Reactivo, La punción Lumbar evidenció pleocitosis, hiperproteinorraquia, hipoglucorraquia y VDRL REACTIVO. La biopsia cutánea con coloración argéntica de Fontana fué positiva para Treponema pallidum, con mejoría clínica significativa posterior al cumplimiento del esquema de Penicilina Cristalina. La dermatitis seborreica puede ser uno de los primeros indicadores de infección por VIH, por lo cual en toda dermatitis seborreica atípica, extensa o que no responda a tratamiento debe solicitarse serología para VIH y VDRL(AU)


There is an epidemiological association between syphilis and HIV infection; genital ulcers facilitatethe transmission of Treponema pallidum and this in turn, the transmission of HIV. Seborrheic dermatitis (SD) occurs in 2 to 4% of the general population; however, in patients with HIV / AIDS and SIFILIS infection it is significantly higher, reaching 85% in some series. We present the case of a male patient, of a 22 years-old , natural from the Caracas, Venezuela, without other known pathologies, who came to the emergency ward due to periods of aggressiveness and temporo - spatial disorientation. Desquamative lesions with an erythematous background in in his face appeared 6 months earlier.The neurological examination showeds paraphasia and Argyll-Robertson´ pupil. Positive serologies for HIV by 4th Generation ELISA and VDRL reactive at 4 dilutions as well as positive FTA-ABS were reported, Lumbar tab evidenced pleocytosis, hyperproteinorrachia, hypoglucorraquia and reagent VDRL . The skin biopsy with Fontana silver coloration was positive for Treponema pallidum and he improved clinically after treatment with a Crystalline Penicillin scheme. Seborrheic dermatitis may be one of the first indicators of HIV infection, so in all atypical seborrheic dermatitis, extensive or unresponsive to treatment, serology for HIV and VDRL should be determined(AU)


Assuntos
Humanos , Masculino , Adulto , Treponema pallidum , Sífilis/fisiopatologia , HIV/efeitos dos fármacos , Dermatite Seborreica/fisiopatologia , Epidemiologia , Medicina Interna
8.
J Chromatogr A ; 1514: 54-71, 2017 Sep 08.
Artigo em Inglês | MEDLINE | ID: mdl-28774713

RESUMO

One important challenge in lipid class analysis is to develop a method suitable or, at least adaptable, for a vast diversity of samples. In the current study, an improved normal-phase liquid chromatography (NPLC) method allowed analyzing the lipid classes present in mammalian, vegetable as well as microorganism (yeast and bacteria) lipid samples. The method effectively separated 30 lipid classes or subclasses with a special focus on medium polarity lipids. The separation was carried out with bare silica stationary phase and was coupled to evaporative light scattering detection (ELSD), charged aerosol detection (Corona-CAD®) and mass spectrometry. Solutions are provided to circumvent technical issues (such as pumping solvents of low viscosity, solvent purity, rinsing step). The influence of mobile phase composition and addition of ionic modifiers on the chromatographic behavior of particular lipid classes is documented. A comparison between ELSD and Corona-CAD® confirmed the interest of this later detector for samples with a wide range of concentration of different lipids. Three common atmospheric pressure ionization interfaces were used for coupling the NPLC separation to a LTQ Velos Pro® mass spectrometer. The comparison of the chromatographic profiles showed that atmospheric pressure chemical ionization (APCI) and atmospheric pressure photoionization (APPI) are both suitable to detect the different lipid classes whereas APPI allows a better sensitivity for lipids at low-concentration.


Assuntos
Cromatografia Líquida de Alta Pressão/métodos , Lipídeos/análise , Espectrometria de Massas por Ionização por Electrospray , Aerossóis/química , Animais , Pressão Atmosférica , Encéfalo/metabolismo , Bovinos , Galinhas , Gema de Ovo/química , Gema de Ovo/metabolismo , Escherichia coli/química , Escherichia coli/metabolismo , Íons/química , Lipídeos/química , Fígado/química , Fígado/metabolismo , Miocárdio/química , Miocárdio/metabolismo , Saccharomyces cerevisiae/química , Saccharomyces cerevisiae/metabolismo , Solventes/química , Soja/química , Soja/metabolismo
9.
J Cell Biochem ; 118(10): 3480-3487, 2017 10.
Artigo em Inglês | MEDLINE | ID: mdl-28345768

RESUMO

Transplant vasculopathy may be considered as an accelerated form of atherosclerosis resulting in chronic rejection of vascularized allografts. After organ transplantation, a diffuse intimal thickening is observed, leading to the development of an atherosclerosis plaque due to a significant monocyte infiltration. This results from a chronic inflammatory process induced by the immune response. In this study, we investigated the impact of two immunosuppressive drugs used in therapy initiated after organ transplantation, mycophenolate mofetil, and rapamycin, on the apoptotic response of monocytes induced or not by oxidized LDL. Here we show the pro-apoptotic effect of these two drugs through two distinct signaling pathways and we highlight a synergistic effect of rapamycin on apoptosis induced by oxidized LDL. In conclusion, since immunosuppressive therapy using mycophenolate mofetil or rapamycin can increase the cell death in a monocyte cell line, this treatment could exert similar effects on human monocytes in transplant patients, and thus, prevent transplant vasculopathy, atherosclerosis development, and chronic allograft rejection. J. Cell. Biochem. 118: 3480-3487, 2017. © 2017 Wiley Periodicals, Inc.


Assuntos
Apoptose/efeitos dos fármacos , Monócitos/metabolismo , Ácido Micofenólico/farmacologia , Transdução de Sinais/efeitos dos fármacos , Sirolimo/farmacologia , Humanos , Lipoproteínas LDL/farmacologia , Células U937
10.
J Chromatogr A ; 1464: 55-63, 2016 Sep 16.
Artigo em Inglês | MEDLINE | ID: mdl-27554026

RESUMO

An evaluation of solvents alternative to n-heptane (d-limonene and hexamethyldisiloxane) and chloroform (cyclopentyl methyl ether, 2-methyltetrahydrofuran and isopentyl acetate) was developed for lipid classes separation of non-polar cholesteryl ester to highly polar phospholipids by high-performance liquid chromatography on bare silica stationary phase and evaporative light-scattering detection. Screening of alternative solvents was used to estimate their compatibility with liquid chromatography and evaporative light-scattering detection and to evaluate their chromatographic selectivity. This work shows that n-heptane can be advantageously replaced by hexamethyldisiloxane. An increase of non-polar lipids retention is observed with hexamethyldisiloxane as weak solvent. Chloroform, which is largely used for lipid analysis, might be replaced efficaciously by cyclopentyl methyl ether, 2-methyltetrahydrofuran or isopentyl acetate. Aside from offering a different selectivity, the gradients composed by one or both alternative solvents gave efficient and comparable or even better separations than those obtained with conventional solvents.


Assuntos
Lipídeos/química , Cromatografia Líquida de Alta Pressão/instrumentação , Cromatografia Líquida de Alta Pressão/métodos , Estrutura Molecular
11.
Rev. Esc. Enferm. USP ; 48(6): 1111-1118, 12/2014. tab, graf
Artigo em Inglês | LILACS, BDENF - Enfermagem | ID: lil-736327

RESUMO

Objective To analyze the production of scientific knowledge about the use of patients’ classification instruments in care and management practice in Brazil. Method Integrative literature review with databases search in: Latin American and Caribbean Literature on Health Sciences (LILACS), Medical Literature Analysis and Retrieval System on-line (MEDLINE), Cumulative Index to Nursing and Allied Health Literature (CINAHL) and SCOPUS, between January 2002 through December 2013. Results 1,194 studies were found, 31 met the inclusion criteria. We observed a higher number of studies in the category care plans and workload (n=15), followed by the category evaluation of psychometric properties (n=14). Conclusion Brazilian knowledge production has not yet investigated some purposes of using instruments for classifying patients in professional nursing practice. The identification of unexplored areas can guide future research on the topic.
 .


Objective To analyze the production of scientific knowledge about the use of patients’ classification instruments in care and management practice in Brazil. Method Integrative literature review with databases search in: Latin American and Caribbean Literature on Health Sciences (LILACS), Medical Literature Analysis and Retrieval System on-line (MEDLINE), Cumulative Index to Nursing and Allied Health Literature (CINAHL) and SCOPUS, between January 2002 through December 2013. Results 1,194 studies were found, 31 met the inclusion criteria. We observed a higher number of studies in the category care plans and workload (n=15), followed by the category evaluation of psychometric properties (n=14). Conclusion Brazilian knowledge production has not yet investigated some purposes of using instruments for classifying patients in professional nursing practice. The identification of unexplored areas can guide future research on the topic.

 .


Objetivo Analizar la producción del conocimiento científico acerca de la utilización de instrumentos de clasificación de pacientes en la práctica asistencial y de gestión en Brasil. Método Revisión integradora de la literatura con consulta a las bases de datos: Literatura Latinoamericana y del Caribe en Ciencias de la Salud (LILACS), Medical Literature Analysis and Retrieval System on-line (MEDLINE), Cumulative Index to Nursing and Allied Health Literature (CINAHL) y SCOPUS, relativas al período de enero de 2002 a diciembre de 2013. Resultados De las 1.194 publicaciones encontradas, 31 atendieron a los criterios de selección. Se observó una mayor cantidad de artículos en la categoría perfil asistencial y carga laboral (n=15), seguidos de la categoría evaluación de las propiedades psicométricas (n=14). Conclusión La producción nacional todavía no ha investigado algunas finalidades de utilización de instrumentos de clasificación de pacientes en la práctica profesional del enfermero. La identificación de áreas aún no exploradas podrá orientar futuras investigaciones acerca de la temática. .


Assuntos
Humanos , Bibliometria , Processo de Enfermagem/estatística & dados numéricos , Brasil , Avaliação em Enfermagem/estatística & dados numéricos , Psicometria , Reprodutibilidade dos Testes
12.
Psicol. reflex. crit ; 27(1): 171-178, jan.-mar. 2014. tab
Artigo em Inglês | LILACS | ID: lil-709999

RESUMO

The purpose of this study is to analyze how family systems influence adolescents' suicidal ideation. The participants were 534 adolescents (51.1% female and 48.9% male), with ages ranging from 14 to 18 years (M=16.18; SD=1.14). The instruments used were the following: Family Adaptability and Cohesion Evaluation Scale, Psychological Separation Inventory and Questionnaire of Suicidal Ideation. Results have shown that adolescents with higher suicidal ideation belong to the so-called unbalanced families (with low cohesion and flexibility) and have a conflictual dependence to their parents. On the other hand, adolescents that belong to balanced families revealed lower suicidal ideation. In conclusion, the family's cohesion and flexibility are protective factors against adolescents' suicidal ideation.


O objetivo deste estudo é analisar de que forma o funcionamento familiar influencia a ideação suicida na adolescência. Para tal, analisámos a coesão e adaptabilidade familiar, assim como a independência conflitual. Participaram neste estudo 534 adolescentes (51,1% raparigas e 48,9% rapazes), com idades compreendidas entre 14 e 18 anos (M=16,18; DP=1,14). Os instrumentos utilizados foram: Escala de Adaptabilidade e Coesão Familiar; Inventário Separação Psicológica e Questionário Ideação Suicida. Os resultados mostraram que os adolescentes com maior ideação suicida pertencem a famílias desequilibradas (coesão e adaptabilidade baixa) e têm uma forte dependência conflitual a ambos os pais. Os adolescentes pertencentes a famílias equilibradas revelaram menor ideação suicida. A coesão e a capacidade adaptativa da família são fatores protetores da ideação suicida.


Assuntos
Humanos , Masculino , Feminino , Adolescente , Família/psicologia , Ideação Suicida , Relações Familiares
13.
Psicol. reflex. crit ; 27(1): 171-178, Jan.-Mar. 2014. tab
Artigo em Inglês | Index Psicologia - Periódicos | ID: psi-67996

RESUMO

The purpose of this study is to analyze how family systems influence adolescents' suicidal ideation. The participants were 534 adolescents (51.1% female and 48.9% male), with ages ranging from 14 to 18 years (M=16.18; SD=1.14). The instruments used were the following: Family Adaptability and Cohesion Evaluation Scale, Psychological Separation Inventory and Questionnaire of Suicidal Ideation. Results have shown that adolescents with higher suicidal ideation belong to the so-called unbalanced families (with low cohesion and flexibility) and have a conflictual dependence to their parents. On the other hand, adolescents that belong to balanced families revealed lower suicidal ideation. In conclusion, the family's cohesion and flexibility are protective factors against adolescents' suicidal ideation.(AU)


O objetivo deste estudo é analisar de que forma o funcionamento familiar influencia a ideação suicida na adolescência. Para tal, analisámos a coesão e adaptabilidade familiar, assim como a independência conflitual. Participaram neste estudo 534 adolescentes (51,1% raparigas e 48,9% rapazes), com idades compreendidas entre 14 e 18 anos (M=16,18; DP=1,14). Os instrumentos utilizados foram: Escala de Adaptabilidade e Coesão Familiar; Inventário Separação Psicológica e Questionário Ideação Suicida. Os resultados mostraram que os adolescentes com maior ideação suicida pertencem a famílias desequilibradas (coesão e adaptabilidade baixa) e têm uma forte dependência conflitual a ambos os pais. Os adolescentes pertencentes a famílias equilibradas revelaram menor ideação suicida. A coesão e a capacidade adaptativa da família são fatores protetores da ideação suicida.(AU)


Assuntos
Humanos , Masculino , Feminino , Adolescente , Ideação Suicida , Família/psicologia , Relações Familiares
14.
Rev Esc Enferm USP ; 48(6): 1111-8, 2014 Dec.
Artigo em Português | MEDLINE | ID: mdl-25626512

RESUMO

OBJECTIVE: To analyze the production of scientific knowledge about the use of patients' classification instruments in care and management practice in Brazil. METHOD: Integrative literature review with databases search in: Latin American and Caribbean Literature on Health Sciences (LILACS), Medical Literature Analysis and Retrieval System on-line (MEDLINE), Cumulative Index to Nursing and Allied Health Literature (CINAHL) and SCOPUS, between January 2002 through December 2013. RESULTS: 1,194 studies were found, 31 met the inclusion criteria. We observed a higher number of studies in the category care plans and workload (n=15), followed by the category evaluation of psychometric properties (n=14). CONCLUSION: Brazilian knowledge production has not yet investigated some purposes of using instruments for classifying patients in professional nursing practice. The identification of unexplored areas can guide future research on the topic.



Assuntos
Bibliometria , Processo de Enfermagem/estatística & dados numéricos , Brasil , Humanos , Avaliação em Enfermagem/estatística & dados numéricos , Psicometria , Reprodutibilidade dos Testes
15.
J Chromatogr A ; 1242: 75-83, 2012 Jun 15.
Artigo em Inglês | MEDLINE | ID: mdl-22560453

RESUMO

A comparison of electrospray ionization (ESI), atmospheric pressure chemical ionization (APCI) and atmospheric pressure photoionization (APPI) for the analysis of a wide range of lipids has been performed on standard mixtures and extracts of Leishmania donovani promastigotes resistant to Amphotericin B (AmB). Calibration model, precision, limits of detection and quantification (LOD and LOQ) were assessed for each source. APPI provided the highest signal, signal-to-noise (S/N), and sensitivity for non-polar and low-polarity lipids, while ESI and APCI gave better results for the most polar ones. The linear model was valid for all lipids, except for one class with APPI, six classes with ESI, and eleven classes with APCI. LODs ranged from 0.2 to 20 µg mL(-1) for ESI, from 0.1 to 10 µg mL(-1) for APCI, and from 0.02 to 9.5 µg mL(-1) for APPI. LOQs ranged from 0.2 to 61 µg mL(-1) for ESI, from 0.4 to 31 µg mL(-1) for APCI, and from 0.1 to 29 µg mL(-1) for APPI. Each source provided similar lipid composition and variations in a comparison of three different L. donovani samples: miltefosine-treated, miltefosine-resistant and treated miltefosine-resistant parasites. A treated miltefosine-resistant sample was finally analyzed with each ion source in order to verify that the same lipid molecular species are detected.


Assuntos
Leishmania donovani/química , Lipídeos/análise , Fotoquímica/métodos , Espectrometria de Massas por Ionização por Electrospray/métodos , Pressão Atmosférica , Resistência a Medicamentos , Leishmania donovani/efeitos dos fármacos , Leishmania donovani/metabolismo , Limite de Detecção , Lipídeos/química , Metaboloma/efeitos dos fármacos , Fosforilcolina/análogos & derivados , Fosforilcolina/farmacologia , Reprodutibilidade dos Testes , Razão Sinal-Ruído
16.
J Atheroscler Thromb ; 18(6): 494-503, 2011.
Artigo em Inglês | MEDLINE | ID: mdl-21325776

RESUMO

AIM: Dietary supplements in polyunsaturated fatty acids (PUFA), particularly omega-3, are well known for their beneficial effects in preventing cardiovascular diseases (CVD). The aim of this study was to determine the role of PUFA on the modulation of apoptosis induced by hypochlorous acidoxidized LDL (HOCl-oxLDL) in U937 cells. METHODS: We tested the effect of monocyte cell line U937 supplementation with eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), arachidonic acid (ARA) or oleic acid (OA) on the modulation of HOCl-oxLDL-induced apoptosis. RESULTS: First, we showed the incorporation of fatty acids in the cellular membrane in U937 cells. Then, we showed that both EPA and ARA exerted a pro-apoptotic effect through the intrinsic mitochondrial apoptotic pathway including the dissipation of mitochondrial membrane potential followed by cardiolipin depletion, the downstream activation of caspase-3 and the increase in DNA fragmentation. The pro-apoptotic effect of EPA or ARA was completely blocked in U937/Bcl-2 cells. CONCLUSIONS: A new mechanism of dietary supplements in PUFA with likely consequences in apoptosis could be suggested through the mitochondrial pathway in monocytes.


Assuntos
Apoptose , Ácido Araquidônico/farmacologia , Ácidos Docosa-Hexaenoicos/farmacologia , Ácido Eicosapentaenoico/farmacologia , Lipoproteínas LDL/metabolismo , Mitocôndrias/efeitos dos fármacos , Monócitos/efeitos dos fármacos , Cardiolipinas/metabolismo , Caspase 3/metabolismo , Suplementos Nutricionais , Ativação Enzimática/efeitos dos fármacos , Ácidos Graxos/metabolismo , Humanos , Potencial da Membrana Mitocondrial/efeitos dos fármacos , Mitocôndrias/metabolismo , Monócitos/citologia , Monócitos/metabolismo , Células U937
17.
Rev. bras. ter. intensiva ; 19(1): 5-13, jan.-mar. 2007. ilus, graf, tab
Artigo em Português | LILACS | ID: lil-466763

RESUMO

JUSTIFICATIVA E OBJETIVOS: A dobutamina é um agente inotrópico com propriedade adrenérgica beta-1 predominante e freqüentemente usado para aumentar o fluxo sanguíneo em pacientes críticos. Dobutamina pode ter um papel no aumento da perfusão esplâncnica, desse modo protegendo esta área de lesão isquêmica. O objetivo deste estudo foi investigar os efeitos de baixas doses de dobutamina (5 mig/kg/min) sobre a oxigenação tecidual, resposta inflamatória e complicações pós-operatória em pacientes de alto risco. MÉTODO: Estudo prospectivo, aleatório, encoberto e placebo-controlado. Cem pacientes cirúrgicos admitidos em Unidade Semi-Intensiva foram avaliados e 82 pacientes foram incluídos, 42 pacientes no grupo controle (solução fisiológica) e 40 no grupo tratamento (5 mig/kg/min) de dobutamina por 24 horas). Os mesmos procedimentos terapêuticos foram usados nos dois grupos. A infusão de líquidos deveria ser realizada em caso de taquicardia ou hipotensão após a infusão de dobutamina, pela possibilidade de hipovolemia. RESULTADOS: O volume total de fluidos administrado foi significativamente maior no grupo dobutamina do que no grupo controle (7351 ± 2082 mL versus 6074 ± 2386 mL, respectivamente, p < 0,05). Saturação venosa central de oxigênio (SvcO2), lactato sérico e proteína C-reativa foram similares em ambos os grupos. Complicações ocorreram em 35 por cento e 50 por cento dos pacientes nos grupos dobutamina e controle, respectivamente (RR 0,70 IC 95 por cento 0,41 - 1,17; NS). CONCLUSÕES: Baixas doses de dobutamina e fluidos após trauma cirúrgico não tiveram efeitos na prevalência de complicações pós-operatória em pacientes cirúrgicos de alto risco.


BACKGROUND AND OBJECTIVES: Dobutamine is an inotropic agent with predominant beta1- adrenergic properties frequently used to increase blood flow in critically ill patients. Dobutamine may have a role in increasing splanchnic perfusion, thereby protecting this area from further injury. We investigated the effects of low doses dobutamine (5 mug/kg/min) on tissue oxygenation, inflammatory response and postoperative complications in high-risk surgical patients. METHODS: Prospective, randomized, blinded and placebo-controlled study. One hundred surgical patients admitted in a step-down unit were evaluated and 82 patients were enrolled, 42 in the control group (saline) and 40 in the treatment group (5 mug/kg/h) during 24 hours. Similar therapeutic goals were applied to both groups. Fluids were given whenever tachycardia or hypotension developed after study drug infusion. RESULTS: The total volume of fluids given was significantly higher in treatment than in control group (7351 ± 2082 mL versus 6074 ± 2386 mL, respectively, p < 0.05). Central venous oxygen saturation (ScvO2), serum lactate and C-reactive protein were similar in both groups. Complications occurred in 35 percent and 50 percent of the patients in the treatment and control groups, respectively (RR 0, 70 IC 95 percent 0.41 - 1.17; NS). CONCLUSIONS: Low-doses dobutamine and fluids after surgical trauma has no effects on the prevalence of postoperative complications in high-risk surgical patients.


Assuntos
Humanos , Masculino , Feminino , Dobutamina/administração & dosagem , Pacientes Internados , Unidades de Terapia Intensiva
18.
Rev Bras Ter Intensiva ; 19(1): 5-13, 2007 Mar.
Artigo em Português | MEDLINE | ID: mdl-25310654

RESUMO

BACKGROUND AND OBJECTIVES: Dobutamine is an inotropic agent with predominant beta1- adrenergic properties frequently used to increase blood flow in critically ill patients. Dobutamine may have a role in increasing splanchnic perfusion, thereby protecting this area from further injury. We investigated the effects of low doses dobutamine (5 mug/kg/min) on tissue oxygenation, inflammatory response and postoperative complications in high-risk surgical patients. METHODS: Prospective, randomized, blinded and placebo-controlled study. One hundred surgical patients admitted in a step-down unit were evaluated and 82 patients were enrolled, 42 in the control group (saline) and 40 in the treatment group (5 mug/kg/h) during 24 hours. Similar therapeutic goals were applied to both groups. Fluids were given whenever tachycardia or hypotension developed after study drug infusion. RESULTS: The total volume of fluids given was significantly higher in treatment than in control group (7351 ± 2082 mL versus 6074 ± 2386 mL, respectively, p < 0.05). Central venous oxygen saturation (ScvO2), serum lactate and C-reactive protein were similar in both groups. Complications occurred in 35% and 50% of the patients in the treatment and control groups, respectively (RR 0, 70 IC 95% 0.41 - 1.17; NS). CONCLUSIONS: Low-doses dobutamine and fluids after surgical trauma has no effects on the prevalence of postoperative complications in high-risk surgical patients.

19.
Arch. Hosp. Vargas ; 44(1/2): 18-28, ene.-jun. 2002. tab, graf
Artigo em Espanhol | LILACS | ID: lil-365567

RESUMO

Las exacerbaciones agudas de la Enfermedad Broncopulmonar Obstructiva Crónica (EBPOC) son frecuentes y muchas veces difíciles de tratar. Determinar el efecto broncodilatador del sulfato de magnesio en el tratamiento de pacientes con exacerbaciones del EBPOC. Estudio randomizado, controlado, dobre ciego. 10 pacientes con diagnóstico de EBPOC que acudieron a Emergencia del Hospital Domingo Luciani entre julio y septiembre de 1999 con exacerbación de su enfermedad, recibieron una infusión intravenosa de 20 meq de Magnesio o placebo durante 20 min luego de una primera dosis de salbutamol nebulizado. El flujo espiratorio pico (FEP), escore de disnea y signos vitales fueron evaluados 40 min después del inicio de la infusión. El FEP al minuto 60 fue mayor en el grupo magnesio que en grupo placebo (250 ± 84,853 vs 147 ± 42,71; p=0,041); la frecuencia respiratoria (34 ± 5,657 vs 25,6 ± 5,177; p=0,00) y el escore de disnea al min 60 (rango 3 vs 8, p=0,0079), fueron menores en el grupo magnesio. Diferencias estadísticamente significativas. El sulfato de magnesio es eficaz en el tratamiento de las exacerbaciones del EBPOC, logrando un efecto broncodilatador mayor y más rápido que cuando se administran sólo Beta2 agonistas.


Assuntos
Humanos , Masculino , Feminino , Albuterol , Antagonistas Colinérgicos/administração & dosagem , Antagonistas Colinérgicos/análise , Broncodilatadores , Sulfato de Magnésio , Pneumopatias Obstrutivas/complicações , Pneumopatias Obstrutivas/terapia , Placebos , Medicina , Venezuela
20.
Arch. Hosp. Vargas ; 43(3/4): 208-218, jul.-dic. 2001. tab
Artigo em Espanhol | LILACS | ID: lil-347229

RESUMO

A comienzos de la epidemia del Síndrome de Inmunodeficiencia Adquirida (SIDA), el Pneumocytis carinii era la causa más común de infección respiratoria en estos pacientes. Realizar el diagnóstico etiológico de la infección respiratoria baja (IRB) en pacientes con infección por virus de inmunodeficiencia humana (VIH) usando aspiración transtraqueal (ATT) como método diagnóstico. Estudio epidemiológico clínico, analítico, descriptivo, prospectivo que evaluó las manifestaciones clínicas y hallazgos de laboratorio, gasometría y radiología en 15 pacientes que ingresaron al Hospital Dr. Domingo Luciani entre enero y marzo de 1998, con IRB e infección por VIH al ingreso y días 7,14 y 30 siguientes. Se realizó ATT como método diagnóstico etiológico de IRB, con examen directo, coloraciones especiales y cultivos para bacterias, tuberculosis y hongos a todas las muestras obtenidas. Ningún paciente presentó complicaciones por este procedimiento. En 9 pacientes se determinó la etiología infecciosa, con predominio bacteriano (Pseudomona aeruginosa, 2 Staphylococcus aureus, 2; Nocardia asteroides, 2; Streptococo beta del grupo A, 1; Escherichia coli, 1; Esterobacter arogenes, 1; y Pneumocystis carinii, 1. Nuestro estudio evidencia la frecuencia bacterianas en pacientes con infección por VIH e IRB


Assuntos
Humanos , Síndrome de Imunodeficiência Adquirida , HIV , Inalação , Pneumologia , Infecções Respiratórias , Medicina , Venezuela
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