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1.
J Med Case Rep ; 15(1): 567, 2021 Nov 25.
Artigo em Inglês | MEDLINE | ID: mdl-34823580

RESUMO

BACKGROUND: Escherichia coli has appeared as an important opportunistic pathogen responsible for nosocomial infections in patients with immunodeficiency, particularly in leukemia patients. New Delhi metallo-beta-lactamase is an enzyme originally found in Enterobacteriaceae. CASE PRESENTATION: In this study, 80 isolates of Escherichia coli and Klebsiella pneumoniae were collected over the course of 2 years from two medical centers in Tehran, Iran. Production of carbapenemase was detected in the isolates using modified Hodge test. New Delhi metallo-beta-lactamase-1 genes were detected by polymerase chain reaction amplification with specific primers. Two New Delhi metallo-beta-lactamase-1-producing Escherichia coli strains were isolated from two Iranian patients with leukemia. These two patients were 6 and 15 years old, one female and the other male, from two oncology centers in Iran. The isolates were resistant to carbapenems (imipenem, meropenem), and two isolates were positive for carbapenemase production by modified Hodge test. CONCLUSIONS: The emergence of New Delhi metallo-beta-lactamase-1-producing Escherichia coli is a threat for leukemia patients in oncology and hematology departments. We conclude that the incidence of multidrug resistant pathogens has increased among patients with leukemia and is life threatening.


Assuntos
Escherichia coli , Leucemia , Adolescente , Antibacterianos/farmacologia , Antibacterianos/uso terapêutico , Criança , Farmacorresistência Bacteriana Múltipla/genética , Escherichia coli/genética , Feminino , Humanos , Irã (Geográfico)/epidemiologia , Masculino , Testes de Sensibilidade Microbiana , beta-Lactamases/genética
2.
BMC Res Notes ; 12(1): 28, 2019 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-30646938

RESUMO

OBJECTIVE: The present study aimed to determine the prevalence of virulence factors and antimicrobial resistance profile of Pseudomonas aeruginosa strains isolated from Iranian burn patients. RESULTS: This cross-sectional study performed on 100 P. aeruginosa isolates which were recovered from burn wound specimens in 2014-2015. All presumptive isolates were identified by standard microbiologic tests. Antimicrobial susceptibility test was carried out by disk diffusion method. The presence of virulence genes was determined by PCR method. Antibiotic susceptibility results revealed that the isolates were mostly susceptible to amikacin (61%), ceftazidime (60%), and imipenem (55%). Moreover, 59% of the isolates were multi-drug resistance (MDR). The most prevalent MDR pattern was aminoglycosides-penicillins-fluoroquinolones-carbapenems (15%). The presence of exoT, exoY, exoS and exoU genes was detected in 100%, 100%, 59%, and 41% of the tested isolates, respectively. Results points out the pattern of MDR and genetic diversity of type III secretion system among P. aeruginosa strains isolated from the burn population. Overall, the association of MDR and the presence of the specific virulence genes can be a predictive marker for the persistence of these isolates in the hospitals and subsequently a worse clinical condition for the affected patients.


Assuntos
ADP Ribose Transferases/genética , Antibacterianos , Proteínas de Bactérias/genética , Toxinas Bacterianas/genética , Unidades de Queimados , Queimaduras/microbiologia , Farmacorresistência Bacteriana Múltipla/genética , Proteínas Ativadoras de GTPase/genética , Glucosiltransferases/genética , Pseudomonas aeruginosa/genética , Sistemas de Secreção Tipo III , Fatores de Virulência/genética , Estudos Transversais , Humanos , Irã (Geográfico) , Testes de Sensibilidade Microbiana , Pseudomonas aeruginosa/isolamento & purificação
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