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1.
Artigo em Russo | MEDLINE | ID: mdl-25051705

RESUMO

AIM: Conduction of a comparative proteomic mass-spectrometric (MS) analysis using a personal database of V. cholerae protein mass-spectra and genetic VNTR-typing of cholera causative agent strains. MATERIALS AND METHODS: V. cholerae O1 El Tor strains - 7, V. cholerae non O1/non O139 - 2. Protein profiling and VNTR-genotyping of strains was carried out on MALDI TOF-MS Autoflex (Bruker Daltonics) mass-spectrometer and SIS Cholera-strains-VNTR. RESULTS: The established community of a proteomic profile of epidemic cholera vibrio strains isolated in 2010 - 2012 in Moscow allowed to determine Indian origin of a toxigenic strain isolated in Taganrog in 2011. M/z proteins distinguishing V. cholerae O1 and non O1/non O139 strains were identified. Proteomic analysis confirms the results of VNTR-genotyping. CONCLUSION: Study and typing of V. cholerae members with determination of their origin and phylogenetic relationship is possible using a collection of V. cholerae mass-spectra.


Assuntos
Proteínas de Fímbrias/genética , Repetições Minissatélites , Filogenia , Proteômica , Vibrio cholerae/genética , Cólera/epidemiologia , Cólera/microbiologia , Meios de Cultura , Proteínas de Fímbrias/classificação , Expressão Gênica , Loci Gênicos , Genótipo , Humanos , Tipagem de Sequências Multilocus/métodos , Filogeografia , Reação em Cadeia da Polimerase , Federação Russa/epidemiologia , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz , Vibrio cholerae/classificação , Vibrio cholerae/isolamento & purificação
2.
Klin Lab Diagn ; (5): 42-6, 2013 May.
Artigo em Russo | MEDLINE | ID: mdl-24006644

RESUMO

The article deals with the results of testing of new diagnostic anti-lipase preparation and bacterial technique of detection of hemolytic strains of cholera vibrio with purpose to implement them in the laboratory practice and standardization of detection of epidemically dangerous strains of cholera vibrio.


Assuntos
Vibrio cholerae/patogenicidade , Técnicas Bacteriológicas , Hemólise , Lipase/metabolismo , Técnicas de Diagnóstico Molecular , Vibrio cholerae/classificação , Vibrio cholerae/enzimologia , Virulência
3.
Klin Lab Diagn ; (5): 59-61, 2013 May.
Artigo em Russo | MEDLINE | ID: mdl-24006648

RESUMO

The approbation of diagnostic preparations on the substrate of monoclonal antibodies developed in the institute was carried out during tactical specialized exercise with building up of units on the basis of mobile complex of specialized anti-epidemic brigades. It is established that diagnostic agglutinating and fluorescent monoclonal immunoglobulins by their sensitivity are equal to polyclonal commercial preparations and can be used at the stages of laboratory diagnostic of cholera both in conditions of stationary laboratory and mobile complex of specialized anti-epidemic brigades. The method of dot immunoanalysis on the substrate of monoclonal antibodies can, on a par with such common methods as immunofluorescence, slide-agglutination and polymerase chain reaction, be applied in complex of methods of express-diagnostic of cholera.


Assuntos
Cólera/diagnóstico , Immunoblotting/métodos , Cólera/epidemiologia , Humanos
4.
Artigo em Russo | MEDLINE | ID: mdl-24000598

RESUMO

AIM: Evaluation of quality indicators of constructed cholera antigen polymer diagnosticums by using a complex of specific anti-cholera sera. MATERIALS AND METHODS. Cell lysates of cholera vibrio strains Vibrio cholerae cholerae 1395, V. eltor Ogawa 2044, V. eltor Inaba 13020, V. cholerae O139 16064 were sensitins for experimental preparations. 3 sera from cholera patients, normal human sera, cholera O1 (Ogawa, Inaba) commercial horse, cholera O139 commercial rabbit and heterologic sera against shigella, salmonella, escherichia and yersinia as well as experimental cholera rabbit sera against O1 and O139 were used as control. RESULTS: The study established that diagnosticums based on V. cholerae cholerae 1395 and V. cholerae O139 16064 strain sensitins by quality indicators may be used in the future for construction of these diagnosticums. CONCLUSION: Antibody containing preparations--commercial horse O1 sera, rabbit experimental and commercial sera and MCA O139 demonstrating titers not lower than 1/5120-1/10240 may serve as a control of experimental diagnosticums in the absence of human sera from cholera patients.


Assuntos
Antígenos de Bactérias/química , Cólera/diagnóstico , Imunoensaio , Polímeros/química , Sorotipagem/normas , Vibrio cholerae/química , Animais , Antígenos de Bactérias/imunologia , Cólera/sangue , Cólera/microbiologia , Cavalos , Humanos , Soros Imunes/química , Soros Imunes/imunologia , Coelhos , Kit de Reagentes para Diagnóstico/normas , Sorotipagem/métodos , Vibrio cholerae/imunologia
5.
Vestn Oftalmol ; 129(6): 34-7, 2013.
Artigo em Russo | MEDLINE | ID: mdl-24624800

RESUMO

Among known glaucoma triggers, pseudoexfoliation syndrome (PXF) is most of all studied. Development of open-angle glaucoma (OAG) in some patients with PXF and certain somatic diseases requires further investigation. This study was aimed to evaluation of glaucoma risk in patients with PXF in relation to concurrent somatic pathology. A total of 185 patients aged from 48 to 87 diagnosed with OAG and, additionally, 75 patients of control group were assessed. Complex examination was performed and anamnestic data collected in all cases. A statistically significant relationship between OAG development and the presence of primary hypertension, ischemic heart disease, chronic cerebral circulatory insufficiency, and atherosclerosis in patients with PXF has been established.


Assuntos
Doenças Cardiovasculares/epidemiologia , Síndrome de Exfoliação/complicações , Pressão Intraocular , Medição de Risco/métodos , Idoso , Idoso de 80 Anos ou mais , Doenças Cardiovasculares/complicações , Progressão da Doença , Síndrome de Exfoliação/fisiopatologia , Feminino , Seguimentos , Humanos , Incidência , Masculino , Pessoa de Meia-Idade , Fatores de Risco , Federação Russa/epidemiologia
6.
Artigo em Russo | MEDLINE | ID: mdl-21604389

RESUMO

AIM: Determination of non-O1/non-O139 Vibrio cholerae toxin (CT) gene expression by using EIA, and biological effect of non-O1/non-O139 V. cholerae supernatant on cell cultures evaluation. MATERIALS AND METHODS: 39 V. cholerae strains from various serological groups were studied. Hemolytic activity of strains was determined by using Greig test, and cholera toxin production--in GM1-EIA and in continuous cell lines by registering cytotonic, cytotoxic and proteolitic effect. RESULTS: GM1-EIA method does not detect CT production in 29 museum strains of non-O1/non-O139 V. cholerae in vitro. CT was detected only in 1 non-O1/non-O139 V. cholerae strain supernatant with OD = 0.577 that is substantially lower than in O1 V. cholerae strains (OD = 2.176). In cell cultures non-O1/non-O139 V. cholerae supernatants diluted to 1:100 caused elongation only in single cells. CONCLUSION: Cytological model is a more sensitive technique to evaluate toxin producing abilities of non-O1/non-O139 V. cholerae strains and is appropriate for use.


Assuntos
Toxina da Cólera/biossíntese , Vibrio cholerae O1/imunologia , Vibrio cholerae não O1/imunologia , Animais , Células CHO , Técnicas de Cultura de Células , Forma Celular , Cólera/imunologia , Cólera/patologia , Toxina da Cólera/imunologia , Toxina da Cólera/metabolismo , Cricetinae , Cricetulus , Eritrócitos/metabolismo , Técnica Direta de Fluorescência para Anticorpo , Hemólise , Humanos , Receptores de Superfície Celular/imunologia , Receptores de Superfície Celular/metabolismo , Relação Estrutura-Atividade , Vibrio cholerae O1/química , Vibrio cholerae não O1/química
7.
Artigo em Russo | MEDLINE | ID: mdl-21381382

RESUMO

AIM: Comparative study of sensitivity and specificity of immunochromatographic (IC) assay kit and dot-immunoanalysis for assessment of feasibility of their use for laboratory diagnostics of cholera. MATERIALS AND METHODS: Experimental lots of IC assay kit and dot-immunoassay (DIA) for detection of Vibrio cholerae serogroup 01 serovars Ogava and Inaba were constructed on the basis of species-specific monoclonal antibodies (MCA) conjugated with colloid gold (IC) and peroxidase (DIA). Hybridoma-producer of MCAwas obtained and stored in liquid nitrogen in Rostov-on-Don Research Institute for Plague Control. It was deposited in specialized collection of cell cultures of vertebrates in Institute of Cytology (Saint Petersburg). RESULTS: Strong specificity of IC assay kit and DIA relative to cholera vibrios 01 and absence of crossreactivity with closely related and heterologous microorganisms were shown. Minimal quantity of vibrios, which could be detected using IC assay kit and DIA, was 107 and 105-106 microbial cells respectively. CONCLUSION: Performance of IC assay takes 5-15 min, DIA--1.5 hour, they allow to visually assess the reaction, do not require instrumentation and in perspective both methods could be used on defined stages of scheme for laboratory analysis of cholera.


Assuntos
Cólera/diagnóstico , Cromatografia/métodos , Immunoblotting/métodos , Vibrio cholerae O1/isolamento & purificação , Anticorpos Antibacterianos/imunologia , Anticorpos Monoclonais/imunologia , Especificidade de Anticorpos , Coloide de Ouro , Humanos , Peroxidase , Sensibilidade e Especificidade , Vibrio cholerae O1/imunologia
8.
Artigo em Russo | MEDLINE | ID: mdl-17523437

RESUMO

Study showed that El-Tor strains of V. cholerae isolated from different sources produce lipase for hemolysis after cultivation during 24 h on meat-peptone broth independently from their toxigenic and hemolytic abilities. Study of 3- and 4-hours broth cultures of vibrios revealed possibility to differentiate between hemolytic nontoxigenic strains and toxigenic nonhemolytic ones. Using antilipaze diagnostic kit it was possible to differentiate El-Tor vibrios from vibrios of classic biovar basing on lipase production 24 h after cultivation on meat-peptone broth that was evident in El-Tor vibrios but not in classic biovar strains.


Assuntos
Cólera/diagnóstico , Lipase/análise , Vibrio cholerae O1/enzimologia , Cólera/epidemiologia , Meios de Cultura , Diagnóstico Diferencial , Surtos de Doenças , Humanos , Lipase/biossíntese , Lipase/imunologia , Kit de Reagentes para Diagnóstico , Federação Russa , Sensibilidade e Especificidade , Fatores de Tempo , Uzbequistão/epidemiologia , Vibrio cholerae O1/classificação , Vibrio cholerae O1/crescimento & desenvolvimento
9.
Artigo em Russo | MEDLINE | ID: mdl-16532643

RESUMO

The possibility of using a heterogeneous, but structurally similar antigen--the commercial preparation of Pseudomonas sp. lipase (Sigma, USA)--for the development of polymer diagnosticum aimed at determination of lipase production in cholera vibrios was shown. The new diagnosticum (antilipase antibodies) on a polymer carrier was used in the serological volume agglomeration test for the detection of hemolytic atoxigenic V. eltor, obtained from environmental, objects, which produced lipase in 80% of cases. The differentiating capacity of the diagnosticum was confirmed on 120 V. eltor cultures isolated from environmental objects. The newly developed diagnosticum makes it possible to determine the lipase activity in cholera vibrios of different biovars and serovars.


Assuntos
Técnicas Bacteriológicas/métodos , Microbiologia Ambiental , Vibrio cholerae O1/isolamento & purificação , Animais , Anticorpos Antibacterianos , Hemólise , Lipase/imunologia , Lipase/metabolismo , Polímeros , Pseudomonas/enzimologia , Coelhos , Vibrio cholerae O1/imunologia , Vibrio cholerae O1/metabolismo
10.
Lik Sprava ; (8): 74-6, 2005 Dec.
Artigo em Russo | MEDLINE | ID: mdl-16498790

RESUMO

A new donor antidiphtheritic immunoglobulin produced in the Hematology and Transfusiology Institute of AMS of Ukraine and Donetsk transfusiology station is discussed in the article. The medication, antidiphtheritic horse serum had been used during the diphtheria epidemic, as a medication in Donetsk clinics. The medication meets requirements of State and International Standards and is registered in Ukraine.


Assuntos
Antitoxina Diftérica/uso terapêutico , Difteria/tratamento farmacológico , Soros Imunes/imunologia , Imunoglobulinas/uso terapêutico , Animais , Doadores de Sangue , Difteria/imunologia , Antitoxina Diftérica/administração & dosagem , Cavalos , Humanos , Soros Imunes/administração & dosagem , Imunoglobulinas/administração & dosagem , Injeções Intramusculares , Ucrânia
11.
Artigo em Russo | MEDLINE | ID: mdl-12886640

RESUMO

The action of nitrosoguanidine (NG) on the culture of V. cholerae O139 P-16064 resulted in the appearance of mutant 16064 NG6, not agglutinating with commercial diagnostic serum O139. Its incapacity of agglutination was due to the sorption of the specific serum with strains V. cholerae O22 and R-variant RCA-385, which caused the loss of antibodies to common determinants. Experiments with the sorption of immune sera made it possible to suggest that one of the determinants of LPS O139, phosphate-galactose, was absent in NG mutant.


Assuntos
Antígenos de Bactérias/imunologia , Vibrio cholerae O139/imunologia , Antígenos de Bactérias/genética , Epitopos/genética , Epitopos/imunologia , Mutação , Nitrosoguanidinas , Vibrio cholerae O139/genética
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