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1.
J Phys Act Health ; : 1-6, 2020 Jan 08.
Artigo em Inglês | MEDLINE | ID: mdl-31918408

RESUMO

BACKGROUND: Little research has examined gender differences in the association of seeing others exercise, in the neighborhood context, with physical activity, particularly for diverse racial/ethnic groups. The authors examined the association between frequency of seeing people walk and aerobic activity by gender among Latinos. METHODS: The authors used cross-sectional 2015 National Health Interview Survey data on Latino participants ≥18 years (n = 5147). Multinomial logistic regression models estimated the association between seeing people walk and level of aerobic physical activity. RESULTS: Men reporting seeing people walk every 2 to 3 days and every day were more likely to meet the aerobic activity recommendation (odds ratio [OR] 2.02; 95% confidence interval [CI], 1.05-3.89 and OR 1.96; 95% CI, 1.23-3.14, respectively). Among women, those seeing people walk every day and every 2 to 3 days were likely to engage in some aerobic activity (OR 1.88; 95% CI, 1.26-2.80 and OR 2.16; 95% CI, 1.23-3.18, respectively) and meet the recommendation (OR 1.73; 95% CI, 1.24-2.42 and OR 1.66; 95% CI, 1.03-2.67, respectively). Women seeing people walk about once a week were also likely to engage in some activity (OR 3.06; 95% CI, 1.59-5.89). CONCLUSIONS: Among Latino men and women, seeing people walk is associated with meeting the aerobic activity guideline. Results suggest that adoption of physical activity may in part be driven by neighborhood-level behavioral norms and by inference characteristics of the neighborhood that support walking.

2.
J Neurol ; 266(8): 1919-1926, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31069529

RESUMO

BACKGROUND: Neurological disorders are clinically heterogeneous group of disorders and are major causes of disability and death. Several of these disorders are caused due to genetic aberration. A precise and confirmatory diagnosis in the patients in a timely manner is essential for appropriate therapeutic and management strategies. Due to the complexity of the clinical presentations across various neurological disorders, arriving at an accurate diagnosis remains a challenge. METHODS: We sequenced 1012 unrelated patients from India with suspected neurological disorders, using TruSight One panel. Genetic variations were identified using the Strand NGS software and interpreted using the StrandOmics platform. RESULTS: We were able to detect mutations in 197 genes in 405 (40%) cases and 178 mutations were novel. The highest diagnostic rate was observed among patients with muscular dystrophy (64%) followed by leukodystrophy and ataxia (43%, each). In our cohort, 26% of the patients who received definitive diagnosis were primarily referred with complex neurological phenotypes with no suggestive diagnosis. In terms of mutations types, 62.8% were truncating and in addition, 13.4% were structural variants, which are also likely to cause loss of function. CONCLUSION: In our study, we observed an improved performance of multi-gene panel testing, with an overall diagnostic yield of 40%. Furthermore, we show that NGS (next-generation sequencing)-based testing is comprehensive and can detect all types of variants including structural variants. It can be considered as a single-platform genetic test for neurological disorders that can provide a swift and definitive diagnosis in a cost-effective manner.


Assuntos
Análise de Dados , Predisposição Genética para Doença/genética , Testes Genéticos/métodos , Sequenciamento de Nucleotídeos em Larga Escala/métodos , Doenças do Sistema Nervoso/genética , Criança , Pré-Escolar , Estudos de Coortes , Feminino , Predisposição Genética para Doença/epidemiologia , Humanos , Índia/epidemiologia , Masculino , Herança Multifatorial/genética , Mutação/genética , Doenças do Sistema Nervoso/diagnóstico , Doenças do Sistema Nervoso/epidemiologia
3.
Acad Emerg Med ; 26(3): 303-316, 2019 03.
Artigo em Inglês | MEDLINE | ID: mdl-30667132

RESUMO

OBJECTIVES: Organizations to promote career networking and mentorship among women are recommended as a best practice to support the recruitment and retention of women physicians; however, the impact of such organizations is unknown. Our primary objective is to describe the impact of a national woman-focused organization for academic emergency physicians on retention and advancement. METHODS: We conducted semistructured interviews of past and present organization leaders, as well as members at varying stages in their careers. Physicians with experience in qualitative methods conducted interviews and coded all transcripts using inductive content analysis techniques. Themes were reviewed and discussed to ensure consensus. RESULTS: We performed 17 interviews lasting 20 to 30 minutes each, resulting in 476 total minutes of transcript. Participants represented varying stages of career experience, ranging from 2 to 35 years since residency completion (median = 9.5 years). Median years of participation in the woman-focused organization was 10 years. Over half (53%) of participants were past presidents of the organization. The dominant themes encompassed facilitating academic advancement through scholarly productivity, leadership experiences, awards, and promotions; mentorship and sponsorship; peer support and collaborations; reduced professional isolation; and initiatives to address systemic gender inequities and challenges, including strategies to navigate bias, promote pay equity, and advocate for family-friendly workplace policies. DISCUSSION: Active participation in a woman-focused professional organization enhances members' career retention and advancement by creating opportunities and relationships that facilitate leadership, enabling scholarly work to advance equity and inclusion, and cultivating a sense of belonging. While challenges and barriers persist, the myriad benefits of a women-focused professional organization reported by members and leaders represent important steps toward greater equity for women and other underrepresented groups in academic medicine.


Assuntos
Medicina de Emergência/organização & administração , Liderança , Médicas/organização & administração , Sociedades Médicas/organização & administração , Mobilidade Ocupacional , Docentes de Medicina/organização & administração , Feminino , Humanos , Mentores , Pesquisa Qualitativa
5.
Am J Health Promot ; 32(7): 1576-1581, 2018 09.
Artigo em Inglês | MEDLINE | ID: mdl-29551075

RESUMO

PURPOSE: We examined differences in the association between alcohol use and sedentary behavior by gender among adults. DESIGN: Cross-sectional study that employs a complex, multistage stratified probability cluster sample design. SETTING: National Health and Nutrition Examination Survey (NHANES) 2007 to 2014. PARTICIPANTS: NHANES participants aged ≥20 years and identifying as Hispanic, white, or black (N = 18 441). MEASURES: Demographic characteristics, sedentary behavior, and alcohol consumption. Sedentary behavior was measured based on the number of minutes of sedentary activity per week. Alcohol consumption in the last year was measured based on responses to items assessing whether the participant consumes alcoholic beverages and the number of alcoholic beverages consumed on average. ANALYSIS: Multinomial logistic regression analysis was used to estimate associations of alcohol use with categories of sedentary behavior by gender. RESULTS: Compared to women who reported not consuming alcohol, women who were heavy drinkers were significantly more likely to engage in high sedentary activity (odds ratio [OR]: 1.47; 95% confidence interval [CI]: 1.16-1.87), relative to low levels of sedentary activity. Similarly, women who were binge drinkers were more likely to engage in moderate levels of sedentary activity (OR: 1.39; 95% CI: 1.05-1.85). There were no significant associations between alcohol use and sedentary activity among men. CONCLUSION: Findings suggest that alcohol consumption should be considered in health promotion efforts targeting sedentary behavior among women.


Assuntos
Consumo de Bebidas Alcoólicas , Comportamento Sedentário , Estudos Transversais , Feminino , Humanos , Modelos Logísticos , Masculino , Pessoa de Meia-Idade , Inquéritos Nutricionais , Fatores Sexuais , Transtornos Relacionados ao Uso de Substâncias
6.
J Immigr Minor Health ; 20(2): 327-333, 2018 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-28382427

RESUMO

Our objective was to examine refugees' acute care use early in resettlement. We conducted a retrospective cohort study of acute care use, emergency room and hospital visits, by adult refugees arriving in Southern Connecticut between 2/1/2013 and 2/1/2015. We examined associations between any acute care use and collected demographic as well as health characteristics. Of the 248 refugees in our sample, 57% had a medical evaluation within 30 days of arrival. 102 (41%) had at least one acute care visit within 8 months of arrival. Male sex (OR 1.90, 95% CI 1.09-3.30) and prior history of hypertension (OR 2.87, 95% CI 1.06-7.33) were associated with greater likelihood of an acute care visit within 8 months of arrival, while having a medical evaluation within 30 days of arrival was associated with lower likelihood (OR 0.56, 95% CI 0.32-0.98). Designing systems to ensure timely evaluations of newly arrived refugees may reduce frequent acute care utilization.


Assuntos
Nível de Saúde , Saúde Mental/etnologia , Aceitação pelo Paciente de Cuidados de Saúde/etnologia , Refugiados/estatística & dados numéricos , Adulto , Fatores Etários , Connecticut/epidemiologia , Feminino , Humanos , Masculino , Estudos Retrospectivos , Fatores Sexuais , Fatores Socioeconômicos
7.
Cancer Med ; 6(5): 883-901, 2017 May.
Artigo em Inglês | MEDLINE | ID: mdl-28371134

RESUMO

Comprehensive genetic profiling of tumors using next-generation sequencing (NGS) is gaining acceptance for guiding treatment decisions in cancer care. We designed a cancer profiling test combining both deep sequencing and immunohistochemistry (IHC) of relevant cancer targets to aid therapy choices in both standard-of-care (SOC) and advanced-stage treatments for solid tumors. The SOC report is provided in a short turnaround time for four tumors, namely lung, breast, colon, and melanoma, followed by an investigational report. For other tumor types, an investigational report is provided. The NGS assay reports single-nucleotide variants (SNVs), copy number variations (CNVs), and translocations in 152 cancer-related genes. The tissue-specific IHC tests include routine and less common markers associated with drugs used in SOC settings. We describe the standardization, validation, and clinical utility of the StrandAdvantage test (SA test) using more than 250 solid tumor formalin-fixed paraffin-embedded (FFPE) samples and control cell line samples. The NGS test showed high reproducibility and accuracy of >99%. The test provided relevant clinical information for SOC treatment as well as more information related to investigational options and clinical trials for >95% of advanced-stage patients. In conclusion, the SA test comprising a robust and accurate NGS assay combined with clinically relevant IHC tests can detect somatic changes of clinical significance for strategic cancer management in all the stages.


Assuntos
DNA de Neoplasias/genética , DNA de Neoplasias/metabolismo , Sequenciamento de Nucleotídeos em Larga Escala/métodos , Imuno-Histoquímica/métodos , Neoplasias/terapia , Análise de Sequência de DNA/métodos , Linhagem Celular Tumoral , Variações do Número de Cópias de DNA , Estudos de Associação Genética , Predisposição Genética para Doença , Humanos , Neoplasias/genética , Neoplasias/metabolismo , Polimorfismo de Nucleotídeo Único , Reprodutibilidade dos Testes , Padrão de Cuidado , Translocação Genética
8.
BMC Health Serv Res ; 17(1): 32, 2017 01 13.
Artigo em Inglês | MEDLINE | ID: mdl-28086870

RESUMO

BACKGROUND: Rates of noncommunicable diseases (NCDs) such as type 2 diabetes are escalating in low and middle-income countries such as Brazil. Scalable primary care-based interventions are needed to improve self-management and clinical outcomes of adults with diabetes. This pilot study examines the feasibility, acceptability, and outcomes of training community health agents (CHAs) in Motivational Interviewing (MI)-based counseling for patients with poorly controlled diabetes in a primary care center in São Paulo, Brazil. METHODS: Nineteen salaried CHAs participated in 32 h of training in MI and behavioral action planning. With support from booster training sessions, they used these skills in their regular monthly home visits over a 6 month period with 57 diabetes patients with baseline HbA1cs > 7.0%. The primary outcome was patients' reports of the quality of diabetes care as measured by the Portuguese version of the Patient Assessment of Chronic Illness Care (PACIC) scale. Secondary outcomes included changes in patients' reported diabetes self-management behaviors and in A1c, blood pressure, cholesterol and triglycerides. We also examined CHAs' fidelity to and experiences with the intervention. RESULTS: Patients reported improvements over the 6 month period in quality of diabetes care received (PACIC score improved 33 (+/-19) to 68 (+/-21) (p < .001)). They reported increases in physical activity (p = .001), consumption of fruits and vegetables (p < .001) and medication adherence (p = .002), but no decreases in consumption of high-fat foods (p = .402) or sweets (p = .436). Participants had mean 6-month A1c levels 0.34% points lower than at baseline (p = .08) and improved mean LDL (-16.1 mg/dL, p = .005) and triglyceride levels (-38.725 mg/dL, p = .002). Of the 16 CHAs observed in fidelity assessments, 13 were categorized as medium- or high-performing on MI skills, while 3 were low-performing. CHAs expressed enthusiasm about learning new skills, and many described a shift from advice-giving to encouraging patients to define their own goals. CONCLUSION: In resource-scarce settings, it is essential to fully utilize existing primary care resources to stem the epidemic of diabetes and other NCDs. Our pilot results support the potential of training CHAs to incorporate effective diabetes self-management support into their routine patient encounters. TRIAL REGISTRATION: NCT02994095 12/14/2016 Registered retrospectively.


Assuntos
Diabetes Mellitus Tipo 2/tratamento farmacológico , Entrevista Motivacional/métodos , Adulto , Pressão Sanguínea , Brasil , Doença Crônica , Serviços de Saúde Comunitária/métodos , Feminino , Promoção da Saúde , Humanos , Masculino , Adesão à Medicação , Pessoa de Meia-Idade , Projetos Piloto , Atenção Primária à Saúde/métodos , Autocuidado/métodos
9.
Acad Emerg Med ; 24(4): 484-493, 2017 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-27976457

RESUMO

OBJECTIVES: There are many barriers impeding the conduct of high-quality emergency care research, particularly in low- and middle-income countries. Several of these barriers were originally outlined in 2013 as part of the Academic Emergency Medicine Global Health and Emergency Care Consensus Conference. This paper seeks to establish a broader consensus on the barriers to emergency care research globally and proposes a comprehensive array of new recommendations to overcome these barriers. METHODS: An electronic survey was conducted of a purposive sample of global emergency medicine research experts from around the world to describe the major challenges and solutions to conducting emergency care research in low-resource settings and rank them by importance. The Global Emergency Medicine Think Tank Clinical Research Working Group at the Society for Academic Emergency Medicine 2016 Annual Meeting utilized a modified Delphi technique for consensus-based decision making to categorize and expand upon these barriers and develop a comprehensive array of proposed solutions. RESULTS: The working group identified four broad categories of barriers to conducting emergency care research globally, including 1) the limited availability of research personnel, particularly those with prior research training; 2) logistic barriers and lack of standardization of data collection; 3) ethical barriers to conducting research in resource-limited settings, particularly when no local institutional review board is available; and 4) the relative dearth of funding for global emergency care research. Proposed solutions included building a diverse and interdisciplinary research team structured to promote mentorship of junior researchers, utilizing local research assistants or technologic tools such as telemedicine for language translation, making use of new tools such as mobile health (mHealth) to standardize and streamline data collection, identifying alternatives to local institutional review board approval and the use of community consent when appropriate, and increased advocacy for global emergency care research funding. CONCLUSIONS: Significant barriers to the conduct of high-quality global emergency care research persist, and innovative strategies need to be adopted to promote and grow the field of global emergency care research. This paper provides a global consensus on the most important barriers identified, as well as recommendations for cost-effective strategies for overcoming these barriers with the overall goal of promoting high-quality research and improving emergency care worldwide.


Assuntos
Coleta de Dados/normas , Serviços Médicos de Emergência , Saúde Global/economia , Pesquisa sobre Serviços de Saúde , Técnica Delfos , Medicina de Emergência , Pesquisa sobre Serviços de Saúde/economia , Pesquisa sobre Serviços de Saúde/ética , Humanos , Inquéritos e Questionários , Recursos Humanos
10.
Am J Public Health ; 106(4): 662-3, 2016 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-26890186

RESUMO

We sought to evaluate the relationship between state-level implementation of the Patient Protection and Affordable Care Act (ACA) and resettlement patterns among refugees. We linked federal refugee resettlement data to ACA expansion data and found that refugee resettlement rates are not significantly different according to state-level insurance expansion or cost. Forty percent of refugees have resettled to states without Medicaid expansion. The wide state-level variability in implementation of the ACA should be considered by federal agencies seeking to optimize access to health insurance coverage among refugees who have resettled to the United States.


Assuntos
Trocas de Seguro de Saúde/organização & administração , Implementação de Plano de Saúde/legislação & jurisprudência , Medicaid/legislação & jurisprudência , Patient Protection and Affordable Care Act , Refugiados/estatística & dados numéricos , Governo Estadual , Censos , Acesso aos Serviços de Saúde , Humanos , Medicaid/economia , Estudos Retrospectivos , Estados Unidos
11.
Afr J Emerg Med ; 6(2): 70-79, 2016 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30456070

RESUMO

Introduction: Sub-Saharan Africa (SSA) has a need for blood and blood products that exceeds current availability. This unmet demand is evidenced by persistently high mortality rates associated with potentially reversible conditions such as haemorrhage and anaemia. This study reviews current literature on blood donation in SSA to identify common local motivators and deterrents to blood donation and highlights specific interventions that have successfully increased the number of blood donors in this region. Methods: The authors searched the health literature to identify original studies conducted in SSA that addressed blood donation motivators and deterrents. Data were then extracted from each study to determine how often each study identified specific motivators and deterrents. Results: Pro-social motivation, such as altruism and the desire to have a positive effect on the community, is the most frequently noted reason for people in SSA countries to donate blood. Negative attitudes and misconceptions towards blood donation, in addition to perceived negative health effects caused by donation, are the two most frequently mentioned reasons that dissuade individuals from donating blood. Conclusion: Due to paucity of research on this topic, our understanding of blood donor behaviour in SSA is limited. Local traditions and cultures intimately shape individuals' proclivity towards the donation process. In order to change the attitudes and behaviours of many potential donors in SSA it is important to address the deterrents to blood donation, as many represent misconceptions or culture-specific beliefs that may be the ultimate driving force dictating donor behaviour.

12.
J Biol Chem ; 289(46): 31818-26, 2014 Nov 14.
Artigo em Inglês | MEDLINE | ID: mdl-25258312

RESUMO

The mammalian target of rapamycin (mTOR) pathway regulates stem cell regeneration and differentiation in response to growth factors, nutrients, cellular energetics, and various extrinsic stressors. Inhibition of mTOR activity has been shown to enhance the regenerative potential of pluripotent stem cells. DEPTOR is the only known endogenous inhibitor of all known cellular mTOR functions. We show that DEPTOR plays a key role in maintaining stem cell pluripotency by limiting mTOR activity in undifferentiated embryonic stem cells (ESCs). DEPTOR levels dramatically decrease with differentiation of mouse ESCs, and knockdown of DEPTOR is sufficient to promote ESC differentiation. A strong decrease in DEPTOR expression is also observed during human ESCs differentiation. Furthermore, reduction in DEPTOR level during differentiation is accompanied by a corresponding increase in mTOR complex 1 activity in mouse ESCs. Our data provide evidence that DEPTOR is a novel stemness factor that promotes pluripotency and self-renewal in ESCs by inhibiting mTOR signaling.


Assuntos
Células-Tronco Embrionárias/citologia , Peptídeos e Proteínas de Sinalização Intracelular/metabolismo , Células-Tronco Pluripotentes/citologia , Proteínas Adaptadoras de Transdução de Sinal/genética , Animais , Diferenciação Celular , Linhagem Celular , Células HEK293 , Humanos , Peptídeos e Proteínas de Sinalização Intracelular/genética , Camundongos , Neurogênese , Fator 3 de Transcrição de Octâmero/metabolismo , Regiões Promotoras Genéticas , Transdução de Sinais , Células-Tronco/citologia , Serina-Treonina Quinases TOR/metabolismo , Proteína 2 do Complexo Esclerose Tuberosa , Proteínas Supressoras de Tumor/genética
13.
Confl Health ; 8: 6, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-24829613

RESUMO

BACKGROUND: Human security shifts traditional concepts of security from interstate conflict and the absence of war to the security of the individual. Broad definitions of human security include livelihoods and food security, health, psychosocial well-being, enjoyment of civil and political rights and freedom from oppression, and personal safety, in addition to absence of conflict. METHODS: In March 2010, we undertook a population-based health and livelihood study of female refugees from conflict-affected Central African Republic living in Djohong District, Cameroon and their female counterparts within the Cameroonian host community. Embedded within the survey instrument were indicators of human security derived from the Leaning-Arie model that defined three domains of psychosocial stability suggesting individuals and communities are most stable when their core attachments to home, community and the future are intact. RESULTS: While the female refugee human security outcomes describe a population successfully assimilated and thriving in their new environments based on these three domains, the ability of human security indicators to predict the presence or absence of lifetime and six-month sexual violence was inadequate. Using receiver operating characteristic (ROC) analysis, the study demonstrates that common human security indicators do not uncover either lifetime or recent prevalence of sexual violence. CONCLUSIONS: These data suggest that current gender-blind approaches of describing human security are missing serious threats to the safety of one half of the population and that efforts to develop robust human security indicators should include those that specifically measure violence against women.

14.
Acad Emerg Med ; 20(12): 1272-7, 2013 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-24341582

RESUMO

As policy-makers increasingly recognize emergency care to be a global health priority, the need for high-quality clinical and translational research in this area continues to grow. As part of the proceedings of the 2013 Academic Emergency Medicine consensus conference, this article discusses the importance of: 1) including clinical and translational research in the initial emergency care development plan, 2) defining the burden of acute disease and the barriers to conducting research in resource-limited settings, 3) assessing the appropriateness and effectiveness of local and global acute care guidelines within the local context, 4) studying the local research infrastructure needs to understand the best methods to build a sustainable research infrastructure, and 5) studying the long-term effects of clinical research programs on health care systems.


Assuntos
Medicina de Emergência , Saúde Global , Pesquisa sobre Serviços de Saúde , Pesquisa , Pesquisa Médica Translacional , Conferências de Consenso como Assunto , Prioridades em Saúde , Humanos
15.
Glob Public Health ; 7(9): 974-94, 2012.
Artigo em Inglês | MEDLINE | ID: mdl-22621466

RESUMO

The following is a population-based survey of the Central African Republic (CAR) female refugee population displaced to rural Djohong District of Eastern Cameroon and associated female Cameroonian host population to characterise the prevalence and circumstances of sexual violence. A population-based, multistage, random cluster survey of 600 female heads of household was conducted during March 2010. Women heads of household were asked about demographics, household economy and assets, level of education and sexual violence experienced by the respondent only. The respondents were asked to describe the circumstances of their recent assault. The lifetime prevalence of sexual violence among Djohong district female heads of household is 35.2% (95% CI 28.7-42.2). Among heads of household who reported a lifetime incident of sexual violence, 64.0% (95% CI 54.3-72.5) suffered sexual violence perpetrated by their husband or partner. Among the host population, 3.9% (95% CI 1.4-10.5) reported sexual violence by armed groups compared to 39.0% (95% CI 25.6-54.2) of female refugee heads of household. Women who knew how to add and subtract were less likely to report sexual violence during their lifetime (OR 0.16, 95% CI 0.08-0.34). Sexual violence is common among refugees and host population in Eastern Cameroon. Most often, perpetrators are partners/husbands or armed groups.


Assuntos
Violência Doméstica/estatística & dados numéricos , Refugiados/estatística & dados numéricos , Delitos Sexuais/estatística & dados numéricos , Adulto , Camarões/epidemiologia , Análise por Conglomerados , Violência Doméstica/psicologia , Escolaridade , Feminino , Humanos , Masculino , Estado Civil , Prevalência , Refugiados/psicologia , Delitos Sexuais/psicologia , Parceiros Sexuais
16.
J Biol Chem ; 287(25): 21164-75, 2012 Jun 15.
Artigo em Inglês | MEDLINE | ID: mdl-22544753

RESUMO

The mammalian target of rapamycin (mTOR) is an atypical serine/threonine kinase that responds to extracellular environment to regulate a number of cellular processes. These include cell growth, proliferation, and differentiation. Although both kinase-dependent and -independent functions of mTOR are known to be critical modulators of muscle cell differentiation and regeneration, the signaling mechanisms regulating mTOR activity during differentiation are still unclear. In this study we identify a novel mTOR interacting protein, the ubiquitin-specific protease USP9X, which acts as a negative regulator of mTOR activity and muscle differentiation. USP9X can co-immunoprecipitate mTOR with both Raptor and Rictor, components of mTOR complexes 1 and 2 (mTORC1 and -2), respectively, suggesting that it is present in both mTOR complexes. Knockdown of USP9X leads to increased mTORC1 activity in response to growth factor stimulation. Interestingly, upon initiation of differentiation of C2C12 mouse skeletal myoblasts, knockdown of USP9X increases mTORC2 activity. This increase in mTORC2 activity is accompanied by accelerated differentiation of myoblasts into myotubes. Taken together, our data describe the identification of the deubiquitinase USP9X as a novel mTORC1 and -2 binding partner that negatively regulates mTOR activity and skeletal muscle differentiation.


Assuntos
Diferenciação Celular/fisiologia , Endopeptidases/metabolismo , Desenvolvimento Muscular/fisiologia , Proteínas Musculares/metabolismo , Mioblastos Esqueléticos/metabolismo , Serina-Treonina Quinases TOR/metabolismo , Ubiquitina Tiolesterase/metabolismo , Proteínas Adaptadoras de Transdução de Sinal/genética , Proteínas Adaptadoras de Transdução de Sinal/metabolismo , Animais , Proteínas de Transporte/genética , Proteínas de Transporte/metabolismo , Endopeptidases/genética , Células HEK293 , Humanos , Alvo Mecanístico do Complexo 1 de Rapamicina , Camundongos , Complexos Multiproteicos , Proteínas Musculares/genética , Mioblastos Esqueléticos/citologia , Proteínas/genética , Proteínas/metabolismo , Proteína Companheira de mTOR Insensível à Rapamicina , Proteína Regulatória Associada a mTOR , Serina-Treonina Quinases TOR/genética , Transativadores/genética , Transativadores/metabolismo , Fatores de Transcrição/genética , Fatores de Transcrição/metabolismo , Ubiquitina Tiolesterase/genética
17.
Cell Cycle ; 9(24): 4908-21, 2010 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-21150319

RESUMO

Mammalian c-Myc is a member of a small family of three closely related transcription factors. The Myc family of proto-oncogenes are among the most potent activators of tumorigenesis, and are frequently overexpressed in diverse cancers. c-Myc has an unusually broad array of regulatory functions, which include, in addition to roles in the cell cycle and apoptosis, effects on a variety of metabolic functions, cell differentiation, senescence, and stem cell maintenance. A significant number of c-Myc interacting proteins have already been defined, but it is widely believed that the c-Myc interactome is vastly larger than currently documented. In addition to interactions with components of the transcription machinery, transcription independent nuclear interactions with the DNA replication and RNA processing pathways have been reported. Cytoplasmic roles of c-Myc have also been recently substantiated. Recent advances in proteomics have opened new possibilities for the isolation of protein complexes under native conditions and confidently identifying the components using ultrasensitive, high mass accuracy and high resolution mass spectrometry techniques. In this communication we report a new tandem affinity purification (TAP) c-Myc interaction screen that employed new cell lines with near-physiological levels of c-Myc expression with multi-dimensional protein identification techniques (MudPIT) for the detection and quantification of proteins. Both label-free and the recently developed stable isotope labeling with amino acids in cell culture (SILAC) methodologies were used. Combined data from multiple biological replicates provided a dataset of 418 non-redundant proteins, 389 of which are putative novel interactors. This new information should significantly advance our understanding of this interesting and important master regulator.


Assuntos
Cromatografia de Afinidade/métodos , Proteoma/análise , Proteômica/métodos , Proteínas Proto-Oncogênicas c-myc/isolamento & purificação , Animais , Linhagem Celular , Perfilação da Expressão Gênica , Humanos , Camundongos , Proteínas Proto-Oncogênicas c-myc/genética , Proteínas Proto-Oncogênicas c-myc/metabolismo , Proteínas Recombinantes de Fusão/genética , Proteínas Recombinantes de Fusão/isolamento & purificação , Proteínas Recombinantes de Fusão/metabolismo , Espectrometria de Massas em Tandem/métodos
18.
J Vector Borne Dis ; 47(1): 39-44, 2010 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-20231772

RESUMO

BACKGROUND & OBJECTIVES: Resistance amongst cockroaches has been reported to most of the spray insecticides apart from the problem of food contamination and inconvenience. Gel baits which can be selectively applied have been found effective in control of cockroaches in laboratory studies but very few field studies are available. This trial was planned to evaluate the efficacy of fipronil (0.01%) and imidacloprid (2.15%) gels over synthetic pyrethroid (0.02% deltamethrin + 0.13% allethrin) and propoxur (2%) aerosols in control of cockroaches in the field. METHODS: Survey was done to find out pre-treatment density in catering establishments and houses by visual count and sticky trap methods. A total of 10 catering establishments and 10 houses having high cockroach infestation were selected by sampling (two catering establishments and houses for each insecticidal treatment and two for control). Propoxur and synthetic pyrethroid aerosols were used for spraying the infested sites once only. Single application of fipronil and imidacloprid gels was used as crack and crevice treatment. Visual count method gave better indications of cockroach infestation as compared to sticky trap method, hence, the same was followed for post-treatment evaluation every week up to 12 weeks. RESULTS: Synthetic pyrethroid could not bring about the desired reduction in cockroach infestation in the present study. Single application of fipronil gel was able to reduce cockroach infestation up to 96.8% at the end of 12 weeks whereas imidacloprid application resulted in 90.9% reduction and propoxur resulted in 77.5%. However, propoxur was more effective in reducing the cockroach density by first week in comparison to imidacloprid and fipronil gels but its efficacy started declining after 8th week. Difference was found statistically significant by Kruskal-Wallis H-test. CONCLUSION: The study reports the efficacy of propoxur aerosol, imidacloprid gel and fipronil gel baits for control of cockroaches.


Assuntos
Baratas/efeitos dos fármacos , Controle de Insetos/métodos , Inseticidas/farmacologia , Aerossóis/farmacologia , Animais , Géis/farmacologia , Humanos , Densidade Demográfica
19.
Emerg Med Clin North Am ; 27(4): 555-67, vii, 2009 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-19932391

RESUMO

Many professional societies publish clinical practice guidelines that pertain to the management of patients with specific diagnoses. This article explores clinical practice guidelines affecting emergency physicians, such as those published by the American College of Emergency Physicians and other medical specialty organizations, such as the American Heart Association. Examples of such guidelines include algorithms, such as those published in Advanced Cardiac Life Support, clinical decision rules, such as the Ottawa ankle rules, and pathways that describe the treatment of critical diseases such as acute myocardial infarction. This article discusses the relevance of these guidelines, algorithms, and protocols to the practicing emergency medicine specialist.


Assuntos
Medicina de Emergência , Guias de Prática Clínica como Assunto , Algoritmos , Protocolos Clínicos , Procedimentos Clínicos , Medicina de Emergência Baseada em Evidências , Humanos , Terminologia como Assunto , Estados Unidos
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