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1.
Animals (Basel) ; 12(12)2022 Jun 16.
Artigo em Inglês | MEDLINE | ID: mdl-35739890

RESUMO

Eimeria (E.) maxima invades the midgut of chickens and destroys the intestinal mucosa, impacting nutrient digestibility and absorption. Heat stress (HS) commonly affects the broiler chicken and contributes to inflammation and oxidative stress. We examined the independent and combined effects of HS and E. maxima infection on apparent amino acid ileal digestibility (AID) and mRNA expression of amino acid transporters in broiler chickens (Ross 708). There were four treatment groups: thermoneutral-control (TNc) and infected (TNi), heat-stress control (HSc) and infected (HSi), six replicates of 10 birds/treatment. Ileal content and tissue were sampled at 6 d post infection to determine AID and transporters expression. Surprisingly, the HSi chickens exposed to two critical stressors exhibited normal AID. Only the TNi group displayed reduction in AID. Using TNc as control, the HSc group showed upregulated CAT1, LAT4, TAT1, SNAT1, and SNAT7. The HSi group showed upregulated CAT1 and LAT1, and downregulated b0,+AT, rBAT, SNAT1, and SNAT2. The TNi group showed upregulated CAT1, LAT1, and SNAT1 and downregulated B0AT1, b0,+AT, rBAT, LAT4, and TAT1. The expression of all enterocytic-apical and about half of the basolateral transporters was higher in the HSi group than in the TNi group, indicating that HS can putatively alleviate the E. maxima adverse effect on ileal digestion and absorption.

2.
PLoS One ; 17(6): e0269131, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35657942

RESUMO

Eimeria (E.) maxima is one of the most pathogenic Eimeria spp persistently invading the middle jejunum and ileum, damaging the intestinal mucosa of chickens. Heat stress (HS) is a common stressor and equally contributes to inflammation and oxidative stress. We investigated the effect of E. maxima infection and HS on ileal digestibility, mRNA expression of nutrient transporters, and ileal tissue morphology in broiler chickens. There were four treatment groups: thermoneutral control (TNc), thermoneutral infected (TNi), heat stress control (HSc), and heat stress infected (HSi), 6 replicates each of 10 birds per treatment. Chickens were fed a diet containing 0.2% TiO2. At 6-day-post infection, ileal content and tissue were collected to quantify ileal digestibility of crude protein and fat, mRNA levels of nutrient transporters and histopathology. Growth and feed intake were reduced in all treatment groups, compared with the TNc. Contrary to expectation, the combination of two major stressors (E. maxima and HS) in the TNi group exhibited almost normal digestibility while only the TNi birds expressed severe digestibility depression, compared with the TNc group. The TNi group showed the lowest mRNA expression of the transporters: SGLT1, GLUT2-5-8-10-12, FABP1-2-6, and PEPT1 compared with the other treatment groups. The expression of the absorptive enterocytes' gene markers (ACSL5, IAP, and SGLT1) supported by the ileal tissue morphology indicated that the TNi group had the highest enterocytic destruction. The expression of oxidative genes (iNOS and CYBB) dramatically increased only in the TNi group compared with the other treatment groups. Our results showed that exposing broiler chickens to HS can mitigate the disruptive effect of E. maxima on the ileal digestibility and absorption by limiting the parasite-induced tissue injury and suppressing the enterocytic inducible oxidative damage.


Assuntos
Eimeria , Transtornos de Estresse por Calor , Ração Animal/análise , Fenômenos Fisiológicos da Nutrição Animal , Animais , Galinhas/genética , Dieta/veterinária , Suplementos Nutricionais , Digestão , Eimeria/genética , Transtornos de Estresse por Calor/metabolismo , Resposta ao Choque Térmico , Íleo/metabolismo , Carne , Nutrientes , RNA Mensageiro/genética , RNA Mensageiro/metabolismo
3.
Mymensingh Med J ; 30(4): 903-906, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34605454

RESUMO

Retrocalcaneal bursitis is one of the important causes of posterior heel pain which is due to repetitive friction of the retrocalcaneal bursa between the postero-superior calcaneal tuberosity (haglund deformity) and Achilles tendon. Most of the patients are treated by conservative methods. But when the condition becomes chronic and not responding to the conservative treatment, surgical treatment is an option of these cases. This prospective interventional study was conducted in the Department of Orthopaedic Surgery, BSMMU (Bangabandhu Sheikh Mujib Medical University), Dhaka, Bangladesh from September 2017 to August 2019. Within this period, total 40 cases of chronic retrocalcaneal bursitis were operated at BSMMU. The clinical and functional outcome was evaluated according to AOFAS (American Orthopedic Foot Ankle Society) scale. The results of this study showed significantly improvement of pain, functional status and deformity at the time of final follow-up period of 1 year, total mean score improved from 46/100 to 89/100. The outcome of the subjects was satisfactory 90% and unsatisfactory 10%. Surgical intervention is an effective option of treatment with satisfactory outcome, in the treatment of chronic retrocalcaneal bursitis.


Assuntos
Tendão do Calcâneo , Bursite , Calcâneo , Bangladesh , Bursite/cirurgia , Calcâneo/diagnóstico por imagem , Calcâneo/cirurgia , Humanos , Estudos Prospectivos , Resultado do Tratamento
4.
Br J Biomed Sci ; 78(2): 63-66, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32569501

RESUMO

BACKGROUND: Behçet's disease is a chronic relapsing and remitting autoimmune multisystem inflammatory disease characterised by oral aphthae, genital ulcers, skin lesions, gastrointestinal involvement, arthritis, vascular lesions and neurological manifestations. We hypothesised a link between rs57095329 of miR-146a and Behçet's disease, with further links with common clinical features. METHODS: We tested our hypothesis in 130 Behçet's disease patients and 131 age and sex-matched healthy controls. Behcet's disease current activity index (BDCAI) was used to assess patients' disease activity status. MiR-146a (rs57095329) was genotyped in all participants using RT-PCR and results in patients analysed according to clinical features. RESULTS: The frequency of the GG and AG genotypes in rs57095329 were strongly associated with Behçet's disease (adjusted OR 8.05, 95% CI 3.63-17.82; P < 0.001 and OR 2.26, 95% CI 1.27-4.04; P = 0.006, respectively), and in dominant (GG+AG > AA) and recessive (GG > AA+AG) models (both P < 0.001). Additionally, G allele distribution was significantly greater in Behçet's disease compared with controls (OR 2.85, 95% CI 1.98-4.11, P < 0.001). The AA genotype and A allele were linked to oral ulcers, the GG genotype and G allele to neurological disease, and the GG genotype and G allele to ocular disease (all P < 0.01). There were no links with genital ulceration, skin lesions, vascular disease or the result of the pathergy test. CONCLUSION: The miR-146a (rs57095329) is associated with Behçet's disease and certain genotypes and alleles with oral ulcers, and with ocular and neurological manifestations.


Assuntos
Síndrome de Behçet , MicroRNAs , Alelos , Síndrome de Behçet/genética , Genótipo , Humanos , MicroRNAs/genética , Polimorfismo de Nucleotídeo Único/genética
5.
Sci Rep ; 10(1): 10736, 2020 07 01.
Artigo em Inglês | MEDLINE | ID: mdl-32612102

RESUMO

Coccidiosis, caused by Eimeria spp. presents a self-limiting intestinal infection of poultry. Intestinal replication of the parasite causes severe morphological alterations to the host gastrointestinal tract, marked, among others, by the disruption of the intestinal barrier. We have previously reported a significant reduction in merozoite replication and oocyst shedding in E. tenella in vitro and in vivo. The objective of this study was to investigate the pathogenesis of E. maxima infection in broiler chickens under heat stress (HS) and mRNA expression of host cytokines that might affect the curtailed development of the parasite. We herein demonstrate that there is a significant detrimental effect of HS on the pathogenesis of E. maxima infection in broilers. There was a restricted replication of the parasite in HS chickens evidenced by significantly reduced oocyst shedding and disruption of the intestinal blood barrier. Gene expression of parasite genes demonstrated curtailed sexual reproduction of E. maxima in HS chickens. There was downregulation of Eimeria spp. genes related to gamete fusion, oocyst shedding, mitosis and spermiogenesis. Host gene expression indicates alterations in the cytokine expression that could be related to reduced parasite development in vivo.


Assuntos
Galinhas/parasitologia , Coccidiose/veterinária , Eimeria/fisiologia , Transtornos de Estresse por Calor/complicações , Oocistos/fisiologia , Doenças das Aves Domésticas/parasitologia , Desenvolvimento Sexual , Animais , Coccidiose/parasitologia , Eimeria/crescimento & desenvolvimento , Eimeria/patogenicidade , Resposta ao Choque Térmico , Intestinos/parasitologia , Carne/análise
6.
J Vector Borne Dis ; 57(3): 226-233, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-34472506

RESUMO

BACKGROUND & OBJECTIVES: Babesiosis is a tick transmitted disease, infecting a wide variety of wild and domestic animals, as well as humans. This study was designed to investigate molecular diagnosis and clinic-hemato-biochemical and oxidant/antioxidant status in dogs of Mizoram, India. METHODS: A total 1200 dogs screened for babesiosis during 2017-18 and 53 dogs suspected for babesiosis by clinical signs and were confirmed by molecular diagnosis. Clinical signs were recorded; also blood samples were taken to investigate hematologic changes, serum biochemical variations and oxidative stress biomarkers. RESULTS: The overall incidence of babesiosis in dogs of Aizawl, Mizoram, India during the study period recorded was 1.25% (15/1200) and 28.3% cases confirmed from 53 suspected dogs (15/53). The most commonly observed clinical signs were fever, emaciation, depression and icterus and lymphadenopathy. Significant reduction in PCV, HB, RBCs, MCHC, total protein, and albumin along with significant increase in MCV, WBCs, monocytes and BUN were the most consistent hemato-biochemical changes. Oxidant/antioxidant assessment showed significant reduction in superoxide dismutase, catalase and total anti-oxidant (TAC) along with significant increase in lipid peroxidase (LPO) activities. INTERPRETATION & CONCLUSION: The findings of this study demonstrated that the main causative agent of babesiosis in dogs in Mizoram Province is Babesia gibsoni which caused significant alteration of hemato-biochemical and oxidant-antioxidant status in dogs.


Assuntos
Babesia , Babesiose , Doenças do Cão , Animais , Antioxidantes , Babesiose/diagnóstico , Biomarcadores , Doenças do Cão/diagnóstico , Cães , Oxidantes
7.
Vet World ; 9(4): 342-5, 2016 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-27182126

RESUMO

AIM: The aim was to evaluate the estrus response, incidence of accessory corpus luteum formation and fertility following different hormonal protocols in repeat breeding crossbred cows. MATERIALS AND METHODS: This study was carried out on 24 repeat breeding crossbred cows allotted into four groups. Cows of Group I was not given any treatment, Group II was treated with gonadotropin-releasing hormone (GnRH) injection on day 6 post-estrus, Group III was treated with Ovsynch protocol, and Group IV was treated with Ovsynch based GnRH treatment. Estrus responses such as duration, onset, percentage, and intensity of estrus were recorded during the study. The incidence of accessory corpus luteum was recorded per rectally on day 7 after first and additional GnRH of Ovsynch treatment. The conception rate for all groups was calculated by the absence of estrus and on day 45 after artificial insemination (AI) per rectum. Serum samples were collected at AI and day 12 post-AI in Group I and II. Serum samples were also collected at GnRH, Prostaglandin F2α (PGF2α), timed AI (TAI) and day 12 post-TAI in Group III and IV. RESULTS: Ovsynch and Ovsynch based GnRH treatments are resulted in 100.00% induction of estrus after the PGF2α injection. Onset of induced estrus after the PGF2α injection for Group III and IV was recorded as 48.750±0.713 and 51.472±1.989 h, respectively, and it was not significant. There was no significant difference in duration of estrus among the groups. The incidence of intermediate estrus intensity was found to be highest. All the cows showed the incidence of formation of accessory corpus luteum subsequent to GnRH treatment on day 6 of the estrous cycle in Group II, III, and IV. The conception rate was 0.00%, 16.67%, 50.00%, and 50.00% in Group I, II, III, and IV, respectively. CONCLUSION: Ovsynch and Ovsynch based GnRH treatments initiated on day 6 of estrous cycle capable of responding with a higher percentage of ovulation and formation of accessory corpus luteum which helped in higher conception rate over single post-AI GnRH treatment in repeat breeders. These treatments responded with better estrus response but did not significantly improve estrus intensity.

8.
Bioorg Khim ; 41(1): 102-11, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26050478

RESUMO

A series of substituted (pyridin-4-yl)phenyl-2-methoxybenzamide and their derivatives were prepared and screened for their anti-inflammatory activities. Initially the acute toxicity of the compounds was assayed via the determination of their LD50. Some of the newly synthesized compounds exhibited better pharmacological and biological responses than the reference controls with low concentrations. The structures of newly synthesized compounds were confirmed by chemical, elemental and spectroscopic evidences.


Assuntos
Anti-Inflamatórios não Esteroides , Benzamidas , Animais , Anti-Inflamatórios não Esteroides/síntese química , Anti-Inflamatórios não Esteroides/química , Anti-Inflamatórios não Esteroides/farmacologia , Benzamidas/síntese química , Benzamidas/química , Benzamidas/farmacologia , Masculino , Estrutura Molecular , Ratos
9.
Arab J Gastroenterol ; 16(1): 14-9, 2015 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-25817827

RESUMO

BACKGROUND AND STUDY AIMS: Complete surgical removal of the involved bowel segment in colorectal cancer is the most effective primary treatment. The main prognostic factors for colorectal cancer are penetration of the tumour into different layers of the bowel wall and regional lymph node involvement. Positive lavage cytology has been used to predict peritoneal recurrence, but its effectiveness remains controversial. This study was conducted to assess the prevalence of positive peritoneal lavage cytology in correlation with the tumour stage in patients with colorectal cancer. PATIENTS AND METHODS: This prospective cross-sectional study was performed on 20 patients with different cases of colorectal cancer attending the colorectal unit and emergency department of the Kasr Al Ainy Hospital, Cairo University Hospitals, from March 2012 to March 2013. RESULTS: The patients' gender did not influence the peritoneal lavage cytology results (p = 0.062); there is no significant correlation between the TNM staging system and cytology in patients with colorectal cancer (p = 0.253). CONCLUSION: Although there is a positive linear correlation between the tumour stage and positive peritoneal lavage cytology, it did not reach a statistically significant level. In addition, the greater the depth of invasion, the higher the lavage cytology rate. However, this trend was not statistically significant.


Assuntos
Neoplasias Colorretais/patologia , Citodiagnóstico/métodos , Lavagem Peritoneal , Fatores Etários , Idoso , Biópsia por Agulha , Neoplasias Colorretais/diagnóstico , Estudos Transversais , Egito , Feminino , Humanos , Imuno-Histoquímica , Masculino , Pessoa de Meia-Idade , Invasividade Neoplásica/patologia , Estadiamento de Neoplasias , Cavidade Peritoneal/citologia , Valor Preditivo dos Testes , Prognóstico , Estudos Prospectivos , Medição de Risco , Sensibilidade e Especificidade , Fatores Sexuais
10.
Res Vet Sci ; 93(2): 604-10, 2012 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-22226073

RESUMO

Thirty-one shiga toxin-producing (STEC) and 6 enteropathogenic Escherichia coli (EPEC) were isolated from 87 raw yak milk and 63 'churpi' samples. Of 18 stx(1) positive isolates (48.6%), 14 carried stx(1c) (77.7%). Subtyping of 28 stx(2) positive isolates (75.7%) revealed the presence of stx(2c) (9, 32.1%), stx(2d) (3, 10.7%), stx(2e) (1, 3.57%) and stx(2f) (3, 10.7%) variants. Furthermore, intimin (eaeA), enterohaemolysin (ehxA), autoagglutinating adhesin (saa), iha (adherence conferring protein), efa1 (EHEC factor for adherence), bundle forming pilli (bfpA) and toxB (type III secreted protein encoded on LEE Island, similar to toxin B of Clostridium difficile) genes were detected in 14, 16, 12, 4, 3, 2 and 2 isolates, respectively. Univariate and multivariate analysis depicted that both stx(1) and stx(2) or their variants were more likely to occur in isolates from Arunachal Pradesh (p<0.04) rather than Sikkim. Dendogram constructed on the basis of RAPD and ERIC PCR profile distributed the STEC and EPEC isolates in separate clusters irrespective of their sources and serotypes. The STEC and EPEC isolates exhibited resistance against erythromycin, amikacin, azithromycin, amoxicillin, ampicillin+cloxacillin, cephalothin, furazolidone, gentamicin, kanamycin, streptomycin and tetracycline. This is the first ever report on occurrence and characterization of STEC and EPEC isolated from yak milk and milk products.


Assuntos
Queijo/microbiologia , Escherichia coli Enteropatogênica/isolamento & purificação , Microbiologia de Alimentos , Leite/microbiologia , Escherichia coli Shiga Toxigênica/isolamento & purificação , Animais , Bovinos , Proteínas de Escherichia coli/genética , Proteínas de Escherichia coli/metabolismo , Feminino , Razão de Chances
11.
Anim Reprod Sci ; 104(2-4): 299-305, 2008 Mar 03.
Artigo em Inglês | MEDLINE | ID: mdl-17383122

RESUMO

The objective of this study was to test the efficacy of induction of estrus, synchronization of ovulation and timed artificial insemination in anestrous yaks using the Heatsynch protocol. In Experiment 1, 10 anestrous yaks were administered an analogue of gonadotropin releasing hormone (GnRH) followed by prostaglandin (PG)F2alpha 7 days later and then estradiol cyponate (ECP) 24 h after that. Ovulation was detected by rectal palpation at 2h intervals beginning at the initial signs of estrus. Blood samples were collected at 2h intervals beginning at the time of ECP injection up to 2h after the occurrence of ovulation for the determination of LH and progesterone. All the animals responded to the Heatsynch protocol with expression of estrus and synchronization of ovulation. The mean time interval from the ECP injection to ovulation was 59.4+/-2.62 h (range 50-72 h). The interval from the LH peak to ovulation was 30.2+/-2.3 h. The high degree of synchrony in ovulation could be attributed to the synchrony in the timing of LH peaks. In Experiment 2, 10 anestrous yaks were treated with the Heatsynch protocol (as in Experiment 1) and TAI was performed at 48 and 60 h after the ECP treatment. Concurrently, 16 cycling yaks were inseminated approximately 12 h after detection of spontaneous estrus. Pregnancy rates were similar in both groups, 40% for TAI and 43.75% for yaks inseminated following spontaneous estrus (p>0.05). From this study, two conclusions can be drawn. First, the Heatsynch protocol can be successfully used to induce and synchronize estrus in anestrous yaks and, second, ovulation following the Heatsynch protocol is synchronized adequately to permit the use of fixed time AI in this species.


Assuntos
Bovinos/fisiologia , Sincronização do Estro/métodos , Inseminação Artificial/veterinária , Indução da Ovulação/veterinária , Animais , Busserrelina/farmacologia , Dinoprosta/farmacologia , Estradiol/análogos & derivados , Estradiol/farmacologia , Feminino , Fármacos para a Fertilidade Feminina/farmacologia , Inseminação Artificial/métodos , Inseminação Artificial/normas , Hormônio Luteinizante/sangue , Masculino , Indução da Ovulação/métodos , Indução da Ovulação/normas , Gravidez , Progesterona/sangue
12.
Ann Chim ; 97(3-4): 213-25, 2007.
Artigo em Inglês | MEDLINE | ID: mdl-17822229

RESUMO

Novel PVC membrane electrodes for the determination of betaine ion based on the formation of betaine-tetraphenylborate (Be-TPB) and betaine-phosphotungstate (Be-PT) ion-exchangers as electroactive materials are described. The sensors show a fast, stable, near Nernstian response for 6.92 x 10(-6) to 7.94 x 10(-3) M and 1.0 x 10(-4) to 1.0 x 10(-2) M betaine hydrochloride (Be.Cl) in case of Be-TPB electrode applying batch and flow injection analysis (FIA), respectively, and 2.95 x 10(-5) to 2.26 x 10(-3) M and 3.16 x 10(-5) to 1.0 x 10(-2) M in case of Be-PT electrode for batch and FIA electrodes, respectively, at 25 degrees C over the pH range of 3.5-10 with a cationic slope of 60.2 and 59.1 mV decade(-1) and a fast potential response of < or =15 s. The lower detection limits are 7.94 x 10(-6) and 3.18 x 10(-5) M Be.Cl for Be-TPB and Be-PT electrodes, respectively. Selectivity coefficient data for some common inorganic cations, sugars, amino acids and the components other than betaine, of the mixed drug investigated show negligible interference. The electrodes have been applied to the direct potentiometric determination of betaine hydrochloride in water and in a pharmaceutical preparation under batch and FIA conditions. Potentiometric titrations of Be.Cl with NaTPB and PTA as titrants were monitored with the developed betaine electrodes as an end point indicator electrode. The determination of Be.Cl shows an average recovery of 100.8% with mean relative standard deviation of 0.61%. The effect of temperature on the electrodes was also studied.


Assuntos
Betaína/análise , Potenciometria/métodos , Eletrodos , Concentração de Íons de Hidrogênio , Troca Iônica , Cloreto de Polivinila , Potenciometria/normas , Soluções , Temperatura , Titulometria
13.
Farmaco ; 60(5): 419-24, 2005 May.
Artigo em Inglês | MEDLINE | ID: mdl-15910814

RESUMO

Two sensitive spectrophotometric and atomic absorption spectrometric procedures have been developed for determination of cinchocaine hydrochloride (Cin.Cl) in pure form and in pharmaceutical formulation. The spectrophotometric method was based on formation of an insoluble colored ion-associate between the cited drug and tetrathiocyanatocobaltate (CoTC) or hexathiocyanatochromate (CrTC) which dissolved and extracted in an organic solvent. The optimal experimental conditions for quantitative extraction such as pH, concentration of the reagents and solvent were studied. Toluene and iso-butyl alcohol proved to be the most suitable solvents for quantitative extraction of Cin-CoTC and Cin-CrTC ion-associates with maximum absorbance at 620 and 555 nm, respectively. The optimum concentration ranges, molar absorptivities, Ringbom ranges and Sandell sensitivities were also evaluated. The atomic absorption spectrometric method is based on measuring of the excess cobalt or chromium in the aqueous solution, after precipitation of the drug, at 240.7 and 357.9 nm, respectively. Linear application ranges, characteristic masses and detection limits were 57.99-361.9, 50.40 and 4.22 microg ml(-1) of Cin.Cl, in case of CoTC, while 37.99-379.9, 18.94 and 0.81 microg ml(-1) in case of CrTC.


Assuntos
Dibucaína/análise , Espectrofotometria Atômica/métodos , Química Farmacêutica/métodos , Concentração de Íons de Hidrogênio , Pomadas , Preparações Farmacêuticas/química , Solventes/química , Solventes/classificação
14.
Farmaco ; 60(6-7): 541-6, 2005.
Artigo em Inglês | MEDLINE | ID: mdl-15890349

RESUMO

Sodium tetraphenylborate and phosphotungstic acid were used as titrants for the conductimetric determination of phenylpropanolamine HCl (PPA.Cl), ranitidine HCl (Ra.Cl), hyoscyamine HBr (Hy.Br) and betaine HCl (Be.Cl) through ion-associate complex formation. The molar combining ratio and the solubility products of the formed ion-associates were studied and calculated. The suggested method has been applied to the determination of the mentioned drugs in their pure state and pharmaceutical preparations with mean recovery values of 97.71-102.97% and relative standard deviations 0.25-0.85%. The accuracy of the method is indicated by excellent recovery and low standard deviation. The results are compared with the pharmacopoeial or the official methods.


Assuntos
Atropina/análise , Betaína/análise , Preparações Farmacêuticas/química , Fenilpropanolamina/análise , Ranitidina/análise , Cápsulas , Eletroquímica/instrumentação , Eletroquímica/métodos , Reprodutibilidade dos Testes , Solubilidade
15.
Arch Pharm (Weinheim) ; 335(6): 251-61, 2002 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-12210767

RESUMO

Condensation of carbohydrazide derivatives Ia, b with dimethyl acetylenedicarboxylate and acetylenedicarboxylic acid yielded benzofuran derivatives II a-d. Reaction of Ib with aromatic aldehydes formed products III a-d. Treatment of compounds III a-d with mercaptoacetic acid yielded the cyclocondensation products (IVa-d). Phthalic anhydride reacted with compounds (Ia, b)to form products (Va, b). It has been found that both khellin and visnagin (VIa, b)react with aromatic aldehydes to give arylidene derivatives (VIIa-e). Condensation of diphenyl nitrilamine with 2-arylidene furochromones VII derivatives afforded cyclo-adducts (VIII a-i). The antibacterial activities of the selected compounds were tested against Staphylococcus aureus, B. subtilis, E. coli, Pseudomonas, Salmonella and Erwinia with good results.


Assuntos
Antibacterianos/síntese química , Antibacterianos/farmacologia , Bactérias/efeitos dos fármacos , Oxazóis/síntese química , Oxazóis/farmacologia , Ftalimidas/síntese química , Ftalimidas/farmacologia , Tiazóis/síntese química , Tiazóis/farmacologia , Fenômenos Químicos , Físico-Química , Indicadores e Reagentes , Quelina/química , Espectroscopia de Ressonância Magnética , Testes de Sensibilidade Microbiana , Espectrofotometria Infravermelho
16.
Brain Res ; 913(2): 133-9, 2001 Sep 21.
Artigo em Inglês | MEDLINE | ID: mdl-11549376

RESUMO

Brimonidine, a selective alpha(2)-adrenoceptor agonist, has recently been shown to be neuroprotective as it significantly improves survival of retinal ganglion cells (RGCs) after calibrated optic nerve injury in rats. In the present study, we examined the effect of brimonidine (alpha(2)-adrenoceptor agonist) on RGC survival after increased intraocular pressure (IOP) in adult rats. RGCs were prelabeled by bilateral tectal injection of 5% Fluoro-Gold (FG). Two days later, unilaterally IOP was increased 2.2-2.5 times (28-30.5 mmHg) that of the normal pressure (12.5-14.5 mmHg) by cauterization of three episcleral veins. The elevated IOP was maintained throughout the duration of the experiment. Rats were treated intraperitoneally with brimonidine (1 mg/kg) or phosphate-buffered saline (PBS) once per week beginning either before (group A) or after (group B) increasing the IOP. Another group of rats was left as the control with elevated IOP but without any brimonidine/PBS treatment. Rats were euthanized at 3, 4 and 5 weeks after IOP elevation. Identifiable RGCs were counted and compared between control and experimental groups. Brimonidine significantly protected RGCs from elevated IOP-induced cell death. In control rats with three-vein cauterization, there was 5-6% cell death per week. Almost all RGCs were protected following brimonidine treatment for 3 weeks both in groups A and B. At 4 weeks, there was 4.5% cell death in group A and 6.5% in group B. At 5 weeks, cell death was 5.9% in group A and 6.2% in group B. The difference in cell death in groups A and B was insignificant. No significant differences were observed between PBS-treated and control groups. No significant changes in elevated IOP was found after brimonidine or PBS treatment when compared with the nontreated control group. Although pressure remained elevated throughout the length of the experiment, 3 weeks later the amount of cell death gradually increased in brimonidine-treated animals.


Assuntos
Antagonistas Adrenérgicos alfa/farmacologia , Glaucoma/tratamento farmacológico , Pressão Intraocular/efeitos dos fármacos , Degeneração Neural/tratamento farmacológico , Fármacos Neuroprotetores/farmacologia , Quinoxalinas/farmacologia , Células Ganglionares da Retina/efeitos dos fármacos , Fatores Etários , Animais , Tartarato de Brimonidina , Contagem de Células , Sobrevivência Celular/efeitos dos fármacos , Sobrevivência Celular/fisiologia , Modelos Animais de Doenças , Feminino , Glaucoma/patologia , Glaucoma/fisiopatologia , Pressão Intraocular/fisiologia , Degeneração Neural/patologia , Degeneração Neural/fisiopatologia , Ratos , Ratos Wistar , Células Ganglionares da Retina/metabolismo , Células Ganglionares da Retina/patologia , Fatores de Tempo
17.
Biochim Biophys Acta ; 1535(2): 192-9, 2001 Feb 14.
Artigo em Inglês | MEDLINE | ID: mdl-11342008

RESUMO

The molecular response during recovery from torsion-induced stress in the testis is diverse with a variety of mechanisms. In this study, using unilateral testicular torsion in rat as a model, we used subtractive hybridisation to identify differentially expressed DNA sequences in the torsioned and control testes. Three genes were identified as being down regulated in the torsioned testis compared with controls: Control Testis genes 1, 2 and 3 (CT1, CT2 and CT3). Two genes were up regulated in the torsioned testes: Torsioned Testes genes 1 and 2 (TT1 and TT2). Differential expression was confirmed by Reverse Northern blot analysis. An homology search revealed that CT1 had 88% homology with rat metallothionein cDNA; CT2 had 81% homology with rat cell surface antigen in MHC class I, but no homology could be found for CT3. TT1 had 92% identity with rat Rieske iron-sulphur protein mRNA whereas TT2 had 73% identity with a human clone of unknown function (RP 11-252D22). These results indicate that changes in gene expression occur following torsion induced stress, and that identification of differentially expressed genes may provide insights into the mechanisms of cellular tissue damage in this model.


Assuntos
DNA Complementar/análise , Complexo III da Cadeia de Transporte de Elétrons , Torção do Cordão Espermático/fisiopatologia , Animais , Expressão Gênica , Proteínas Ferro-Enxofre/genética , Proteínas Ferro-Enxofre/metabolismo , Masculino , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Ratos , Ratos Wistar , Homologia de Sequência , Torção do Cordão Espermático/genética , Torção do Cordão Espermático/metabolismo , Testículo/metabolismo , Testículo/patologia
18.
Int J Dev Neurosci ; 19(2): 209-18, 2001 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-11255034

RESUMO

The effects of elevated intraocular pressure (IOP) on the morphology of rat retinal ganglion cells (RGCs) was analyzed in this study. After cauterizing two limbal derived episcleral veins, IOP in experimental eyes was elevated 1.5--1.8 times that of control. RGCs of experimental and control eyes were analyzed after: bilateral tectal injections of Fluoro-Gold, and application of fluorescent dye crystals, 4-Di-10-ASP to the proximal stump of the cut optic nerve, at different time intervals after IOP elevation. The RGCs in control and experimental eyes were evaluated at 4, 6, 8, and 10 weeks by counting, as well as by determining the soma diameter. The dendritic field of three types (I, II, III) of RGCs between control and experimental eyes were also studied at 4,6,10 weeks after IOP elevation. At every time point, the number of cells in experimental eyes were significantly less than those of the control eyes. The average retinal ganglion cell death was 3--4% per week in the eyes with elevated IOP. The soma and dendritic field diameter of the RGCs in the experimental eyes were significantly larger in all cell types. However, types I and III cells expanded their dendritic fields more rapidly than type II cells. Furthermore, dendritic fields of surviving RGCs in experimental eyes occupied about the same extent of the retina as the controls. The increase in soma diameter and expansion of dendritic fields in the remaining RGCs in eyes with elevated IOP suggests the existence of plasticity in adult retina.


Assuntos
Hipertensão Ocular/patologia , Células Ganglionares da Retina/patologia , Estilbamidinas , Animais , Tamanho Celular , Dendritos/patologia , Feminino , Corantes Fluorescentes , Pressão Intraocular , Microscopia de Fluorescência , Plasticidade Neuronal , Ratos
19.
Exp Neurol ; 167(2): 451-5, 2001 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-11161634

RESUMO

Injury to axons in the CNS leads to little regenerative repair and loss of function. Conversely, injury to axons in the PNS results in vigorous regrowth of severed axons, usually with restoration of function. This difference is generally attributed to a CNS environment that either cannot support or actively inhibits regeneration and/or a failure of CNS neurons to survive axotomy. One of the earliest responses of neurons to axotomy is the resealing of cut axons. A delay in resealing could affect a neuron's ability to survive axotomy and to regenerate a new axon. In the present experiments, using a dye exclusion technique, we demonstrate that following transection of a peripheral sensory nerve, axons reseal within 8--10 h, whereas following optic nerve transection complete resealing does not occur for more than 20 h. These results show that resealing of cut axons in a CNS environment is significantly delayed compared with axons in the PNS and suggest that this could contribute to the failure of CNS neurons to regenerate following injury.


Assuntos
Axônios/fisiologia , Sistema Nervoso Central/fisiologia , Regeneração Nervosa/fisiologia , Sistema Nervoso Periférico/fisiologia , Amidinas , Animais , Axotomia , Contagem de Células , Sistema Nervoso Central/citologia , Dextranos , Feminino , Corantes Fluorescentes , Gânglios Espinais/fisiologia , Masculino , Nervo Óptico/fisiologia , Sistema Nervoso Periférico/citologia , Ratos , Ratos Wistar , Células Ganglionares da Retina/citologia , Células Ganglionares da Retina/fisiologia , Rodaminas , Fatores de Tempo
20.
Int J Androl ; 23(6): 347-56, 2000 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-11114980

RESUMO

Male rats were subjected to 1 h testicular torsion of the spermatic cord or 1 h torsion followed by detorsion and recovery up to 4 weeks. The extent of tissue damage was evaluated by a testicular biopsy score count and mitochondrial function. Torsion for 1 h followed by detorsion induced significant morphological damage, which became more severe with longer periods of recovery. This morphological damage could not be correlated with mitochondrial damage as assessed by measuring the 4834 bp mitochondrial DNA 'common deletion' using a quantitative competitive polymerase chain reaction (PCR) assay. Mitochondrial respiratory chain activity, as measured by mitochondrial oxygen consumption using an oxygen electrode, did not vary between the treated animals and the controls. We conclude that the common mitochondrial DNA deletion and oxygen consumption are not good indicators of testicular damage induced by torsion.


Assuntos
Mitocôndrias/genética , Mitocôndrias/metabolismo , Mutação , Torção do Cordão Espermático/patologia , Testículo/fisiopatologia , Animais , Respiração Celular , DNA Mitocondrial/genética , Masculino , Oxigênio/metabolismo , Reação em Cadeia da Polimerase , Ratos , Ratos Wistar , Deleção de Sequência , Torção do Cordão Espermático/genética , Torção do Cordão Espermático/metabolismo , Testículo/metabolismo
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