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1.
J Appl Microbiol ; 126(4): 1278-1289, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30633843

RESUMO

AIMS: Cyanobacteria are immense sources of several pharmacological active compounds such as flavonoids and carotenoids with anti-inflammatory and antioxidant activity. The potential therapeutic effect of two novel cyanobacterial isolates, Cronbergia siamensis (KY296358.1) and Sphaerospermopsis aphanizomenoides (KU212886.1), against hydrogen peroxide (H2 O2 )-induced oxidative stress damage in the rat model was determined in this study. METHODS AND RESULTS: In vitro antioxidant activity of the two studied isolates was evaluated by radical scavenging assay and ferric reducing power. The possible prophylactic activity of S. aphanizomenoides (KU212886.1) against H2 O2 -induced oxidative stress in the rat model was assessed in vivo. Serum alanine transaminase and aspartate transaminase were measured for the liver functions in redox rats. Liver malondialdehyde (MDA), glutathione, oxidized glutathione, nitric oxide, superoxide dismutase (SOD) and catalase (CAT) were assessed as oxidative stress markers. The effect of S. aphanizomenoides on the transcripts level of superoxide dismutase (Mn-SOD) and catalase (CAT) genes in the rat's liver tissues was measured using qRT-PCR. Oral administration of S. aphanizomenoides extract in low and high doses (100, 200 mg kg-1 b.w) resulted in significant improvement in biochemical parameters of liver functions and oxidative stress markers. Also, the endogenous antioxidant defence enzymes and the expression of their related genes (Mn/SOD, CAT) were upregulated. Immunohistochemistry of Caspase-3, an apoptotic marker, showed potent amelioration in the liver tissues. CONCLUSIONS: The novel isolate S. aphanizomenoides proved in vitro and in vivo antioxidant activity against redox rat model. SIGNIFICANCE AND IMPACT OF THE STUDY: This isolate provides a new source of pharmacological compounds with great importance in pharmacological and medical fields.


Assuntos
Antioxidantes/uso terapêutico , Cianobactérias/fisiologia , Estresse Oxidativo/efeitos dos fármacos , Animais , Antioxidantes/administração & dosagem , Antioxidantes/metabolismo , Cianobactérias/isolamento & purificação , Modelos Animais de Doenças , Regulação Enzimológica da Expressão Gênica/efeitos dos fármacos , Peróxido de Hidrogênio/toxicidade , Fígado/efeitos dos fármacos , Fígado/metabolismo , Fígado/patologia , Ratos , Espécies Reativas de Oxigênio/metabolismo
2.
Andrologia ; 50(1)2018 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-28444774

RESUMO

Busulfan is an anticancer drug caused variety of adverse effects for patients with cancer. But it could cause damage to the male reproductive system as one of its adverse effects. This study aimed to investigate the protective effect of L-carnitine and L-arginine on semen quality, oxidative stress parameters and testes cell energy after busulfan treatment. Adult male rats were divided into four groups: control (Con), busulfan (Bus), busulfan plus L-arginine (Bus + L-arg) and busulfan plus L-carnitine (Bus + L-car). After 28 days, the semen was collected from the epididymis and the testes were assessed. Sperm count, motility and velocity were measured by CASA, and smears were prepared for assessment of sperm morphology. Serum and testes supernatants were separated for DNA metabolites, oxidative stress and cell energy parameters. Testes tissues also subjected for caspase-3. The results showed significant improvement in sperm morphology, motility, velocity and count in the groups treated with L-arginine and L-carnitine and accompanied with an increase in MDA, GSSG and ATP, reduction in GSH, AMP, ADP, NO and 8-OHDG also recorded. These results are supported by caspase-3. CONCLUSIONS: Administration of L-arg and L-car attenuated the cytotoxic effects of busulfan by improving semen parameters, reducing oxidative stress and maintaining cell energy.


Assuntos
Arginina/uso terapêutico , Bussulfano , Carnitina/uso terapêutico , Oligospermia/tratamento farmacológico , Espermatozoides/efeitos dos fármacos , Animais , Arginina/farmacologia , Carnitina/farmacologia , Caspase 3/metabolismo , Glutationa/metabolismo , Masculino , Malondialdeído/metabolismo , Oligospermia/induzido quimicamente , Oligospermia/metabolismo , Estresse Oxidativo/efeitos dos fármacos , Ratos , Contagem de Espermatozoides , Motilidade Espermática/efeitos dos fármacos , Espermatozoides/metabolismo , Resultado do Tratamento
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