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Polymers (Basel) ; 13(7)2021 Mar 25.
Artigo em Inglês | MEDLINE | ID: mdl-33805907


This study evaluated an experimental two-step self-etch adhesive (BZF-29, BZF) by comparing it with a reference two-step self-etch adhesive (Clearfil Megabond 2, MB) and a universal adhesive (G-Premio Bond, GP) for microtensile bond strength (µTBS) and resin-dentin interfacial characteristics. Twenty-four human third molars were used for the µTBS test. Bonded peripheral dentin slices were separated to observe the resin-dentin interface and measure the adhesive layer thickness with SEM. µTBS data of the central beams were obtained after 24 h and 6 months of water storage. Fracture modes were determined using a stereomicroscope and SEM. Nine additional third molars were used to determine the elastic modulus (E) employing an ultra microhardness tester. Water storage did not affect µTBS of the tested adhesives (p > 0.05). µTBS of BZF and MB were similar but significantly higher than GP (p < 0.05). BZF achieved the highest adhesive layer thickness, while GP the lowest. E of BZF and MB were comparable but significantly lower than GP (p < 0.05). Except for GP, the predominant fracture mode was nonadhesive. The superior bonding performance of BZF and MB could be attributed to their better mechanical property and increased adhesive thickness imparting better stress relief at the interface.

J Esthet Restor Dent ; 33(6): 874-883, 2021 09.
Artigo em Inglês | MEDLINE | ID: mdl-33188588


OBJECTIVE: To evaluate the shade matching ability of a novel supra-nano filled esthetic resin composite employing structural color technology using simplified simulated clinical cavities. Filler morphology and light transmittance characteristics were also evaluated. MATERIALS AND METHODS: One-hundred and twenty frames of resin composite were built in A1, A2, A3, and A4 shades to simulate Class I cavities (diameter = 4 mm, height = 2 mm). For each shaded frame, cavities were filled with three different types of filler containing resin composites (n = 10): supra-nano filled (SN filled) resin composite, microhybrid filled (MH filled) resin composite, and clustered-nano filled (CN filled) resin composite. Color parameters were calculated using CIELAB (△Eab ) and CIEDE2000 (△E00 ). Data were analyzed using one-way analysis of variance (ANOVA), followed by Duncan's test (α = .05). Filler morphology and light transmittance characteristics were measured to explore the role of structural color on shade matching. RESULTS: △Eab and △E00 of SN filled resin composite were significantly lower in A2, A3, and A4 shades (P < .05). CONCLUSIONS: The SN filled resin composite showed better shade matching with A2, A3, and A4 shades of resin composite frames compared to MH filled resin composite, and CN filled resin composite. CLINICAL SIGNIFICANCE: Universal-shade resin composites, which were expected to match nearly all shades, simplify the restorative procedure. Resin composite, which contained spherical supra-nano filler particles, could contribute most to its shade matching by stimulating structural color. Structural color technology may provide additional benefits for shade matching of resin composites.

Cárie Dentária , Estética Dentária , Cor , Resinas Compostas , Humanos , Teste de Materiais
Cancers (Basel) ; 12(5)2020 May 12.
Artigo em Inglês | MEDLINE | ID: mdl-32408515


Oncolytic virotherapy is a novel approach to cancer therapy. Ad-fosARE is a conditionally replicative adenovirus engineered by inserting AU-rich elements (ARE) in the 3'-untranslated region of the E1A gene. In this study, we examined the oncolytic activity of Ad-fosARE and used it in a synergistic combination with the chemotherapeutic agent paclitaxel (PTX) for treating cancer cells. The expression of E1A was high in cancer cells due to stabilized E1A-ARE mRNA. As a result, the efficiency of its replication and cytolytic activity in cancer cells was higher than in normal cells. PTX treatment increased the cytoplasmic HuR relocalization in cancer cells, enhanced viral replication through elevated E1A expression, and upregulated CAR (Coxsackie-adenovirus receptor) required for viral uptake. Furthermore, PTX altered the instability of microtubules by acetylation and detyrosination, which is essential for viral internalization and trafficking to the nucleus. These results indicate that PTX can provide multiple advantages to the efficacy of Ad-fosARE both in vitro and in vivo, and provides a basis for designing novel clinical trials. Thus, this virus has a lot of benefits that are not found in other oncolytic viruses. The virus also has the potential for treating PXT-resistant cancers.

Int J Mol Sci ; 20(21)2019 Oct 29.
Artigo em Inglês | MEDLINE | ID: mdl-31671751


The removal or modification of smear layers that cover the dentin is critical to allow the penetration of adhesive molecules and to ensure a strong bond between resin and dentin. Aiming to establish a model for clinically-relevant dentin-bond testing, we evaluated the effects of smear layers created by abrasives having similar coarseness (180-grit SiC paper; fine-grit diamond bur) and application modes (single application; double application) on the microtensile bond strengths (µTBS) of two currently available universal adhesives (G-Premio Bond; Scotchbond Universal Adhesive) and a two-step self-etch adhesive (Clearfil Megabond 2). Sixty extracted human third molars were used for the µTBS test. Data were analyzed by three-way ANOVA and Tukey's test (α = 0.05). Fracture modes were determined using stereomicroscopy. An additional 24 third molars were prepared for observation of the resin-dentin interface by TEM and adhesive-smear layer interaction by SEM. µTBS was significantly affected by the adhesives and their application modes (p < 0.001), implying that the double application of universal adhesives should be recommended to improve their performance. The effect of smear layers was not significant (p > 0.05), indicating that 180-grit SiC papers could be used to prepare dentin as a substitute for fine-grit diamond burs for dentin-bond testing in laboratory settings.

Adesivos/química , Colagem Dentária/métodos , Adesivos Dentinários/química , Camada de Esfregaço/química , Estresse Mecânico , Resistência à Tração , Resinas Compostas/química , Dentina/ultraestrutura , Humanos , Teste de Materiais , Microscopia Eletrônica de Transmissão , Dente Serotino , Cimentos de Resina , Propriedades de Superfície
J Adhes Dent ; 21(1): 59-66, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30799472


PURPOSE: To evaluate the effects of double application of self-etch adhesives on their microtensile bond strength (µTBS) to dentin, as well as the hardness (H) of resin-dentin interfacial structures. MATERIALS AND METHODS: Midcoronal dentin surfaces (n = 45) were polished with 180-grit SiC paper and randomly allocated to 9 groups based on three adhesives - Scotchbond Universal Adhesive (SB), G Premio Bond (GP), and Clearfil Megabond 2 (MB) - and three application modes: single application (S), double application with light curing after each application (DL), and double application with light curing only at the end (D). Following composite-resin build up and water storage (37°C; 24 h), µTBS were obtained. The hardness of adhesive, resin-dentin interface and dentin were evaluated by nanoindentation. The µTBS and H data were analyzed by two-way ANOVA to demonstrate the effects of adhesive and application mode as well as their interaction, followed by Tukey's test (α = 0.05). Fracture modes were determined using a stereomicroscope. RESULTS: µTBS and H were significantly affected by the adhesives and their application modes (p ˂ 0.001). Double application significantly increased the µTBS and H of adhesive and resin-dentin interface (p ˂ 0.05). SB and MB showed significantly higher µTBS than GP (p ˂ 0.05). CONCLUSION: Double applications during bonding of dentin having clinically relevant smear layers were beneficial for three contemporary self-etch adhesives. The improvement in bonding performance is believed to be the result of increased chemical interaction, better solvent removal, and improved resin infiltration, leading to improved mechanical properties of the resin-dentin interface or thicker adhesive layers providing improved stress distribution.

Colagem Dentária , Camada de Esfregaço , Cimentos Dentários , Dentina , Adesivos Dentinários , Humanos , Teste de Materiais , Cimentos de Resina , Resistência à Tração