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Animals (Basel) ; 11(11)2021 Oct 25.
Artigo em Inglês | MEDLINE | ID: mdl-34827779


Non-typeable (NT) Staphylococcus aureus strains are associated with chronic bovine mastitis. This study investigates the impact of biofilm formation by clinical NT S. aureus on cytokine production and mammary tissue damage by using a mouse mastitis model. Mice infected with two different NT S. aureus strains with strong and weak biofilm forming potential demonstrated identical clinical symptoms (moderate), minimal inflammatory infiltrates, and tissue damage (level 1 histopathological changes) in the mammary glands. However, the S. aureus load in the mammary glands of mice and the level of pro-inflammatory cytokines (IL-1ß, IL-6, IL-12, IL-17 and IFN-γ) in serum were significantly higher (p ≤ 0.05) in those infected with the strong biofilm forming NT S. aureus strain. The level of IL-6 in sera samples of these mice was extremely high (15,479.9 ± 532 Pg/mL). Furthermore, these mice died in 24h of post infection compared to 30 h in the weak biofilm forming NT S. aureus infected group. The study demonstrates no association between the strength of PIA (polysaccharide intercellular adhesion)-dependent biofilm production by clinical NT S. aureus and mammary gland pathology in a mouse mastitis model. However, the role of biofilm in the virulence of S. aureus advancing the time of mortality in mice warrants further investigation.

Pathogens ; 9(10)2020 Oct 19.
Artigo em Inglês | MEDLINE | ID: mdl-33086687


The objective of this study was to estimate the fecal carriage of Salmonella spp. among culled adult dairy cows presented to an abattoir in Wuhan, China and to evaluate their antimicrobial resistance profiles. Rectal swabs from 138 culled cows were cultured. Laboratory analysis involved the identification of Salmonella, the susceptibility assessment and the presence of Extended Spectrum ß-lactamases and mcr genes in the isolates. An overall prevalence of Salmonella of 29.0% was recorded with 63.4% (26/41) and 2.4% (1/41) of the isolates identified as S. Typhimurium and S. Dublin, respectively. The occurrence of Salmonella was higher (odd ratios: 3.3) in culled cows originating from the northeast zone of China than cows originating from the central and north zones. Twenty multi-drug resistant strains (resistant to three or more antimicrobial agents) were detected (48.8%) and overall, a high resistance to ampicillin (36/41) and tetracycline (15/41) was observed. Extended Spectrum ß-lactamases phenotypes were found in 7/41 isolates, of which all contained the blaCTX-M resistance gene, and no mcr genes were found by polymerase chain reaction. The high prevalence of Salmonella fecal carriage and antimicrobial resistance may contribute to an increased risk of Salmonella transmission to food.

Trop Anim Health Prod ; 42(8): 1643-7, 2010 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-20526675


The study reported data from 507 post-mortem records in the Department of Pathology, Microbiology and Parasitology, Faculty of Veterinary Medicine, University of Nairobi, Kenya. The records were from carcasses obtained from the peri-urban area of Nairobi during a 20-year period between 1990 and 2009. Approximately 80% (393/507) of the calf carcasses had their diagnosis made through post-mortem examination, while the rest (114/507) were inconclusive. Just less than half (48.3%) of the calf carcasses presented had their age specified by the owners compared to 51.7% whose age was not specified. For calf carcasses whose age was specified by the owners, those indicated as more than 3 months were one-and-a-half times as many as those below 3 months old. The proportion of female carcasses (53.8%, 273/507) presented for post-mortem were slightly higher than the male carcasses (46.2%, 234/507). Diseases or conditions of the respiratory system were the most common 17.7% (97/507) while gastrointestinal tract (GIT) was second and affected 16.1% (88/507) of the cases. Another small number, 3.3% (18/507), died from bloat giving the total cases associated with GIT as 19.4% (106/507). Severe calf malnutrition and septicaemia were the third most reported causes of calf mortality in similar proportions at 14.3% (78/507) and 14.4% (79/507), respectively. Other minor causes of calf mortality were tick-borne diseases 8.6% (47/507), helminthiasis and poisoning, 2.9% (16/507) and 1.8% (10/507), respectively.

Doenças dos Bovinos/epidemiologia , Doenças dos Bovinos/etiologia , Doenças dos Bovinos/mortalidade , Gastroenteropatias/veterinária , Desnutrição/veterinária , Transtornos Respiratórios/veterinária , Sepse/veterinária , Animais , Bovinos , Diagnóstico , Feminino , Gastroenteropatias/epidemiologia , Gastroenteropatias/mortalidade , Helmintíase Animal/epidemiologia , Helmintíase Animal/mortalidade , Quênia/epidemiologia , Masculino , Desnutrição/epidemiologia , Desnutrição/mortalidade , Transtornos Respiratórios/epidemiologia , Transtornos Respiratórios/mortalidade , Sepse/epidemiologia , Sepse/mortalidade , Doenças Transmitidas por Carrapatos/epidemiologia , Doenças Transmitidas por Carrapatos/mortalidade , Doenças Transmitidas por Carrapatos/veterinária