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1.
Braz. j. biol ; 82: e232868, 2022. tab, graf
Artigo em Inglês | LILACS-Express | LILACS | ID: biblio-1153458

RESUMO

Abstract The "piaussu", Megaleporinus macrocephalus is an anostomatid fish species native to the basin of the Paraguay River, in the Pantanal biome of western Brazil. However, this species has now been recorded in a number of other drainages, including those of the upper Paraná, Uruguay, Jacuí, Doce, Mucuri, and Paraíba do Sulrivers. This study presents two new records of the occurrence of M. macrocephalus, in the basins of the Itapecuru and Mearim rivers in the state of Maranhão, in the Brazilian Northeast. The piaussu is a large-bodied fish of commercial interest that is widely raised on fish farms, and its occurrence in the Itapecuru and Mearim rivers is likely the result of individuals escaping from fish tanks when they overflow during the rainy season. Morphological analyses and sequences of the Cytochrome Oxidase Subunit I (COI) gene confirmed the taxonomic identification of the specimens as M. macrocephalus. The COI sequences were 99.66% similar to those of M. macrocephalus deposited in the BOLDSystems database. These records extend the known distribution of M. macrocephalus to the basins of the Itapecuru and Mearim rivers in the Brazilian Northeast, highlighting a new case of introduction of exotic fish species into Brazilian river basins.


Resumo Megaleporinus macrocephalus é uma espécie de peixe anostomatídeo nativa da bacia do rio Paraguai, no bioma Pantanal do oeste do Brasil. No entanto, essa espécie já foi registrada em várias outras drenagens, incluindo as dos rios Alto Paraná, Uruguai, Jacuí, Doce, Mucuri e Paraíba do Sul. Este estudo apresenta dois novos registros da ocorrência de M. macrocephalus, nas bacias dos rios Itapecuru e Mearim, no estado do Maranhão, no nordeste brasileiro. O piaussu é um peixe de grande porte, de interesse comercial, amplamente criado em pisciculturas, e sua ocorrência nos rios Itapecuru e Mearim é provavelmente o resultado de indivíduos que escapam dos tanques quando transbordam durante a estação chuvosa. Análises morfológicas e sequências do gene da subunidade I do citocromo oxidase (COI) confirmaram a identificação taxonômica dos espécimes como M. macrocephalus. As sequências de COI foram 99,66% semelhantes às de M. macrocephalus depositadas no banco de dados BOLDSystems. Esses registros estendem a conhecida distribuição de M. macrocephalus às bacias dos rios Itapecuru e Mearim, no nordeste brasileiro, destacando um novo caso de introdução de espécies exóticas de peixes nas bacias hidrográficas brasileiras.

2.
Braz J Biol ; 82: e232868, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33681896

RESUMO

The "piaussu", Megaleporinus macrocephalus is an anostomatid fish species native to the basin of the Paraguay River, in the Pantanal biome of western Brazil. However, this species has now been recorded in a number of other drainages, including those of the upper Paraná, Uruguay, Jacuí, Doce, Mucuri, and Paraíba do Sulrivers. This study presents two new records of the occurrence of M. macrocephalus, in the basins of the Itapecuru and Mearim rivers in the state of Maranhão, in the Brazilian Northeast. The piaussu is a large-bodied fish of commercial interest that is widely raised on fish farms, and its occurrence in the Itapecuru and Mearim rivers is likely the result of individuals escaping from fish tanks when they overflow during the rainy season. Morphological analyses and sequences of the Cytochrome Oxidase Subunit I (COI) gene confirmed the taxonomic identification of the specimens as M. macrocephalus. The COI sequences were 99.66% similar to those of M. macrocephalus deposited in the BOLDSystems database. These records extend the known distribution of M. macrocephalus to the basins of the Itapecuru and Mearim rivers in the Brazilian Northeast, highlighting a new case of introduction of exotic fish species into Brazilian river basins.


Assuntos
Caraciformes , Rios , Animais , Brasil , Caraciformes/genética , Humanos , Paraguai , Uruguai
3.
Int J Oral Maxillofac Surg ; 48(9): 1201-1208, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-30871846

RESUMO

This study aimed to investigate the effects of bimaxillary advancement orthognathic surgery on the condylar remodeling of the temporomandibular joint (TMJ) using voxel-based regional superimposition of cone-beam computed tomography (CBCT). In this retrospective study, the sample comprised 56 condyles from 28 healthy patients (aged from 16 to 50years) with mandibular retrognathism treated with bimaxillary advancement. CBCT scans were taken preoperatively and at 14.3±4.2months postoperatively. The scans at the two time points were superimposed using regional voxel-based registration to assess condylar changes in the follow-up period. The linear alterations were measured in six different areas of each condyle to determine the pattern of condylar remodeling. Although no significant correlation was observed between changes in condylar surfaces, bone resorption occurred predominantly in the posterior and superior regions, while bone formation was predominantly on the anterior surface. Medial and lateral surfaces presented fewer bone changes. The overall bone changes were smaller than 1mm bilaterally in 21 patients (75%) and, considering each condyle individually, were smaller than 1mm in 48 condyles (85.7%). The results suggested that mild condylar remodeling in healthy patients is a common finding after orthognathic surgery. Future studies may clarify the mechanisms involved in the remodeling and help to understand the reasons for the remodeling pattern.


Assuntos
Cirurgia Ortognática , Procedimentos Cirúrgicos Ortognáticos , Adolescente , Adulto , Tomografia Computadorizada de Feixe Cônico , Humanos , Côndilo Mandibular , Pessoa de Meia-Idade , Estudos Retrospectivos , Articulação Temporomandibular , Adulto Jovem
4.
J Fish Biol ; 92(5): 1290-1311, 2018 May.
Artigo em Inglês | MEDLINE | ID: mdl-29532472

RESUMO

Uncertainty regarding the age determination of the Brazilian codling Urophycis brasiliensis has hampered its stock assessment. Transverse sections of otoliths displayed up to seven (in males) and 12 (in females) alternate opaque and translucent bands that could not be conclusively validated as annuli, resulting in unrealistically high ages of first maturity (A50 ) (A50male = 4·5 years and A50female = 6 years). Therefore, growth was described by the von Bertalanffy (VB) model using an alternative approach that combined microstructure data (daily growth increments) and a fixed asymptotic total length (L∞ ). This approach was supported by applying it to two other co-occurring species, the whitemouth croaker Micropogonias furnieri and the king weakfish Macrodon atricauda, for which daily and annual ring formation has previously been validated. The sensitivity to realistic errors associated with the choice of the L∞ and the daily increment readings was shown to be low. The results show that U. brasiliensis has a fast growth rate (Kmale = 1·19 year-1 , Kfemale = 0·71 year-1 ) and early maturation (A50male = 1·1-1·5 years, A50female  = 1·6-1·8 years); typical life-history traits for a sub-tropical coastal gadiform. This novel study offers an alternative approach for age and growth reconstruction for species with complex patterns of opaque and translucent bands provided that daily growth increments in the yearlings can be counted and L∞ reliably estimated.


Assuntos
Determinação da Idade pelo Esqueleto/métodos , Gadiformes/crescimento & desenvolvimento , Membrana dos Otólitos/crescimento & desenvolvimento , Animais , Tamanho Corporal , Brasil , Feminino , Gadiformes/anatomia & histologia , Masculino , Membrana dos Otólitos/anatomia & histologia , Perciformes/anatomia & histologia , Perciformes/crescimento & desenvolvimento , Maturidade Sexual
5.
Scand J Immunol ; 87(4): e12650, 2018 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-29473686

RESUMO

Immune homeostasis has been suggested to play an important role in the clinical evolution of chronic Chagas disease; however, the immunopathologic factors involved have not been fully elucidated. Therefore, our study aimed to analyse the frequency of CD4+ CD25+ FoxP3+ cells, classic Th17 cells, alternative Th17 cells and IL-17+ B cells from peripheral blood of chronic cardiac patients after in vitro stimulation with Trypanosoma cruzi soluble EPI antigen. Patients were selected and classified according to clinical evaluation of cardiac involvement: mild, B1 (CARD1) (n = 20) and severe, C (CARD2) (n = 11). Patients with the indeterminate form of CD were included as the control group A (IND) (n = 17). Blood samples were collected and cultured in the presence of EPI antigen. Cells frequency and median fluorescence intensity (MFI) were obtained by flow cytometry. Our results showed that only CD4+ CD25+ FoxP3+ , CD4+ CD25high FoxP3+ , CD4+ IL-17+ IFN-γ- and CD4+ IL-17+ IFN-γ+ cells are more frequent in patients with severe cardiac disease and correlate with worse global cardiac function. However, while indeterminate patients demonstrated a positive correlation between CD4+ CD25+ FoxP3+ and CD4+ IL-17+ IFN-γ- Th17 cells, this relationship was not observed in cardiac patients. IL-17 expression by Th17 cells and B cells correlated with disease progression. Altogether our results suggest that the clinical progression of Chagas cardiomyopathy involves worsening of inflammation and impairment of immunoregulatory mechanisms.


Assuntos
Subpopulações de Linfócitos B/imunologia , Cardiomiopatia Chagásica/patologia , Fatores de Transcrição Forkhead/metabolismo , Coração/fisiopatologia , Interleucina-17/metabolismo , Subunidade alfa de Receptor de Interleucina-2/metabolismo , Células Th17/imunologia , Subpopulações de Linfócitos B/classificação , Células Cultivadas , Feminino , Humanos , Inflamação/patologia , Interferon gama/imunologia , Masculino , Células Th17/classificação , Trypanosoma cruzi/imunologia , Função Ventricular Esquerda/fisiologia
6.
Genet Mol Res ; 15(3)2016 Aug 29.
Artigo em Inglês | MEDLINE | ID: mdl-27706636

RESUMO

DNA barcoding is a useful complementary tool for use in traditional taxonomic studies due to its ability to detect cryptic species, and may be particularly efficient in the identification of fish species. The fish fauna of the Itapecuru River represents an important fishery resource in the Brazilian State of Maranhão, although it is currently suffering increasing degradation as a result of anthropogenic impacts. Therefore, DNA barcoding was used in the present study to identify fish species and establish a database of the rich freshwater fish fauna of Maranhão. A total of 440 specimens were analyzed, corresponding to 64 species belonging to 59 genera, 31 families, and 10 orders. Overall, 92.19% of these species could be identified by DNA barcoding, and were characterized by low levels (average 0.80%) of intra-specific divergence. However, five species (Anableps anableps, Gymnotus carapo, Sciades couma, Pseudauchenipterus nodosus, and Leporinus piau) presented values of mean genetic divergence above 3%, indicating the existence of cryptic diversity in these fishes. The DNA barcoding approach permitted the analysis of a large number of specimens and facilitated the discrimination and identification of closely related fish species in the Itapecuru Basin.


Assuntos
Código de Barras de DNA Taxonômico/métodos , Complexo IV da Cadeia de Transporte de Elétrons/genética , Proteínas de Peixes/genética , Peixes/genética , Genoma , Filogenia , Animais , Brasil , DNA/genética , Peixes/classificação , Especiação Genética , Variação Genética , Rios
7.
Ann Hematol ; 95(2): 271-8, 2016 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-26518211

RESUMO

The introduction of agents such as thalidomide, lenalidomide, and bortezomib has changed the management of patients with multiple myeloma who are not eligible for autologous transplantation, many of whom are elderly. We sought to compare three thalidomide-based oral regimens among such patients in Latin America. We randomized patients with newly diagnosed multiple myeloma with measurable disease to one of the following regimens: melphalan, prednisone, and thalidomide (MPT); cyclophosphamide, thalidomide, and dexamethasone (CTD); and thalidomide and dexamethasone (TD). The TD arm was closed prematurely and was analyzed only descriptively. The primary endpoint was the overall response rate (ORR), whereas progression-free survival (PFS) and overall survival (OS) were secondary endpoints. The accrual rate was slower than expected, and the study was terminated after 82 patients had been randomized. The ORRs were 67.9 % with MPT, 89.7 % with CTD, and 68.7 % with TD (p = 0.056 for the comparison between MPT and CTD). The median PFS was 24.1 months for MPT, 25.9 months for CTD, and 21.5 months for TD. There were no statistically significant differences in PFS or OS between MPT and CTD. In an unplanned logistic regression analysis, ORR was significantly associated with treatment with CTD (p = 0.046) and with performance status of 0 or 1 (p = 0.035). Based on the current results, no definitive recommendations can be made regarding the comparative merit of the regimens tested. Nevertheless and until the results of further studies become available, we recommend either CTD or MPT as suitable frontline regimens for patients with multiple myeloma who are not candidates to transplantation in settings where lenalidomide and bortezomib are not available.


Assuntos
Protocolos de Quimioterapia Combinada Antineoplásica/administração & dosagem , Transplante de Células-Tronco Hematopoéticas , Mieloma Múltiplo/diagnóstico , Mieloma Múltiplo/tratamento farmacológico , Talidomida/administração & dosagem , Idoso , Intervalo Livre de Doença , Feminino , Humanos , Masculino , Transplante Autólogo
8.
Connect Tissue Res ; 56(1): 50-8, 2015 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-25360832

RESUMO

PURPOSE: Green tea (GT) is widely used due to its anti-inflammatory properties. Previous studies have shown beneficial effects of a glycine diet on the remodeling process in inflamed tendons. Tendinitis is commonly observed in athletes and is of concern to surgeons due to the slowness of the recovery process. Our hypothesis is that GT + a glycine diet may improve tendinitis. AIM OF THE STUDY: To analyze the effect of GT and/or glycine in the diet on tendinitis. MATERIALS AND METHODS: Wistar rats were divided into seven groups (G): control group (C); G1 and G4, tendinitis; G2 and G5, tendinitis supplied with GT; and G3 and G6, tendinitis supplied with GT and a glycine diet for 7 or 21 days, respectively. We performed zymography for metalloproteinase, biochemical, morphological and biomechanics tests. RESULTS: G2, G3 and G5 showed high levels of hydroxyproline in relation to G1, while G4 showed high levels of glycosaminoglycans. High activity of metalloproteinase-2 was detected in G3. The organization of collagen bundles was better in G2 and G3. G5 showed similar birefringence measurements compared with C. G5 withstood a larger load compared with G4. CONCLUSIONS: The presence of metalloproteinase-2 indicates that a tissue is undergoing a remodeling process. High birefringence suggests a better organization of collagen bundles. After 21 days, G5 sustained a high load before rupture, unlike G4. The results suggest that GT + a glycine diet has beneficial effects that aid in the recovery process of the tendon after tendinitis.


Assuntos
Tendão do Calcâneo/patologia , Glicina/uso terapêutico , Extratos Vegetais/uso terapêutico , Chá/química , Tendinopatia/tratamento farmacológico , Tendão do Calcâneo/efeitos dos fármacos , Animais , Fenômenos Biomecânicos/efeitos dos fármacos , Birrefringência , Forma Celular , Densitometria , Matriz Extracelular/efeitos dos fármacos , Matriz Extracelular/metabolismo , Glicina/farmacologia , Masculino , Metaloproteinases da Matriz/metabolismo , Extratos Vegetais/farmacologia , Ratos Wistar , Tendinopatia/patologia
9.
Food Chem Toxicol ; 77: 50-7, 2015 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-25544391

RESUMO

Cases of tendinopathy and tendon ruptures have been reported as side effects associated with statin therapy. This work assessed possible changes in the structural and biomechanical properties of the tendons after chronic treatment with statins. Wistar rats were divided into the following groups: treated with atorvastatin (A-20 and A-80), simvastatin (S-20 and S-80) and the group that received no treatment (C). The doses of statins were calculated using allometric scaling, based on the doses of 80 mg/day and 20 mg/day recommended for humans. The morphological aspect of the tendons in A-20, S-20 and S-80 presented signals consistent with degeneration. Both the groups A-80 and S-80 showed a less pronounced metachromasia in the compression region of the tendons. Measurements of birefringence showed that A-20, A-80 and S-80 groups had a lower degree of organization of the collagen fibers. In all of the groups treated with statins, the thickness of the epitenon was thinner when compared to the C group. In the biomechanical tests the tendons of the groups A-20, A-80 and S-20 were less resistant to rupture. Therefore, statins affected the organization of the collagen fibers and decreased the biomechanical strength of the tendons, making them more predisposed to ruptures.


Assuntos
Tendão do Calcâneo/efeitos dos fármacos , Tendão do Calcâneo/lesões , Colágeno/metabolismo , Inibidores de Hidroximetilglutaril-CoA Redutases/farmacologia , Traumatismos dos Tendões/patologia , Tendão do Calcâneo/anatomia & histologia , Animais , Atorvastatina/farmacologia , Relação Dose-Resposta a Droga , Masculino , Ratos , Ratos Wistar , Ruptura , Sinvastatina/farmacologia
10.
J Cutan Pathol ; 37(11): 1170-3, 2010 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-19615030

RESUMO

Systemic sclerosis (SSc) is an autoimmune systemic disease characterized by small vessel involvement that leads to tissue ischemia and fibroblast stimulation resulting in accumulation of collagen (fibrosis) in the skin and internal organs. Lipomembranous panniculitis is a peculiar type of fat necrosis and has been reported with clinical conditions, commonly with peripheral vascular diseases. We describe a case of a 43-year-old woman with SSc manifestations, who presented with black scaly skin plaques, associated with thickening of the subcutaneous fat tissue, on the lateral surface of her thighs, her calves, gluteal area and lower abdomen. Biopsy revealed lipomembranous panniculitis. Lipomembranous changes have been seen in connective tissue disorders such as lupus profundus, morphea, systemic sclerosis and panniculitis associated with dermatomyositis, but rarely in thighs, calves, gluteal area and lower abdomen.


Assuntos
Paniculite/etiologia , Escleroderma Sistêmico/complicações , Pele/patologia , Adulto , Feminino , Humanos , Paniculite/patologia , Escleroderma Sistêmico/patologia
11.
Plant Mol Biol ; 68(4-5): 321-35, 2008 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-18618271

RESUMO

Plant defensins are small (45-54 amino acids), highly basic, cysteine-rich peptides structurally related to defensins of other organisms, including insects and mammals. Small putative proteins (MW < 10 kDa) containing eight cysteines were screened based on the sugarcane expressed sequence tag (EST) database. We selected ORFs that exhibited 25-100% similarity in primary sequence with other defensins in the NCBI database and that contained eight cysteines. This similarity is sufficient for folding prediction, but not enough for biological activity inference. Six putative defensins (Sd1-6) were selected, and activity assays showed that recombinant Sd1, Sd3 and Sd5 are active against fungi, but not against bacteria. Structural characterization, based on circular dichroism (CD) and nuclear magnetic resonance (NMR) spectroscopy showed that the structures of these Sds were compatible with alpha/beta proteins, a feature expected for plant defensins. Phylogenetic analysis revealed that sugarcane defensins could clearly be grouped within defensins from Poaceae family and Andropogoneae tribe. Our work demonstrates that defensins show strong conservation in the Poaceae family and may indicate that the same conservation occurs in other families. We suggest that evolutionary relationships within plant families can be used as a procedure to predict and annotate new defensins in genomes and group them in evolutionary classes to help in the investigation of their biological function.


Assuntos
Defensinas/genética , Evolução Molecular , Poaceae/genética , Saccharum , Sequência de Aminoácidos , Antifúngicos/farmacologia , Cromatografia Líquida de Alta Pressão , Clonagem Molecular , Sequência Conservada , Defensinas/química , Defensinas/isolamento & purificação , Defensinas/farmacologia , Eletroforese em Gel de Poliacrilamida , Escherichia coli , Fungos/efeitos dos fármacos , Espectroscopia de Ressonância Magnética , Testes de Sensibilidade Microbiana , Dados de Sequência Molecular , Filogenia , Proteínas de Plantas/química , Proteínas de Plantas/genética , Proteínas de Plantas/isolamento & purificação , Proteínas de Plantas/farmacologia , Dobramento de Proteína , Proteínas Recombinantes/química , Proteínas Recombinantes/metabolismo , Alinhamento de Sequência
12.
Braz J Med Biol Res ; 40(2): 265-7, 2007 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-17273664

RESUMO

It is well established that interleukin-6 (IL-6) is an essential growth factor for multiple myeloma (MM) and patients with increased IL-6 levels have a poor prognosis. In healthy subjects, the presence of the C allele at a polymorphic site (-174 G/C) of the IL-6 gene is related to low IL-6 levels. In view of the potential association of this particular polymorphism with IL-6 concentration, and the relevance of IL-6 in MM pathogenesis, the objective of the present study was to investigate the prevalence of IL-6 (-174 G/C) promoter polymorphism and its association with development of MM in Brazilian individuals. We investigated the prevalence of these alleles in 52 patients and 60 healthy subjects (matched by age, sex, and race) of a Brazilian population. Thirty patients were male (42.4%), 24 (46.2%) were white and the median age at diagnosis was 58.5 years (range: 28 to 84 years). To determine the IL-6 (-174 G/C) polymorphism, molecular analysis was performed by polymerase chain reaction followed by endonuclease restriction digestion. The genotype distributions observed in the group of patients were 4% CC, 42% GC and 54% GG. The C allele frequency was 0.25. These results were similar to the control group, suggesting no impact of this polymorphism on the susceptibility to MM.


Assuntos
Interleucina-6/genética , Mieloma Múltiplo/genética , Polimorfismo Genético , Regiões Promotoras Genéticas/genética , Adulto , Idoso , Idoso de 80 Anos ou mais , Alelos , Feminino , Predisposição Genética para Doença , Genótipo , Humanos , Masculino , Pessoa de Meia-Idade , Reação em Cadeia da Polimerase
13.
Braz. j. med. biol. res ; 40(2): 265-267, Feb. 2007. tab
Artigo em Inglês | LILACS | ID: lil-440494

RESUMO

It is well established that interleukin-6 (IL-6) is an essential growth factor for multiple myeloma (MM) and patients with increased IL-6 levels have a poor prognosis. In healthy subjects, the presence of the C allele at a polymorphic site (-174 G/C) of the IL-6 gene is related to low IL-6 levels. In view of the potential association of this particular polymorphism with IL-6 concentration, and the relevance of IL-6 in MM pathogenesis, the objective of the present study was to investigate the prevalence of IL-6 (-174 G/C) promoter polymorphism and its association with development of MM in Brazilian individuals. We investigated the prevalence of these alleles in 52 patients and 60 healthy subjects (matched by age, sex, and race) of a Brazilian population. Thirty patients were male (42.4 percent), 24 (46.2 percent) were white and the median age at diagnosis was 58.5 years (range: 28 to 84 years). To determine the IL-6 (-174 G/C) polymorphism, molecular analysis was performed by polymerase chain reaction followed by endonuclease restriction digestion. The genotype distributions observed in the group of patients were 4 percent CC, 42 percent GC and 54 percent GG. The C allele frequency was 0.25. These results were similar to the control group, suggesting no impact of this polymorphism on the susceptibility to MM.


Assuntos
Humanos , Masculino , Feminino , Adulto , Pessoa de Meia-Idade , Idoso de 80 Anos ou mais , /genética , Mieloma Múltiplo/genética , Polimorfismo Genético , Regiões Promotoras Genéticas , Alelos , Predisposição Genética para Doença , Genótipo , Reação em Cadeia da Polimerase
16.
Braz J Med Biol Res ; 38(11): 1609-13, 2005 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-16258629

RESUMO

The biologic basis of the negative prognosis of plasmablastic myeloma is not fully understood. To determine whether histologically aggressive multiple myeloma (MM) is associated with a more angiogenic marrow environment, bone marrow samples from 50 recently diagnosed MM patients were evaluated. Twelve percent (6/50) of patients presented plasmablastic MM, and this feature correlated with moderate/strong intensity of vascular endothelial growth factor staining of plasma cells (P = 0.036). Although plasmablastic MM was not associated with increasing of microvessel density, this new evidence of increased expression of vascular endothelial growth factor on plasmablasts suggests that the adverse prognosis conferred by plasmablastic disease may be due, at least in part, to secretion of this angiogenic cytokine, also suggesting that the subset of MM patients with plasmablastic features may derive particular benefit from antiangiogenic therapies.


Assuntos
Medula Óssea/irrigação sanguínea , Mieloma Múltiplo/irrigação sanguínea , Neovascularização Patológica/patologia , Fator A de Crescimento do Endotélio Vascular/análise , Adulto , Idoso , Idoso de 80 Anos ou mais , Biomarcadores Tumorais/análise , Biópsia , Medula Óssea/patologia , Feminino , Humanos , Imuno-Histoquímica , Masculino , Microcirculação , Pessoa de Meia-Idade , Mieloma Múltiplo/metabolismo , Mieloma Múltiplo/patologia , Prognóstico
17.
Braz. j. med. biol. res ; 38(11): 1609-1613, Nov. 2005. ilus
Artigo em Inglês | LILACS | ID: lil-414729

RESUMO

The biologic basis of the negative prognosis of plasmablastic myeloma is not fully understood. To determine whether histologically aggressive multiple myeloma (MM) is associated with a more angiogenic marrow environment, bone marrow samples from 50 recently diagnosed MM patients were evaluated. Twelve percent (6/50) of patients presented plasmablastic MM, and this feature correlated with moderate/strong intensity of vascular endothelial growth factor staining of plasma cells (P = 0.036). Although plasmablastic MM was not associated with increasing of microvessel density, this new evidence of increased expression of vascular endothelial growth factor on plasmablasts suggests that the adverse prognosis conferred by plasmablastic disease may be due, at least in part, to secretion of this angiogenic cytokine, also suggesting that the subset of MM patients with plasmablastic features may derive particular benefit from antiangiogenic therapies.


Assuntos
Adulto , Idoso , Idoso de 80 Anos ou mais , Feminino , Pessoa de Meia-Idade , Humanos , Masculino , Fator A de Crescimento do Endotélio Vascular/análise , Medula Óssea/irrigação sanguínea , Mieloma Múltiplo/irrigação sanguínea , Neovascularização Patológica/patologia , Biópsia , Imuno-Histoquímica , Microcirculação , Biomarcadores Tumorais/análise , Medula Óssea/patologia , Mieloma Múltiplo/metabolismo , Mieloma Múltiplo/patologia , Prognóstico
18.
Transfus Med ; 13(5): 293-301, 2003 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-14617340

RESUMO

The efficacy of pre-operative haemodilution is limited by the reduction in haemoglobin concentration. Acellular haemoglobin-based oxygen carriers provide an alternative to colloid as a haemodiluent, potentially extending the safe limits of this procedure. The aim of this investigation was to determine whether haemodilution with a cross-linked haemoglobin solution, diaspirin cross-linked haemoglobin solution (DCLHb), would enhance the oxygen reserve compared to pentastarch. Sprague Dawley rats were placed in a metabolic box to directly measure systemic oxygen consumption (VO2). Rats were randomized to be haemodiluted to a cellular haemoglobin of 80 g L(-1) with either DCLHb or pentastarch. Oxygen reserve was assessed during isovolemic haemorrhage by determining the critical oxygen delivery (DO2crit) and haemoglobin concentration at the point of oxygen supply dependency (OSD). Following haemodilution and for the duration of the experiment, cardiac index (CI) was significantly lower and systemic vascular resistance was significantly higher in the DCLHb than the pentastarch group. The DO2crit (3.2 +/- 0.4 mL minAg(-1) and 3.4 +/- 0.5 mL minAg(-1), DCLHb versus pentastarch) and cellular haemoglobin concentration (51 +/- 9 g L(-1) and 48 +/- 9 g L(-1)), at which rats entered OSD were similar in both groups. Total haemoglobin concentration (cellular and plasma DCLHb) and arterial oxygen content were significantly higher in the DCLHb group (total haemoglobin, 66 +/- 8 g L(-1) and arterial content, 9.2 +/- 1.4 mL dL(-1)) compared to the pentastarch group (total haemoglobin, 48 +/- 9 g L(-1) and arterial content, 7.3 +/- 1.4 mL dL(-1)). Oxygen extraction ratios increased from baseline levels to 0.53 +/- 0.07 and 0.56 +/- 0.1, for the DCLHb and pentastarch groups, respectively, and were not significantly different. The increase in arterial oxygen content from DCLHb in plasma was offset by the decrease in CI observed in this group. Plasma DCLHb did not extend the limits of haemodilution beyond the capacity of the cellular haemoglobin concentration.


Assuntos
Aspirina/análogos & derivados , Aspirina/farmacologia , Perda Sanguínea Cirúrgica , Hemodiluição/métodos , Hemoglobinas/farmacologia , Oxigênio/sangue , Animais , Aspirina/uso terapêutico , Substitutos Sanguíneos/farmacologia , Substitutos Sanguíneos/uso terapêutico , Testes Hematológicos , Hemodiluição/normas , Hemoglobinas/fisiologia , Hemoglobinas/uso terapêutico , Derivados de Hidroxietil Amido/farmacologia , Derivados de Hidroxietil Amido/uso terapêutico , Modelos Animais , Consumo de Oxigênio , Cuidados Pré-Operatórios , Ratos , Ratos Sprague-Dawley
19.
Parasite Immunology ; 25(3): 135-137, mar.2003.
Artigo em Inglês | Sec. Est. Saúde SP, SESSP-IBPROD, Sec. Est. Saúde SP, SESSP-IBACERVO | ID: biblio-1065130

RESUMO

Fasciola hepatica is the causative agent of fasciolosis in many areas in America, Europe, Africa, Asia and Australia. There is an urgent need for improved methods to control the parasite's transmission. We describe the use of an experimental vaccine based on a recombinant antigen cloned from another parasite, Schistosoma mansoni (Sm14), that induces high levels of cross protection in mice against both S. mansoni and F. hepatica. Sheep and mice vaccinated with Sm14 were significantly protected against challenge infection with metacercariae of Fasciola hepatica and were completely free of the histopathological hepatic damage related to liver fluke infection. The vaccine will provide a valuable new tool to aid in transmission control of this economically important disease


Assuntos
Animais , Fasciola hepatica/imunologia , Fasciolíase/imunologia , Fasciolíase/prevenção & controle , Fígado/patologia , Schistosoma mansoni/imunologia , Ovinos , Proteínas Recombinantes/administração & dosagem , Proteínas Recombinantes/imunologia , Proteínas de Helminto/administração & dosagem , Proteínas de Helminto/imunologia , Vacinas Sintéticas/administração & dosagem , Vacinas Sintéticas/genética , Vacinação
20.
Arch Biochem Biophys ; 395(2): 199-207, 2001 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-11697857

RESUMO

In the present work, we describe the cDNA cloning, expression in Pichia pastoris, purification, and characterization of the recombinant Pisum sativum defensin 1 (rPsd1), a novel Cys-rich protein presenting four disulfide bridges and high antifungal activity. Several parameters that affect the level of protein expression were assayed. The best condition yielded 13.8 mg/L (1.50 microg/10(8) cells) of active rPsd1. The recombinant rPsd1 was purified to homogeneity by cation exchange, followed by reversed-phase HPLC, and subjected to automated amino acid sequencing, which revealed four additional amino acids (EAEA) at the N-terminal region. Circular dichroism, intrinsic fluorescence, and nuclear magnetic resonance spectroscopy analysis indicated that the recombinant protein has a very similar folding and a correct disulfide-bonding pattern when compared to native Psd1. Nevertheless, the rPsd1 presented a more species-specific antifungal activity. The importance of the N- and C-termini for Psd1 activity is pointed out.


Assuntos
Antifúngicos/farmacologia , Cisteína/química , DNA Complementar/metabolismo , Defensinas , Ervilhas/química , Pichia/metabolismo , Proteínas de Plantas/metabolismo , Sequência de Aminoácidos , Sequência de Bases , Cromatografia Líquida de Alta Pressão , Cromatografia por Troca Iônica , Dicroísmo Circular , Clonagem Molecular , Dissulfetos , Espectroscopia de Ressonância Magnética , Dados de Sequência Molecular , Plasmídeos/metabolismo , Ligação Proteica , Estrutura Terciária de Proteína , RNA Mensageiro/metabolismo , Proteínas Recombinantes/metabolismo , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Análise de Sequência de Proteína , Homologia de Sequência de Aminoácidos , Espectrometria de Fluorescência , Espectrofotometria , Fatores de Tempo
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