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1.
Photodiagnosis Photodyn Ther ; : 102622, 2021 Nov 11.
Artigo em Inglês | MEDLINE | ID: mdl-34775066

RESUMO

OBJECTIVE: To evaluate the effect of potassium iodide (KI) addition on antimicrobial photodynamic therapy (aPDT) mediated by red laser (λ = 660 nm) and methylene blue in Streptococcus mutans biofilm model. METHODS: S. mutans biofilms were cultured in 96-well plates containing BHI broth with 1% sucrose for 18 h, 10% CO2 and 37°C and divided in groups (n = 3, in triplicate): C (NaCl 0.9%); CX (0.2% chlorhexidine); P (photosensitizer); KI (10, 25 and 50 mM); PKI (10, 25 and 50 mM); L (L 1: : 100 J/cm2, 9 J; L2: 200 J/cm2, 18 J); PL (photosensitizer + L1 or L2); KIL (KI at 10, 25 and 50 mM + L1 or L2); and PKIL (photosensitizer + 10, 25 and 50 mM KI + L1 or L2). Biofilms were submitted to three pre-irradiation (PI) times (5, 10, and 15 min). After the treatments, microbial counting's reduction was analyzed by Kruskal-Wallis and post-hoc Dunn's tests, respectively, and the interaction between light parameters and the PI times by two-way ANOVA (p < 0.05). RESULTS: The S. mutans viability significantly reduced in all aPDT groups, in the presence or absence of KI (p < 0.05). For all PI times, PKIL groups (10, 25, and 50 mM) significantly differed from PL groups (p < 0.05) with a reduction of 9.0 logs reached at 50 mM of KI with 15 min of PI, irradiated at 18 J. We found no significant interaction between PI time and irradiation (p > 0.05). CONCLUSION: Addition KI to TFDA mediated by methylene blue and red laser promoted an additional effect in reducing the microbial viability of S. mutans biofilm.

2.
Int J Mol Sci ; 22(22)2021 Nov 12.
Artigo em Inglês | MEDLINE | ID: mdl-34830131

RESUMO

Production of biofuels and other value-added products from lignocellulose breakdown requires the coordinated metabolic activity of varied microorganisms. The increasing global demand for biofuels encourages the development and optimization of production strategies. Optimization in turn requires a thorough understanding of the microbial mechanisms and metabolic pathways behind the formation of each product of interest. Hydrolysis of lignocellulosic biomass is a bottleneck in its industrial use and often affects yield efficiency. The accessibility of the biomass to the microorganisms is the key to the release of sugars that are then taken up as substrates and subsequently transformed into the desired products. While the effects of different metabolic intermediates in the overall production of biofuel and other relevant products have been studied, the role of proteins and their activity under anaerobic conditions has not been widely explored. Shifts in enzyme production may inform the state of the microorganisms involved; thus, acquiring insights into the protein production and enzyme activity could be an effective resource to optimize production strategies. The application of proteomic analysis is currently a promising strategy in this area. This review deals on the aspects of enzymes and proteomics of bioprocesses of biofuels production using lignocellulosic biomass as substrate.

3.
Acta otorrinolaringol. cir. cabeza cuello ; 49(1): 57-61, 2021. ilus, tab, graf
Artigo em Espanhol | LILACS, COLNAL | ID: biblio-1152173

RESUMO

Las lesiones de la región nasofrontal en los niños son un reto diagnóstico debido a su rareza, y su potencial comunicación con el sistema nervioso central también aumenta su complicaciones. Dentro de las principales entidades de esta región se encuentran los quistes dermoides, los gliomas nasales y los encefaloceles. Un abordaje diagnóstico y terapéutico inapropiado podría generar desde simples recurrencias hasta fistulas e infecciones en el sistema nervioso central, que podrían contribuir a mayores complicaciones o incluso, poner en riesgo la vida de los pacientes.


Injuries to the naso-frontal region in children are a diagnostic challenge, associated with their rarity, their complexity also implies their potential communication with the central nervous system. Dermoid cysts, nasal gliomas, and encephaloceles are among the main entities in this region. An inappropriate diagnostic and therapeutic approach could generate from simple recurrences (as in our case), to fistulas and infections of the central nervous system that could contribute to greater complications or even put the lives of patients at risk.


Assuntos
Humanos , Masculino , Criança , Neoplasias Nasais/diagnóstico , Cisto Dermoide/diagnóstico , Nariz/anormalidades , Neoplasias Nasais/cirurgia , Cisto Dermoide/cirurgia
4.
Rev. am. med. respir ; 20(3): 273-274, sept. 2020.
Artigo em Espanhol | LILACS, BINACIS | ID: biblio-1123097

RESUMO

Paciente masculino portador de hipertesión pulmonar severa refractaria a tratamiento médico, sometido a trasplante bipulmonar durante la pandemia por SARS-CoV-2 en la ciudad de Buenos Aires. Para disminuir el riesgo de contagio se realizaron todas las medidas de prevención actualmente recomendadas, con especial énfasis en el uso del equipo de protección personal, contribuyendo de esta forma con el éxito terapéutico alcanzado.


Male patient with severe pulmonary hypertension refractory to medical treatment who underwent a double-lung transplant during the SARS-CoV-2 in the city of Buenos Aires. In order to reduce the risk of contagion all the currently recommended prevention measures were adopted, especially the use of personal protective equipment, thus contributing to the resultant therapeutic success.


Assuntos
Humanos , Transplante de Pulmão , Infecções por Coronavirus , Síndrome Respiratória Aguda Grave , Pandemias , Hipertensão Pulmonar , Pulmão
5.
Acta otorrinolaringol. cir. cabeza cuello ; 48(2): 173-178, 2020. tab, graf, ilus
Artigo em Espanhol | LILACS, COLNAL | ID: biblio-1103906

RESUMO

Actualmente no es sólito tener pacientes con síntomas causados por la implicación de las amígdalas linguales y que pasemos por alto su sospecha. Los signos y síntomas que pueden presentar los pacientes son variados, desde el síndrome de apnea obstructiva del sueño (síntoma más común) y disfagia hasta tos crónica mal abordada y, por lo mismo, tratada inadecuadamente. Presentamos los casos clínicos de dos pacientes con hipertrofia de amígdalas linguales y una revisión narrativa del tema.


Currently, it is not unusual to have patients with symptoms due to the involvement of lingual tonsils and let us go unnoticed their suspicion. The signs and symptoms that patients may present are varied, from giving obstructive sleep apnea syndrome (the most common symptom) dysphagia to poorly treated and poorly treated chronic cough. We present the clinical cases of two patients with hypertrophy of the lingual tonsils and a narrative review of the subject.


Assuntos
Humanos , Síndromes da Apneia do Sono , Diagnóstico , Hipertrofia
6.
Rev. Fac. Med. (Bogotá) ; 67(4): 623-628, Oct.-Dec. 2019. tab
Artigo em Inglês | LILACS-Express | LILACS | ID: biblio-1091988

RESUMO

Abstract Introduction: Papillary thyroid carcinoma is the most common endocrine neoplasm; therefore, markers with possible prognostic utility have been evaluated. Objective: To analyze the presence of RET/PTC1 rearrangement, lymphocytic thyroiditis and associated clinical features in patients with papillary thyroid cancer treated at the Hospital de San José in Bogotá, Colombia. Materials and methods: Clinical records of patients with complete thyroidectomy and diagnosis of papillary cancer were retrospectively identified. RNA was extracted from tumor tissue, and cDNA was obtained using inverse transcriptase to detect the rearrangement of the RET/PTC1 gene by means of qPCR. Results: 55 patients with papillary thyroid cancer were selected; 93% were females, and the mean age was 45.8 years. The most frequent histological variant was classic (49%). A relationship was found between lymphocytic thyroiditis and the number of positive nodes in segments other than central draining, as well as thyroiditis and antithyroid antibody value. No RET/PTC1 rearrangement expression was found. Conclusions: A relationship between lymphocytic thyroiditis and the number of positive nodes in segments other than central draining was found. Other molecular markers should be searched to differentiate the prognosis of these patients.


Resumen Introducción. El carcinoma papilar de tiroides es la neoplasia endocrina más común, por lo que se han evaluado marcadores con posible utilidad pronóstica. Objetivo. Analizar la presencia del rearreglo del RET/PTC1, tiroiditis linfocítica y características clínicas asociadas en pacientes con cáncer papilar de tiroides en el Hospital de San José de Bogotá D.C., Colombia. Materiales y métodos. Los casos con diagnóstico de cáncer papilar y tiroidectomía completa fueron seleccionados utilizando el registro de historias clínicas; el ARN se extrajo a partir del tejido tumoral y el ADNc se obtuvo utilizando una transcriptasa inversa para luego detectar el rearreglo del gen RET/PTC1 por medio de qPCR. Resultados. Se seleccionaron 55 pacientes con cáncer papilar de tiroides; 93% correspondió a género femenino, la edad promedio fue de 45.8 años y la variante histológica más frecuente fue la clásica (49%). Se evidenció una relación entre tiroiditis linfocítica y la cantidad de ganglios positivos en segmentos distintos al vaciamiento central, así como la tiroiditis y el valor de los anticuerpos antitiroideos. No se identificó la expresión del rearreglo RET/PTC1 en las muestras analizadas. Conclusiones. Se muestra una relación entre tiroiditis linfocítica y la cantidad de ganglios positivos en segmentos distintos al vaciamiento central. Se debe continuar la búsqueda de otros marcadores moleculares que permitan diferenciar el pronóstico en estos pacientes.

7.
Front Microbiol ; 10: 3055, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-32010106

RESUMO

Bacteria have evolved complex sensing and signaling systems to react to their changing environments, most of which are present in all domains of life. Canonical bacterial sensing and signaling modules, such as membrane-bound ligand-binding receptors and kinases, are very well described. However, there are distinct sensing mechanisms in bacteria that are less studied. For instance, the sensing of internal or external cues can also be mediated by changes in protein conformation, which can either be implicated in enzymatic reactions, transport channel formation or other important cellular functions. These activities can then feed into pathways of characterized kinases, which translocate the information to the DNA or other response units. This type of bacterial sensory activity has previously been termed protein activity sensing. In this review, we highlight the recent findings about this non-canonical sensory mechanism, as well as its involvement in metabolic functions and bacterial motility. Additionally, we explore some of the specific proteins and protein-protein interactions that mediate protein activity sensing and their downstream effects. The complex sensory activities covered in this review are important for bacterial navigation and gene regulation in their dynamic environment, be it host-associated, in microbial communities or free-living.

8.
Funct Integr Genomics ; 19(1): 171-190, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30244303

RESUMO

Elevated CO2 along with drought is a serious global threat to crop productivity. Therefore, understanding the molecular mechanisms plants use to protect these stresses is the key for plant growth and development. In this study, we mimicked natural stress conditions under a controlled Soil-Plant-Atmosphere-Research (SPAR) system and provided the evidence for how miRNAs regulate target genes under elevated CO2 and drought conditions. Significant physiological and biomass data supported the effective utilization of source-sink (leaf to root) under elevated CO2. Additionally, elevated CO2 partially rescued the effect of drought on total biomass. We identified both known and novel miRNAs differentially expressed during drought, CO2, and combined stress, along with putative targets. A total of 32 conserved miRNAs belonged to 23 miRNA families, and 25 novel miRNAs were identified by deep sequencing. Using the existing sweet potato genome database and stringent analyses, a total of 42 and 22 potential target genes were predicted for the conserved and novel miRNAs, respectively. These target genes are involved in drought response, hormone signaling, photosynthesis, carbon fixation, sucrose and starch metabolism, etc. Gene ontology and KEGG ontology functional enrichment revealed that these miRNAs might target transcription factors (MYB, TCP, NAC), hormone signaling regulators (ARF, AP2/ERF), cold and drought factors (corA), carbon metabolism (ATP synthase, fructose-1,6-bisphosphate), and photosynthesis (photosystem I and II complex units). Our study is the first report identifying targets of miRNAs under elevated CO2 levels and could support the molecular mechanisms under elevated CO2 in sweet potato and other crops in the future.


Assuntos
Dióxido de Carbono/metabolismo , Regulação da Expressão Gênica de Plantas , Genoma de Planta , Ipomoea batatas/genética , MicroRNAs/genética , Folhas de Planta/genética , Raízes de Plantas/genética , Biomassa , Ciclo do Carbono/genética , Secas , Regulação da Expressão Gênica no Desenvolvimento , Ontologia Genética , Sequenciamento de Nucleotídeos em Larga Escala , Ipomoea batatas/crescimento & desenvolvimento , Ipomoea batatas/metabolismo , MicroRNAs/metabolismo , Anotação de Sequência Molecular , Fotossíntese/genética , Folhas de Planta/crescimento & desenvolvimento , Folhas de Planta/metabolismo , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Raízes de Plantas/crescimento & desenvolvimento , Raízes de Plantas/metabolismo , Transdução de Sinais , Estresse Fisiológico/genética
9.
Proc Natl Acad Sci U S A ; 115(52): 13365-13370, 2018 12 26.
Artigo em Inglês | MEDLINE | ID: mdl-30541885

RESUMO

The chemoreceptor array, a remarkably ordered supramolecular complex, is composed of hexagonally packed trimers of receptor dimers networked by a histidine kinase and one or more coupling proteins. Even though the receptor packing is universal among chemotactic bacteria and archaea, the array architecture has been extensively studied only in selected model organisms. Here, we show that even in the complete absence of the kinase, the cluster II arrays in Vibrio cholerae retain their native spatial localization and the iconic hexagonal packing of the receptors with 12-nm spacing. Our results demonstrate that the chemotaxis array is versatile in composition, a property that allows auxiliary chemotaxis proteins such as ParP and CheV to integrate directly into the assembly. Along with its compositional variability, cluster II arrays exhibit a low degree of structural stability compared with the ultrastable arrays in Escherichia coli We propose that the variability in chemoreceptor arrays is an important mechanism that enables the incorporation of chemotaxis proteins based on their availability.


Assuntos
Células Quimiorreceptoras/metabolismo , Vibrio cholerae/metabolismo , Vibrio cholerae/fisiologia , Archaea/metabolismo , Bactérias/metabolismo , Proteínas de Bactérias/metabolismo , Quimiotaxia , Escherichia coli/metabolismo , Proteínas de Escherichia coli/metabolismo , Variação Genética/genética , Proteínas de Membrana/metabolismo , Proteínas Quimiotáticas Aceptoras de Metil/metabolismo , Ligação Proteica , Transdução de Sinais
10.
J Bacteriol ; 200(15): e00793-17, 2018 03 12.
Artigo em Inglês | MEDLINE | ID: mdl-29531180

RESUMO

Most motile bacteria are able to bias their movement towards more favorable environments or to escape from obnoxious substances by a process called chemotaxis. Chemotaxis depends on a chemosensory system that is able to sense specific environmental signals and generate a behavioral response. Typically, the signal is transmitted to the bacterial flagellum, ultimately regulating the swimming behavior of individual cells. Chemotaxis is mediated by proteins that assemble into large, highly ordered arrays. It is imperative for successful chemotactic behavior and cellular competitiveness that chemosensory arrays form and localize properly within the cell. Here we review how chemotaxis arrays form and localize in Vibrio cholerae and Vibrio parahaemolyticus We focus on how the ParC/ParP-system mediates cell cycle-dependent polar localization of chemotaxis arrays and thus ensures proper cell pole development and array inheritance upon cell division.

11.
Repert. med. cir ; 27(1): 30-35, 2018.
Artigo em Inglês, Espanhol | LILACS, COLNAL | ID: biblio-911243

RESUMO

Introducción: la prevalencia de la osteoporosis aumenta con la edad, así como la morbimortalidad secundaria a fracturas por fragilidad impactando los costos en salud. Las estrategias creadas han mejorado la detección y manejo de las fracturas. Objetivo: describir las características clínicas de los pacientes con fracturas por fragilidad y su manejo en el Hospital de San José de Bogotá de febrero 2015 a julio 2016, luego de establecer un programa de identificación y prevención de fracturas. Métodos: estudio descriptivo de corte transversal. Se evaluaron las historias clínicas con diagnóstico de osteoporosis severa, analizando las características demográficas, clínicas y tratamiento recibido. Resultados: de 621 pacientes con diagnóstico de osteoporosis y fracturas asociadas, 184 cumplieron los criterios de inclusión. La edad media fue de 71.5 años (DE: 11,6). Las localizaciones más frecuentes fueron radio distal 36%, vertebrales 34%, fémur proximal 18% y otras 11%. El 30% tenían diagnóstico de osteoporosis, de ellos 30% recibieron tratamiento completo antes de la fractura y 57,6% posterior. Conclusión: las fracturas por fragilidad son frecuentes en osteoporosis, aunque hay subdiagnóstico y subtratamiento. La prevención de la segunda fractura se refleja en el aumento del número de pacientes que recibieron tratamiento adecuado, demostrando la necesidad de crear programas de educación.


Introduction: the prevalence of osteoporosis increases with aging, as well as, morbidity and mortality secondary to fragility fractures, impacting costs of healthcare. New strategies have enhanced fragility fractures detection and management. Objective: to describe the clinical features of patients with fragility fractures and their management at Hospital de San José, Bogotá, from February 2015 to July 2016, after establishing a fracture identification and prevention program. Methods: a cross sectional descriptive study. Clinical records of patients diagnosed with severe osteoporosis were reviewed. Demographic and clinical characteristics and treatment outcomes were analyzed. Results: out of 621 patients with osteoporosis-related fractures, 184 met the inclusion criteria. Mean age was 71.5 years (SD: 11.6). Fractures affected the distal radius in 36%, vertebrae in 34%, proximal femur in 18% and other sites in 11%. Osteoporosis was diagnosed in 30% of patients of which 30% received complete treatment for osteoporosis prior to fracture and 57.6% after fracture. Conclusions: fragility fractures are usually associated with osteoporosis, although there is underdiagnosis and under-treatment. Prevention of a future fracture is reflected in the increasing number of patients who received appropriate treatment, demonstrating the need to create education programs.


Assuntos
Humanos , Fraturas Ósseas , Fraturas por Osteoporose , Osso e Ossos , Osteoporose , Deficiência de Vitamina D
12.
Elife ; 62017 10 23.
Artigo em Inglês | MEDLINE | ID: mdl-29058677

RESUMO

Chemotaxis proteins organize into large, highly ordered, chemotactic signaling arrays, which in Vibrio species are found at the cell pole. Proper localization of signaling arrays is mediated by ParP, which tethers arrays to a cell pole anchor, ParC. Here we show that ParP's C-terminus integrates into the core-unit of signaling arrays through interactions with MCP-proteins and CheA. Its intercalation within core-units stimulates array formation, whereas its N-terminal interaction domain enables polar recruitment of arrays and facilitates its own polar localization. Linkage of these domains within ParP couples array formation and localization and results in controlled array positioning at the cell pole. Notably, ParP's integration into arrays modifies its own and ParC's subcellular localization dynamics, promoting their polar retention. ParP serves as a critical nexus that regulates the localization dynamics of its network constituents and drives the localized assembly and stability of the chemotactic machinery, resulting in proper cell pole development.


Assuntos
Proteínas de Bactérias/metabolismo , Quimiotaxia , Proteínas de Membrana/metabolismo , Multimerização Proteica , Vibrio cholerae/fisiologia , Ligação Proteica , Transporte Proteico
13.
J Vis Exp ; (123)2017 05 15.
Artigo em Inglês | MEDLINE | ID: mdl-28570527

RESUMO

The ability to study the intracellular localization of proteins is essential for the understanding of many cellular processes. In turn, this requires the ability to obtain single cells for fluorescence microscopy, which can be particularly challenging when imaging cells that exist within bacterial communities. For example, the human pathogen Vibrio parahaemolyticus exists as short rod-shaped swimmer cells in liquid conditions that upon surface contact differentiate into a subpopulation of highly elongated swarmer cells specialized for growth on solid surfaces. This paper presents a method to perform single cell fluorescence microscopy analysis of V. parahaemolyticus in its two differential states. This protocol very reproducibly induces differentiation of V. parahaemolyticus into a swarmer cell life-cycle and facilitates their proliferation over solid surfaces. The method produces flares of differentiated swarmer cells extending from the edge of the swarm-colony. Notably, at the very tip of the swarm-flares, swarmer cells exist in a single layer of cells, which allows for their easy transfer to a microscope slide and subsequent fluorescence microscopy imaging of single cells. Additionally, the workflow of image analysis for demographic representation of bacterial societies is presented. As a proof of principle, the analysis of the intracellular localization of chemotaxis signaling arrays in swimmer and swarmer cells of V. parahaemolyticus is described.


Assuntos
Microscopia de Fluorescência/métodos , Análise de Célula Única/métodos , Vibrio parahaemolyticus/citologia , Diferenciação Celular , Quimiotaxia , Transdução de Sinais , Vibrio parahaemolyticus/fisiologia
14.
Front Plant Sci ; 7: 1437, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27713759

RESUMO

Melon (Cucumis melo L.) is a phenotypically diverse eudicot diploid (2n = 2x = 24) has climacteric and non-climacteric morphotypes and show wide variation for fruit firmness, an important trait for transportation and shelf life. We generated 13,789 SNP markers using genotyping-by-sequencing (GBS) and anchored them to chromosomes to understand genome-wide fixation indices (Fst) between various melon morphotypes and genomewide linkage disequilibrium (LD) decay. The FST between accessions of cantalupensis and inodorus was 0.23. The FST between cantalupensis and various agrestis accessions was in a range of 0.19-0.53 and between inodorus and agrestis accessions was in a range of 0.21-0.59 indicating sporadic to wide ranging introgression. The EM (Expectation Maximization) algorithm was used for estimation of 1436 haplotypes. Average genome-wide LD decay for the melon genome was noted to be 9.27 Kb. In the current research, we focused on the genome-wide divergence underlying diverse melon horticultural groups. A high-resolution genetic map with 7153 loci was constructed. Genome-wide segregation distortion and recombination rate across various chromosomes were characterized. Melon has climacteric and non-climacteric morphotypes and wide variation for fruit firmness, a very important trait for transportation and shelf life. Various levels of QTLs were identified with high to moderate stringency and linked to fruit firmness using both genome-wide association study (GWAS) and biparental mapping. Gene annotation revealed some of the SNPs are located in ß-D-xylosidase, glyoxysomal malate synthase, chloroplastic anthranilate phosphoribosyltransferase, and histidine kinase, the genes that were previously characterized for fruit ripening and softening in other crops.

15.
Front Microbiol ; 5: 597, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-25429286

RESUMO

Anaerobic digestion (AD) is a biological process where different trophic groups of microorganisms break down biodegradable organic materials in the absence of oxygen. A wide range of AD technologies is being used to convert livestock manure, municipal and industrial wastewaters, and solid organic wastes into biogas. AD gains importance not only because of its relevance in waste treatment but also because of the recovery of carbon in the form of methane, which is a renewable energy and is used to generate electricity and heat. Despite the advances on the engineering and design of new bioreactors for AD, the microbiology component always poses challenges. Microbiology of AD processes is complicated as the efficiency of the process depends on the interactions of various trophic groups involved. Due to the complex interdependence of microbial activities for the functionality of the anaerobic bioreactors, the genetic expression of mcrA, which encodes a key enzyme in methane formation, is proposed as a parameter to monitor the process performance in real time. This review evaluates the current knowledge on microbial groups, their interactions, and their relationship to the performance of anaerobic biodigesters with a focus on using mcrA gene expression as a tool to monitor the process.

16.
Biomedica ; 34 Suppl 1: 16-22, 2014 Apr.
Artigo em Espanhol | MEDLINE | ID: mdl-24968032

RESUMO

INTRODUCTION: Extended-spectrum beta-lactamases (ESBL) are an emerging resistance phenomenon with particular incidence in Latin America. In Colombia there is very little information regarding the risk factors associated with its acquisition. OBJECTIVE: To determine the risk factors that are associated with infection or colonization by Escherichia coli or Klebsiella pneumoniae producing ESBL in patients older than 18 years. MATERIALS AND METHODS: A case-control study, ratio 1:1, in patients with an isolate from any sample of E. coli or K. pneumoniae producing ESBL in the period from January 2009 to November 2011 at San José University Hospital in Bogotá (Colombia). RESULTS: We studied 110 cases and 110 controls, 62.7% were E. coli and 37.3% K. pneumoniae . In the multivariate analysis the independent risk factors found were: chronic renal failure (odds ratio [OR] 2.99, confidence interval 95% [95% CI] 1.10-8.11, p=0.031), urologic surgery (OR 4.78 95% CI 1.35 to 16.87, p=0.015), history of antibiotic use in the previous three months (OR 2.24, 95% CI 1.09 ­ 4.60, p=0.028), nosocomial origin of infection (OR=2.92 95% CI 1.39 ­ 6.13, p=0.004) and previous hospitalization (OR 1,59, 95% CI=1.03 ­ 2.46, p=0,036). CONCLUSION: Anticipating the resistance pattern of the organism infecting a patient based on risk factors may allow the choice of appropriate empirical antibiotic therapy, which will have an impact on reducing patients' morbidity and mortality.


Assuntos
Antibacterianos/farmacologia , Farmacorresistência Bacteriana Múltipla , Infecções por Escherichia coli/microbiologia , Escherichia coli/enzimologia , Infecções por Klebsiella/microbiologia , Klebsiella pneumoniae/enzimologia , beta-Lactamas/farmacologia , Adulto , Idoso , Antibacterianos/metabolismo , Estudos de Casos e Controles , Colômbia , Infecção Hospitalar/epidemiologia , Infecção Hospitalar/microbiologia , Farmacorresistência Bacteriana Múltipla/genética , Escherichia coli/efeitos dos fármacos , Escherichia coli/genética , Escherichia coli/isolamento & purificação , Infecções por Escherichia coli/epidemiologia , Feminino , Humanos , Hospedeiro Imunocomprometido , Infecções por Klebsiella/epidemiologia , Klebsiella pneumoniae/efeitos dos fármacos , Klebsiella pneumoniae/genética , Klebsiella pneumoniae/isolamento & purificação , Masculino , Pessoa de Meia-Idade , Complicações Pós-Operatórias/microbiologia , Estudos Retrospectivos , Fatores de Risco , Centros de Atenção Terciária , Resistência beta-Lactâmica/genética , beta-Lactamas/metabolismo
17.
Biomédica (Bogotá) ; 34(supl.1): 16-22, abr. 2014. tab
Artigo em Espanhol | LILACS | ID: lil-712417

RESUMO

Introducción. Las betalactamasas de espectro extendido (BLEE) son un fenómeno de resistencia emergente de particular incidencia en América Latina. En Colombia existe poca información sobre los factores de riesgo asociados con su adquisición. Objetivo. Determinar los factores de riesgo que están asociados a la infección o colonización por Escherichia coli o Klebsiella pneumoniae productoras de BLEE en pacientes mayores de 18 años. Materiales y métodos. Se llevó a cabo un estudio de casos y controles con relación 1:1 en pacientes con aislamientos de E. coli o K. pneumoniae productoras de BLEE en cualquier tipo de muestra durante el periodo de enero de 2009 a noviembre de 2011 en el Hospital Universitario de San José. Resultados. Se estudiaron 110 casos y 110 controles; 62,7 % correspondió a E. coli y 37,3 %, a K. pneumoniae . Como factores de riesgo independiente en el análisis multivariado se encontraron la insuficiencia renal crónica (OR=2,99; IC 95%, 1,10-8,11; p=0,031), la cirugía urológica (OR=4,78; IC 95%, 1,35-16,87; p=0,015), el antecedente de uso de antibióticos en los tres meses anteriores (OR=2,24; IC 95%, 1,09-4,60; p=0,028), el origen hospitalario de la infección (OR=2,92; IC 95%, 1,39-6,13; p=0,004) y la hospitalización previa (OR=1,59; IC 95%, 1,03-2,46; p=0,036). Conclusión. Anticiparse al patrón de resistencia del microorganismo que infecta a un paciente con base en los factores de riesgo asociados permitiría la elección de un tratamiento antibiótico empírico apropiado, con el fin de lograr la disminución de la morbimortalidad de los pacientes.


.


Assuntos
Adulto , Idoso , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Antibacterianos/farmacologia , Farmacorresistência Bacteriana Múltipla , Infecções por Escherichia coli/microbiologia , Escherichia coli/enzimologia , Infecções por Klebsiella/microbiologia , Klebsiella pneumoniae/enzimologia , beta-Lactamas/farmacologia , Antibacterianos/metabolismo , Estudos de Casos e Controles , Colômbia , Infecção Hospitalar/epidemiologia , Infecção Hospitalar/microbiologia , Farmacorresistência Bacteriana Múltipla/genética , Infecções por Escherichia coli/epidemiologia , Escherichia coli/efeitos dos fármacos , Escherichia coli/genética , Escherichia coli/isolamento & purificação , Hospedeiro Imunocomprometido , Infecções por Klebsiella/epidemiologia , Klebsiella pneumoniae/efeitos dos fármacos , Klebsiella pneumoniae/genética , Klebsiella pneumoniae/isolamento & purificação , Complicações Pós-Operatórias/microbiologia , Estudos Retrospectivos , Fatores de Risco , Centros de Atenção Terciária , Resistência beta-Lactâmica/genética , beta-Lactamas/metabolismo
18.
Acta otorrinolaringol. cir. cabeza cuello ; 40(2): 143-147, abr.-jun. 2012. ilus
Artigo em Espanhol | LILACS | ID: lil-682825

RESUMO

Los tumores malignos en el paladar óseo son una patología poco frecuente. Se reportan del 1 al 5% de las neoplasias malignas de la cavidad oral, donde dos tercios de las lesiones son carcinomas escamocelulares. Por lo general se presentan como una masa o ulceración no dolorosa en el techo de la boca o el borde alveolar. La invasión ósea ocurre tardíamente y las lesiones se pueden tornar exofíticas cuando no se tratan. Uno de los factores predisponentes para esta patología es el tabaquismo invertido. Las metástasis clínicas se manifiestan entre un 10 a 25%. En estadios tempranos puede ser tratado de manera efectiva con cirugía o radioterapia. Presentamos el caso de un paciente con una lesión ulcerada en paladar óseo a nivel del torus palatino, de tres meses de evolución, con antecedente de alcoholismo y tabaquismo. Se documentó carcinoma escamocelular y se le practicó palatectomía parcial, con colocación de prótesis dental en paladar óseo …


Malignant tumors of the palate are an infrequent pathology. It accounts about 1 to 5% of malignancies of the oral cavity, where two thirds of lesions are squamous cell carcinomas. Usually palate tumors appear with a painless mass or an ulceration on the roof of the mouth or alveolar ridge. Bone invasion occurs late. The lesions can become exophytic when left untreated. One of the predisposing factors for this pathology is the Reverse Smoking. Clinical metastases are found between 10 to 25%. In early stages, these tumors can be succesfully treated with surgery or radiation therapy. We report a patient with and ulcerated lesion in hard palate at palatine torus level with 3 months course. He has alcoholism and smoking history. It was documented a squamous cells carcinoma. It was performed a partial palatectomy with placement of dental prostheses …


Assuntos
Humanos , Carcinoma , Palato , Palato/cirurgia
19.
Cienc. enferm ; 15(1): 61-74, abr. 2009. tab
Artigo em Espanhol | LILACS | ID: lil-556241

RESUMO

Estudio de investigación acción participativa que tuvo como objetivo desarrollar y evaluar, junto con la comunidad, la percepción del programa fundamentado en un modelo socioecológico, con el fin de promover la inclusión social de las personas en situación de discapacidad y sus familias residentes en una comunidad del municipio de Chía en Colombia, desde el desarrollo personal, familiar y comunitario. Inicialmente se identificaron los significados que alrededor de la discapacidad se han construido, los cuales están relacionados con los problemas que deben afrontar en su cotidianidad y como una condición que les permite reconocer su valía y sus potencialidades. En el proceso de construcción de este modelo participaron 14 personas en situación de discapacidad, sus cuidadores, 8 líderes comunitarios, 6 miembros de la Junta de Acción Comunal y un equipo conformado por profesionales de enfermería, educación y psicología. La identificación de los recursos disponibles se llevó a cabo a través de foros comunitarios y el uso de estrategias como las metáforas del árbol y del sol. Los resultados muestran que el desarrollo personal y familiar, se logra desde la percepción de bienestar y respaldo; el desarrollo comunitario desde el apoyo social, la satisfacción de necesidades, la identificación y la generación de recursos.


The purpose of this participative action research was to develop and evaluate with the community, the perception of a program based on a socio ecological model for the social inclusion of people with disabilities and their families who live in a community of the municipality of Chia, in Colombia. The project was developed in three phases. During the planning phase, the researchers identified the meanings of "disability condition" that are given by the patients, their families and the support networks and it was found that these were related to the difficulties disabled people and their families must face in their daily living and how this condition might allow them to recognize their sheer value and potential. During the second phase of action and the third phase of evaluation, 14 people with disabilities, their caregivers, 14 community leaders and a team formed by nursing, psychology and education professionals were involved in the construction and development of the model. The identification of available resources was carried out through interviews, focal groups and community forums using strategies such as the tree and the sun metaphors. The results show that the personal and family development nucleus is accomplished from the perception of welfare and support. The community development nucleus is accomplished from the social support, the satisfaction of needs, and the identification of resources and resource generation.


Assuntos
Humanos , Pessoas com Deficiência , Participação da Comunidade , Apoio Social , Serviços de Reabilitação
20.
Univ. odontol ; 27(59): 6-14, ene.-dec. 2008. ilus, tab
Artigo em Espanhol | LILACS | ID: lil-587049

RESUMO

PROPÓSITO: Estudiar las posibles alteraciones de las propiedades mecánicas de aleaciones de cobalto-cromo (módulo de Young), al comparar materiales reciclados y procedimientos de colado, para establecer si estas aleaciones se pueden reutilizar en la elaboración de estructuras para prótesis parcial removible. MÉTODOS: Para este estudio experimental in vitro se tomaron 60 especímenes colados en aleación de Co-Cr en forma de probeta, los cuales se dividieron en 2 grupos, según el tipo de aleación, Wironit L.A® de Bego y Remanium® 800 de Dentaurum. Cada grupo se dividió en 6 subgrupos de 5 especímenes cada uno según el estado del material y la centrífuga empleada en su elaboración: material nuevo en centrífuga por inducción, material 50% nuevo-50% reutilizado en centrífuga por inducción, material reutilizado totalmente en centrífuga por inducción, material nuevo en centrífuga convencional, material 50% nuevo-50% reutilizado en centrífuga convencional, y material reutilizado totalmente en centrífuga convencional. RESULTADOS: El estado del material es el factor que más ocasionó variabilidad en el módulo de Young, siendo el 50% nuevo-50% reutilizado significativamente mayor que los otros dos estados del material, mientras que al comparar el estado nuevo y reutilizado los valores son similares. El módulo de Young se vio menos afectado por el tipo de centrífuga que se utilice en el proceso de colado. El tipo de aleación de cobalto-cromo Wironit® y Remanium® no estuvo asociado de manera significativa a la variación del módulo de Young, a pesar de presentar ciertas diferencias en los porcentajes de los elementos constituyentes de las aleaciones (a=005).


PURPOSE: Analyze the possible alterations of mechanical properties of cobalt-chromium alloys measured through the Young’s module, when comaring new and/or recycled materials and casting techniques, in order to establish if those alloys can be resued to fabricate removable partial dentures. METHODS: For this experimental in vitro study, 60 specimens casted in Co-Cr alloys were assigned to two groups according to the type of alloy, Bego’s Wironit L.A® and Dentaurum’s Remanium® 800. The two groups were divided into 6 subgroups of 5 specimens each regarding state of the material and type of centrifuge used: new material with induction centrifuge,50%-new-and-50%-recycled material with induction centrifuge, recycled material with induction centrifuge, new material with conventional centrifuge, 50%-new-and-50%-recycled material with conventional centrifuge, and recycled material with conventional centrifuge. FINDINGS: The state of the material is the factor that was more associated to the variation of the Young’s module, being the 50%-newand- 50%-recylced material that showed the highest variation. When comparing new and used materials, values were similar. Young’s module was less affected by the type of centrifuge used. There was not a significant association between the kind of Co-Cr alloy,Wironit® y Remanium®, and the variation in the Young’s module, in spite of presenting variations in composition (á=005).


Assuntos
Elasticidade , Prótese Periodontal , Prostodontia
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