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1.
Acta Biomed ; 93(3)2022 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-35775781

RESUMO

BACKGROUND AND AIM: DNA repair systems are functionally essential for the maintenance of life and among these, we can highlight the MutS system, subdivided into MutSα (hMSH2 and hMSH6) and MutSß (hMSH2 and hMSH3). The objective of this study was to analyze the expression of hMSH2 and hMSH6 repair genes in radiology technicians exposed to low radiation doses. METHODS: Thirty workers occupationally exposed to ionizing radiation and twenty-five non-exposed were included in this study. Gene expression was analyzed by qPCR. Peripheral blood samples were collected from both groups for total RNA isolation. RESULTS: It was observed a five-fold increase (p=0.006) in the hMSH2 repair gene expression in those exposed to radiation and a weak but significant correlation (p=0.041) with the hMSH6 genes when we associated the number of hours of exposure with gene expression. CONCLUSIONS: The longer the exposure time, the greater the activation of this component of the repair system. APPLICATION TO PRACTICE: Blood count parameters could did not alter with radiation exposure. X-rays used by radiology technicians in imaging tests can damage the DNA to the point of activating the MutS repair system and that there is a greater tendency of expression of this system in professionals that had undergone longer exposure.

2.
Curr Drug Saf ; 2022 Jun 20.
Artigo em Inglês | MEDLINE | ID: mdl-35726430

RESUMO

INTRODUCTION: Brazil has been facing the pandemic of COVID-19 since march 2020. To date, more than 540,000 people have died from this disease in the country. There are estimates that indicate that the population exposed to SARS-CoV-2 represents 1 to 20%, however, these data are questionable due to the number of asymptomatic and untested individuals. As a result, vaccination for COVID-19 has become the main means of achieving herd immunity. OBJECTIVES: To demonstrate, through local sampling, that broad and rapid vaccination may decrease the rate of COVID-19 detection in individuals potentially exposed to the SARS-CoV-2 virus. RESULTS: A total of 1,128 individuals were studied, including students and health professionals from Centro Universitário FMABC, who received the two doses of the vaccine for COVID-19 (Oxford/Astrazeneca ® and CoronaVac®). There was a 41% reduction in the demand for RT-PCR test after vaccination, in the studied period. And a 78.3% reduction in positive results after vaccination started Conclusion: The results of this study showed that, even vaccinating a population with higher exposure to the risk of contamination, there was a significant reduction in test positivity and in the demand to perform these tests. Emphasizing that vaccination is the best strategy to achieve herd immunity and to reduce the spread of the disease.

3.
Int J Exp Pathol ; 103(3): 112-120, 2022 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-35569033

RESUMO

The creation of multigene panels for prognostic and predictive purposes allows a more accurate indication of adjuvant chemotherapy for patients with breast cancer. In a previous study, we reproduced a multigene panel of 21 genes based on the commercial Oncotype-DX method. We submitted 183 embedded specimens obtained from breast surgery on patients with locoregional disease (stages I to III) between 2005 and 2010 performed at the Hospitals of the Medical School of the ABC Foundation. When we analysed the correlations between the score of the multigene panel and the progression-free interval (PFI) in all patients, we did not find a statistically significant association. However, when we selected only the 71 samples that had amplification of at least eight non-housekeeping genes, we observed that those with scores above the 75th percentile had a significantly lower PFI (p = .0054). Samples processed with nonbuffered formaldehyde were associated with a worse quality of extracted RNA (p = .004) and a significantly higher multigene panel score (p = .021). We conclude that variations in the pre-analytical processing of specimens destined for multigene panel amplification can significantly affect the results, with a potential impact on clinical management.


Assuntos
Neoplasias da Mama , Recidiva Local de Neoplasia , Biópsia , Neoplasias da Mama/patologia , Quimioterapia Adjuvante , Feminino , Perfilação da Expressão Gênica/métodos , Humanos , Recidiva Local de Neoplasia/tratamento farmacológico , Recidiva Local de Neoplasia/genética , Recidiva Local de Neoplasia/patologia , Prognóstico
4.
ABCS health sci ; 47: e022207, 06 abr. 2022. ilus, tab
Artigo em Inglês | LILACS | ID: biblio-1372363

RESUMO

INTRODUCTION: Several studies have shown that early mobilization is safe and beneficial for patients admitted to the intensive care units (ICUs), especially for those with mechanical ventilation (MV). OBJECTIVE: To investigate the benefits of early mobilization physiotherapeutic techniques applied to patients who suffered craniocerebral trauma (CCT). METHODS: This is an experimental study that evaluated clinical data from 27 patients. In sedated patients, mobilization and passive stretching were performed on the upper and lower limbs; in those without sedation, active-assisted, free and resisted exercises were included. RESULTS: The experimental group was composed of 51.8% of the participants and the control group by 48.2%, the majority being male (81.5%) with a median age of 43 years. The patients in the experimental group had an average of 9.5 days (2.2-14.7) of mechanical ventilation (MV), and those belonging to the control group, of 17 days (7-21.7) with MV (p=0.154). The patients in the experimental group had an average of 13.5 days in the ICU, against an average of 17 days in the control group (p=0.331), and an average of 20.5 days in hospital against 24 days in the control group (p=0.356). CONCLUSION: Early mobilization should be applied to critically ill patients as it can decrease the length of stay in the ICU and the hospital.


INTRODUÇÃO: Diversos estudos têm mostrado que a mobilização precoce é segura e benéfica para pacientes internados em unidades de terapia intensiva (UTIs), especialmente para aqueles com ventilação mecânica (VM). OBJETIVO: Investigar os benefícios das técnicas fisioterapêuticas de mobilização precoce aplicada aos pacientes que sofreram traumatismo cranioencefálico (TCE). MÉTODOS: Trata-se de um estudo quasi-randomizado, que incluiu 27 com TCE divididos em dois grupos: controle (n=13) e experimental (n=14) pacientes. No grupo experimental, os pacientes sedados foram submetidos à mobilização e alongamentos passivos nos membros superiores e inferiores; naqueles sem sedação, foram incluídos exercícios ativo-assistidos, livres e resistidos. RESULTADOS: O grupo experimental foi composto por 51,8% dos participantes da pesquisa e o grupo controle por 48,2%, sendo a maioria do sexo masculino (81,5%) com mediana de idade de 43 anos. Os pacientes do grupo experimental apresentaram média de 9,5 dias (2,2-14,7) de ventilação mecânica, e os pertencentes ao grupo controle, de 17 dias (7-21,7) com de VM (p=0,154). Os pacientes do grupo experimental apresentaram média de 13,5 dias de internação em UTI, contra média de 17 dias do grupo controle (p=0,331), e média de 20,5 dias de internação hospitalar contra 24 dias do grupo controle (p=0,356). CONCLUSÃO: A mobilização precoce é uma técnica que deve ser aplicada em pacientes críticos dentro das UTIs, pois pode diminuir o tempo de internação na UTI e hospitalar.


Assuntos
Humanos , Masculino , Feminino , Serviço Hospitalar de Fisioterapia , Deambulação Precoce , Lesões Encefálicas Traumáticas/terapia , Unidades de Terapia Intensiva , Hospitais Estaduais
6.
Rev. SOBECC (Online) ; 27: 1-7, 01-01-2022.
Artigo em Português | LILACS, BDENF - Enfermagem | ID: biblio-1377522

RESUMO

Objetivo: Relatar a experiência de um júri simulado como estratégia didático-pedagógica no processo de ensino-aprendizagem do componente curricular Centro de Material e Esterilização ­ vinculado ao curso de Enfermagem da Faculdade de Enfermagem Nossa Senhora das Graças (FENSG), da Universidade de Pernambuco ­ utilizando uma abordagem remota, dadas as circunstâncias pandêmicas. Método: Relato de experiência elaborado por dis-centes e docentes da Universidade de Pernambuco. A atividade consistiu em um júri simulado, fundamentado em matéria jornalística sobre o caso Porter Adventist Hospital, relativo às consequências de supostas práticas inadequadas no processamento de produtos para saúde. O conteúdo foi traduzido e disponi-bilizado aos discentes, que atuaram como advogados de acusação e de defesa no caso, conforme determinação dos docentes. Três etapas compuseram o júri simulado: acusação, réplica e tréplica, com tempo estipulado de 15 minutos para acusação e réplica, e metade do tempo inicial para tréplica em resposta à defesa. Resultados: A acusação sustentou suas alegações, majoritariamente, na Resolução da Diretoria Colegiada da Agência Nacional de Vigilância Sanitária no 15/2012. Em contrapartida, a defesa ratificou seus posicionamentos na insuficiência de atestatórios contra o hospital Porter, réu do caso. Conclusão: No júri simulado, a formulação de argumentos impacta o processo de ensino-aprendizagem ao qualificar futuros enfermeiros para os requisitos de boas práticas no processamento de produtos para saúde, além da operacionalização, supervisão e gerenciamento dentro do Centro de Material e Esterilização.


bjective: To report the experience of a simulated jury as a didactic-pedagogical strategy in the teaching-learning process of the curricular component: Material and Sterilization Center­linked to the Nursing course at Nossa Senhora das Graças Nursing School (FENSG), Universidade de Pernambuco­using a remote approach, due to the pandemic circumstances. Method: Experience report prepared by students and professors at Universidade de Pernambuco. The activity consisted of a mock jury, based on journalistic material about the Porter Adventist Hospital case, concerning the consequen-ces of alleged inappropriate practices in the processing of health products. The content was translated and made available to the students, who acted as prosecution and defense attorneys in the case, as determined by the professors. Three stages made up the mock jury: indictment, rebuttal and rejoinder, with a stipulated time of 15 minutes for the indictment and rebuttal, and half the initial time for the rejoinder in response to the defense. Results: The prosecution supported its allegations, mostly, by the Resolution of the Collegiate Board of Directors of the National Health Surveillance Agency No. 15/2012. On the other hand, the defense ratified its positions on the lack of attestations against Porter hospital, defendant in the case. Conclusion: In the simulated jury, the formulation of arguments impacts the teaching-learning process by qualifying future nurses for the requirements of good practices in the processing of health products, in addition to the operationalization, supervision and management within the Material and Sterilization Center.


Objetivo: Reportar la experiencia de un jurado simulado como estrategia didáctico-pedagógica en el proceso de enseñanza-aprendizaje del componente curricular del Centro de Material y Esterilización ­ vinculado al curso de Enfermería de la Faculdade de Enfermagem Nossa Senhora das Graças(FENSG), en la Universidade de Pernambuco, utilizando un abordaje a distancia dadas las circunstancias de la pandemia. Método: Informe de experiencia elaborado por estudiantes y profesores de la Universidad de Pernambuco. La actividad correspondió a un simulacro de jurado basado en material perio-dístico sobre el caso del Hospital Adventista Porter, sobre las consecuencias de supuestas prácticas inapropiadas en el procesamiento de productos de salud. El contenido fue traducido y puesto a disposición de los estudiantes, quienes actuaron como abogados de acusación y defensa en el caso según lo determinen los profesores. Tres etapas componían el simulacro de jurado: acusación, réplica y dúplica, con un tiempo estipulado de 15 minutos para acusación y réplica, y la mitad del tiempo inicial para dúplica en respuesta a la defensa. Resultados: La fiscalía sustentó sus alegatos, en su mayoría, en la Resolución de la Junta Directiva Colegiada no 15/2012. Por otra parte, la defensa ratificó sus posiciones sobre la insuficiencia de atestados contra el hos-pital Porter, demandado en la causa. Conclusión: En el jurado simulado, la formulación de argumentos impacta en el proceso de enseñanza-aprendizaje al calificar a los futuros enfermeros para los requisitos de las buenas prácticas para el procesamiento de productos de salud, además de la operacionaliza-ción, supervisión y gestión dentro del Centro de Material y Esterilización.


Assuntos
Humanos , Escolas de Enfermagem , Esterilização , Educação em Enfermagem , Agência Nacional de Vigilância Sanitária , Aprendizagem
7.
J Virol Methods ; 300: 114382, 2022 02.
Artigo em Inglês | MEDLINE | ID: mdl-34843823

RESUMO

Until mass vaccination befalls, control of the new betacoronavirus-associated severe acute respiratory syndrome pandemic (SARS-CoV-2) is based on decreasing virus circulation by social distancing and blocking transmission foci after diagnosis. Globally adopted SARS-CoV-2 diagnostic criteria embrace viral RNA detection by quantitative reverse-transcription polymerase chain reaction (qRT-PCR) on nasopharynx secretions, which requires healthcare facilities and specialized personnel for sample collection. To develop an alternative protocol, hydrophilic cotton as the material and saliva as the source for biological sample collection in qRT-PCR/RT-endpoint-PCR SARS-CoV-2 diagnostic methods prepared with local consumables were evaluated using 99 archived nasopharynx samples previously diagnosed as positive for SARS-CoV-2 and 111 prospective saliva samples pared with nasopharynx samples from patients attending the local reference ABC Medical School diagnostic laboratory. The kappa agreement coefficient between the SARS-CoV-2 qRT-PCR and RT-endpoint-PCR was k = 0.97 (95 % CI 0.92-1.00) and k = 0.90 (95 % CI 0.81-0.99), respectively, on SARS-CoV-2-positive archived samples, with the initial qRT-PCR CT under 25. The agreement coefficient of the SARS-CoV-2 alternative saliva diagnostic protocol, when used to test the paired nasopharynx samples, was k = 0.79 (95 % CI 0.56-1,00). These data support that the SARS-CoV-2 diagnostic assay based on self-collected saliva on cotton represents an alternative protocol for mass diagnosis and epidemiological studies in low-income regions.


Assuntos
COVID-19 , SARS-CoV-2 , Humanos , Nasofaringe , Pandemias , Estudos Prospectivos , RNA Viral/genética , Saliva , Manejo de Espécimes
8.
J. Bras. Patol. Med. Lab. (Online) ; 58: e4152022, 2022. tab
Artigo em Inglês | LILACS-Express | LILACS | ID: biblio-1375703

RESUMO

ABSTRACT Lung cancer is the first in terms of incidence and mortality, being responsible worldwide for about 1.8 million deaths. In Brazil 31,270 new cases were diagnosed in 2018, 18,740 in men and 12,350 in women. One of the main challenges about lung cancer is performing an early diagnosis, in most cases the disease is detected in the late stages, which implies in poor prognoses. Tumor biomarkers are hugely relevant in early diagnosis, understanding of carcinogenesis, prognostic determination and therapeutic choice. The present paper reviews non-small cell lung cancer biomarkers described in the literature and their diagnostic, prognostic and therapeutic applications, intervention and therapeutic control for individualized therapy. Although there is still a vast universe to be explored, studies reveal a promising future for lung cancer treatment with increasingly personalized and assertive therapies that increase the chances of progression-free survival.


RESUMO O câncer de pulmão é o primeiro em incidência e mortalidade, sendo responsável mundialmente por cerca de 1,8 milhão de mortes. No Brasil, 31.270 casos novos foram diagnosticados em 2018, sendo 18.740 em homens e 12.350 em mulheres. Um dos principais desafios do câncer de pulmão é o diagnóstico precoce, na maioria das vezes a doença é detectada em fases tardias, o que implica em mau prognóstico. Os biomarcadores tumorais são extremamente relevantes no diagnóstico precoce, compreensão da carcinogênese, determinação do prognóstico e escolha terapêutica. O presente trabalho revisa biomarcadores de câncer de pulmão de células não pequenas descritos na literatura e suas aplicações diagnósticas, prognósticas e terapêuticas, intervenção e controle terapêutico para terapia individualizada. Embora ainda exista um vasto universo a ser explorado, estudos revelam um futuro promissor para o tratamento do câncer de pulmão com terapias cada vez mais personalizadas e assertivas que aumentam as chances de sobrevida livre de progressão.

9.
Enferm. foco (Brasília) ; 12(5): 880-886, dez. 2021.
Artigo em Português | LILACS, BDENF - Enfermagem | ID: biblio-1366718

RESUMO

Objetivo: O Aleitamento Materno é uma prática que proporciona muitos benefícios para o binômio mãe-filho e precisa ser cada vez mais orientado, principalmente durante a gestação. Este estudo teve como objetivo compreender como propostas de educação em saúde podem contribuir para a agregação de novos conhecimentos e atitudes positivas das gestantes frente ao aleitamento materno. Métodos: Trata-se do recorte de um trabalho de conclusão de curso de graduação em enfermagem da Universidade Federal de Campina Grande, realizado no ano de 2019, alicerçado no método da pesquisa-ação e analisado por meio do processo metodológico do Discurso do Sujeito Coletivo. Resultados: Após diagnóstico situacional, realizado através de uma entrevista semiestruturada, foram realizados três encontros com as gestantes, onde foram discutidos os sentimentos atrelados ao desejo de amamentar, receios expressados pelas gestantes acerca do aleitamento materno e os direitos das lactantes, respectivamente. Conclusão: As ações educativas possibilitaram a construção de conhecimentos acerca da amamentação por meio do compartilhamento de saberes e troca de experiências entre as gestantes, além disso, foi possível reverter o déficit de saber que foi percebido na fase de avaliação, tornando-as detentoras e multiplicadoras de conhecimentos no seu coletivo. (AU)


Objective: Breastfeeding is a practice that provides many benefits to the mother-child binomial and needs to be increasingly oriented, especially during pregnancy. This study aimed to understand how health education proposals can contribute to the aggregation of new knowledge and positive attitudes of pregnant women towards breastfeeding. Methods: This is the conclusion of a nursing undergraduate course at the Federal University of Campina Grande, carried out in 2019, based on the action research method and analyzed through the methodological process of the Collective Subject Discourse. Results: After a situational diagnosis, conducted through a semistructured interview, three meetings were held with the pregnant women, where the feelings linked to the desire to breastfeed were discussed, fears expressed by pregnant women about breastfeeding and the rights of breastfeeding women, respectively. Conclusion: The educational actions allowed the construction of knowledge about breastfeeding through the sharing of knowledge and exchange of experiences among pregnant women, in addition, it was possible to reverse the deficit of knowledge that was perceived in the evaluation phase, making them holders and multipliers of knowledge in their collective. (AU)


Objetivo: La lactancia materna es una práctica que proporciona muchos beneficios al binomio madre-hijo y necesita estar cada vez más orientada, especialmente durante el embarazo. Este estudio tenía como objetivo comprender cómo las propuestas de educación para la salud pueden contribuir a la agregación de nuevos conocimientos y actitudes positivas de las mujeres embarazadas hacia la lactancia materna. Métodos: Esta es la conclusión de un curso de licenciatura en enfermería en la Universidad Federal de Campina Grande, realizado en 2019, basado en el método de investigación de acción y analizado a través del proceso metodológico del Discurso de la Asignatura Colectiva. Resultados: Después de un diagnóstico situacional, realizado a través de una entrevista semiestructurada, se llevaron a cabo tres reuniones con las mujeres embarazadas, donde se discutieron los sentimientos relacionados con el deseo de amamantar, los temores expresados por las mujeres embarazadas sobre la lactancia materna y los derechos de las mujeres lactantes, respectivamente. Conclusion: Las acciones educativas permitieron la construcción de conocimientos sobre la lactancia materna mediante el intercambio de conocimientos y el intercambio de experiencias entre las mujeres embarazadas, además, fue posible revertir el déficit de conocimiento que se percibió en la fase de evaluación, convirtiéndolas en titulares y multiplicadores de conocimientos en su colectivo. (AU)


Assuntos
Saúde da Mulher , Aleitamento Materno , Gravidez , Educação em Saúde , Enfermagem Obstétrica
10.
J. Hum. Growth Dev. (Impr.) ; 31(3): 476-483, Sep.-Dec. 2021. graf, map, tab
Artigo em Inglês | LILACS, Index Psicologia - Periódicos | ID: biblio-1356367

RESUMO

INTRODUCTION: With the arrival of the SARS-CoV-2 (Coronavirus 2 of severe acute respiratory syndrome) pandemic in Brazil, especially in the city of São Paulo, there was a need to apply social isolation policies associated with testing, covering all municipalities. The Clinical Analysis Laboratory of Centro Universitário FMABC was one of the first laboratories to receive certification and qualification to perform RT-PCR (reverse transcriptase reaction followed by polymerase chain reaction) tests in the metropolitan region of São Paulo OBJECTIVE: Aim to analyze the influence of adopting social isolation on the incidence of positivity in COVID-19 tests in the metropolitan region of São Paulo, Brazil METHODS: a descriptive study carried out from March to May 2020, epidemiological data were collected from each unit served and organized by the data controllership team of the Clinical Analysis Laboratory of FMABC. Epidemiological, demographic, and laboratory data were extracted from the Matrix® outpatient data management system. Clinically suspected cases and confirmed by laboratory tests (RT-PCR and serological tests) were entered. The tests were divided into serological tests using the RT-PCR molecular test, on samples of nasopharyngeal mucosal scrapings collected with sterile Swab RESULTS: It were evaluated PCR test and antibody presence (IgA, IgM and IgG) in blood samples of 16.297 patients. 22.718 tests were performed for the diagnosis of COVID-19, both RT-PCR (10.410 tests) and serological tests to detect anti-SARS-CoV-2 antibodies, IgA, IgM and IgG, a total of 16.297 patients were assessed, 63% women and 37% men. It was observed that the social isolation policies adopted during this period contained the massive expansion of contamination, at least while the social isolation rates were above 55% CONCLUSION: The data of this study demonstrated the effectiveness of social isolation in containing the positive contamination of SARS-CoV-2 in the metropolitan region of São Paulo, at least for the first three months


INTRODUÇÃO: com a chegada da pandemia de SARS-CoV-2 (Coronavirus 2 da síndrome respiratória aguda grave) ao Brasil, especialmente na cidade de São Paulo, houve a necessidade de aplicar medidas de distanciamento social associado a testagem, que abrangesse todos os municípios. A região metropolitana de São Paulo compreende 39 municípios e possui uma rede de laboratórios habilitados a realizar a testagem para a detecção do coronavírus, tanto testes sorológicos quanto moleculares. O Laboratório de Análises Clínicas do Centro Universitário ABC/FMABC foi um dos primeiros laboratórios a receber a certificação e habilitação para realizar os testes RT-PCR (reação da transcriptase reversa seguida pela reação em cadeia da polimerase) na região metropolitana de São Paulo OBJETIVO: analisar a influência da adoção do isolamento social na incidência de positividade nos testes de COVID-19 em região metropolitana de São Paulo, Brasil MÉTODO: estudo descritivo realizado no período de março a maio de 2020, os dados epidemiológicos foram coletados de cada unidade atendida e organizada pela equipe de controladoria de dados do Laboratório de Análises Clínicas da FMABC. Os dados epidemiológicos, demográficos e laboratoriais foram extraídos do sistema Matrix® de gerenciamento de dados ambulatoriais. Foram inseridos os casos clinicamente suspeitos e confirmados por testes de laboratório (RT-PCR e testes sorológicos). Os testes foram divididos em testes sorológicos no teste molecular RT-PCR, em amostras de raspado de mucosa nasofaríngea coletada com Swab estéril RESULTADOS: foram avaliados o teste de RT-PCR e a presença de anticorpos (IgA, IgM e IgG) em amostras de sangue de 16.297 pacientes. Foram realizados 22.718 testes para o diagnóstico de COVID-19, tanto RT-PCR (10.410 testes), quanto testes sorológicos para detecção de anticorpos anti-SARS-CoV-2, IgA, IgM e IgG, um total de 16.297 pacientes foram avaliados, 63% mulheres e 37% homens. Observou-se que as políticas de isolamento social adotadas nesse período continham a expansão massiva da contaminação, pelo menos enquanto as taxas de isolamento social eram superiores a 55% CONCLUSÃO: nossos dados demonstraram a efetividade do isolamento social na retenção da positividade da contaminação do SARS-CoV-2 nas cidades contempladas pelo serviço de testagem do Centro Universitário Saúde ABC, pelo menos nos três primeiros meses


Assuntos
Isolamento Social , Testes Sorológicos , Pandemias , Teste de Ácido Nucleico para COVID-19 , COVID-19 , COVID-19/epidemiologia
11.
J Pediatr (Rio J) ; 2021 Nov 12.
Artigo em Inglês | MEDLINE | ID: mdl-34774468

RESUMO

OBJECTIVE: The nutritional status resultant from dietary habits along with socioeconomic conditions and the school environment are directly related to the individual's health condition not only in their childhood but also throughout adulthood. The aim of this study was to evaluate the effects of socioeconomic factors on the anthropometric profile and to analyze a probable association between this profile and biochemical markers in children attending public daycare centers. METHODS: It is a transversal study developed in a probability sample of clusters of children from 6 months to 5 years old. Anthropometric and socioeconomic data were gathered at the CMEIs, questionnaires on the nutritional status were applied and blood was collected at the Family Health Units (USFs). RESULTS: Female children are three times more likely to be underweight; in families with five members, it is 1/3 more likely that children of higher-educated parents are overweight. Among the results of the biochemical tests, hypervitaminosis A was a relevant aspect, positively correlating with copper (p=0.005) and zinc (p=0.008). CONCLUSION: Therefore, since the influence of the family is an important predictor of overweight and its future outcomes related to nutritional deficiencies and inadequate dietary intake, educational interventions are vital as a way to pave the path to prevention.

12.
J Clin Pathol ; 2021 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-34782424

RESUMO

AIMS: To determine the profile of COX-2 gene expression in patients with prostate cancer attended at the ABC University Health Center outpatient clinic and correlate the results with patients' anatomopathological examinations. Prostate cancer is the sixth most common type of cancer worldwide and the second in Brazil. COX-2 expression is associated with an unfavourable prognosis. METHODS: 15.0 mL of peripheral blood were collected from 24 patients and 25 healthy men. RNA extraction was performed using the QIAamp RNA Blood Mini Kit. Complementary DNA synthesis was performed using SuperScript II RNAse Reverse Transcriptase. Quantitative real-time PCR was performed with specific COX-2 oligonucleotides and the endogenous GAPDH gene. RESULTS: The mean age of the patients was 69 years old. The Gleason scoring system showed 37.5% of patients with Gleason 6 (slow growth, low risk), 45.8% with Gleason 7 (intermediate risk) and 16.7% with Gleason 8 or 9 (risk of high-grade cancer). The median COX-2 expression in the study group was 0.97, while in the control group it was 0.11 (p<0.045). CONCLUSIONS: Patients with prostate cancer showed higher COX-2 expression at diagnosis compared with the control group. Since COX-2 detection associated with prostate-specific antigen dosage shows promise as a biomarker for diagnosis and prognosis in patients with prostate cancer, further research is required to confirm these findings.

13.
J Med Food ; 2021 Aug 24.
Artigo em Inglês | MEDLINE | ID: mdl-34432547

RESUMO

New perspectives arise in the therapeutic practice for cancer, with the objective to not only treat patients, but also improve their quality of life. Guarana, a plant from Brazilian Amazon presents a wide range of pharmacological actions. This study evaluated the effect of Guarana (Paullinia cupana) extract, pure and dry Guarana (PC-18) extract and magnesium chloride (MgCl2) in mice of the Balb/c strain inoculated with the Ehrlich tumor regarding gene expression of inflammatory markers transforming growth factor-ß1 and tumor necrosis factor alpha and oxidative stress (OS) and fatigue, superoxide dismutase, catalase, and glutathione peroxidase 4 and analyzed myelotoxicity and hepatotoxicity. After euthanasia, blood was collected to analyze the complete blood count and measured the levels of liver enzymes (alanine aminotransferase and aspartate aminotransferase). Hepatoprotective actions of the crude extract of P. cupana and PC-18 extract were noticed. The PC-18 and MgCl2 group showed the best result regarding animal welfare. There were no associations between compounds and gene expression regarding fatigue and OS. PC-18 reduced the tumor and may have an antitumor action. The crude extract of Guarana presented hepatoprotective action.

14.
Adv Exp Med Biol ; 1306: 13-27, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33959903

RESUMO

Kidney diseases are conditions that increase the morbidity and mortality of those afflicted. Diagnosis of these conditions is based on parameters such as the glomerular filtration rate (GFR), measurement of serum and urinary creatinine levels and equations derived from these measurements (Wasung, Chawla, Madero. Clin Chim Acta 438:350-357, 2015). However, serum creatinine as a marker for measuring renal dysfunction has its limitations since it is altered in several other physiological situations, such as in patients with muscle loss, after intense physical exercise or in people on a high protein diet (Riley, Powers, Welch. Res Q Exerc Sport 52(3):339-347, 1981; Juraschek, Appel, Anderson, Miller. Am J Kidney Dis 61(4):547-554, 2013). Besides the fact that serum creatinine is a marker that indicates glomerular damage, it is necessary the discovery of new biomarkers that reflect not only glomerular damage but also tubular impairment. Recent advances in Molecular Biology have led to the generation or identification of new biomarkers for kidney diseases such as: Acute Kidney Failure (AKI), chronic kidney disease (CKD), nephritis or nephrotic syndrome. There are recent markers that have been used to aid in diagnosis and have been shown to be more sensitive and specific than classical markers, such as neutrophil gelatinase associated lipocalin (NGAL) or kidney injury molecule-1 (KIM-1) (Wasung, Chawla, Madero. Clin Chim Acta 438:350-357, 2015; George, Gounden. Adv Clin Chem 88:91-119, 2019; Han, Bailly, Abichandani, Thadhani, Bonventre. Kidney Int 62(1):237-244, 2002; Fontanilla, Han. Expert Opin Med Diagn 5(2):161-173, 2011). However, early diagnostic biomarkers are still necessary to assist the intervention and monitor of the progression of these conditions.


Assuntos
Injúria Renal Aguda , Lipocalinas , Proteínas de Fase Aguda , Biomarcadores , Creatinina , Taxa de Filtração Glomerular , Humanos , Proteínas Proto-Oncogênicas
15.
Eur J Pharmacol ; 905: 174180, 2021 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-34015319

RESUMO

Systemic arterial hypertension (SAH) is a chronic disease of multifactorial origin and one of the main risk factors for major adverse cardiovascular events (MACE), which are the leading causes of morbidity and mortality worldwide. The pharmacological treatment of SAH involves five main classes of drugs, and Nebivolol (NEB) is one of those drugs, belonging to the class of third generation ß1-adrenoceptors selective blockers. NEB is composed of a racemic mixture of two enantiomers: d-nebivolol, which exerts antagonist effects on ß1-adrenoceptors, and l-nebivolol, a vascular ß3 receptor agonist. There are several studies that report different actions of NEB, not only for the treatment of SAH, but also as an antioxidant agent or even as a protector of renal damage. The aim of this systematic review was to investigate the available evidence regarding the effects of NEB on kidney diseases, evaluating its possible renoprotective action.


Assuntos
Nebivolol/farmacologia , Nebivolol/uso terapêutico , Substâncias Protetoras/farmacologia , Substâncias Protetoras/uso terapêutico , Insuficiência Renal Crônica/tratamento farmacológico , Antagonistas de Receptores Adrenérgicos beta 3/farmacologia , Antagonistas de Receptores Adrenérgicos beta 3/uso terapêutico , Animais , Humanos , Hipertensão/tratamento farmacológico , Menopausa/efeitos dos fármacos , Receptores Adrenérgicos beta 3
16.
Sci Rep ; 11(1): 8724, 2021 04 22.
Artigo em Inglês | MEDLINE | ID: mdl-33888756

RESUMO

In tumor cells, higher expression of glucose transporter proteins (GLUT) and carbonic anhydrases (CAIX) genes is influenced by hypoxia-induced factors (HIF).Thus, we aimed to study the expression profile of these markers in sequential peripheral blood collections performed in breast cancer patients in order to verify their predictive potential in liquid biopsies. Gene expressions were analyzed by qPCR in tumor and blood samples from 125 patients and 25 healthy women. Differential expression was determined by the 2(-ΔCq) method. Expression of HIF-1α and GLUT1 in the blood of breast cancer patients is significantly higher (90-91 and 160-161 fold increased expression, respectively; p < 0.0001) than that found in healthy women. Their diagnostic power was confirmed by ROC curve. CAIX is also more expressed in breast cancer women blood, but its expression was detected only in a few samples. But none of these genes could be considered predictive markers. Therefore, evaluation of the expression of HIF-1α and GLUT1 in blood may be a useful laboratory tool to complement the diagnosis of breast cancer, in addition to being useful for follow-up of patients and of women with a family history of breast cancer.


Assuntos
Neoplasias da Mama/diagnóstico , Neoplasias da Mama/genética , Anidrase Carbônica IX/genética , Hipóxia Celular/genética , Regulação Neoplásica da Expressão Gênica , Transportador de Glucose Tipo 1/genética , Subunidade alfa do Fator 1 Induzível por Hipóxia/genética , Biópsia Líquida/métodos , Estudos de Casos e Controles , Feminino , Humanos , Pessoa de Meia-Idade
17.
J Diet Suppl ; : 1-15, 2021 Mar 22.
Artigo em Inglês | MEDLINE | ID: mdl-33749469

RESUMO

Magnesium supplementation may be beneficial for cancer patients due to its action as a modulator of cell proliferation and metabolism and its anti-inflammatory effect. Tumor metabolism can influence the bioavailability and absorption of nutrients, leading to an increase in the individual's nutritional needs. In this work, the effects of supplementing different dosages of magnesium chloride in mice with solid Ehrlich's tumors were investigated by analyzing their hematological, inflammatory and anthropometric biomarkers. Three dosages of magnesium chloride (MgCl2) were administered for 28 consecutive days. Animal welfare was assessed according to the criteria stipulated by the National Center for the Replacement, Refinement, and Reduction of Animals in Research (NC3Rs). The inverted grid method was used to analyze muscle strength and fatigue. Difference in expression of the Tumor Necrosis Factor (TNF-α) and the Growth Transformation Factor (TGF-ß1) genes was determined by the 2-ΔCt method. The hematological evaluation consisted of the erythrogram, white blood cell and platelet counts were used for the hematological evaluation and treatment cytotoxicity. Difference in the expression of the TNF-α and TGF-ß genes showed that the group that received a high dose of magnesium had a decrease in TNF-α and RNL, an improvement in well-being with a tendency to increase muscle strength and less tumor progression according to the days of treatment. The group that received a low dosage of magnesium had a smaller tumor volume and a more controlled tumor growth according to the days. The group that received an intermediate dosage presented cytotoxicity.

18.
Sci Rep ; 11(1): 6282, 2021 03 18.
Artigo em Inglês | MEDLINE | ID: mdl-33737530

RESUMO

The high-fat diet (HFD) stimulates an increase in lipids and can be prejudicial for harmful to prostatic morphogenesis. Polyunsaturated fatty acid (PUFAs) have anti-inflammatory and antioxidant action in some types of cancer. The combination of aerobic physical exercise and PUFA can be more effective and reduce the risk of death. The study evaluates the effects of aerobic physical exercise associated with omega-3 (fish and chia oils), on the ventral prostate of Wistar rats those fed with HFD. Here, we report that HFD modified the final body weight and the weight gain, decreased the expression of the androgen receptor and increased prostatic inflammation via TNF-α produced damage prostatic like intraepithelial neoplasia. The supplementation with fish oil decreases final body weight, reduced BCL-2 and inflammation compared to chia oil; aerobic physical exercise associated with fish oil reduced lipids circulant and prostatic, increased proteins pro-apoptotic expression and reduced IL-6 (p < 0.0001) and TNF-α potentiating the CAT (p = 0.03) and SOD-1 (p = 0.001) expression. Additionally, the chia oil increased the NRF-2 (p < 0.0001) and GSS (p = 0.4) genes. PUFAs reduced the damage caused by excessive high-fat diet in the prostate so that there is greater effectiveness in omega-3 intake, it is necessary to associate with aerobic physical exercise.


Assuntos
Suplementos Nutricionais , Terapia por Exercício/métodos , Ácidos Graxos Ômega-3/uso terapêutico , Obesidade/dietoterapia , Obesidade/metabolismo , Condicionamento Físico Animal/métodos , Próstata/metabolismo , Animais , Terapia Combinada/métodos , Dieta Hiperlipídica/efeitos adversos , Masculino , Obesidade/etiologia , Oxirredução , Ratos , Ratos Wistar , Receptores Androgênicos/metabolismo , Resultado do Tratamento , Fator de Necrose Tumoral alfa/metabolismo , Ganho de Peso , Perda de Peso
19.
Rev. bras. promoç. saúde (Impr.) ; 34(1): 1-9, 17/02/2021.
Artigo em Inglês, Português | LILACS | ID: biblio-1342975

RESUMO

Objetivo: Identificar experiências e atitudes de gestantes acerca do aleitamento materno. Métodos: Estudo qualitativo mediatizado por uma pesquisa-ação, realizado com 12 gestantes, em duas Unidades Básicas de Saúde da cidade de Cajazeiras, Paraíba, com a função de identificar seus conhecimentos e experiências acerca do processo de amamentação. A coleta de dados foi realizada por meio de entrevistas semiestruturadas com questionamentos sobre os benefícios da amamentação, direitos e deveres das lactantes e experiências prévias, a fim de identificar melhor o grupo analisado. Após as entrevistas, as respostas foram transcritas e analisadas pelo uso da técnica do Discurso do Sujeito Coletivo, e utilizadas para nortear o planejamento e realização de intervenções de educação em saúde visando solucionar a deficiência de conhecimento das participantes acerca da amamentação. Resultados: As participantes possuem conhecimento acerca dos benefícios do aleitamento materno para o binômio mãe-filho e acerca das complicações que podem ser desencadeadas quando a amamentação não se dá de forma correta. Entretanto, houve déficit de conhecimento no que se refere aos benefícios da amamentação para a mãe e percebeu-se que a motivação de amamentar ainda é muito influenciada por mitos e crenças locais que levam ao desmame precoce. Conclusão: Observaram-se as lacunas existentes no conhecimento das gestantes investigadas sobre o aleitamento materno no que se refere aos benefícios maternos da prática em questão. Além disso, observou-se também os principais mitos e crenças que levam ao desmame precoce.


Objective: To identify experiences and attitudes of pregnant women about breastfeeding. Methods: Qualitative study was mediated by action research, carried out with 12 pregnant women in two Basic Health Units in Cajazeiras, Paraíba, to identify their knowledge and experiences about the breastfeeding process. Data collection was performed through semi-structured interviews with questions about the benefits of breastfeeding, rights, and duties of breastfeeding women, and previous experiences to better identify the analyzed group. After the interviews, the answers were transcribed and analyzed using the Collective Subject Discourse technique and used to guide the planning and implementation of health education interventions to solve the participants' lack of knowledge about breastfeeding. Results: The participants know the benefits of breastfeeding for the mother-child binomial, and the complications can be triggered when breastfeeding does not occur correctly. However, there was a lack of knowledge regarding the benefits of breastfeeding for the mother, and it was noticed the motivation to breastfeed is still strongly influenced by local myths and beliefs that lead to early weaning. Conclusion: The gaps in the knowledge of the investigated pregnant women about breastfeeding were observed regarding the maternal benefits of the practice in question. In addition, the major myths and beliefs that lead to early weaning were also observed.


Objetivo: Identificar las experiencias y actitudes de embarazadas sobre la lactancia materna. Métodos: Estudio cualitativo a través de una investigación-acción realizada con 12 embarazadas de dos Unidades Básicas de Salud de la ciudad de Cajazeiras, Paraíba, con el objetivo de identificar sus conocimientos y experiencias sobre el proceso de la lactancia materna. La recogida de datos se dio a través de entrevistas semiestructuradas con preguntas sobre los beneficios de la lactancia materna, los derechos y deberes de las lactantes y sus experiencias anteriores para identificar mejor el grupo analizado. Después de las entrevistas, las respuestas han sido transcritas y analizadas con la técnica del Discurso del Sujeto Colectivo y utilizadas para orientar el planeamiento y la realización de las intervenciones de educación en salud para solucionar la deficiencia del conocimiento de las participantes sobre la lactancia materna. Resultados: Las participantes tienen conocimiento sobre los beneficios de la lactancia materna para el binomio madre-hijo y sobre las complicaciones que pueden desencadenarse cuando la lactancia materna no se da de manera correcta. Sin embargo, hubo déficit del conocimiento sobre los beneficios de la lactancia materna para la madre y se percibió que la motivación para la lactancia aún tiene influencia de los mitos y creencias locales que llevan al destete temprano. Conclusión: Se ha observado las brechas que hay en el conocimiento de las embarazadas investigadas sobre la lactancia materna respecto los beneficios maternos de esa práctica. Además, se observó también los principales mitos y creencias que llevan al destete temprano.


Assuntos
Aleitamento Materno , Educação em Saúde , Saúde da Mulher , Enfermagem , Pesquisa Qualitativa
20.
Av. enferm ; 39(1): 112-120, 01 de enero de 2021.
Artigo em Português | LILACS, BDENF - Enfermagem, COLNAL | ID: biblio-1151189

RESUMO

Objetivo: refletir sobre a violência simbólica no campo familiar na (des)estruturação do habitus do adolescente a partir da série televisiva Sex education. Síntese de conteúdo: estudo teórico-reflexivo, tendo por base livros e artigos que demonstrem os conceitos de campo, capital, habitus, poder e violência simbólica do filósofo Pierre Bourdieu e a série de televisão Sex education, que possibilita um novo olhar das relações complexas e das estruturações identitárias existentes no contexto familiar. Reconhecendo que a violência simbólica se expressa por meio de relações de poder manifestadas entre o dominador e o dominado, é perceptível a incorporação e a legitimação de um discurso marginalizado, por parte de indivíduos pertencentes a uma classe de menor empoderamento, aceitando essa realidade social de forma natural. Conclusões: percebese que é necessário um olhar crítico sobre as relações interpessoais no contexto familiar, uma vez que é na socialização familiar que se tem o início da incorporação e da construção do habitus dos indivíduos, e que a violência simbólica, originada de um campo de submissão, pode ser tão prejudicial quanto os outros tipos de violência visíveis, já que afeta decisivamente a construção e o desenvolvimento dos adolescentes


Objective: To reflect on the symbolic violence in the family field and the (de)structuring of adolescent habitus based on the television series Sex education. Content synthesis: Theoretical and reflective study based on books and articles by philosopher Pierre Bourdieu, that address the concepts of field, capital, habitus, power and symbolic violence, and the television series Sex Education, which allows a new look at the complex relationships and existing identity structures in the family context. Recognizing that symbolic violence expresses itself through manifested power relations between the dominator and the dominated, it is noticeable the incorporation and legitimization of a marginalized discourse by individuals belonging to a less empowered class, who accept this social reality in a natural way. Conclusions:A critical look is required on interpersonal relation-ships in the family context, since it is in family socialization that the incorporation and construction of the habitus of individuals begins. Besides, it is observed that symbolic violence originated in a submission environment can be as damaging as other types of visible violence, since this decisively affects the construction and development of adolescents.


Objetivo: reflexionar sobre la violencia simbólica en el ámbito familiar y la (des)estructuración del habitus del adolescente con base en la serie de televisión Sex education. Síntesis de contenido: estudio teórico-reflexivo basado en libros y artículos del filósofo Pierre Bourdieu, que abordan los conceptos de campo, capital, habitus, poder y violencia simbólica, y la serie de tele-visión Sex education, la cual permite una nueva mirada a las complejas relaciones y estructuras de identidad existentes en el contexto familiar. Reconociendo que la violencia simbólica se expresa a través de las relaciones de poder manifestadas entre el dominador y el dominado, es notable la incorporación y la legitimación de un discurso marginado por parte de individuos pertenecientes a una clase menos empoderada, que acepta esta realidad social de forma natural. Conclusiones: se evidencia la necesidad de una mirada crítica de las relaciones interpersonales en el contexto familiar, ya que es en la socialización familiar que comienza la incorporación y construcción del habitus de los individuos. Así mismo, se observa que la violencia simbólica, originada en un entorno de sumisión, puede ser tan perjudicial como otros tipos de violencia visible, pues esta afecta decisivamente la construcción y el desarrollo de los adolescentes.


Assuntos
Humanos , Violência , Comportamento do Adolescente , Poder Familiar , Violência Doméstica
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