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1.
J Immunol Methods ; 433: 31-7, 2016 06.
Artigo em Inglês | MEDLINE | ID: mdl-26928648

RESUMO

The etiological diagnosis of infection by Streptococcus pneumoniae in children is difficult, and the use of indirect techniques is frequently warranted. We aimed to study the use of pneumococcal proteins for the serological diagnosis of pneumococcal infection in children with pneumonia. We analyzed paired serum samples from 13 Brazilian children with invasive pneumococcal pneumonia (positive control group) and 23 Finnish children with viral pharyngitis (negative control group), all aged <5years-old. Children with pharyngitis were evaluated for oropharyngeal colonization, and none of them carried S. pneumoniae. We used a multiplex bead-based assay with eight proteins: Ply, CbpA, PspA1 and 2, PcpA, PhtD, StkP and PcsB. The optimal cut-off for increase in antibody level for the diagnosis of pneumococcal infection was determined for each antigen by ROC curve analysis. The positive control group had a significantly higher rate of ≥2-fold rise in antibody levels against all pneumococcal proteins, except Ply, compared to the negative controls. The cut-off of ≥2-fold increase in antibody levels was accurate for pneumococcal infection diagnosis for all investigated antigens. However, there was a substantial increase in the accuracy of the test with a cut-off of ≥1.52-fold rise in antibody levels for PcpA. When using the investigated protein antigens for the diagnosis of pneumococcal infection, the detection of response against at least one antigen was highly sensitive (92.31%) and specific (91.30%). The use of serology with pneumococcal proteins is a promising method for the diagnosis of pneumococcal infection in children with pneumonia. The use of a ≥2-fold increase cut-off is adequate for most pneumococcal proteins.


Assuntos
Anticorpos Antibacterianos/sangue , Imunoensaio/métodos , Pneumonia Pneumocócica/diagnóstico , Antígenos de Bactérias/imunologia , Proteínas de Bactérias/imunologia , Brasil , Pré-Escolar , Feminino , Humanos , Lactente , Masculino , Microesferas , Estudos Prospectivos , Curva ROC , Sensibilidade e Especificidade , Testes Sorológicos , Streptococcus pneumoniae/isolamento & purificação
2.
Rev. bras. cardiol. (Impr.) ; 26(2): 106-111, mar.-abr. 2013. tab, graf
Artigo em Português | LILACS | ID: lil-685720

RESUMO

Fundamentos: A insuficiência cardíaca (IC) é uma síndrome crônica, de tratamento complexo; de tal modo permite que estratégias de educação em saúde tenham importante impacto na qualidade de vida dos pacientes. Objetivo: Avaliar a eficácia de palestra realizada na sala de espera de um ambulatório para pacientes com IC. Métodos: Palestra educativa foi realizada para pacientes que aguardavam o atendimento, com duração média de 40 minutos. Antes e após a palestra, os pacientes responderam ao mesmo questionário, composto por 10 perguntas objetivas, concernentes à IC. Dados sociodemográficos foram coletados na ocasião do preenchimento do questionário e os dados clínicos durante a consulta. Resultados: 187 pacientes responderam ao questionário inicial e 114 ao questionário final. Foi encontrada uma melhora significativa no desempenho dos pacientes após a palestra (79,3% vs. 92,8%, p<0,001). Conclusões: Palestras constituem uma importante estratégia educacional, são de simples execução e apresentam impacto imediato e significativo no conhecimento dos pacientes sobre IC.


Background: Heart failure (HF) is a chronic syndrome and of complex treatment. So that, it allows health education strategies to have important impact on the management of these patients. Objective: To evaluate the efficacy of educational activities on the waiting room of a specialized clinic for patients with HF. Methods: While waiting for their appointments at the clinic, the patients took part on an educational activity with main duration of 40 minutes. They answered questionnaire (consisting of 10 questions) both before and after the lectures about HF. Sociodemographic data were collected during the questionnaire application and the clinical data during the medical appointment. Results: 187 patients answered the initial questionnaire and 114 answered both of them. There was a significant improvement of the questionnaire's results after the lectures (79.3% vs 92.8%, p<0.001). Conclusions: Lectures performed at the waiting room of a specialized clinic represent an important educational strategy of simple execution and with a significant immediate impact on the patient's knowledge about HF.


Assuntos
Humanos , Masculino , Feminino , Pessoa de Meia-Idade , Ambulatório Hospitalar/tendências , Insuficiência Cardíaca/complicações , Insuficiência Cardíaca/diagnóstico , Insuficiência Cardíaca/mortalidade , Inquéritos e Questionários , Guias como Assunto/normas , Estratégias , Ensino
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