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1.
Arch Oral Biol ; 112: 104670, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32058859

RESUMO

OBJECTIVES: To investigate the regulation of inflammatory and osteoclastogenic signaling by 5-lipoxygenase (5-LO) in apical periodontitis induced by oral contamination of dental root canals in mice. DESIGN: Apical periodontitis was induced in 5-lipoxygenase enzyme knockout (129-Alox5tm1Fun) and 129 wild-type mice (n = 96) by exposure of the dental root canal to the oral cavity. After 7, 14, 21, and 28 days, the animals were euthanized and the tissues removed (n = 12 teeth per period) for histopathological and histometric analyses (hematoxylin and eosin [HE]), evaluation of osteoclastogenic activity (tartrate-resistant acid phosphatase enzyme [TRAP]), and determination of inflammatory and osteoclastogenic signaling (qRT-PCR). RESULTS: Oral contamination of dental root canals induced recruitment of neutrophils and osteoclasts to the periodontal ligament, resulting in bone resorption. Absence of 5-LO did not impair neutrophil recruitment while osteoclastic formation was increased. Nonetheless, early bone resorption progressed similarly to lesions in wild-type animals. Interestingly, in the absence of 5-LO, the synthesis of mRNAs for cytokines, chemokines, and their receptors was significantly reduced while that of regulators of osteoclastogenesis (RANK, RANKL, and OPG) was increased in comparison with the corresponding levels in wild-type animals. CONCLUSIONS: The 5-LO pathway plays a role in the stimulation of inflammatory mediator synthesis and inhibition of osteoclastogenesis in apical periodontitis in mice. However, the paradoxical inflammatory-osteoclastogenic signaling did not impair inflammatory cell recruitment and bone resorption during early development of the disease.


Assuntos
Araquidonato 5-Lipoxigenase/genética , Reabsorção Óssea , Osteogênese , Osteoprotegerina/metabolismo , Periodontite Periapical/genética , Animais , Inflamação , Camundongos , Camundongos Knockout , Osteoclastos , Periodontite Periapical/fisiopatologia , Ligante RANK/metabolismo , Receptor Ativador de Fator Nuclear kappa-B/metabolismo
2.
Braz Dent J ; 30(5): 484-490, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31596333

RESUMO

Papain-based gel is used for chemical-mechanical caries removal and present antimicrobial and anti-inflammatory activities. However, its effects on dental pulp cells and on macrophages remains largely unknown. Therefore, the aim of this study was to investigate whether the papain-based gel Papacárie Duo® acts as an immunomodulator in lipopolysaccharide (LPS)-activated macrophages and its effects on dental pulp cells . J774.1 macrophage and OD-21 dental pulp cells were stimulated with 0.5% and 5% of Papacárie Duo®, following pre-treatment or not with LPS. After 24 h, a lactate dehydrogenase assay was used to measure cytotoxicity, a tetrazolium-based colorimetric assay (MTT) was used to measure cell viability, and qRT-PCR was used to analyze relative gene expression of Ptgs2, Il10, Tnf, Mmp9, Runx2, Ibsp and Spp1. Papacárie Duo® was cytotoxic and reduced cell viability at 5% but not at 0.5% in both cultures. In macrophages, Papacárie Duo® increased the expression Il10 and LPS-induced Ptgs2, but it did not affect Tnf or Mmp9. In OD-21 cells, Papacárie Duo® inhibited Runx2 and Ibsp expression, but stimulated Spp1 expression. Papain-based gel presented a concentration dependent cytotoxicity, without affecting cell viability, for dental pulp cells and macrophages. Interestingly, the gel presented an inhibitory effect on pulp cell differentiation but modulated the activation of macrophages stimulated with LPS. We speculate that in dental pulp tissue, Papacárie Duo® would impair reparative dentinogenesis but could activate macrophages to perform their role in defense and inflammation.


Assuntos
Cárie Dentária , Papaína , Preparo da Cavidade Dentária , Polpa Dentária , Humanos , Macrófagos
3.
Braz. dent. j ; 30(5): 484-490, Sept.-Oct. 2019. graf
Artigo em Inglês | LILACS-Express | ID: biblio-1039137

RESUMO

Abstract Papain-based gel is used for chemical-mechanical caries removal and present antimicrobial and anti-inflammatory activities. However, its effects on dental pulp cells and on macrophages remains largely unknown. Therefore, the aim of this study was to investigate whether the papain-based gel Papacárie Duo® acts as an immunomodulator in lipopolysaccharide (LPS)-activated macrophages and its effects on dental pulp cells . J774.1 macrophage and OD-21 dental pulp cells were stimulated with 0.5% and 5% of Papacárie Duo®, following pre-treatment or not with LPS. After 24 h, a lactate dehydrogenase assay was used to measure cytotoxicity, a tetrazolium-based colorimetric assay (MTT) was used to measure cell viability, and qRT-PCR was used to analyze relative gene expression of Ptgs2, Il10, Tnf, Mmp9, Runx2, Ibsp and Spp1. Papacárie Duo® was cytotoxic and reduced cell viability at 5% but not at 0.5% in both cultures. In macrophages, Papacárie Duo® increased the expression Il10 and LPS-induced Ptgs2, but it did not affect Tnf or Mmp9. In OD-21 cells, Papacárie Duo® inhibited Runx2 and Ibsp expression, but stimulated Spp1 expression. Papain-based gel presented a concentration dependent cytotoxicity, without affecting cell viability, for dental pulp cells and macrophages. Interestingly, the gel presented an inhibitory effect on pulp cell differentiation but modulated the activation of macrophages stimulated with LPS. We speculate that in dental pulp tissue, Papacárie Duo® would impair reparative dentinogenesis but could activate macrophages to perform their role in defense and inflammation.


Resumo O gel à base de papaína é utilizando para remoção químico-mecânica do tecido cariado e apresenta propriedades antimicrobianas e anti-inflamatórias Entretanto, seu efeito sobre as células da polpa dentárias e macrófagos é desconhecido. Portanto, o objetivo deste estudo foi investigar o efeito de um gel de papaína (Papacárie Duo®) em células indiferenciadas da polpa dentária e a capacidade de induzir a ativação e síntese de mediadores inflamatórios por macrófagos estimulados com lipopolissacarídeo bacteriano (LPS). O gel de papaína foi diluído nas concentrações de 0,5 e 5%. Células indiferenciadas da polpa dentária OD-21 e macrófagos J774.1 foram mantidos em cultura com os diferentes estímulos por um período de estimulação de 24 h para realização do teste de citotoxicidade (Ensaio LDH) e para avaliação da viabilidade celular (Ensaio Colorimétrico MTT). A seguir foi realizada avaliação da expressão gênica relativa dos genes Ibsp, Runx2 e Spp1 em células OD-21; e dos genes Il10, Mmp9, Ptgs2 e Tnf em células J774.1, pelo método de transcrição reversa e reação em cadeia de polimerase em tempo real (qRT-PCR), após estimulação pelo período de 24 h. O extrato do gel diluído a 5% foi citotóxico às células da polpa dental, reduziu a viabilidade celular, inibiu a expressão de Runx2 e Ibsp e estimulou a expressão de Spp1. Em macrófagos, o extrato do gel foi citotóxico e reduziu a viabilidade celular na concentração de 5%. O LPS inibiu a viabilidade celular na presença ou não do extrato do gel, sem apresentar citotoxicidade. O extrato do gel induziu a expressão de Ptgs2 e Il10, sem alterar Tnf e Mmp9. O extrato do gel de papaína foi citotóxico, dependente da concentração, tanto em células da polpa dentária como em macrófagos, sem alterar a viabilidade celular. Interessantemente, apresentou efeito inibitório na diferenciação de células da polpa dentária e modulou a ativação de macrófagos estimulados com LPS. No tecido pulpar, o Papacárie Duo® poderia impedir a dentinogênese de reparação, porém ativar macrófagos para desempenhar seu papel na inflamação e defesa.

4.
J Appl Oral Sci ; 27: e20180641, 2019 Jun 03.
Artigo em Inglês | MEDLINE | ID: mdl-31166414

RESUMO

OBJECTIVES: Infection, inflammation and bone resorption are closely related events in apical periodontitis development. Therefore, we sought to investigate the role of cyclooxygenase (COX) in osteoclastogenesis and bone metabolism signaling in periapical bone tissue after bacterial lipopolysaccharide (LPS) inoculation into root canals. METHODOLOGY: Seventy two C57BL/6 mice had the root canals of the first molars inoculated with a solution containing LPS from E. coli (1.0 mg/mL) and received selective (celecoxib) or non-selective (indomethacin) COX-2 inhibitor. After 7, 14, 21 and 28 days the animals were euthanized and the tissues removed for total RNA extraction. Evaluation of gene expression was performed by qRT-PCR. Statistical analysis was performed using analysis of variance (ANOVA) followed by post-tests (α=0.05). RESULTS: LPS induced expression of mRNA for COX-2 (Ptgs2) and PGE2 receptors (Ptger1, Ptger3 and Ptger4), indicating that cyclooxygenase is involved in periapical response to LPS. A signaling that favours bone resorption was observed because Tnfsf11 (RANKL), Vegfa, Ctsk, Mmp9, Cd36, Icam, Vcam1, Nfkb1 and Sox9 were upregulated in response to LPS. Indomethacin and celecoxib differentially modulated expression of osteoclastogenic and other bone metabolism genes: celecoxib downregulated Igf1r, Ctsk, Mmp9, Cd36, Icam1, Nfkb1, Smad3, Sox9, Csf3, Vcam1 and Itga3 whereas indomethacin inhibited Tgfbr1, Igf1r, Ctsk, Mmp9, Sox9, Cd36 and Icam1. CONCLUSIONS: We demonstrated that gene expression for COX-2 and PGE2 receptors was upregulated after LPS inoculation into the root canals. Additionally, early administration of indomethacin and celecoxib (NSAIDs) inhibited osteoclastogenic signaling. The relevance of the cyclooxygenase pathway in apical periodontitis was shown by a wide modulation in the expression of genes involved in both bone catabolism and anabolism.


Assuntos
Inibidores de Ciclo-Oxigenase/farmacologia , Cavidade Pulpar/metabolismo , Lipopolissacarídeos/farmacologia , Osteogênese/fisiologia , Tecido Periapical/efeitos dos fármacos , Tecido Periapical/metabolismo , Prostaglandina-Endoperóxido Sintases/fisiologia , Animais , Anti-Inflamatórios não Esteroides/farmacologia , Reabsorção Óssea/metabolismo , Celecoxib/farmacologia , Ciclo-Oxigenase 2/análise , Escherichia coli/metabolismo , Expressão Gênica , Indometacina/farmacologia , Lipopolissacarídeos/análise , Masculino , Camundongos Endogâmicos C57BL , Osteogênese/efeitos dos fármacos , Prostaglandina-Endoperóxido Sintases/análise , Prostaglandina-Endoperóxido Sintases/efeitos dos fármacos , Receptores de Prostaglandina E/análise , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Fatores de Tempo , Regulação para Cima
5.
J. appl. oral sci ; 27: e20180641, 2019. tab, graf
Artigo em Inglês | LILACS-Express | ID: biblio-1012519

RESUMO

Abstract Objectives: Infection, inflammation and bone resorption are closely related events in apical periodontitis development. Therefore, we sought to investigate the role of cyclooxygenase (COX) in osteoclastogenesis and bone metabolism signaling in periapical bone tissue after bacterial lipopolysaccharide (LPS) inoculation into root canals. Methodology: Seventy two C57BL/6 mice had the root canals of the first molars inoculated with a solution containing LPS from E. coli (1.0 mg/mL) and received selective (celecoxib) or non-selective (indomethacin) COX-2 inhibitor. After 7, 14, 21 and 28 days the animals were euthanized and the tissues removed for total RNA extraction. Evaluation of gene expression was performed by qRT-PCR. Statistical analysis was performed using analysis of variance (ANOVA) followed by post-tests (α=0.05). Results: LPS induced expression of mRNA for COX-2 (Ptgs2) and PGE2 receptors (Ptger1, Ptger3 and Ptger4), indicating that cyclooxygenase is involved in periapical response to LPS. A signaling that favours bone resorption was observed because Tnfsf11 (RANKL), Vegfa, Ctsk, Mmp9, Cd36, Icam, Vcam1, Nfkb1 and Sox9 were upregulated in response to LPS. Indomethacin and celecoxib differentially modulated expression of osteoclastogenic and other bone metabolism genes: celecoxib downregulated Igf1r, Ctsk, Mmp9, Cd36, Icam1, Nfkb1, Smad3, Sox9, Csf3, Vcam1 and Itga3 whereas indomethacin inhibited Tgfbr1, Igf1r, Ctsk, Mmp9, Sox9, Cd36 and Icam1. Conclusions: We demonstrated that gene expression for COX-2 and PGE2 receptors was upregulated after LPS inoculation into the root canals. Additionally, early administration of indomethacin and celecoxib (NSAIDs) inhibited osteoclastogenic signaling. The relevance of the cyclooxygenase pathway in apical periodontitis was shown by a wide modulation in the expression of genes involved in both bone catabolism and anabolism.

6.
Braz. dent. j ; 28(3): 296-300, May-June 2017. graf
Artigo em Inglês | LILACS | ID: biblio-888648

RESUMO

Abstract The aim of this study was to evaluate osteoclastogenesis signaling in midpalatal suture after rapid maxillary expansion (RME) in rats. Thirty male Wistar rats were randomly assigned to two groups with 15 animals each: control (C) and RME group. RME was performed by inserting a 1.5-mm-thick circular metal ring between the maxillary incisors. The animals were euthanized at 3, 7 and 10 days after RME. qRT-PCR was used to evaluate expression of Tnfsf11 (RANKL), Tnfrsf11a (RANK) and Tnfrsf11b (OPG). Data were submitted to statistical analysis using two-way ANOVA followed by Tukey test (a=0.05). There was an upregulation of RANK and RANKL genes at 7 and 10 days and an upregulation of the OPG gene at 3 and 7 days of healing. Interestingly, an increased in expression of all genes was observed over time in both RME and C groups. The RANKL/OPG ratio showed an increased signaling favoring bone resorption on RME compared to C at 3 and 7 days. Signaling against bone resorption was observed, as well as an upregulation of OPG gene expression in RME group, compared to C group at 10 days. The results of this study concluded that the RANK, RANK-L and OPG system participates in bone remodeling after RME.


Resumo O objetivo deste estudo foi avaliar a sinalização osteoclastogenese na sutura palatina após a expansão rápida da maxila (ERM) em ratos. Um total de 30 ratos Wistar machos foram divididos aleatoriamente em dois grupos com 15 animais cada: controle (C) e grupo ERM. ERM foi realizada através da inserção de um anel de metal circular de 1,5 mm de espessura entre os incisivos superiores. Os animais foram sacrificados aos 3, 7 e 10 dias após a RME. qRT-PCR foi utilizado para avaliar a expressão de Tnfsf11 (RANKL), Tnfrsf11a (RANK) e TNFRSF11b (OPG). Os dados foram submetidos à análise de variância de duas vias, seguido pelo teste de Tukey (a=0,05). Houve uma regulação positiva de genes RANK e RANKL aos 7 e 10 dias e uma regulação positiva do gene OPG aos 3 e 7 dias de tratamento. Curiosamente, foi observado um aumento na expressão de todos os genes ao longo do tempo nos grupos ERM e C. O RANKL/OPG mostrou um aumento na sinalização favorecendo a reabsorção óssea no ERM em comparação com o C nos períodos de 3 e 7 dias. Foi observada uma sinalização contra a reabsorção óssea, assim como, uma regulação favorável da expressão do gene OPG no grupo ERM, comparado ao grupo C aos 10 dias. Os resultados deste estudo permitem concluir que o sistema RANK, RANK-L e OPG participa de remodelação óssea após a ERM.


Assuntos
Animais , Masculino , Maxila/cirurgia , Osteogênese , Osteoprotegerina/genética , Técnica de Expansão Palatina/instrumentação , Ligante RANK/genética , Receptor Ativador de Fator Nuclear kappa-B/genética , Remodelação Óssea , Expressão Gênica , Maxila/metabolismo , Ratos Wistar , Reação em Cadeia da Polimerase em Tempo Real , Transdução de Sinais , Regulação para Cima , Cicatrização
7.
Braz Dent J ; 28(3): 296-300, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-29297549

RESUMO

The aim of this study was to evaluate osteoclastogenesis signaling in midpalatal suture after rapid maxillary expansion (RME) in rats. Thirty male Wistar rats were randomly assigned to two groups with 15 animals each: control (C) and RME group. RME was performed by inserting a 1.5-mm-thick circular metal ring between the maxillary incisors. The animals were euthanized at 3, 7 and 10 days after RME. qRT-PCR was used to evaluate expression of Tnfsf11 (RANKL), Tnfrsf11a (RANK) and Tnfrsf11b (OPG). Data were submitted to statistical analysis using two-way ANOVA followed by Tukey test (a=0.05). There was an upregulation of RANK and RANKL genes at 7 and 10 days and an upregulation of the OPG gene at 3 and 7 days of healing. Interestingly, an increased in expression of all genes was observed over time in both RME and C groups. The RANKL/OPG ratio showed an increased signaling favoring bone resorption on RME compared to C at 3 and 7 days. Signaling against bone resorption was observed, as well as an upregulation of OPG gene expression in RME group, compared to C group at 10 days. The results of this study concluded that the RANK, RANK-L and OPG system participates in bone remodeling after RME.


Assuntos
Maxila/cirurgia , Osteogênese , Osteoprotegerina/genética , Técnica de Expansão Palatina/instrumentação , Ligante RANK/genética , Receptor Ativador de Fator Nuclear kappa-B/genética , Animais , Remodelação Óssea , Expressão Gênica , Masculino , Maxila/metabolismo , Ratos Wistar , Reação em Cadeia da Polimerase em Tempo Real , Transdução de Sinais , Regulação para Cima , Cicatrização
8.
Dent Traumatol ; 27(2): 135-42, 2011 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-21385315

RESUMO

BACKGROUND/AIM: The aim of this retrospective study was to evaluate the epidemiology, treatment, and complications of zygomatico-orbital complex (ZOC) and/or zygomatic arch (ZA) fractures either associated with other facial fractures or not over a 71-month period. MATERIAL AND METHODS: This survey was performed in three hospitals of Ribeirao Preto in Sao Paulo, Brazil, from August 2002 to July 2008. The records of 1575 patients with facial trauma were reviewed. There were 140 cases of ZOC and ZA fractures either associated with other facial fractures or not. Data regarding gender, age, race, addictions, day of trauma, etiology, signs and symptoms, oral hygiene condition, day of initial evaluation, hospital admission, day of surgery, surgery approach, pattern of fractures, treatment performed, post-operative antibiotic therapy, day of hospital discharge, and post-operative complications were collected. The data were subjected to descriptive statistical analyses. RESULTS: The most frequent fractures affected Caucasian men and occurred during the fourth decade of life. The most frequent etiology was traffic accident, and symptoms and signs included pain and edema. Type I fractures were the main injury observed, and the treatment of choice was always rigid internal fixation. Post-operative antibiotic therapy was solely employed when there was an indication. Complications were observed in 13.1% of the cases. CONCLUSIONS: The treatment protocol yielded suitable post-operative results and also showed success rates comparable to published data around the world.


Assuntos
Fraturas Orbitárias/epidemiologia , Fraturas Zigomáticas/epidemiologia , Acidentes de Trânsito/estatística & dados numéricos , Adolescente , Adulto , Fatores Etários , Idoso , Idoso de 80 Anos ou mais , Consumo de Bebidas Alcoólicas/epidemiologia , Antibacterianos/uso terapêutico , Brasil/epidemiologia , Criança , Grupos Étnicos/estatística & dados numéricos , Feminino , Seguimentos , Fixação Interna de Fraturas/estatística & dados numéricos , Humanos , Masculino , Pessoa de Meia-Idade , Admissão do Paciente/estatística & dados numéricos , Alta do Paciente/estatística & dados numéricos , Complicações Pós-Operatórias/epidemiologia , Estudos Retrospectivos , Fatores Sexuais , Fumar/epidemiologia , Resultado do Tratamento , Violência/estatística & dados numéricos , Adulto Jovem
9.
RPG rev. pos-grad ; 18(1): 52-56, Jan.-Mar. 2011. tab
Artigo em Inglês | LILACS | ID: lil-679738

RESUMO

Scientific journals represent the main disclosure tools for the advancement and development of Science. The importance of scientific journals can be usually defined in several ways including its prestige, which may be reflected in its citedness as measured by the impact factor (IF), which is a classic parameter useful to researchers. Due to controversial issues involving scientific publication, the purpose of this update article was to clarify and explain what is the impact factor of a journal and how it can guide faculty advisors and postgraduate students to publish their papers. The IF of a journal describes both journal and author impacts. It is based on two elements: the numerator, which is the number of citations in the current year to any items published in a journal in the previous two years, and the denominator, which is the number of substantive articles (source items) published in the same two years. Although IF is the only measure of journalÆs quality, it is important to remember that the calculation of the IF is biased by many factors. These include factors as inclusion of review articles, commentaries, errata and letters in numerator, but not in the denominator of the equation used to calculate the IF. Impact factor is the foremost tool to measure and indirectly indicate the research quality. Although it is not a perfect method and has several limited interpretation power, it remains a valuable measurement technique for scientific evaluation.


Assuntos
Bases de Dados de Citações , Bases de Dados Bibliográficas , Fator de Impacto , Fator de Impacto de Revistas
10.
Pediatria (Säo Paulo) ; 33(2): 107-116, 2011. tab
Artigo em Português | LILACS | ID: lil-610177

RESUMO

Objetivo: Apresentar uma revisão da literatura e descrever os diferentes métodos de sedação consciente utilizados para o tratamento odontológico em pacientes com necessidades especiais e odontopediátricos, dando ênfase na terapia com benzodiazepínicos e na inalação da mistura dos gases óxido nitroso e oxigênio. Fontes Pesquisadas: Livre escolha de artigos científicos e livros relacionados à sedação consciente. Síntese dos Dados: Existem diversas técnicas usadas como recursos alternativos à anestesia geral para o controle e manejo da ansiedade, da dor, do medo e do comportamento em pacientes odontopediátricos e pacientes especiais. Estes métodos incluem modalidades do tipo psicológicas (não-farmacológicas) e farmacológicas. Contudo, em alguns pacientes os métodos não farmacológicos ainda não são suficientes para conseguir a compreensão e a cooperação desejada para possibilitar a realização do tratamento odontológico. Desta maneira, o cirurgião-dentista pode utilizar diversos recursos farmacológicos, como agentes sedativos, analgésicos e outros fármacos úteis para sedação destes pacientes. Dentre os métodos farmacológicos conhecidos de sedação consciente, os mais empregados em Odontologia são os que utilizam os benzodiazepínicos por via oral e a técnica inalatória por meio da mistura de óxido nitroso e oxigênio. Conclusões: A sedação consciente deve ser utilizada para procedimentos odontológicos específicos e situações em que o paciente se beneficie da sedação consciente como método farmacológico de abordagem no controle da ansiedade, da dor e do medo.


Objective: To perform a literature review and describe the several methods of conscious sedation used for the dental treatment in patients with special needs and children with emphasis in benzodiazepines therapy and the mixture of nitrous oxide and oxygen gases. Data Source: Free choice of pertinent literature scientific articles and books related to conscious sedation. Data Synthesis: There are several techniques as alternative resources to the general anesthesia for the control and handling of the anxiety, pain, fear and the behavior in children and patients with special needs. These methods include psychological (non-pharmacological) and pharmacological approaches. However, in several subjects, the psychological methods are not still enough to achieve the desirable cooperation and understanding to perform dental treatment. In this way, the dentist has available several pharmacological resources such as sedative, analgesic agents and other useful drugs for the handling and control of these patients. Among the most known pharmacological methods of conscious sedation, the most used in Dentistry are the oral benzodiazepines and the mixture of nitrous oxide and oxygen gases. Conclusions: Conscious sedation should be used for dental procedures and specific situations in which patients will benefit from conscious sedation as a method of approach in the pharmacological control of anxiety, pain and fear.


Assuntos
Humanos , Masculino , Feminino , Criança , Analgesia , Criança , Sedação Consciente , Odontopediatria
11.
Rev. odontol. Univ. Cid. Sao Paulo ; 22(2): 138-146, maio-ago. 2010.
Artigo em Português | LILACS, BBO - Odontologia | ID: lil-563880

RESUMO

O objetivo deste trabalho é descrever as características clínicas, radiográficas e histológicas, assim como aspectos relacionados ao tratamento e prognóstico das patologias tumoriformes não odontogênicas mais comumente encontradas na cavidade bucal de crianças. Dentre elas estão o papiloma, o hemangioma, o linfangioma, a epúlide congênita do recém-nascido, o granuloma piogênico, as lesões periféricas e centrais de células gigantes e o fibroma ossificante periférico.


The aim of this review is to relate the clinical, radiographical and histological aspects, the treatment andprognostic of non odontogenic tumors commonly found in the oral cavity of children. Beside these tumors are the papilloma, hemangioma, lymphangioma, neonatal congenital epulis, pyogenic granuloma, peripheral and central giant cell lesions, and peripheral ossifying fibroma.


Assuntos
Humanos , Masculino , Feminino , Criança , Doenças da Gengiva/diagnóstico , Hemangioma/diagnóstico , Linfangioma/diagnóstico , Neoplasias Bucais/diagnóstico , Papiloma/diagnóstico
12.
Clin Oral Implants Res ; 20(11): 1254-64, 2009 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-19531103

RESUMO

OBJECTIVES: Little information is available on the molecular events that occur during graft incorporation over time. The calvarial bone (Cb) grafts have been reported to produce greater responses compared with other donor regions in maxillofacial reconstructions, but the scientific evidences for this are still lacking. The objectives of this study are (1) to study the morphological pattern of Cb onlay bone grafts and compare them with the biological events through immunohistochemical responses and (2) to establish the effects of perforations in maintaining the volume and bone density of the receptor bed. MATERIAL AND METHODS: Sixty New Zealand White rabbits were submitted to Cb onlay bone grafts on the mandible. In 30 rabbits, the receptor bed was perforated (perforated group), while for the remaining animals the bed was kept intact (non-perforated group). Six animals from each group were sacrificed at 5, 7, 10, 20 and 60 days after surgery. Histological sections from the grafted area were prepared for immunohistochemical and histological analyses. Immuno-labeling was found for proteins Osteoprotegerin (OPG), receptor activator of nuclear factor-kappabeta ligand (RANKL), alkaline phosphatase (ALP), osteopontin (OPN), vascular endothelial growth factor (VEGF), tartrate-resistant acid phosphatase (TRAP), Type I collagen (COL I) and osteocalcin (OC). The tomography examination [computerized tomography (CT) scan] was conducted just after surgery and at the sacrifice. RESULTS: The histological findings revealed that the perforations contributed to higher bone deposition during the initial stages at the graft-receptor bed interface, accelerating the graft incorporation process. The results of the CT scan showed lower resorption for the perforated group (P

Assuntos
Transplante Ósseo/fisiologia , Sobrevivência de Enxerto/fisiologia , Osseointegração/fisiologia , Osteogênese/fisiologia , Crânio/cirurgia , Fosfatase Ácida/fisiologia , Fosfatase Alcalina/fisiologia , Animais , Reabsorção Óssea/metabolismo , Transplante Ósseo/métodos , Colágeno Tipo I/fisiologia , Imuno-Histoquímica , Isoenzimas/fisiologia , Masculino , Osteocalcina/fisiologia , Osteopontina/fisiologia , Osteoprotegerina/fisiologia , Ligante RANK/fisiologia , Coelhos , Estatísticas não Paramétricas , Fosfatase Ácida Resistente a Tartarato , Tomografia Computadorizada por Raios X , Fator A de Crescimento do Endotélio Vascular/fisiologia
13.
J. Health Sci. Inst ; 23(2): 163-168, abr.-jun. 2005. ilus, CD-ROM
Artigo em Português | LILACS, BBO - Odontologia | ID: biblio-872876

RESUMO

O odontoma composto é um tumor em crianças e adolescentes. Neste trabalho, após a aprovação do Comitê de Ética em Pesquisa da Faculdade de Odontologia de Ribeirão Preto da Universidade de São Paulo, é relatado o caso clínico de um paciente com 7 anos e 5 meses de idade que compareceu à Clínica de Odontopediatria desta mesma Faculdade, com queixa principal de retenção prolongada do incisivo central superior decíduo direito. Durante o exame radiográfico observou-se uma imagem radiopaca irregular, entre as raízes dos incisivos central e lateral superiores decíduos do lado direito. O exame histopatológico confirmou que se tratava de um odontoma composto. É apresentada breve revisão da literatura sobre a classificação, os aspectos etiológicos, clínicos, radiográficos e histológicos, bem como o diagnóstico diferencial e o tratamento desta lesão.


Assuntos
Humanos , Masculino , Criança , Dente Decíduo , Odontoma/diagnóstico , Tumores Odontogênicos , Erupção Dentária , Estética Dentária , Odontoma/cirurgia , Odontopediatria , Osteotomia
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