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1.
Braz. j. oral sci ; 20: e219342, jan.-dez. 2021. ilus
Artigo em Inglês | LILACS, BBO - Odontologia | ID: biblio-1253927

RESUMO

Aim: The aim of this study was to compare the microtensile bond strength (µTBS) and the characteristics of the adhesive interface of Scotchbond Universal - SU ­ etch-and-rise mode (3M ESPE) and Adper Scotchbond Multi-Purpose - MP (3M ESPE) to dentin over time. Methods: Class I cavity preparations were performed in 60 human molars that were randomly divided according to the dentin bonding system (DBS) used (n=30): (1) Acid conditioning + SU and (2) Acid conditioning + MP. For bonding strength (BS) analysis, 30 teeth (n = 15) were sectioned into sticks and submitted to the microtensile test in a universal testing machine after 24 hours and 12 months. The adhesive interface of the others 30 teeth was analyzed in a confocal microscope after 24 hours and 12 months. The data of µTBS were analyzed by two-way repeated measures ANOVA and Tukey's HSD (α = 0.05). Results: SU presented the lowest DBS compared to MP (p=0.000). Time did not influenced DBS for both adhesive systems (p=0.177). Confocal microscopy analysis showed no cracks between both adhesive systems tested. Conclusion: The results indicate that MP - µTBS showed a better performance compared to SU in total-etch mode


Assuntos
Humanos , Adesivos Dentinários , Microscopia Confocal , Dentina , Metacrilatos
2.
Braz Dent J ; 31(3): 244-251, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32667520

RESUMO

This in vitro study evaluated cell viability and metabolism, nitric oxide release and production of two chemokines and one cytokine by cultured human dental pulp fibroblasts (HDPF) in contact with two glass ionomer cements (Ketac Molar-KM and Vitrebond-VB), Single Bond (SB) and calcium hydroxide (Dycal-DY). Cultures of HDPF were established by means of an explant technique. The specimens were prepared under sterile conditions and in disks measuring 5 mm x 2 mm obtained from a prefabricated mold and placed on a permeable membrane to avoid direct contact with the cells. Cytotoxicity was assessed by Trypan Blue exclusion method and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Nitric oxide release in cell supernatant was detected by the Griess Method whereas stromal derived factor-1 alpha (SDF-1α or CXCL12), chemokine (C-X-C motif) ligand 8 [Interleukin 8 (IL-8 or CXCL8)] and interleukin-6 (IL-6) were detected by ELISA. RT-qPCR was employed for gene expression analysis. Statistical analyses were performed by One-way ANOVA followed by Tukey's post hoc test for materials independent of the time, and Two-way ANOVA followed by Bonferroni correction test for the comparisons between materials and experimental time (p<0.05). Cytotoxic tests showed significant differences only for DY. Protein levels and mRNA expression were significantly increased for IL-8 for both periods of time. IL-6 production increased when fibroblasts were stimulated by KM. SDF-1α protein production and mRNA expression were not affected by any of the materials. There was a decrease in nitrate/nitrite levels only for KM. Although DY caused intense cell death and did not stimulate the production of the inflammatory mediators evaluated in this work, it is known that this event seems to be fundamental for the process of repair of the pulp tissue and formation of mineralized barrier. KM and VB increased production of proteins related to the inflammatory process, thus favoring tissue repair. Therefore, although these glass ionomer cements did not lead to large cell death, they should be used with caution.


Assuntos
Capeamento da Polpa Dentária , Polpa Dentária , Fibroblastos , Humanos
3.
Braz. dent. j ; 31(3): 244-251, May-June 2020. tab, graf
Artigo em Inglês | LILACS-Express | LILACS, BBO - Odontologia | ID: biblio-1132303

RESUMO

Abstract This in vitro study evaluated cell viability and metabolism, nitric oxide release and production of two chemokines and one cytokine by cultured human dental pulp fibroblasts (HDPF) in contact with two glass ionomer cements (Ketac Molar-KM and Vitrebond-VB), Single Bond (SB) and calcium hydroxide (Dycal-DY). Cultures of HDPF were established by means of an explant technique. The specimens were prepared under sterile conditions and in disks measuring 5 mm x 2 mm obtained from a prefabricated mold and placed on a permeable membrane to avoid direct contact with the cells. Cytotoxicity was assessed by Trypan Blue exclusion method and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Nitric oxide release in cell supernatant was detected by the Griess Method whereas stromal derived factor-1 alpha (SDF-1α or CXCL12), chemokine (C-X-C motif) ligand 8 [Interleukin 8 (IL-8 or CXCL8)] and interleukin-6 (IL-6) were detected by ELISA. RT-qPCR was employed for gene expression analysis. Statistical analyses were performed by One-way ANOVA followed by Tukey's post hoc test for materials independent of the time, and Two-way ANOVA followed by Bonferroni correction test for the comparisons between materials and experimental time (p<0.05). Cytotoxic tests showed significant differences only for DY. Protein levels and mRNA expression were significantly increased for IL-8 for both periods of time. IL-6 production increased when fibroblasts were stimulated by KM. SDF-1α protein production and mRNA expression were not affected by any of the materials. There was a decrease in nitrate/nitrite levels only for KM. Although DY caused intense cell death and did not stimulate the production of the inflammatory mediators evaluated in this work, it is known that this event seems to be fundamental for the process of repair of the pulp tissue and formation of mineralized barrier. KM and VB increased production of proteins related to the inflammatory process, thus favoring tissue repair. Therefore, although these glass ionomer cements did not lead to large cell death, they should be used with caution.


Resumo Este estudo avalia in vitro a viabilidade e metabolismo celular, a liberação de óxido nítrico e a produção de duas quimiocinas e uma citocina por fibroblastos de polpa dentária humana em cultura (FPDH) em contato com dois cimentos de ionômero de vidro (Ketac Molar-KM e Vitrebond-VB), Single Bond (SB) e hidróxido de cálcio (Dycal-DY). As culturas de FPDH foram estabelecidas por meio de uma técnica de explante. As amostras foram preparadas em condições estéreis e em discos de 5 mm x 2 mm, obtidas de um molde pré-fabricado e colocadas em uma membrana permeável (Maxicell 24 W 0,4 µm) para evitar o contato direto com as células. A citotoxicidade foi avaliada pelo método de exclusão de Trypan Blue e pelo ensaio de MTT. A liberação de óxido nítrico no sobrenadante celular foi detectada pelo método Griess, enquanto fator 1 derivado do estroma (SDF-1α ou CXCL12), interleucina-8 (IL-8 ou CXCL8) and interleucina-6 (IL-6) foram detectados por ELISA. RT-qPCR foi empregada para análise de expressão gênica. As análises estatísticas foram realizadas por ANOVA a 1 critério, seguida pelo pós-teste de Tukey para os materiais independentes do tempo, e ANOVA a 2 critérios, seguida pelo teste de correção de Bonferroni para comparações entre materiais e tempo experimental (p<0,05). Os testes citotóxicos mostraram diferenças significativas apenas para DY. Os níveis da proteína e a expressão de RNAm para IL-8 aumentaram significativamente para ambos os tempos estudados. A produção de IL-6 aumentou quando os fibroblastos foram estimulados por KM. A produção da proteína e a expressão de RNAm para SDF-1α não foram afetadas por nenhum dos materiais. Houve uma diminuição nos níveis de nitrato/nitrito apenas para KM. Embora o DY tenha causado intensa morte celular e não tenha estimulado a produção dos mediadores inflamatórios avaliados neste trabalho, sabe-se que esse evento parece ser fundamental para o processo de reparo do tecido pulpar e formação de barreira mineralizada. Os cimentos de ionômero de vidro utilizados aumentaram a produção de proteínas relacionadas ao processo inflamatório, favorecendo a reparação tecidual e, portanto, esses materiais, embora não causem grande morte celular, devem ser utilizados com cautela.

4.
Gen Dent ; 67(5): 24-28, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31454318

RESUMO

Dental caries is a significant oral health issue and public health concern. Currently conceptualized as a dynamic and multifactorial process, caries is a continuum of disease stages, during which tooth damage may be reversed or controlled, depending on lesion progression. The International Caries Detection and Assessment System, when used in conjunction with the Lesion Activity Assessment (ICDAS-LAA), reflects the dynamic caries process and allows the classification of cavitated lesions according to their severity and progression, which is essential to selecting the appropriate treatment. This case report describes the diagnosis and treatment of a patient who had caries in the past and in whom old lesions remained untreated. The ICDAS-LAA was used to assess caries accurately and guide treatment decisions. The patient maintained a dietary journal for 2 weeks to help establish her risk of developing caries. Treatment involved a minimally invasive dentistry approach, which consisted of remineralization via fluoride applications, placement of a composite resin restoration, microabrasion, and periodic maintenance. During the 28-month follow-up period, rigorous control of caries risk factors was achieved, and the results were satisfactory, demonstrating the effectiveness of the ICDAS-LAA in diagnosing and managing carious lesions.


Assuntos
Assistência Odontológica/tendências , Cárie Dentária , Cárie Dentária/diagnóstico , Cárie Dentária/terapia , Feminino , Seguimentos , Humanos , Fatores de Risco , Dente
5.
Gen Dent ; 67(2): e7-e10, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30875313

RESUMO

The purpose of this in vitro study was to evaluate the influence of depth (3.0 vs 5.0 mm) of mesio-occlusodistal (MOD) cavity preparation and mechanical cycling on fracture resistance of maxillary premolars restored with resin-based composite (RBC). Seventy premolars were randomly divided into 7 groups (n = 10): NP, no cavity preparation (control); MOD3, 3.0-mm-deep MOD preparation, not restored; MOD3R, 3.0-mm-deep MOD preparation restored with RBC; MOD5, 5.0-mm-deep MOD preparation, not restored; MOD5R, 5.0-mm-deep MOD preparation restored with RBC; MOD3RC, 3.0-mm-deep MOD preparation restored with RBC and subjected to mechanical cycling; and MOD5RC, 5.0-mm-deep MOD preparation restored with RBC and subjected to mechanical cycling under the same conditions as MOD3RC. All specimens were tested using a universal testing machine, resulting in the following mean (SD) values: NP, 224.65 (35.66) kgf; MOD3, 58.92 (15.48) kgf; MOD3R, 95.58 (13.88) kgf; MOD5, 68.06 (10.12) kgf; MOD5R, 98.49 (13.16) kgf; MOD3RC, 83.68 (14.39) kgf; and MOD5RC, 54.25 (11.56) kgf. Analysis of variance and Tukey test showed no statistically significant difference among MOD3, MOD5, and MOD5RC; between MOD5 and MOD3RC; or among MOD3R, MOD5R, and MOD3RC (P > 0.05). MOD3RC had significantly greater fracture resistance than MOD5RC (P < 0.05). NP had the highest fracture resistance of all groups (P < 0.05). The results showed that the depth of the cavity preparation only influenced fracture resistance after mechanical cycling of the specimens.


Assuntos
Preparo da Cavidade Dentária , Restauração Dentária Permanente , Fraturas dos Dentes , Dente não Vital , Dente Pré-Molar , Resinas Compostas , Preparo da Cavidade Dentária/métodos , Restauração Dentária Permanente/métodos , Análise do Estresse Dentário , Humanos , Técnicas In Vitro , Estresse Mecânico
6.
Braz Dent J ; 29(5): 419-426, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30517439

RESUMO

This study evaluated in vitro cell viability and metabolism, nitric oxide release and production of chemokines by cultured human dental pulp fibroblasts (DPF) under contact with HEMA and Single Bond. Cultures of DPF were established by means of an explant technique. Once plated, cells were kept under contact with increasing concentrations of HEMA (10, 100 and 1000 nM) or Single Bond (SB) [10-fold serially diluted in culture medium (10-4, 10-3 and 10-2 v/v)] and also with polymerized SB components. Cytotoxicity was assessed by Trypan Blue exclusion method and MTT [3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay. Nitric oxide release on cell supernatant was detected by Griess Method whereas chemokines (CXCL12 and CXCL8) were detected by ELISA. RT-qPCR was employed for chemokines gene expression analysis. Cytotoxic tests showed significant differences for SB 10-2. None of the tested materials significantly altered NO levels. Protein levels of CXCL12 were significantly decreased only by HEMA. On the other hand, while CXCL12 mRNA remained unaltered, gene expression of CXCL8 had significant decrease with all materials, except for polymerized SB. In conclusion, Single Bond and HEMA at various concentrations, decreased expression and production of molecules involved in inflammatory processes and, therefore, the use of adhesive systems such as pulp capping materials must be viewed with caution due to its large cytotoxic effect when in close contact with the pulp.


Assuntos
Bis-Fenol A-Glicidil Metacrilato/farmacologia , Polpa Dentária/citologia , Fibroblastos/efeitos dos fármacos , Metacrilatos/farmacologia , Sobrevivência Celular , Células Cultivadas , Quimiocinas/metabolismo , Humanos , Técnicas In Vitro , Teste de Materiais , Óxido Nítrico/metabolismo , Reação em Cadeia da Polimerase
7.
Braz. dent. j ; 29(5): 419-426, Sept.-Oct. 2018. graf
Artigo em Inglês | LILACS-Express | LILACS | ID: biblio-974185

RESUMO

Abstract This study evaluated in vitro cell viability and metabolism, nitric oxide release and production of chemokines by cultured human dental pulp fibroblasts (DPF) under contact with HEMA and Single Bond. Cultures of DPF were established by means of an explant technique. Once plated, cells were kept under contact with increasing concentrations of HEMA (10, 100 and 1000 nM) or Single Bond (SB) [10-fold serially diluted in culture medium (10-4, 10-3 and 10-2 v/v)] and also with polymerized SB components. Cytotoxicity was assessed by Trypan Blue exclusion method and MTT [3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay. Nitric oxide release on cell supernatant was detected by Griess Method whereas chemokines (CXCL12 and CXCL8) were detected by ELISA. RT-qPCR was employed for chemokines gene expression analysis. Cytotoxic tests showed significant differences for SB 10-2. None of the tested materials significantly altered NO levels. Protein levels of CXCL12 were significantly decreased only by HEMA. On the other hand, while CXCL12 mRNA remained unaltered, gene expression of CXCL8 had significant decrease with all materials, except for polymerized SB. In conclusion, Single Bond and HEMA at various concentrations, decreased expression and production of molecules involved in inflammatory processes and, therefore, the use of adhesive systems such as pulp capping materials must be viewed with caution due to its large cytotoxic effect when in close contact with the pulp.


Resumo Este estudo avaliou in vitro a viabilidade e metabolismo celular, liberação de óxido nítrico e produção de quimiocinas em cultura de fibroblastos de polpa dental humana (DPF) em contato com HEMA e Single Bond. Culturas de DPF foram estabelecidas por meio de uma técnica de explante. Uma vez plaqueadas, as células foram mantidas em contato com concentrações crescentes de HEMA (10, 100 e 1000 nM) ou Single Bond (SB) [10 vezes diluídas em série em meio de cultura (10-4, 10-3 e 10-2 v/v)] e também com SB polimerizado. A citotoxicidade foi avaliada pelo método de exclusão de Trypan Blue e pelo ensaio de 3-(4,5-dimetiltiazol-2-il)-2,5-difeniltetrazólio brometo (MTT). A liberação de óxido nítrico no sobrenadante celular foi detectada pelo método de Griess, enquanto as quimiocinas (CXCL12 e CXCL8) foram detectadas por ELISA. RT-qPCR foi empregada para análise de expressão gênica de quimiocinas. Testes citotóxicos mostraram diferenças significativas para SB 10-2. Nenhum dos materiais testados alterou significativamente os níveis de NO. Os níveis de proteína de CXCL12 foram significativamente diminuídos apenas pelo HEMA. Por outro lado, enquanto o RNAm de CXCL12 permaneceu inalterado, a expressão gênica de CXCL8 teve redução significativa com todos os materiais, com exceção do SB polimerizado. Em conclusão, Single Bond e HEMA, em várias concentrações, diminuíram a expressão e produção de moléculas envolvidas em processos inflamatórios e, portanto, o uso de sistemas adesivos, como o material protetor da polpa, deve ser visto com cautela devido ao seu grande efeito citotóxico quando em contato com a polpa.

8.
Braz Dent J ; 27(5): 548-555, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27982232

RESUMO

This study evaluated the impact of orange juice on the bond strength (BS) of dentin bonding systems (DBSs) to enamel surface after simulation with an in situ/ ex vivo erosive cycling. One hundred and ninety two bovine enamel fragments (4x4x2mm) were obtained and randomized regarding superficial microhardness and distributed to palatal devices for 8 volunteers, in three phases (one for each DBS), containing 8 blocks, which were, allocated in 4 pairs. Daily, these pairs were subjected extraorally to the following conditions: CONT- neither erosive nor abrasive challenge; ERO- erosive challenge only; ABR- abrasive challenge only and ERO + ABR- with erosive and abrasive challenges. Erosive cycles (immersion in orange juice, 3 times/day/5 min/5 days) or/and abrasive challenges (electric toothbrush, 3 times/day/1 min/5 days) were performed. After these cycles, all specimens were restored with the adhesive systems Adper Scotchbond Multi Purpose (MP), Adper Single Bond 2 (SB) or Clearfil SE Bond (SE), and the composite resin Filtek Z250. After 7 days, sticks (area ≅1 mm2) were obtained and subjected to the microtensile bond strength test (µTBS) at 0.5 mm/min. Data was statistically analyzed by ANOVA and Tukey tests (a=0.05). Failure modes were determined using a digital microscope (40´). DBS was the only statistical significant factor. SE was the unique DBS not affected in any challenge, whereas MP and SB performed according to the scenario. The adhesive and mixed failures were predominant in all groups. Overall performance suggested that BS to enamel after erosive /abrasive challenged by orange juice was not affected and it was material-dependent.


Assuntos
Colagem Dentária , Animais , Bovinos , Resistência à Tração
9.
Braz. dent. j ; 27(5): 548-555, Sept.-Oct. 2016. tab, graf
Artigo em Inglês | LILACS | ID: biblio-828034

RESUMO

Abstract This study evaluated the impact of orange juice on the bond strength (BS) of dentin bonding systems (DBSs) to enamel surface after simulation with an in situ/ ex vivo erosive cycling. One hundred and ninety two bovine enamel fragments (4x4x2mm) were obtained and randomized regarding superficial microhardness and distributed to palatal devices for 8 volunteers, in three phases (one for each DBS), containing 8 blocks, which were, allocated in 4 pairs. Daily, these pairs were subjected extraorally to the following conditions: CONT- neither erosive nor abrasive challenge; ERO- erosive challenge only; ABR- abrasive challenge only and ERO + ABR- with erosive and abrasive challenges. Erosive cycles (immersion in orange juice, 3 times/day/5 min/5 days) or/and abrasive challenges (electric toothbrush, 3 times/day/1 min/5 days) were performed. After these cycles, all specimens were restored with the adhesive systems Adper Scotchbond Multi Purpose (MP), Adper Single Bond 2 (SB) or Clearfil SE Bond (SE), and the composite resin Filtek Z250. After 7 days, sticks (area ≅1 mm2) were obtained and subjected to the microtensile bond strength test (μTBS) at 0.5 mm/min. Data was statistically analyzed by ANOVA and Tukey tests (a=0.05). Failure modes were determined using a digital microscope (40´). DBS was the only statistical significant factor. SE was the unique DBS not affected in any challenge, whereas MP and SB performed according to the scenario. The adhesive and mixed failures were predominant in all groups. Overall performance suggested that BS to enamel after erosive /abrasive challenged by orange juice was not affected and it was material-dependent.


Resumo Este estudo avaliou o impacto de suco de laranja na resistência de união (RU) de sistemas adesivos dentinários (SAD) à superfície do esmalte após a simulação com uma ciclagem erosiva in situ/ex vivo. Cento e noventa e dois fragmentos de esmalte bovino (4x4x2mm) foram obtidos e randomizados considerando a microdureza superficial, e distribuídos em dispositivos palatinos para 8 voluntários, em três fases (uma para cada SAD), contendo 8 blocos, os quais foram alocados em 4 pares. Diariamente, esses pares eram submetidos às seguintes condições extraoralmente: CONT- sem desafio erosivo ou abrasivo; ERO- desafio erosivo somente; ABR- desafio abrasivo somente; e ERO+ABR- com desafio erosivo e abrasivo. A ciclagem erosiva (imersão em suco de laranja, 3 vezes/dia/5 min/5 dias) e/ou ciclagem abrasiva (escova dentária elétrica, 3 vezes/dia/1 min/5 dias) foram feitas. Após estas ciclagens, todos os espécimes foram restaurados com os sistemas adesivos Adper Scotchbond Multi Purpose (MP), Adper Single Bond 2 (SB) ou Clearfil SE Bond (SE), e com a resina composta Filtek Z250. Após 7 dias, palitos (área ≈1 mm) eram obtidos e submetidos ao teste de resistência de união por microtração (μTBS) a 0,5 mm/min. Os dados foram estatisticamente analisados por ANOVA e teste de Tukey (a=0,05). Os modos de fratura foram determinados utilizando um microscópio digital (40×). SAD foi o único fator estatisticamente significante. SE foi o único SAD não afetado por qualquer desafio, enquanto o MP e o SB apresentaram um desempenho de acordo com o cenário. As fraturas do tipo mista e adesiva foram predominantes em todos os grupos. O desempenho geral sugeriu que RU ao esmalte após desafio erosivo/abrasivo por suco de laranja não foi afetada e foi material-dependente.


Assuntos
Animais , Bovinos , Colagem Dentária , Resistência à Tração
10.
J Appl Oral Sci ; 24(4): 317-24, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27556201

RESUMO

OBJECTIVE: This study investigated the effect of the fluorescent dye rhodamine B (RB) for interfacial micromorphology analysis of dental composite restorations on water sorption/solubility (WS/WSL) and microtensile bond strength to dentin (µTBS) of a 3-step total etch and a 2-step self-etch adhesive system. MATERIAL AND METHODS: The adhesives Adper Scotchbond Multi-Purpose (MP) and Clearfil SE Bond (SE) were mixed with 0.1 mg/mL of RB. For the WS/WSL tests, cured resin disks (5.0 mm in diameter x 0.8 mm thick) were prepared and assigned into four groups (n=10): MP, MP-RB, SE, and SE-RB. For µTBS assessment, extracted human third molars (n=40) had the flat occlusal dentin prepared and assigned into the same experimental groups (n=10). After the bonding and restoration procedures, specimens were sectioned in rectangular beams, stored in water and tested after seven days or after 12 months. The failure mode of fractured specimens was qualitatively evaluated under optical microscope (x40). Data from WS/WSL and µTBS were assessed by one-way and three-way ANOVA, respectively, and Tukey's test (α=5%). RESULTS: RB increased the WSL of MP and SE. On the other hand, WS of both MP and SE was not affected by the addition of RB. No significance in µTBS between MP and MP-RB for seven days or one year was observed, whereas for SE a decrease in the µTBS means occurred in both storage times. CONCLUSIONS: RB should be incorporated into non-simplified DBSs with caution, as it can interfere with their physical-mechanical properties, leading to a possible misinterpretation of bonded interface.


Assuntos
Adesivos Dentinários/química , Dentina/efeitos dos fármacos , Corantes Fluorescentes/química , Rodaminas/química , Água/química , Resinas Compostas/química , Colagem Dentária/métodos , Falha de Restauração Dentária , Humanos , Teste de Materiais , Microscopia Confocal , Reprodutibilidade dos Testes , Cimentos de Resina/química , Solubilidade , Propriedades de Superfície , Resistência à Tração , Fatores de Tempo
11.
J. appl. oral sci ; 24(4): 317-324, July-Aug. 2016. tab, graf
Artigo em Inglês | LILACS | ID: lil-792589

RESUMO

ABSTRACT Objective This study investigated the effect of the fluorescent dye rhodamine B (RB) for interfacial micromorphology analysis of dental composite restorations on water sorption/solubility (WS/WSL) and microtensile bond strength to dentin (µTBS) of a 3-step total etch and a 2-step self-etch adhesive system. Material and Methods The adhesives Adper Scotchbond Multi-Purpose (MP) and Clearfil SE Bond (SE) were mixed with 0.1 mg/mL of RB. For the WS/WSL tests, cured resin disks (5.0 mm in diameter x 0.8 mm thick) were prepared and assigned into four groups (n=10): MP, MP-RB, SE, and SE-RB. For µTBS assessment, extracted human third molars (n=40) had the flat occlusal dentin prepared and assigned into the same experimental groups (n=10). After the bonding and restoration procedures, specimens were sectioned in rectangular beams, stored in water and tested after seven days or after 12 months. The failure mode of fractured specimens was qualitatively evaluated under optical microscope (x40). Data from WS/WSL and µTBS were assessed by one-way and three-way ANOVA, respectively, and Tukey’s test (α=5%). Results RB increased the WSL of MP and SE. On the other hand, WS of both MP and SE was not affected by the addition of RB. No significance in µTBS between MP and MP-RB for seven days or one year was observed, whereas for SE a decrease in the µTBS means occurred in both storage times. Conclusions RB should be incorporated into non-simplified DBSs with caution, as it can interfere with their physical-mechanical properties, leading to a possible misinterpretation of bonded interface.


Assuntos
Humanos , Rodaminas/química , Água/química , Adesivos Dentinários/química , Dentina/efeitos dos fármacos , Corantes Fluorescentes/química , Solubilidade , Propriedades de Superfície , Resistência à Tração , Fatores de Tempo , Teste de Materiais , Reprodutibilidade dos Testes , Colagem Dentária/métodos , Microscopia Confocal , Resinas Compostas/química , Cimentos de Resina/química , Falha de Restauração Dentária
12.
Braz. dent. sci ; 19(2): 121-131, 2016. ilus
Artigo em Inglês | LILACS | ID: lil-788622

RESUMO

The purpose of this clinical case description article was to present the planning sequence, indication, treatment and performance to obtain a crown of the Endocrown type. This clinical case description article illustrates a therapeutic option for functional and esthetic rehabilitation of a devitalized mandibular molar, presenting a small amount of remaining coronal tooth structure, by means of cementing a pure porcelain crown of the Endocrowntype (Adhesive Endodontic Crown). A 39-year-oldwoman presented to the Graduate clinic of the Bauru Dental School (FOB), University of São Paulo (USP),with the complaint that tooth #36 had an extensive and unsatisfactory composite resin restoration. The lithium disilicate-based system of monolithic porcelain was used with the lost wax technique. This therapy promoted the stability and retention of the indirect restoration, without the need for performing reconstruction of the above mentioned tooth, either by means of a cast metal core or reconstruction with intracanal post, there by reducing the treatment time. After the tooth preparation, the provisional restoration was done with acrylic resin to determine if the retention and stability of the remaining tooth was adequate to receive the indirect. The characteristics of the internal and external walls of the dental remnants, cervical termination, impression-taking, laboratory stages and adhesive cementation will be discussed. The major advantage of indicating an endocrown is the use of the dental remnants it self, particularly the pulp chamber, to promote retention and stability in cases without adequate height for performing complete dental and crown reconstruction...


O objetivo deste artigo de descrição de caso clínico foi apresentar a sequência de planejamento, indicação, tratamento e execução de uma coroa do tipo Endocrown (Coroa Endodôntica Adesiva).Este artigo ilustra uma opção terapêutica para reabilitação funcional e estética de um molar inferior desvitalizado, apresentando uma pequena quantidade de remanescente coronário, por meio da cimentação de uma coroa pura de porcelana do tipo Endocrown. Uma mulher de 39 anos de idade, apresentou-se à clínica de Pós-Graduação da Faculdade de Odontologia de Bauru (FOB) da Universidade de São Paulo(USP), com a queixa de uma extensa e insatisfatória restauração de resina composta no dente 36. O sistema monolítico de porcelana à base de dissilicato de lítio foi utilizado com a técnica da cera perdida.Esta técnica promoveu a estabilidade e a retenção da restauração indireta, sem a necessidade de realizara reconstrução do dente 36, quer por meio de um núcleo metálico fundido ou reconstrução com pino intra canal, reduzindo assim o tempo de tratamento.Após o preparo do dente, realizou-se a restauração provisória com resina acrílica e verificou-se se a retenção e estabilidade do remanescente dentário era adequada para receber a restauração do tipo Endocrown. As características das paredes internas e externas do preparo do remanescente dentário,término cervical, moldagem, etapas laboratoriais e de cimentação são discutidas. A principal vantagem da indicação de uma coroa do tipo Endocrown é a utilização do próprio remanescente dentário,particularmente a câmara pulpar, para promover a retenção e estabilidade em casos onde não haja altura adequada para a reconstrução coronária...


Assuntos
Humanos , Coroas , Cimentos Dentários , Dente não Vital
13.
J Appl Oral Sci ; 23(3): 315-20, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26221927

RESUMO

UNLABELLED: Interface integrity can be maintained by setting the composite in a layering technique and using liners. OBJECTIVE: The aim of this in vitro study was to verify the effect of resin-modified glass-ionomer cement (RMGIC) lining and composite layering technique on the bond strength of the dentin/resin adhesive interface of lateral walls of occlusal restorations. MATERIAL AND METHODS: Occlusal cavities were prepared in 52 extracted sound human molars, randomly assigned into 4 groups: Group 2H (control) - no lining + two horizontal layers; Group 4O: no lining + four oblique layers; Group V-2H: RMGIC lining (Vitrebond) + two horizontal layers; and Group V-4O: RMGIC lining (Vitrebond) + four oblique layers. Resin composite (Filtek Z250, 3M ESPE) was placed after application of an adhesive system (Adper™ Single Bond 2, 3M ESPE) dyed with a fluorescent reagent (Rhodamine B) to allow confocal microscopy analysis. The teeth were stored in deionized water at 37oC for 24 hours before being sectioned into 0.8 mm slices. One slice of each tooth was randomly selected for Confocal Laser Scanning Microscopy (CLSM) analysis. The other slices were sectioned into 0.8 mm x 0.8 mm sticks to microtensile bond strength test (MPa). Data were analyzed by two-way ANOVA and Fisher's test. Results There was no statistical difference on bond strength among groups (p>0.05). CLSM analysis showed no significant statistical difference regarding the presence of gap at the interface dentin/resin among groups. CONCLUSIONS: RMGIC lining and composite layering techniques showed no effect on the microtensile bond strength and gap formation at the adhesive interface of lateral walls of high C-factor occlusal restorations.


Assuntos
Resinas Compostas/química , Colagem Dentária/métodos , Adesivos Dentinários/química , Dentina/efeitos dos fármacos , Cimentos de Ionômeros de Vidro/química , Análise de Variância , Bis-Fenol A-Glicidil Metacrilato/química , Preparo da Cavidade Dentária/métodos , Falha de Restauração Dentária , Restauração Dentária Temporária/métodos , Humanos , Teste de Materiais , Microscopia Confocal , Estresse Mecânico , Propriedades de Superfície/efeitos dos fármacos , Resistência à Tração , Fatores de Tempo
14.
J. appl. oral sci ; 23(3): 315-320, May-Jun/2015. graf
Artigo em Inglês | LILACS | ID: lil-752427

RESUMO

Interface integrity can be maintained by setting the composite in a layering technique and using liners. Objective The aim of this in vitro study was to verify the effect of resin-modified glass-ionomer cement (RMGIC) lining and composite layering technique on the bond strength of the dentin/resin adhesive interface of lateral walls of occlusal restorations. Material and Methods Occlusal cavities were prepared in 52 extracted sound human molars, randomly assigned into 4 groups: Group 2H (control) – no lining + two horizontal layers; Group 4O: no lining + four oblique layers; Group V-2H: RMGIC lining (Vitrebond) + two horizontal layers; and Group V-4O: RMGIC lining (Vitrebond) + four oblique layers. Resin composite (Filtek Z250, 3M ESPE) was placed after application of an adhesive system (Adper™ Single Bond 2, 3M ESPE) dyed with a fluorescent reagent (Rhodamine B) to allow confocal microscopy analysis. The teeth were stored in deionized water at 37oC for 24 hours before being sectioned into 0.8 mm slices. One slice of each tooth was randomly selected for Confocal Laser Scanning Microscopy (CLSM) analysis. The other slices were sectioned into 0.8 mm x 0.8 mm sticks to microtensile bond strength test (MPa). Data were analyzed by two-way ANOVA and Fisher's test. Results There was no statistical difference on bond strength among groups (p>0.05). CLSM analysis showed no significant statistical difference regarding the presence of gap at the interface dentin/resin among groups. Conclusions RMGIC lining and composite layering techniques showed no effect on the microtensile bond strength and gap formation at the adhesive interface of lateral walls of high C-factor occlusal restorations. .


Assuntos
Humanos , Resinas Compostas/química , Colagem Dentária/métodos , Adesivos Dentinários/química , Dentina/efeitos dos fármacos , Cimentos de Ionômeros de Vidro/química , Análise de Variância , Bis-Fenol A-Glicidil Metacrilato/química , Falha de Restauração Dentária , Preparo da Cavidade Dentária/métodos , Restauração Dentária Temporária/métodos , Teste de Materiais , Microscopia Confocal , Estresse Mecânico , Propriedades de Superfície/efeitos dos fármacos , Resistência à Tração , Fatores de Tempo
15.
Am J Dent ; 27(3): 145-8, 2014 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-25208362

RESUMO

PURPOSE: To evaluate the effect of bleaching techniques on dentin permeability. The hypothesis was that the bleaching agent associated or not to etching and/or energy source would be able to increase dentin permeability (conductance). METHODS: Fifty 1 mm-thick disks of mid-coronal dentin were obtained from human third molars, which were mounted in a filtration chamber, allowing exposure of a standardized area of 0.282 mm2. All specimens were treated with 35% liquid phosphoric acid for 15 seconds to maximize dentin permeability. Then they were randomly allocated to five different conditions: C: placebo gel (negative control); HP: Whiteness HP Maxx (WM)-35% hydrogen peroxide; PA-HP: WM preceded by phosphoric acid etching for 15 seconds. HP-E: WM associated to a light source and PA-HP-E: WM preceded by phosphoric acid etching for 15 seconds and associated to a light source. The bleaching agent was applied for 10 minutes with intermediate agitation at 5 minutes. In the groups associated with a light source, it was activated for 30 seconds every 5 minutes. Afterwards, the permeability (Lp) was measured using the Flodec device. The data were collected and analyzed with one-way ANOVA and Tukey's test (P< 0.05). RESULTS: Mean and standard deviation of Lp were: C = 1.92 (1.04); HP = 1.14 (0.29); PA-HP = 14.40 (8.62); HP-E = 4.18 (5.14); PA-HP-E = 27.32 (13.24). Data revealed that phosphoric acid etching could increase the dentin permeability, while the bleaching agent or light curing alone did not.


Assuntos
Permeabilidade da Dentina/efeitos dos fármacos , Clareadores Dentários/farmacologia , Clareamento Dental/métodos , Condicionamento Ácido do Dente/métodos , Compostos Inorgânicos de Carbono/química , Dentina/efeitos dos fármacos , Líquido Dentinal/efeitos dos fármacos , Humanos , Peróxido de Hidrogênio/farmacologia , Luz , Ácidos Fosfóricos/farmacologia , Placebos , Compostos de Silício/química , Camada de Esfregaço , Fatores de Tempo
16.
Cleft Palate Craniofac J ; 50(4): 394-9, 2013 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-22292803

RESUMO

Objective : To evaluate the prevalence, types, location, and characteristics of enamel defects in anterior permanent teeth of patients with complete unilateral and bilateral cleft lip and palate, as well as the relation with the cleft. Setting : Hospital for Rehabilitation of Craniofacial Anomalies, Bauru, São Paulo, Brazil. Participants : Eighty patients of both genders, 12 years and older, with unilateral or bilateral cleft lip and palate. Methods : A single examiner carried out clinical examination under artificial light with a dental probe and mirror after drying teeth according to the modified DDE index. Results : Seventy-four of 80 patients presented with at least one tooth affected by enamel defects: 165 of 325 evaluated teeth (50.8%) presented enamel defects, with hypoplasia being the most prevalent (50.7%), followed by diffuse opacity (23.1%) and demarcated opacity (18.4%). The most affected tooth was 21 (36.5%), followed by 11 (34%), located at the middle (40%) and incisal (33%) thirds. Most defects occur at the buccal surface (47.7%), followed by the distal (22.7%), the mesial (19%), and the palatal (10.6%) surfaces. A significant relationship was found between the cleft side and enamel defects. Conclusion : Upper anterior teeth of patients with complete cleft lip and palate present a high prevalence of enamel defects; the highest percentage on the cleft side suggests that the cleft does influence the occurrence of enamel defects in permanent teeth.


Assuntos
Fenda Labial , Fissura Palatina , Fenda Labial/epidemiologia , Fissura Palatina/epidemiologia , Esmalte Dentário , Humanos , Prevalência
17.
J. appl. oral sci ; 20(6): 613-619, Nov.-Dec. 2012. ilus, tab
Artigo em Inglês | LILACS | ID: lil-660631

RESUMO

OBJECTIVE: This study evaluated the performance of different adhesive systems in fiber post placement aiming to clarify the influence of different hydrophobic experimental blend adhesives, and of one commercially available adhesive on the frictional retention during a luting procedure. MATERIAL AND METHODS: One luting agent (70 Wt% BisGMA, 28.5% TEGDMA; 1.5% p-tolyldiethanolamine) to cement fiber posts into root canals was applied with 4 different adhesive combinations: Group 1: The etched roots were rinsed with water for 30 s to remove the phosphoric acid, then rinsed with 99.6% ethanol for 30 s, and blotdried. A trial adhesive (base to catalyst on a 1:1 ratio) was used with an experimental luting agent (35% Bis-GMA, 14.37% TeGDMA, 0.5% eDMAB, 0.13% CQ); Group 2: A trial adhesive (base to catalyst on a 1:2 ratio) was luted as in Group 1; Group 3: One-Step Plus (OSP, Bisco Inc.) following the ethanol bonding technique in combination with the luting agent as in Group 1; Group 4: OSP strictly following the manufacturer's instructions using the luting agent as in Group 1. The groups were challenged with push-out tests. Posted root slices were loaded until post segment extrusion in the apical-coronal direction. Failure modes were analyzed under scanning electron microscopy. RESULTS: Push-out strength was not significantly influenced by the luting agent (p>0.05). No statistically significant differences among the tested groups were found as Group 1 (exp 1 - ethanol-wet bonding technique)=Group 2 (exp 2 - ethanol-wet bonding technique)=Group 3 (OSP - ethanol-wet bonding technique)=Group 4 (control, OSP - water-wet bonding technique) (p>0.05). The dominating failure modes in all the groups were cohesive/adhesive failures, which were predominantly observed on the post/luting agent interface. CONCLUSIONS: The results of this study support the hypothesis that the proposal to replace water with ethanol to bond fiber posts to the root canal using highly hydrophobic resin is plausible, but this seems to be more the proof of a concept than a clinically applicable procedure.


Assuntos
Humanos , Colagem Dentária/métodos , Cavidade Pulpar/química , Adesivos Dentinários/química , Microscopia Eletrônica de Varredura , Técnica para Retentor Intrarradicular , Falha de Restauração Dentária , Cavidade Pulpar/efeitos dos fármacos , Dentina/química , Dentina/efeitos dos fármacos , Interações Hidrofóbicas e Hidrofílicas , Teste de Materiais , Distribuição Aleatória , Estatísticas não Paramétricas , Propriedades de Superfície
18.
J Appl Oral Sci ; 20(6): 613-9, 2012.
Artigo em Inglês | MEDLINE | ID: mdl-23329242

RESUMO

OBJECTIVE: This study evaluated the performance of different adhesive systems in fiber post placement aiming to clarify the influence of different hydrophobic experimental blend adhesives, and of one commercially available adhesive on the frictional retention during a luting procedure. MATERIAL AND METHODS: One luting agent (70 Wt% BisGMA, 28.5% TEGDMA; 1.5% p-tolyldiethanolamine) to cement fiber posts into root canals was applied with 4 different adhesive combinations: group 1: The etched roots were rinsed with water for 30 s to remove the phosphoric acid, then rinsed with 99.6% ethanol for 30 s, and blotdried. A trial adhesive (base to catalyst on a 1:1 ratio) was used with an experimental luting agent (35% Bis-GMA, 14.37% TeGDMA, 0.5% eDMAB, 0.13% CQ); group 2: A trial adhesive (base to catalyst on a 1:2 ratio) was luted as in group 1; group 3: One-Step Plus (OSP, Bisco Inc.) following the ethanol bonding technique in combination with the luting agent as in group 1; group 4: OSP strictly following the manufacturer's instructions using the luting agent as in group 1. The groups were challenged with push-out tests. Posted root slices were loaded until post segment extrusion in the apical-coronal direction. Failure modes were analyzed under scanning electron microscopy. RESULTS: Push-out strength was not significantly influenced by the luting agent (p>0.05). No statistically significant differences among the tested groups were found as group 1 (exp 1--ethanol-wet bonding technique)=group 2 (exp 2--ethanol-wet bonding technique)=Group 3 (OSP--ethanol-wet bonding technique)=group 4 (control, OSP--water-wet bonding technique) (p>0.05). The dominating failure modes in all the groups were cohesive/adhesive failures, which were predominantly observed on the post/luting agent interface. CONCLUSIONS: The results of this study support the hypothesis that the proposal to replace water with ethanol to bond fiber posts to the root canal using highly hydrophobic resin is plausible, but this seems to be more the proof of a concept than a clinically applicable procedure.


Assuntos
Colagem Dentária/métodos , Cavidade Pulpar/química , Adesivos Dentinários/química , Microscopia Eletrônica de Varredura , Técnica para Retentor Intrarradicular , Cavidade Pulpar/efeitos dos fármacos , Falha de Restauração Dentária , Dentina/química , Dentina/efeitos dos fármacos , Humanos , Interações Hidrofóbicas e Hidrofílicas , Teste de Materiais , Distribuição Aleatória , Estatísticas não Paramétricas , Propriedades de Superfície
19.
J Dent ; 39(12): 834-40, 2011 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-21945449

RESUMO

OBJECTIVES: The aim of this study was to evaluate the Knoop microhardness (KHN) and chemical composition of high-viscous glass-ionomer cement (HVGIC) after 10 years of clinical service. METHODS: Six HVGIC samples were cut from 10-year ART restorations. The sections were embedded in acrylic moulds with their longitudinal profile exposed. KHN was determined by performing three sequences of five indentations at 10, 30, 50, 70 and 90 µm of HVGIC outer surface. For the control group (n=6), HVGIC specimens were stored in distilled water for 24 months. Hardness measurements were taken at days 7, 30, 60, 120, 180, 360, and 720. For chemical analysis using SEM-EDX, 10-year and control specimens were dehydrated and coated with carbon. Data were analysed using T-test and ANOVA/Tukey's test (p<0.05). RESULTS: A significant KHN increase was observed in the control group up to the 180-day period. From this point the values stabilized and no more significant differences were found between the 10-year and the control KHN values. No statistical differences were observed amongst the KHN from inner distances compared to the outer surface of the 10-year HVGIC specimens. In one 10-year specimen, SEM-images identified the transformation of HVGIC in an altered layer with no glass filler particles detectable, and raised Ca, K and P contents. CONCLUSIONS: KHN values of ten-year HVGIC specimens were similar to the control group values at 180-day storage period. Except for one 10-year specimen in that an altered layer could be seen, chemical composition was similar amongst the depths evaluated.


Assuntos
Tratamento Dentário Restaurador sem Trauma/métodos , Cimentos de Ionômeros de Vidro/química , Alumínio/análise , Cálcio/análise , Análise do Estresse Dentário/instrumentação , Diamante/química , Fluoretos/análise , Seguimentos , Cimentos de Ionômeros de Vidro/análise , Dureza , Humanos , Microscopia Eletrônica de Varredura , Fósforo/análise , Potássio/análise , Dióxido de Silício/análise , Espectrometria por Raios X , Estrôncio/análise , Propriedades de Superfície , Viscosidade , Água/química
20.
Rev. dental press estét ; 8(3): 74-88, jul.-set. 2011. ilus
Artigo em Português | LILACS, BBO - Odontologia | ID: lil-654763

RESUMO

Paciente do sexo feminino, 25 anos de idade, procurou tratamento estético queixando-se do escurecimento do dente 21. Foram realizados exames clínicos e readiográficos, comprovando a alteração de cor e o tratamento endodôntico satisfatório. O plano de tratamento foi proposto para estabelkecer a função e a estética do dente 21 (técnica mediata), associado ao clareamento externo, em consultório, dos dentes #15 a #25 e #35 a #45 co peróxido de hidrogênio a 35 por cento, ativado com luz híbrida, a fim de que fossem uniformizadas as tonalidades de cor dos dentes. Posteriormente, um pino de fibra de vidro foi fixado no canal radicular, com cimento de ionômero de vidro convencional, seguido pela restauração da abertura coronária com resina composta. As etapas do tratamento revelaram que o planejamento correto combinado com o conhecimento das técnicas disponíveis e das propriedades dos materiais são essenciais para a obtenção da excelência estética e funcional dos dentes em questão.


A 25-year-old woman was seeking for aesthetic treatment for her #21 tooth. Clinical and radiographic examinations were done confirming color change and satisfactory endodontic treatment. Thus, a treatment was proposed to reestablish function and aesthetics to the compromised teeth. The #21 tooth received internal bleaching and #15 to #25 and #35 to #45 teeth received external bleaching with a 35% hydrogen peroxide gel activated by hybrid light. This was performed in order to standardize the tonalities of the color of the teeth. Subsequently, a glass-fiber post was fixed inside the radicular conduct with conventional glass-ionomer cement and the endodontic coronal aperture was restored with composite resin. Treatment steps showed that the correct planning associated with the knowledge of available techniques and material properties are essential for reaching functional and aesthetic excellence.


Assuntos
Humanos , Feminino , Adulto , Resinas Compostas , Clareamento Dental/métodos , Pinos Dentários , Endodontia , Estética Dentária , Peróxido de Hidrogênio , Clareamento Dental
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