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1.
Molecules ; 26(19)2021 Sep 23.
Artigo em Inglês | MEDLINE | ID: mdl-34641304

RESUMO

The current research work illustrates an economical and rapid approach towards the biogenic synthesis of silver nanoparticles using aqueous Punica granatum leaves extract (PGL-AgNPs). The optimization of major parameters involved in the biosynthesis process was done using Box-Behnken Design (BBD). The effects of different independent variables (parameters), namely concentration of AgNO3, temperature and ratio of extract to AgNO3, on response viz. particle size and polydispersity index were analyzed. As a result of experiment designing, 17 reactions were generated, which were further validated experimentally. The statistical and mathematical approaches were employed on these reactions in order to interpret the relationship between the factors and responses. The biosynthesized nanoparticles were initially characterized by UV-vis spectrophotometry followed by physicochemical analysis for determination of particle size, polydispersity index and zeta potential via dynamic light scattering (DLS), SEM and EDX studies. Moreover, the determination of the functional group present in the leaves extract and PGL-AgNPs was done by FTIR. Antibacterial and antibiofilm efficacies of PGL-AgNPs against Gram-positive and Gram-negative bacteria were further determined. The physicochemical studies suggested that PGL-AgNPs were round in shape and of ~37.5 nm in size with uniform distribution. Our studies suggested that PGL-AgNPs exhibit potent antibacterial and antibiofilm properties.

2.
Sci Rep ; 11(1): 19242, 2021 Sep 28.
Artigo em Inglês | MEDLINE | ID: mdl-34584124

RESUMO

Highly selective and sensitive 2,7-naphthyridine based colorimetric and fluorescence "Turn Off" chemosensors (L1-L4) for detection of Ni2+ in aqueous media are reported. The receptors (L1-L4) showed a distinct color change from yellow to red by addition of Ni2+ with spectral changes in bands at 535-550 nm. The changes are reversible and pH independent. The detection limits for Ni2+ by (L1-L4) are in the range of 0.2-0.5 µM by UV-Visible data and 0.040-0.47 µM by fluorescence data, which is lower than the permissible value of Ni2+ (1.2 µM) in drinking water defined by EPA. The binding stoichiometries of L1-L4 for Ni2+ were found to be 2:1 through Job's plot and ESI-MS analysis. Moreover the receptors can be used to quantify Ni2+ in real water samples. Formation of test strips by the dip-stick method increases the practical applicability of the Ni2+ test for "in-the-field" measurements. DFT calculations and AIM analyses supported the experimentally determined 2:1 stoichiometries of complexation. TD-DFT calculations were performed which showed slightly decreased FMO energy gaps due to ligand-metal charge transfer (LMCT).

3.
Spectrochim Acta A Mol Biomol Spectrosc ; 265: 120356, 2021 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-34536896

RESUMO

A selective and sensitive detection of L-cysteine (Cys) is an important tool for various biological studies. Here, Au nanoparticles (NPs) were prepared by chemical reduction technique. The probe was developed to detect and quantify Cys in the presence of Cr3+ ions which acts as a cross linker. The citrate capped Au NPs probe was analyzed by UV-visible spectrophotometry, TEM, EDAX, FTIR, DLS, XPS and zetasize. The zeta potential and effective size of Au NPs were -41.22 mV and 12 nm, respectively. The Cys interaction with Au NPs showed drastic colour variation from red to purple and colourless with rapid response time of 1 min. The limit of detection (LOD) of Au NPs probe was as low as 0.012 nM. The TEM image of Au NPs after Cys interaction verified the aggregation that resulted in colour change. The XPS core level scans of Au 4f showed 0.3 eV red shift when Cyswas interacted. The Au NPs sensor is highly selective for Cys with excellent reproducibility. Acidic pH slightly favored Cys detection. Further, the probe was applied to estimate Cys quantity from milk, urine, blood and environmental augmented samples in the presence of other amino acids . The study suggests that the proposed Au NPs could detect Cys with high accuracy from various biological samples.

4.
PLoS One ; 16(8): e0253853, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34432788

RESUMO

Ophioceras is accommodated in the monotypic family Ophioceraceae (Magnaporthales, Sordariomycetes), and the genus is delimited based on molecular data. During an ongoing survey of bambusicolous fungi in southwest China, we collected a submerged decaying branch of bamboo from Sichuan Province, China and an Ophioceras species occurring on this substrate was observed and isolated. An Ophioceras taxon was delimited based on morphological characteristics and combined LSU, RPB1 and ITS sequence analyses and is described as Ophioceras sichuanense sp. nov. The species formed a well-supported clade basal to Ophioceras (100% ML, 1.00 PP). Based on the updated phylogenetic tree of Magnaporthales, Ceratosphaerella castillensis (generic type) and C. rhizomorpha formed a clade within Ophioceras and morphologically resemble Ophioceras. Therefore, Ceratosphaerella is synonymized under Ophioceras. The phylogenetic relationships of Ophioceras are discussed in relation to morphological similarities of genera in Magnaporthales. The generic circumscription of Ophioceras is emended.

5.
Molecules ; 26(15)2021 Jul 29.
Artigo em Inglês | MEDLINE | ID: mdl-34361745

RESUMO

The edible parts of the plants Camellia sinensis, Vitis vinifera and Withania somnifera were extensively used in ancient practices such as Ayurveda, owing to their potent biomedical significance. They are very rich in secondary metabolites such as polyphenols, which are very good antioxidants and exhibit anti-carcinogenic properties. This study aims to evaluate the anti-cancerous properties of these plant crude extracts on human liver cancer HepG2 cells. The leaves of Camellia sinensis, Withania somnifera and the seeds of Vitis vinifera were collected and methanolic extracts were prepared. Then, these extracts were subjected to DPPH, α- amylase assays to determine the antioxidant properties. A MTT assay was performed to investigate the viability of the extracts of HepG2 cells, and the mode of cell death was detected by Ao/EtBr staining and flow cytometry with PI Annexin- V FITC dual staining. Then, the protein expression of BAX and BCl2 was studied using fluorescent dye to determine the regulation of the BAX and BCl2 genes. We observed that all the three extracts showed the presence of bioactive compounds such as polyphenols or phytochemicals. The W. somnifera bioactive compounds were found to have the highest anti-proliferative activity on human liver cancer cells.


Assuntos
Antineoplásicos Fitogênicos/farmacologia , Antioxidantes/farmacologia , Camellia sinensis/química , Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos , Vitis/química , Withania/química , Alcaloides/química , Alcaloides/isolamento & purificação , Antineoplásicos Fitogênicos/química , Antineoplásicos Fitogênicos/isolamento & purificação , Antioxidantes/química , Antioxidantes/isolamento & purificação , Compostos de Bifenilo/antagonistas & inibidores , Compostos de Bifenilo/química , Morte Celular/efeitos dos fármacos , Flavonoides/química , Flavonoides/isolamento & purificação , Células Hep G2 , Humanos , Picratos/antagonistas & inibidores , Picratos/química , Extratos Vegetais/química , Folhas de Planta/química , Proteínas Proto-Oncogênicas c-bcl-2/genética , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , Sementes/química , Transdução de Sinais , Taninos/química , Taninos/isolamento & purificação , Terpenos/química , Terpenos/isolamento & purificação , alfa-Amilases/genética , alfa-Amilases/metabolismo , Proteína X Associada a bcl-2/genética , Proteína X Associada a bcl-2/metabolismo
6.
Spectrochim Acta A Mol Biomol Spectrosc ; 264: 120253, 2021 Aug 06.
Artigo em Inglês | MEDLINE | ID: mdl-34391992

RESUMO

Globally, the environmental pollution is one of the major issues causing toxicity towards human and aquatic life. We have developed a facile and innovative sensing approach for detection of sulphide ions (S2-) present in the aqueous media using Ag0 decorated Cr2S3 NPs embedded on PVP matrix (Ag/Cr2S3-PVP). Based on the SPR phenomena, the detection of S2- ions was established. The nanohybrid was characterized using various techniques such as UV-vis spectrophotometer, High-Resolution Transmission Electron Microscopy (HR-TEM), Thermal Gravimetric Analysis (TGA), X-ray diffraction analysis(XRD), Energy-dispersive X-ray spectroscopy (EDS), Fourier-transform infrared spectroscopy (FT-IR), Scanning Electron Microscopy (SEM), and X-ray photoelectron spectroscopy (XPS). The yellowish colour of Ag/Cr2S3-PVP nanohybrid turned to brown colour in presence of S2- ions. The selectivity and sensitivity of the prepared probe was studied against the other interfering metal ions. In addition, the effect of different concentration of S2- ions in the nanohybrid solution was investigated and the Limit of detection (LOD) was found to be 6.6 nM. The good linearity was found over the range of 10 nM to 100 µM with R2 value of 0.981. The paper strip based probe was developed for rapid onsite monitoring of S2- ions. The proposed method is found to be cost-effective, rapid, and simple. We have validated the practical applicability of the prepared probe for determining the concentration of S2- ions in real water samples.

7.
Sci Rep ; 11(1): 3824, 2021 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-33589661

RESUMO

The present study aims at developing PGMD (poly-glycerol-malic acid-dodecanedioic acid)/curcumin nanoparticles based formulation for anticancer activity against breast cancer cells. The nanoparticles were prepared using both the variants of PGMD polymer (PGMD 7:3 and PGMD 6:4) with curcumin (i.e. CUR NP 7:3 and CUR NP 6:4). The size of CUR NP 7:3 and CUR NP 6:4 were found to be ~ 110 and 218 nm with a polydispersity index of 0.174 and 0.36, respectively. Further, the zeta potential of the particles was - 18.9 and - 17.5 mV for CUR NP 7:3 and CUR NP 6:4, respectively. The entrapment efficiency of both the nanoparticles was in the range of 75-81%. In vitro anticancer activity and the scratch assay were conducted on breast cancer cell lines, MCF-7 and MDA-MB-231. The IC50 of the nanoformulations was observed to be 40.2 and 33.6 µM at 48 h for CUR NP 7:3 and CUR NP 6:4, respectively, in MCF-7 cell line; for MDA-MB-231 it was 43.4 and 30.5 µM. Acridine orange/EtBr and DAPI staining assays showed apoptotic features and nuclear anomalies in the treated cells. This was further confirmed by western blot analysis that showed overexpression of caspase 9 indicating curcumin role in apoptosis.

8.
Bioorg Chem ; 107: 104626, 2021 02.
Artigo em Inglês | MEDLINE | ID: mdl-33450545

RESUMO

Vincamine, a well-known plant alkaloid, has been used as a dietary supplement and as a peripheral vasodilator to combat aging in humans. In this study, for the very first time, we demonstrated that vincamine can function as an anticancer agent in a human alveolar basal epithelial cell line A549 (IC50 = 309.7 µM). The anticancer potential of vincamine in A549 cells was assessed by molecular assays to determine cell viability, generation of intracellular ROS, nuclear condensation, caspase-3 activity and inhibition, and change in mitochondrial membrane potential (ΔΨm). In silico studies predicted that the anti-proliferative potential of vincamine is enhanced by its interaction with the apoptotic protein caspase-3, and that this interaction is driven by two hydrogen bonds and has a high free energy of binding (-5.64 kcal/mol) with an estimated association constant (Ka) of 73.67 µM. We found that vincamine stimulated caspase-3-dependent apoptosis and lowered mitochondrial membrane potential, which ultimately led to cytochrome C release. Vincamine was also found to quench hydroxyl free radicals and deplete iron ions in cancer cells. As a dietary supplement, vincamine is almost non-toxic in BEAS-2B and 3T3-L1 cells. Therefore, we propose that vincamine represents a safe anticancer agent in lung cancer cells. Its role in other cancers has yet to be explored.


Assuntos
Antineoplásicos/química , Células A549 , Alcaloides/química , Alcaloides/farmacologia , Antineoplásicos/farmacologia , Apoptose/efeitos dos fármacos , Sítios de Ligação , Caspase 3/química , Caspase 3/metabolismo , Inibidores de Caspase/farmacologia , Domínio Catalítico , Sobrevivência Celular/efeitos dos fármacos , Humanos , Cinética , Potencial da Membrana Mitocondrial/efeitos dos fármacos , Simulação de Acoplamento Molecular , Espécies Reativas de Oxigênio/metabolismo , Termodinâmica , Vincamina/química , Vincamina/farmacologia
9.
Chemosphere ; 270: 129474, 2021 May.
Artigo em Inglês | MEDLINE | ID: mdl-33445153

RESUMO

The marine bacterium able to consume DDT as the nutrient source was isolated from sea water which was identified as Paracoccus sp. DDT-21 based on 16 S rDNA gene sequence and Gram negative rod, obligate aerobic, non-motile biochemical characteristics. The isolate can degrade over 80% of the DDT, at a concentration of 50 mg/L in MSM in 72 h. Time and pollutant (DDT) dependent growth studies indicated that the isolate Paracoccus sp., DDT-21 significantly degrade the DDT and tolerates under DDT stress up to 50 mg/L. The DDT degradation capability of the strain Paracoccus sp. DDT-21 was found to be 5 ˃ 10 ˃ 15 ˃ 25 ˃ 50 mg/L DDT. The high concentrations (75 and 100 mg/L) of DDT showed significant decrease in DDT degradation. The optimal DDT degradation (∼90.0%) was observed at 6 g/L of yeast extract, 6% of glucose in pH 7.0 at 35 °C with 72 h of incubation as constant. Furthermore, four metabolites were observed by GC-MS analysis such as, DDE, DDD, DDMU, and DDA. The obtained results indicate that the isolate Paracoccus sp. DDT-21 is a promising candidate for the removal and/or detoxification of DDT in the environment.


Assuntos
Poluentes Ambientais , Paracoccus , Bactérias , Biodegradação Ambiental , DDT/análise , Paracoccus/genética , Filogenia , RNA Ribossômico 16S
10.
Environ Pollut ; 269: 116218, 2021 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-33316490

RESUMO

The current study for the first time demonstrates the interference of a free-living, N2-fixing, and nanoparticle (NP) tolerant Azotobacter salinestris strain ASM recovered from metal-polluted soil with tomato plant-metal oxide NPs (ZnO, CuO, Al2O3, and TiO2) interactions in a sandy clay loam soil system with bulk materials as control. Tomato plants were grown till full maturity in soils amended with 20-2000 mg kg-1 of each metal-oxide NP with and without seed biopriming and root-inoculation of A. salinestris. A. salinestris was found metabolically active, producing considerably high amounts of bioactive indole-3-acetic-acid, morphologically unaffected, and with low alteration of cell membrane permeability under 125-1500 µgml-1 of NPs. However, ZnO-NPs slightly alter bacterial membrane permeability. Besides, A. salinestris secreted significantly higher amounts of extracellular polymeric substance (EPS) even under NP exposure, which could entrap the NPs and form metal-EPS complex as revealed and quantified by SEM-EDX. NPs were also found adsorbed on bacterial biomass. EPS stabilized the NPs and provided negative zeta potential to NPs. Following soil application, A. salinestris improved the plant performance and augmented the yield of tomato fruits and lycopene content even in NPs stressed soils. Interestingly, A. salinestris inoculation enhanced photosynthetic pigment formation, flower attributes, plant and fruit biomass, and reduced proline level. Bacterial inoculation also reduced the NP's uptake and accumulation significantly in vegetative organs and fruits. The organ wise order of NP's internalization was roots > shoots > fruits. Conclusively, A. salinestris inoculation could be an alternative to increase the production of tomato in metal-oxide NPs contaminated soils.


Assuntos
Lycopersicon esculentum , Nanopartículas Metálicas , Nanopartículas , Poluentes do Solo , Óxido de Zinco , Azotobacter , Matriz Extracelular de Substâncias Poliméricas/química , Óxidos , Raízes de Plantas/química , Poluentes do Solo/análise
11.
Chemosphere ; 262: 128388, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33182095

RESUMO

Arbitrary use of insecticides in agricultural practices cause severe environmental hazard that adversely affects the growth and productivity of edible crops. Considering theses, the aim of the present study was to evaluate the toxicological potential of two neonicotinoid insecticides, imidacloprid (IMID) and thiamethoxam (THIA) using chickpea as a test crop. Application of insecticides at three varying doses revealed a gradual decrease in biological performance of chickpea plants which however, varied noticeably among insecticides and their doses. Significant (P ≤ 0.05) decline in germination efficiency, length of plant organs under in vitro condition was observed in a dose related manner. Among insecticides, 300 µgIMIDkg-1 (3X dose) maximally and significantly (P ≤ 0.05) inhibited germination efficiency, vigor index, length, dry matter accumulation, photosynthetic pigment formation, nodule volume and mass, nutrient uptake, grain yield and protein over untreated control. In contrast, 75 µgTHIAkg-1 (3X dose) considerably declined the leghaemoglobin content, shoot phosphorus and root nitrogen. Enhanced expression of stress biomarkers including proline, malondialdehyde (MDA), and antioxidant defence enzymes was noticed in the presence of insecticides. For instance, at 3X IMID, shoot proline, MDA, ascorbate peroxidase (APX), guaiacol peroxidase (GPX) and peroxidase (POD) were increased significantly (P ≤ 0.05) by 66%, 81%, 36% and 35%, respectively. Additionally, electrolyte leakage was maximally (77%) increased at 3X dose of IMID, whereas, H2O2 in foliage was maximally accumulated (0.0156 µ moles min-1 g-1 fw) at 3X dose of THIA which was 58% greater than untreated foliage. A clear distortion/damage in tip and surface of roots and ultrastructural deformation in xylem and phloem of plant tissues as indication of insecticidal phytotoxicity was observed under scanning electron microscope (SEM). For oxidative stress and cytotoxicity assessment, root tips were stained with a combination of acridine orange and propidium iodide, and Evan blue dyes and examined. Confocal laser scanning microscopic (CLSM) images of roots revealed a 10-fold and 13.5-fold increase in red and blue fluorescence when 3X IMID treated roots were assessed quantitatively. Conclusively, the present investigation recommends that a careful and protected approach should be adopted before the application of insecticides in agricultural ecosystems.


Assuntos
Cicer/fisiologia , Inseticidas/toxicidade , Neonicotinoides/toxicidade , Antioxidantes/metabolismo , Ascorbato Peroxidases/metabolismo , Cicer/enzimologia , Ecossistema , Germinação , Peróxido de Hidrogênio/metabolismo , Inseticidas/metabolismo , Malondialdeído/metabolismo , Nitrocompostos , Estresse Oxidativo , Peroxidase , Fotossíntese
12.
Biomolecules ; 10(12)2020 12 16.
Artigo em Inglês | MEDLINE | ID: mdl-33339083

RESUMO

This study aims to determine the anticancer efficacy of diosgenin encapsulated poly-glycerol malate co-dodecanedioate (PGMD) nanoparticles. Diosgenin loaded PGMD nanoparticles (variants 7:3 and 6:4) were synthesized by the nanoprecipitation method. The synthesis of PGMD nanoparticles was systematically optimized employing the Box-Behnken design and taking into account the influence of various independent variables such as concentrations of each PGMD, diosgenin and PF-68 on the responses such as size and PDI of the particles. Mathematical modeling was done using the Quadratic second order modeling method and response surface analysis was undertaken to elucidate the factor-response relationship. The obtained size of PGMD 7:3 and PGMD 6:4 nanoparticles were 133.6 nm and 121.4 nm, respectively, as measured through dynamic light scattering (DLS). The entrapment efficiency was in the range of 77-83%. The in vitro drug release studies showed diffusion and dissolution controlled drug release pattern following Korsmeyer-Peppas kinetic model. Furthermore, in vitro morphological and cytotoxic studies were performed to evaluate the toxicity of synthesized drug loaded nanoparticles in model cell lines. The IC50 after 48 h was observed to be 27.14 µM, 15.15 µM and 13.91 µM for free diosgenin, PGMD 7:3 and PGMD 6:4 nanoparticles, respectively, when administered in A549 lung carcinoma cell lines.


Assuntos
Diosgenina/farmacologia , Sistemas de Liberação de Medicamentos/métodos , Malatos/química , Nanopartículas/química , Células A549 , Laranja de Acridina , Antineoplásicos/farmacologia , Apoptose , Sobrevivência Celular/efeitos dos fármacos , Portadores de Fármacos , Liberação Controlada de Fármacos , Difusão Dinâmica da Luz , Etídio/química , Humanos , Técnicas In Vitro , Concentração Inibidora 50 , Cinética , Luz , Modelos Teóricos , Tamanho da Partícula , Polímeros/química , Espalhamento de Radiação
13.
Molecules ; 25(21)2020 Oct 30.
Artigo em Inglês | MEDLINE | ID: mdl-33143044

RESUMO

The increasing interest in developing potent non-toxic drugs in medicine is widening the opportunities for studying the usage of nanostructures in the treatment of various diseases. The present work reports a method for a facile and an eco-friendly synthesis of silver nanoparticles (AgNPs) using Terminalia chebula fruit extract (TCE). The obtained AgNPs was characterized by using different spectroscopic and microscopic techniques. The analysis of the results revealed that the as-obtained AgNPs have spherical morphology with an average diameter of 22 nm. Furthermore, the preliminary bioactivity evaluations revealed that the bio-conjugation of AgNPs, using TCE, significantly enhanced the antibacterial and anti-breast cancer potentials of the latter. The antibacterial activity of the as-prepared AgNPs showed that B. subtilis was more sensitive towards the AgNPs, followed by P. aeruginosa; while, E. coli and S. mutans showed comparatively minimal sensitivity toward the AgNPs. The IC50 values of TCE, AgNPs and TCE + AgNPs treatment of MCF-7 were found to be 17.53, 14.25 and 6.484 µg/mL, respectively. Therefore, it can be ascertained that the bio-conjugation may provide a headway with regard to the therapeutic employment of T. chebula, upon mechanistically understanding the basis of observed antibacterial and anticancer activities.


Assuntos
Anti-Infecciosos , Bacillus subtilis/crescimento & desenvolvimento , Neoplasias da Mama/tratamento farmacológico , Citotoxinas , Frutas/química , Nanopartículas Metálicas , Extratos Vegetais/química , Prata , Terminalia/química , Anti-Infecciosos/síntese química , Anti-Infecciosos/química , Anti-Infecciosos/farmacologia , Neoplasias da Mama/metabolismo , Neoplasias da Mama/patologia , Linhagem Celular Tumoral , Citotoxinas/síntese química , Citotoxinas/química , Citotoxinas/farmacologia , Feminino , Humanos , Nanopartículas Metálicas/química , Nanopartículas Metálicas/uso terapêutico , Prata/química , Prata/farmacologia
14.
Artigo em Inglês | MEDLINE | ID: mdl-33020389

RESUMO

Excessive use of fungicides in agriculture may result in substantial accumulation of active residues in soil, which affect crop health and yield. We investigated the response of Raphanus sativus (white radish) to fungicides in soil and potential beneficial interactions of radish plants with fungicide-tolerant plant growth-promoting rhizobacteria (PGPR). The PGPR were isolated from cabbage and mustard rhizospheres. Morphological and biochemical characteristics measured using standard methods, together with analysis of partial 16S rRNA gene sequences, revealed that fungicide-tolerant PGPR, isolates PS3 and AZ2, were closely related to Pseudomonas spp. These PGPR survived in the presence of high fungicide concentrations i.e., up to 2400 µg mL-1 carbendazim (CBZM) and 3200 µg mL-1 hexaconazole (HEXA). Bacterial isolates produced plant growth stimulants even under fungicide stress, though fungicides induced surface morphological distortion and alteration in membrane permeability of these bacteria, which was proved by a set of microscopic observations. Fungicides considerably affected the germination efficiency, growth, and physiological development of R. sativus, but these effects were relieved when inoculated with PGPR isolates. For instance, CBZM at 1500 mg kg-1 decreased whole dry biomass by 71%, whole plant length by 54%, total chlorophyll by 50%, protein content by 61%, and carotenoid production by 29%. After applying isolate AZ2 for white radish grown in CBZM (10 mg kg-1)-amended soil, it could improve plant growth and development with increased whole plant dry weight (10%), entire plant length (13%) and total chlorophyll content (18%). Similarly, isolate PS3 enhanced plant survival by relieving plant stress with declined biomarkers, i.e., proline (12%), malondialdehyde (3%), ascorbate peroxidase (6.5%), catalase (18%), and glutathione reductase (4%). Application of isolates AZ2 and PS3 could be effective for remediation of fungicide-contaminated soil and for improving the cultivation of radish plants while minimizing inputs of fungicides.


Assuntos
Fungicidas Industriais/efeitos adversos , Raphanus/crescimento & desenvolvimento , Microbiologia do Solo , Raízes de Plantas , RNA Ribossômico 16S , Raphanus/efeitos dos fármacos , Solo
15.
Molecules ; 25(18)2020 Sep 16.
Artigo em Inglês | MEDLINE | ID: mdl-32947799

RESUMO

Combination therapy using chemically distinct drugs has appeared as one of the promising strategies to improve anticancer treatment efficiency. In the present investigation, poly-(lactic-co-glycolic) acid (PLGA) nanoparticles electrostatically conjugated with polyethylenimine (PEI)-based co-delivery system for epirubicin and paclitaxel (PLGA-PEI-EPI-PTX NPs) has been developed. The PLGA-PEI-EPI-PTX NPs exhibited a monodispersed size distribution with an average size of 240.93 ± 12.70 nm as measured through DLS and 70.8-145 nm using AFM. The zeta potential of 41.95 ± 0.65 mV from -17.45 ± 2.15 mV further confirmed the colloidal stability and PEI modification on PLGA nanoparticles. Encapsulation and loading efficiency along with in vitro release of drug for nanoparticles were done spectrophotometrically. The FTIR analysis of PLGA-PEI-EPI-PTX NPs revealed the involvement of amide moiety between polymer PLGA and PEI. The effect of nanoparticles on the cell migration was also corroborated through wound healing assay. The MTT assay demonstrated that PLGA-PEI-EPI-PTX NPs exhibited considerable anticancer potential as compared to the naïve drugs. Further, p53 protein expression analysed through western blot showed enhanced expression. This study suggests that combination therapy using PLGA-PEI-EPI-PTX NPs represent a potential approach and could offer clinical benefits in the future for lung cancer patients.


Assuntos
Antineoplásicos/química , Portadores de Fármacos/química , Epirubicina/química , Nanopartículas/química , Paclitaxel/química , Copolímero de Ácido Poliláctico e Ácido Poliglicólico/química , Antineoplásicos/metabolismo , Antineoplásicos/farmacologia , Linhagem Celular Tumoral , Movimento Celular/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Liberação Controlada de Fármacos , Epirubicina/metabolismo , Epirubicina/farmacologia , Humanos , Concentração de Íons de Hidrogênio , Cinética , Neoplasias Pulmonares/metabolismo , Neoplasias Pulmonares/patologia , Paclitaxel/metabolismo , Paclitaxel/farmacologia
16.
Pharmaceutics ; 12(8)2020 Jul 29.
Artigo em Inglês | MEDLINE | ID: mdl-32751176

RESUMO

The persistence of multidrug resistance among microorganisms has directed a mandate towards a hunt for the development of alternative therapeutic modalities. In this context, antimicrobial photodynamic therapy (aPDT) is sprouted as a novel strategy to mitigate biofilms and planktonic cells of pathogens. Nanoparticles (NPs) are reported with unique intrinsic and antimicrobial properties. Therefore, silver NPs (AgNPs) were investigated in this study to determine their ability to potentiate the aPDT of photosensitizer against Staphylococcus aureus and Pseudomonas aeruginosa. Biologically synthesized AgNPs were surface coated with methylene blue (MB) and studied for their aPDT against planktonic cells and biofilms of bacteria. The nano-conjugates (MB-AgNPs) were characterized for their size, shape and coated materials. MB-AgNPs showed significant phototoxicity against both forms of test bacteria and no toxicity was observed in the dark. Moreover, activity of MB-AgNPs was comparatively higher than that of the free MB, which concludes that MB-AgNPs could be an excellent alternative to combat antibiotic resistant bacteria.

17.
Int J Biol Macromol ; 164: 911-919, 2020 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-32682970

RESUMO

Mercury is a toxic heavy metal that reaches to the water bodies mainly by coal burning, mining and petrol refining. The study was focused to investigate the application of Ag-cellulose nanocomposite to detect and quantify mercury colorimetrically. The Ag-cellulose nanocomposite was characterized by X-ray diffraction, Transmission electron microscopy, Fourier transform infrared spectroscopy, UV-visible spectroscopy, particle size analyzer and zetasizer. The study identified that the presence of other metal ions did not interfere with the detection of Hg2+ ion by the probe. The prepared Ag-cellulose nanocomposite-phenylalanine conjugate incorporated paper strip showed an excellent result in Hg2+ detection. The Ag-cellulose nanocomposite was used to quantify the unknown concentration of mercury on real sample (environmental sample) and it was found to be highly accurate by confirming with atomic absorption spectrophotometric analysis. The Ag-cellulose nanocomposite showed effective detection at 45 °C, pH 9 and 0.1% of salinity. The Ag-cellulose nanocomposite showed efficient photocatalytic performance under visible light irradiation. The half-life period of MB by Ag-cellulose nanocomposite under visible light was determined to be 90 min. The study suggests the application of prepared probe in photocatalysis and the detection of Hg2+ from various environmental samples.


Assuntos
Celulose/química , Mercúrio/análise , Nanocompostos/química , Prata/química , Catálise , Colorimetria , Íons , Luz , Membranas Artificiais , Metais , Fotoquímica , Fotólise , Sais , Espectroscopia de Infravermelho com Transformada de Fourier , Ressonância de Plasmônio de Superfície , Temperatura
18.
Molecules ; 25(6)2020 Mar 19.
Artigo em Inglês | MEDLINE | ID: mdl-32204366

RESUMO

Nitroaromatic and nitroamine compounds such as 2,4,6-trinitrotoluene (TNT) are teratogenic, cytotoxic, and may cause cellular mutations in humans, animals, plants, and microorganisms. Microbial-based bioremediation technologies have been shown to offer several advantages against the cellular toxicity of nitro-organic compounds. Thus, the current study was designed to evaluate the ability of Trichoderma viride to degrade nitrogenous explosives, such as TNT, by microbiological assay and Gas chromatography-mass spectrometry (GC-MS) analysis. In this study, T. viride fungus was shown to have the ability to decompose, and TNT explosives were used at doses of 50 and 100 ppm on the respective growth media as a nitrogenous source needed for normal growth. The GC/MS analysis confirmed the biodegradable efficiency of TNT, whereas the initial retention peak of the TNT compounds disappeared, and another two peaks appeared at the retention times of 9.31 and 13.14 min. Mass spectrum analysis identified 5-(hydroxymethyl)-2-furancarboxaldehyde with the molecular formula C6H6O3 and a molecular weight of 126 g·mol-1 as the major compound, and 4-propyl benzaldehyde with a formula of C10H12O and a molecular weight of 148 g mol-1 as the minor compound, both resulting from the biodegradation of TNT by T. viride. In conclusion, T. viride could be used in microbial-based bioremediation technologies as a biological agent to eradicate the toxicity of the TNT explosive. In addition, future molecular-based studies should be conducted to clearly identify the enzymes and the corresponding genes that give T. viride the ability to degrade and remediate TNT explosives. This could help in the eradication of soils contaminated with explosives or other toxic biohazards.


Assuntos
Substâncias Explosivas/química , Trichoderma/crescimento & desenvolvimento , Trinitrotolueno/química , Biodegradação Ambiental , Meios de Cultura/análise , Meios de Cultura/química , Cromatografia Gasosa-Espectrometria de Massas , Nitrogênio/química , Poluentes do Solo/química , Trichoderma/metabolismo
19.
Pathogens ; 9(2)2020 Feb 12.
Artigo em Inglês | MEDLINE | ID: mdl-32059467

RESUMO

The application of nanotechnology in medicine is gaining popularity due to its ability to increase the bioavailability and biosorption of numerous drugs. Chrysin, a flavone constituent of Orocylumineicum vent is well-reported for its biological properties. However, its therapeutic potential has not been fully exploited due to its poor solubility and bioavailability. In the present study, chrysin was encapsulated into chitosan nanoparticles using TPP as a linker. The nanoparticles were characterized and investigated for their anti-biofilm activity against Staphylococcus aureus. At sub-Minimum Inhibitory Concentration, the nanoparticles exhibited enhanced anti-biofilm efficacy against S. aureus as compared to its bulk counterparts, chrysin and chitosan. The decrease in the cell surface hydrophobicity and exopolysaccharide production indicated the inhibitory effect of the nanoparticles on the initial stages of biofilm development. The growth curve analysis revealed that at a sub-MIC, the nanoparticles did not exert a bactericidal effect against S. aureus. The findings indicated the anti-biofilm activity of the chrysin-loaded chitosan nanoparticles and their potential application in combating infections associated with S. aureus.

20.
J Photochem Photobiol B ; 203: 111748, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31918235

RESUMO

Nanotechnology is an emerged field to develop the plant mediated metal based nanodrugs by green method. In this current study, the zinc oxide metal based nanoparticles were developed using (Clausena lansium (Lour.) Skeels) Peel aqueous extracts and zinc nitrate. The C.L extract zinc nanoparticleswere indicated by the sharp peak seen at 350 nm utilizing the Ultraviolet-Visible spectroscopy (UV-Vis). The high peaks indicate the presence of phytochemicals and its functional groups in ZnONPs were studied by the Fourier Transform Infrared Spectroscopy (FT-IR). The X-Ray Diffraction analysis (XRD) explores the pattern and structure of ZnONPs as spherical and base-centered monoclinic crystalline shapes. The C.L extract with Zn nanoparticles were spherical in nature and the size of the synthesized particles were about 28.42 nm respectively. The autophagy (Beclin-1, LC3-I, LC3-II and ATG4B) and apoptotic (Bax, Bcl-2 and Caspase-3) proteins were regulated by the treatment with ZnONPs in SH-SY5Y neuroblastoma cells. The DNA loss or damage was occurred in the ZnONPs treatment and it was performed using Comet assay. The ZnONPs treatment generates the ROS in the cells and decreased its stability and viability. Addition of NAC prevents ROS in the cultured SH-SY5Y cells and prevents the cells from the apoptosis. We concluded that the ZnONPs potentially kills the neuroblastoma cells by producing the intracellular ROS.


Assuntos
Antineoplásicos/farmacologia , Autofagia/efeitos dos fármacos , Nanopartículas Metálicas/química , Óxido de Zinco/química , Antineoplásicos/química , Apoptose/efeitos dos fármacos , Proteína Beclina-1/metabolismo , Caspase 3/metabolismo , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Clausena/química , Clausena/metabolismo , Dano ao DNA/efeitos dos fármacos , Química Verde , Humanos , Nanopartículas Metálicas/toxicidade , Neuroblastoma/metabolismo , Neuroblastoma/patologia , Extratos Vegetais/química , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , Espécies Reativas de Oxigênio/metabolismo
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