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1.
Mater Horiz ; 8(5): 1433-1438, 2021 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-34846450

RESUMO

Photosynthesis is regarded as the foundation for sustaining life on our planet. Light-harvesting is the initial step that activates the subsequent photochemical reactions. In the photosystems, chloroplast is the basic light-driven metabolic factory of higher plant cells. However, there is an incomplete match between the solar radiation spectrum and absorption profile of chloroplasts. It is hard for the photosynthetic pigments to fully utilize the sunlight energy. Here, we designed two new aggregation-induced emission (AIE) molecules with activated alkyl groups (TPE-PPO and TPA-TPO). Via a facile metal-free "Click" reaction, we realized the substantial manipulation of live chloroplasts with the AIE luminogens (AIEgens). Owing to the matched photophysical properties, the AIEgens could harvest harmful ultraviolet radiation (HUVR) and photosynthetically inefficient radiation (PIR), and further convert them into photosynthetically active radiation (PAR) for chloroplast absorption. As a result, the conjugated AIEgen-chloroplast exhibited better capability of water splitting and electron separation. It promoted the generation of adenosine triphosphate (ATP), which is an important product of photosynthesis. This work provides an effective strategy for improving plant photosynthesis.

2.
Mater Horiz ; 8(6): 1816-1824, 2021 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-34846510

RESUMO

The lack of rapid and reliable microbial detection and sensing platforms and insufficient understanding of microbial behavior may delay precautions that could be made, which is a great threat to human life and increases the heavy financial burden on society. In this contribution, a dual-aggregation-induced emission luminogen (AIEgen) system is successfully developed for microbial imaging and metabolic status sensing. This system consists of two AIEgens (DCQA and TPE-2BA) that bear positively charged groups or boronic acid groups, providing universal microbial staining ability and specific affinity for dead microbes, respectively. Based on the distinctive fluorescence response produced by the diverse interaction of AIEgens with live or dead microbes, this dual-AIEgen system can detect all the microbes and identify their viabilities. Furthermore, the morphology and metabolic status of a sessile biofilm can also be imaged and monitored. The system exhibits rapid labelling properties that suitable for various microbes, and good biocompatibilities.

3.
Gut Pathog ; 13(1): 61, 2021 Oct 13.
Artigo em Inglês | MEDLINE | ID: mdl-34645489

RESUMO

BACKGROUND: Gas-producing perianal abscess raises the possibility of clostridial infection, with Clostridium perfringens being the most common causative agent, which is highly lethal if untreated timely. As the treatment of clostridial infections often differs from that of non-clostridial infections, which they may closely resemble, the importance of accurate pathogenic organism identification cannot be overemphasized. The 16S rDNA of bacteria is highly conserved within a species and among species of the same genus but demonstrates substantial variation between different species, thus making it a suitable genomic candidate for bacterial detection and identification. CASE PRESENTATION: Here, we report the case of a 53-year-old patient who was admitted to the hospital for a gas-producing perianal abscess. The patient was managed with ceftizoxime and ornidazole and then received debridement and drainage at the lesion on the second day after admission. The bacterial cultures of the patient isolates from the debridement showed a coinfection of Escherichia coli and Enterococcus faecium. Although perianal redness and swelling subsided obviously after the surgery, the patient was febrile to 38.3℃ with his left upper thigh red and swollen, aggravated with tenderness and crepitus. Considering insufficient debridement and the risk of incorrect identification of pathogens, a second debridement and drainage were performed 4 days after the primary operation, and 16S rDNA sequencing of the isolates implicated Clostridium perfringens infection. Given the discrepancies in diagnostic results and the treatment outcomes, Enterococcus faecium was identified as sample contamination, and a diagnosis of coinfection of Clostridium perfringens and Escherichia coli in gas-producing perianal abscess was confirmed. The patient was then successfully treated with meropenem and vancomycin and was discharged at 27 days of admission. CONCLUSIONS: This case represents the first report of coinfection of both clostridial and non-clostridial organisms in gas-producing perianal abscess and the first case reporting the use of 16S rDNA sequencing in the diagnosis of perianal abscess. Timely pathogen identification is critical for treating gas-producing perianal abscess and an antibiotic regimen covering both aerobic and anaerobic organisms is recommended before true pathogens are identified.

4.
JACS Au ; 1(3): 344-353, 2021 Mar 22.
Artigo em Inglês | MEDLINE | ID: mdl-34467298

RESUMO

Photoresponsive polymers have attracted extensive attention due to their tunable functionalities and advanced applications; thus, it is significant to develop facile in situ synthesis strategies, extend polymers family, and establish various applications for photoresponsive polymers. Herein, we develop a catalyst-free spontaneous polymerization of dihaloalkynes and disulfonic acids without photosensitive monomers for the in situ synthesis of photoresponsive polysulfonates at room temperature in air with 100% atom economy in high yields. The resulting polysulfonates could undergo visible photodegradation with strong photoacid generation, leading to various applications including dual-emissive or 3D photopatterning, and practical broad-spectrum antibacterial activity. The halogen-rich polysulfonates also exhibit a high and photoswitched refractive index and could undergo efficient postfunctionalizations to further expand the variety and functionality of photoresponsive heteroatom-containing polyesters.

5.
Nanoscale ; 13(32): 13610-13616, 2021 Aug 28.
Artigo em Inglês | MEDLINE | ID: mdl-34477635

RESUMO

Biofilm-related infections, such as dental plaque, chronic sinusitis, native valve endocarditis, and chronic airway infections in cystic fibrosis have brought serious suffering to patients and financial burden to society. Materials that can eliminate mature biofilms without developing drug resistance are promising tools to treat biofilm-related infections, and thus they are in urgent demand. Herein, we designed and readily prepared organic nanoparticles (NPs) with highly efficient photothermal conversion by harvesting energy via excited-state intramolecular motions and enlarging molar absorptivity. The photothermal NPs can sufficiently eliminate mature bacterial biofilms upon low-power near-infrared laser irradiation. NPs hold great promise for the rapid eradication of bacterial biofilms by photothermal therapy.


Assuntos
Antibacterianos , Nanopartículas , Antibacterianos/farmacologia , Biofilmes , Humanos , Terapia Fototérmica
6.
Adv Healthc Mater ; : e2101055, 2021 Aug 21.
Artigo em Inglês | MEDLINE | ID: mdl-34418306

RESUMO

The emergence of the concept of aggregation-induced emission (AIE) has opened new opportunities in many research areas, such as biopsy analysis, biological processes monitoring, and elucidation of key physiological and pathological behaviors. As a new class of luminescent materials, AIE luminogens (AIEgens) possess many prominent advantages such as tunable molecular structures, high molar absorptivity, high brightness, large Stokes shift, excellent photostability, and good biocompatibility. The past two decades have witnessed a dramatic growth of research interest in AIE, and many AIE-based bioprobes with excellent performance have been widely explored in biomedical fields. This review summarizes some of the latest advancements of AIE molecular probes and AIE nanoparticles (NPs) with regards to biomedical and healthcare applications. According to the research areas, the review is divided into five sections, which are imaging and identification of cells and bacteria, photodynamic therapy, multimodal theranostics, deep tissue imaging, and fluorescence-guided surgery. The challenges and future opportunities of AIE materials in the advanced biomedical fields are briefly discussed. In perspective, the AIE-based bioprobes play vital roles in the exploration of advanced bioapplications for the ultimate goal of addressing more healthcare issues by integrating various cutting-edge modalities and techniques.

7.
Sci Rep ; 11(1): 16140, 2021 08 09.
Artigo em Inglês | MEDLINE | ID: mdl-34373575

RESUMO

Heavy metal Cadmium (Cd2+) pollution has become a severe environmental problem for aquatic organisms. In crustaceans, gills (Gi) and hepatopancreas (Hp) play a vital role in the toxicology. However, in Macrobrachium rosenbergill, there are few researches about gill and hepatopancreases responding to Cd2+ stress at a molecular level. In this study, transcriptomic analysis was applied to characterize gene expression profiles of gills and hepatopancreas of M. rosenbergill after Cd2+ exposure for 0 h, 3 h and 3 d. Six cDNA libraries (Gi 0 h, Gi 3 h, Gi 3 d, Hp 0 h, Hp 3 h, and Hp 3 d) were constructed and a total of 66,676 transcripts and 48,991 unigenes were annotated. Furthermore, differentially expressed genes (DEGs) were isolated by comparing the Cd2+ treated time-point libraries (3 h and 3 d group) with the control library (0 h group). The results showed that most of the DEGs were down-regulated after Cd2+ exposure and the number of DEGs among gill groups were significantly higher than those among hepatopancreas groups. GO functional and KEGG pathway analysis suggested many key DEGs in response to the Cd2+ stress, such as metallothionein and Hemocyanin. Additionally, a total of six DEGs were randomly selected to further identify their expressional profile by qPCR. The results indicated that these DEGs were involved in the response to Cd2+. This comparative transcriptome provides valuable molecular information on the mechanisms of responding to Cd2+ stress in M. rosenbergii, which lays the foundation for further understanding of heavy metal stress.


Assuntos
Cádmio/toxicidade , Palaemonidae/efeitos dos fármacos , Palaemonidae/genética , Animais , Regulação para Baixo/efeitos dos fármacos , Feminino , Perfilação da Expressão Gênica , Regulação da Expressão Gênica/efeitos dos fármacos , Biblioteca Gênica , Brânquias/efeitos dos fármacos , Brânquias/metabolismo , Hepatopâncreas/efeitos dos fármacos , Hepatopâncreas/metabolismo , Masculino , Anotação de Sequência Molecular , Estresse Oxidativo/efeitos dos fármacos , Estresse Oxidativo/genética , Palaemonidae/metabolismo , Poluentes Químicos da Água/toxicidade
8.
ACS Nano ; 2021 Jun 28.
Artigo em Inglês | MEDLINE | ID: mdl-34181408

RESUMO

The development of effective antifungal agents remains a big challenge in view of the close evolutionary relationship between mammalian cells and fungi. Moreover, rapid mutations of fungal receptors at the molecular level result in the emergence of drug resistance. Here, with low tendency to develop drug-resistance, the subcellular organelle mitochondrion is exploited as an alternative target for efficient fungal killing by photodynamic therapy (PDT) of mitochondrial-targeting luminogens with aggregation-induced emission characteristics (AIEgens). With cationic isoquinolinium (IQ) moiety and proper hydrophobicity, three AIEgens, namely, IQ-TPE-2O, IQ-Cm, and IQ-TPA, can preferentially accumulate at the mitochondria of fungi over the mammalian cells. Upon white light irradiation, these AIEgens efficiently generate reactive 1O2, which causes irreversible damage to fungal mitochondria and further triggers the fungal death. Among them, IQ-TPA shows the highest PDT efficiency against fungi and negligible toxicity to mammalian cells, achieving the selective and highly efficient killing of fungi. Furthermore, we tested the clinical utility of this PDT strategy by treating fungal keratitis on a fungus-infected rabbit model. It was demonstrated that IQ-TPA presents obviously better therapeutic effects as compared with the clinically used rose bengal, suggesting the success of this PDT strategy and its great potential for clinical treatment of fungal infections.

9.
Front Bioeng Biotechnol ; 9: 654087, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34150725

RESUMO

The growth plate (GP) is a cartilaginous region situated between the epiphysis and metaphysis at the end of the immature long bone, which is susceptible to mechanical damage because of its vulnerable structure. Due to the limited regeneration ability of the GP, current clinical treatment strategies (e.g., bone bridge resection and fat engraftment) always result in bone bridge formation, which will cause length discrepancy and angular deformity, thus making satisfactory outcomes difficult to achieve. The introduction of cartilage repair theory and cartilage tissue engineering technology may encourage novel therapeutic approaches for GP repair using tissue engineered GPs, including biocompatible scaffolds incorporated with appropriate seed cells and growth factors. In this review, we summarize the physiological structure of GPs, the pathological process, and repair phases of GP injuries, placing greater emphasis on advanced tissue engineering strategies for GP repair. Furthermore, we also propose that three-dimensional printing technology will play a significant role in this field in the future given its advantage of bionic replication of complex structures. We predict that tissue engineering strategies will offer a significant alternative to the management of GP injuries.

10.
Adv Sci (Weinh) ; 8(9): 2001750, 2021 05.
Artigo em Inglês | MEDLINE | ID: mdl-33977040

RESUMO

Bacteria infected cells acting as "Trojan horses" not only protect bacteria from antibiotic therapies and immune clearance, but also increase the dissemination of pathogens from the initial sites of infection. Antibiotics are hard and insufficient to treat such hidden internalized bacteria, especially multidrug-resistant (MDR) bacteria. Herein, aggregation-induced emission luminogens (AIEgens) such as N,N-diphenyl-4-(7-(pyridin-4-yl) benzo [c] [1,2,5] thiadiazol-4-yl) aniline functionalized with 1-bromoethane (TBP-1) and (3-bromopropyl) trimethylammonium bromide (TBP-2) (TBPs) show potent broad-spectrum bactericidal activity against both extracellular and internalized Gram-positive pathogens. TBPs trigger reactive oxygen species (ROS)-mediated membrane damage to kill bacteria, regardless of light irradiation. TBPs effectively kill bacteria without the development of resistance. Additionally, such AIEgens activate mitochondria dependent autophagy to eliminate internalized bacteria in host cells. Compared to the routinely used vancomycin in clinic, TBPs demonstrate comparable efficacy against methicillin-resistant Staphylococcus aureus (MRSA) in vivo. The studies suggest that AIEgens are promising new agents for the treatment of MDR bacteria associated infections.


Assuntos
Antibacterianos/uso terapêutico , Farmacorresistência Bacteriana Múltipla/efeitos dos fármacos , Staphylococcus aureus Resistente à Meticilina/efeitos dos fármacos , Compostos de Amônio Quaternário/farmacologia , Espécies Reativas de Nitrogênio/farmacologia , Infecções Estafilocócicas/tratamento farmacológico , Animais , Modelos Animais de Doenças , Camundongos
11.
Angew Chem Int Ed Engl ; 60(21): 11758-11762, 2021 05 17.
Artigo em Inglês | MEDLINE | ID: mdl-33724623

RESUMO

Extensive recent efforts have been put on the design of high-performance organic near-infrared (NIR) photothermal agents (PTAs), especially over NIR-II bio-window (1000-1350 nm). So far, the development is mainly limited by the rarity of molecules with good NIR-II response. Here, we report organic nanoparticles of intermolecular charge-transfer complexes (CTCs) with easily programmable optical absorption. By employing different common donor and acceptor molecules to form CTC nanoparticles (CT NPs), absorption peaks of CT NPs can be controllably tuned from the NIR-I to NIR-II region. Notably, CT NPs formed with perylene and TCNQ have a considerably red-shifted absorption peak at 1040 nm and achieves a good photothermal conversion efficiency of 42 % under 1064 nm excitation. These nanoparticles were used for antibacterial application with effective activity towards both Gram-negative and Gram-positive bacteria. This work opens a new avenue into the development of efficient PTAs.


Assuntos
Antibacterianos/farmacologia , Nanopartículas/química , Antibacterianos/química , Antibacterianos/efeitos da radiação , Derivados de Benzeno/química , Derivados de Benzeno/farmacologia , Derivados de Benzeno/efeitos da radiação , Escherichia coli/efeitos dos fármacos , Raios Infravermelhos , Testes de Sensibilidade Microbiana , Nanopartículas/efeitos da radiação , Nitrilas/química , Nitrilas/farmacologia , Nitrilas/efeitos da radiação , Perileno/química , Perileno/farmacologia , Perileno/efeitos da radiação , Compostos Policíclicos/química , Compostos Policíclicos/farmacologia , Compostos Policíclicos/efeitos da radiação , Solubilidade , Staphylococcus aureus/efeitos dos fármacos , Eletricidade Estática/efeitos adversos , Compostos de Sulfidrila/química , Compostos de Sulfidrila/farmacologia , Compostos de Sulfidrila/efeitos da radiação , Água/química
12.
Angew Chem Int Ed Engl ; 60(22): 12424-12430, 2021 05 25.
Artigo em Inglês | MEDLINE | ID: mdl-33760356

RESUMO

Silkworm silk is a promising natural biopolymer for textile and biomedical applications for its remarkable flexibility, excellent biocompatibility and controllable biodegradability. The functionalization of silks makes them more versatile for flexible displays and visible bioscaffolds. However, fluorescent silks are normally fabricated through unstable physical absorption or complicated chemical reactions under harsh conditions. Herein, we developed a simple strategy for preparing fluorescent silks. Five aggregation-induced emission luminogens (AIEgens) with activated alkynes were synthesized by rational molecular design, and then reacted with silk fibers through facile metal-free click bioconjugation. The resulting conjugates show bright full-color emissions and high stability. A white light-emitting silk was fabricated by simultaneous bioconjugation with red-, green- and blue-emissive AIEgens. The red-emissive AIEgen-functionalized silks were successfully applied for long-term cell tracking and two-photon bioimaging, demonstrating great potential for tissue engineering and bioscaffold monitoring.


Assuntos
Materiais Biocompatíveis/química , Substâncias Luminescentes/química , Imagem Óptica/métodos , Seda/química , Células A549 , Alcinos/química , Animais , Bombyx/metabolismo , Química Click , Humanos , Microscopia de Fluorescência por Excitação Multifotônica , Pontos Quânticos/química , Engenharia Tecidual
13.
Sci Rep ; 11(1): 2533, 2021 01 28.
Artigo em Inglês | MEDLINE | ID: mdl-33510284

RESUMO

Appropriate drainage duration is vital for the postoperative rehabilitation of patients with breast cancer (BC) undergoing modified radical mastectomy (MRM). To provide better and individualized postoperative management for these patients, this study explored independent predictors of postoperative drainage duration in patients with BC. This was a single-center retrospective cohort study. Patients diagnosed with BC and treated with MRM from May 2016 to April 2020 were randomly divided into training (n = 729) and validation (n = 243) cohorts. Univariate and multivariate Cox analyses revealed that the body mass index, serum albumin level, hypertension, number of total dissected axillary lymph nodes, and ratio of positive axillary lymph nodes were independent predictors of postoperative drainage duration in the training cohort. Based on independent predictors, a nomogram was constructed to predict the median postoperative drainage duration and the probability of retaining the suction drain during this period. This nomogram had good concordance and discrimination both in the training and validation cohorts and could effectively predict the probability of retaining the suction drain during drainage, thus assisting clinicians in predicting postoperative drainage duration and providing individualized postoperative management for patients with BC.


Assuntos
Neoplasias da Mama/cirurgia , Drenagem , Mastectomia Radical Modificada , Adulto , Idoso , Idoso de 80 Anos ou mais , Neoplasias da Mama/diagnóstico , Tomada de Decisão Clínica , Gerenciamento Clínico , Drenagem/métodos , Feminino , Humanos , Mastectomia Radical Modificada/métodos , Pessoa de Meia-Idade , Nomogramas , Curva ROC , Resultado do Tratamento
14.
Biomaterials ; 268: 120598, 2021 01.
Artigo em Inglês | MEDLINE | ID: mdl-33321291

RESUMO

Pathogenic microbes can cause infections or diseases in hosts and they pose ongoing threats to human health. Antibiotics have been taken an active role in treating a wide variety of infections or diseases since they were first introduced in the 1940s. However, the emergence of antibiotic-resistant microbes makes these previously effective drugs invalid regrettably. So it is urgently needed to accelerate research and development for new antimicrobial systems and strategies. Recently, luminogens with aggregation-induced emission characteristics (AIEgens) have emerged as powerful fluorescent tools for microbial detection and antimicrobial therapy. In this review, we highlighted the latest advancements of AIEgen-based biofunctional materials and systems in this research field. AIE fluorescent probes have the advantages of excellent sensitivity and rapid response, which make them useful for ultrafast bacterial imaging, bacteria classification, and pathogen discrimination. Early microbial detection and identification could help us study the mechanism of antibiotic resistance more scientifically. Moreover, the AIEgens-based photosensitizers (AIE-PSs) with strong photosensitization show good performance on the efficient elimination of multidrug-resistant bacteria and intracellular bacteria. At the end of the review, a short perspective on aggregate science is concluded.


Assuntos
Anti-Infecciosos , Fármacos Fotossensibilizantes , Antibacterianos/farmacologia , Antibacterianos/uso terapêutico , Anti-Infecciosos/farmacologia , Farmacorresistência Bacteriana Múltipla , Corantes Fluorescentes , Humanos , Fármacos Fotossensibilizantes/farmacologia
15.
J Tissue Eng ; 11: 2041731420947242, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32913623

RESUMO

A diabetic foot ulcer (DFUs) is a state of prolonged chronic inflammation, which can result in amputation. Different from normal skin wounds, various commercially available dressings have not sufficiently improved the healing of DFUs. In this study, a novel self-healing hydrogel was prepared by in situ crosslinking of N-carboxyethyl chitosan (N-chitosan) and adipic acid dihydrazide (ADH) with hyaluronic acid-aldehyde (HA-ALD), to provide a moist and inflammatory relief environment to promote stem cell proliferation or secretion of growth factors, thus accelerating wound healing. The results demonstrated that this injectable and self-healing hydrogel has excellent swelling properties, stability, and mechanical properties. This biocompatible hydrogel stimulated secretion of growth factors from bone marrow mesenchymal stem cells (BM-MSCs) and regulated the inflammatory environment by inhibiting the expression of M1 macrophages and promoting the expression of M2 macrophages, resulting in granulation tissue formation, collagen deposition, nucleated cell proliferation, neovascularization, and enhanced diabetic wound healing. This study showed that N-chitosan/HA-ALD hydrogel could be used as a multifunctional injectable wound dressing to regulate chronic inflammation and provide an optimal environment for BM-MSCs to promote diabetic wound healing.

16.
ACS Nano ; 14(6): 7552-7563, 2020 06 23.
Artigo em Inglês | MEDLINE | ID: mdl-32484332

RESUMO

It is undoubted the important role of cells in biology and medicine, but worldwide misidentified and cross-contaminated cell lines have caused much trouble in related fields. Herein, three kinds of supramolecular AIE (aggregation-induced emission) nanoassemblies were constructed by the host-guest interaction between tetraphenylethene (TPE) derivatives and cucurbit[8]uril (CB[8]). Based on the recognized mechanism of AIE, the TPE derivatives could achieve stronger fluorescence emission and higher fluorescence quantum yield after assembling with CB[8]. Moreover, the constructed supramolecular AIE complexes obtained well-confirmed nanostructures and exhibited different sizes and shapes. Correspondingly, they generated characteristic biological properties and fluorescence enhancement of cells. Inspired by the concept of Big Data Analysis, these fluorescence signals were further transformed into a unique fingerprint of cells via linear discriminant analysis. Immediately, we realized the veracious identification between a normal cell line, two cancer cell lines, and two metastasized cancer cell lines in a qualitative analysis. More importantly, it was well used to monitor the evaluation of cross-contaminated cells and the discrimination of cancer cells. As a proper bioapplication of ideal supramolecular nanomaterials, this system was easy to learn and apply, and the whole procedure was kept to 20 min, without cell disruption, centrifugation, or washing steps.


Assuntos
Autenticação de Linhagem Celular , Neoplasias
17.
Theranostics ; 10(11): 4779-4794, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32308749

RESUMO

Purpose: Osteoporosis is more likely to cause serious complications after joint replacement, mainly due to physiological defects of endogenous osteogenic cells and the pathological osteoclast activity. It is a feasible solution to design a prosthetic surface interface that specifically addresses this troublesome situation. Methods: A novel "three-dimensional (3D) inorganic-organic supramolecular bioactive interface" was constructed consisting of stiff 3D printing porous metal scaffold and soft multifunctional, self-healable, injectable, and biodegradable supramolecular polysaccharide hydrogel. Apart from mimicking the bone extracellular matrix, the bioactive interface could also encapsulate bioactive substances, namely bone marrow mesenchymal stem cells (BMSCs) and bone morphogenetic protein-2 (BMP-2). A series of in vitro characterizations, such as topography and mechanical characterization, in vitro release of BMP-2, biocompatibility analysis, and osteogenic induction of BMSCs were carried out. After that, the in vivo osseointegration effect of the bioactive interface was investigated in detail using an osteoporotic model. Results: The administration of injectable supramolecular hydrogel into the inner pores of 3D printing porous metal scaffold could obviously change the morphology of BMSCs and facilitate its cell proliferation. Meanwhile, BMP-2 was capable of being sustained released from supramolecular hydrogel, and subsequently induced osteogenic differentiation of BMSCs and promoted the integration of the metal microspores-bone interface in vitro and in vivo. Moreover, the osteoporosis condition of bone around the bioactive interface was significantly ameliorated. Conclusion: This study demonstrates that the 3D inorganic-organic supramolecular bioactive interface can serve as a novel artificial prosthesis interface for various osteogenesis-deficient patients, such as osteoporosis and rheumatoid arthritis.


Assuntos
Osso e Ossos/citologia , Hidrogéis/química , Células-Tronco Mesenquimais/metabolismo , Osteoporose/terapia , Tecidos Suporte/química , Animais , Proteína Morfogenética Óssea 2/metabolismo , Osso e Ossos/metabolismo , Células Cultivadas , Modelos Animais de Doenças , Matriz Extracelular , Feminino , Células-Tronco Mesenquimais/citologia , Osseointegração , Osteogênese , Osteoporose/metabolismo , Osteoporose/patologia , Impressão Tridimensional , Coelhos , Engenharia Tecidual/métodos
18.
ACS Appl Mater Interfaces ; 12(19): 21263-21269, 2020 May 13.
Artigo em Inglês | MEDLINE | ID: mdl-31825194

RESUMO

In this work, a new platform for pathogen discrimination and killing based on a conjugated polymer-quantum dot hybrid material was designed and constructed through the fluorescence resonance energy transfer (FRET) process. The hybrid material comprises water-soluble anionic CdSe/ZnS quantum dots (QDs) and a cationic poly(fluorene-alt-phenylene) derivative (PFP) through electrostatic interactions, thus promoting efficient FRET between PFP and QDs. Upon addition of different pathogen strains, the FRET from PFP to QDs was interrupted because of the competitive binding between PFP and the pathogens. Complexation of PFP and QDs also reduced the dark toxicity to a more desirable level, therefore potentially realizing the controllable killing of pathogens. The technique provides a promising theranostic platform in pathogen discrimination and disinfection based on FRET and phototoxicity of the PFP and QDs.


Assuntos
Antibacterianos/farmacologia , Antifúngicos/farmacologia , Desinfetantes/farmacologia , Corantes Fluorescentes/química , Polímeros/química , Pontos Quânticos/química , Antibacterianos/química , Antifúngicos/química , Compostos de Cádmio/química , Candida albicans/efeitos dos fármacos , Desinfetantes/química , Escherichia coli/efeitos dos fármacos , Fluorenos/química , Transferência Ressonante de Energia de Fluorescência , Microscopia Confocal , Microscopia de Fluorescência , Medicina de Precisão/métodos , Compostos de Selênio/química , Staphylococcus aureus/efeitos dos fármacos , Sulfetos/química , Compostos de Zinco/química
19.
Chem Sci ; 11(18): 4730-4740, 2020 Apr 14.
Artigo em Inglês | MEDLINE | ID: mdl-34122928

RESUMO

Visually identifying pathogens favors rapid diagnosis at the point-of-care testing level. Here, we developed a microenvironment-sensitive aggregation-induced emission luminogen (AIEgen), namely IQ-Cm, for achieving fast discrimination of Gram-negative bacteria, Gram-positive bacteria and fungi by the naked-eye. With a twisted donor-acceptor and multi-rotor structure, IQ-Cm shows twisted intramolecular charge transfer (TICT) and AIE properties with sensitive fluorescence color response to the microenvironment of pathogens. Driven by the intrinsic structural differences of pathogens, IQ-Cm with a cationic isoquinolinium moiety and a membrane-active coumarin unit as the targeting and interacting groups selectively locates in different sites of three pathogens and gives three naked-eye discernible emission colors. Gram-negative bacteria are weak pink, Gram-positive bacteria are orange-red and fungi are bright yellow. Therefore, based on their distinctive fluorescence response, IQ-Cm can directly discriminate the three pathogens at the cell level under a fluorescence microscope. Furthermore, we demonstrated the feasibility of IQ-Cm as a visual probe for fast diagnosis of urinary tract infections, timely monitoring of hospital-acquired infection processes and fast detection of molds in the food field. This simple visualization strategy based on one single AIEgen provides a promising platform for rapid pathogen detection and point-of-care diagnosis.

20.
Chem Commun (Camb) ; 55(96): 14466-14469, 2019 Nov 28.
Artigo em Inglês | MEDLINE | ID: mdl-31728462

RESUMO

We report a supramolecular photo-responsive antibiotic (azobenzene-norfloxacin/αCD). This supramolecule exhibited a higher "on-off" ratio of antibacterial ability than azobenzene-norfloxacin alone under UV irradiation. It offers an approach to efficiently regulate the activity of antibiotics by combining the supramolecular and light-regulating strategies together.


Assuntos
Antibacterianos/química , Compostos Azo/química , beta-Ciclodextrinas/química , Antibacterianos/farmacologia , Bactérias/efeitos dos fármacos , Farmacorresistência Bacteriana/efeitos dos fármacos , Isomerismo , Norfloxacino/química , Norfloxacino/farmacologia , Temperatura , Raios Ultravioleta
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