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1.
Bioengineered ; 2021 Oct 12.
Artigo em Inglês | MEDLINE | ID: mdl-34637696

RESUMO

This research was to explore the antibiotic-induced drug resistance of Salmonella enteritidis and its biofilm formation mechanism. Kirby-Bauer (K-B) disk method recommended by Clinical and Laboratory Standards Institute (CLSI) was used to test the drug sensitivity of Salmonella enteritidis to 16 kinds of antibiotics including ß-lactams, aminoglycosides, quinolones, sulfonamides, chloramphenicols, and tetracyclines. Polymerase chain reaction (PCR) was performed to detect the carrying of drug resistance genes of 29 kinds of antibiotics including ß-lactams, aminoglycosides, quinolones, sulfonamides, chloramphenicols, and tetracyclines of Salmonella enteritidis. The expressions of esp, ebpA, ge1E, and fsrB genes in biofilm group and plankton group were detected when Salmonella was induced, and the difference of gene expression before and after induction was detected by FQ-PCR. The drug resistance rates of Salmonella enteritidis to nalidixic acid, ampicillin, streptomyces, and cefoperazone were high, which were 94.5%, 75%, 67%, and 52%, respectively. 94 strains of Salmonella enteritidis formed 22 kinds of drug resistance spectrum, the strains were generally resistant to 4-5 antibiotics, and some strains formed fixed drug resistance spectrum as follows: AMP-CFP-STR-NA-TE (22.6,21.7%), AMP-STR-NA-TE (17,16%), and AMP-CFP-STR-NA (11.1,10.6%). During biofilm formation, fsr can increase the expression of ge1E and decrease the expression of esp and ebpA. In summary, Salmonella enteritidis was generally resistant to nalidixic acid, ampicillin, and streptomycin, and the multidrug resistance was severe. The drug resistance genes sul2, sul3, blaTEM-1-like, tet(A), and tet(G) were highly carried in Salmonella enteritidis. In addition, esp, ebpA, ge1E, and fsrB genes were closely related to the biofilm formation of Salmonella enteritidis.

2.
Environ Sci Technol ; 2021 Oct 12.
Artigo em Inglês | MEDLINE | ID: mdl-34634907

RESUMO

H2S and CO2 are the main impurities in raw natural gas, which needs to be purified before use. However, the comprehensive utilization of H2S and CO2 has been ignored. Herein, we proposed a fully resource-based method to convert toxic gas H2S and greenhouse gas CO2 synchronously into CO and elemental S by using a novel electrochemical reactor. The special designs include that, in the anodic chamber, H2S was oxidized rapidly to S based on the I-/I3- cyclic redox system to avoid anode passivation. On the other hand, in the cathodic chamber, CO2 was rapidly and selectively reduced to CO based on a porous carbon gas diffusion electrode (GDE) modified with polytetrafluoroethylene and cobalt phthalocyanine (CoPc). A high Faraday efficiency (>95%) toward CO was achieved due to the enhanced mass transfer of CO2 on the GDE and the presence of the selective CoPc catalyst. The maximum energy efficiency of the system was more than 72.41% with a current density of over 50 mA/cm2, which was 12.5 times higher than what was previously reported on the H2S treatment system. The yields of S and CO were 24.94 mg·cm-2·h-1 and 19.93 mL·cm-2·h-1, respectively. A model analysis determined that the operation cost of the synchronous utilization of H2S and CO2 method was slightly lower than that of the single utilization of H2S in the existing natural gas purification technology. Overall, this paper provides efficient and simultaneous conversion of H2S and CO2 into S and CO.

3.
Artigo em Inglês | MEDLINE | ID: mdl-34622375

RESUMO

Incomplete partition type II (IP-II) is frequently identified in ears with SLC26A4 mutations. Cochleae with IP-II are generally observed to have 1½ turns; the basal turns are normally formed, and the apical turn is dilated or cystic. The objective of this study was to characterize the pathomorphogenesis of the IP-II cochlear anomaly in Slc26a4-null mice. Otic capsules were dissected from Slc26a4Δ/+ and Slc26a4Δ/Δ mice at 1 and 8 days of age and at 1 and 3 months of age. X-ray micro-computed tomography was used to image samples. We used a multiplanar view and three-dimensional reconstructed models to calculate the cochlear duct length, cochlear turn rotation angle, and modiolus tilt angle. The number of inner hair cells was counted, and the length of the cochlear duct was measured in a whole-mount preparation of the membranous labyrinth. X-ray micro-computed tomography mid-modiolar planar views demonstrated cystic apical turns in Slc26a4Δ/Δ mice resulting from the loss or deossification of the interscalar septum, which morphologically resembles IP-II in humans. Planes vertical to the modiolus showed a similar mean rotation angle between Slc26a4Δ/+ and Slc26a4Δ/Δ mice. In contrast, the mean cochlear duct length and mean number of inner hair cells in Slc26a4Δ/Δ mice were significantly smaller than in Slc26a4Δ/+ mice. In addition, there were significant differences in the mean tilt angle and mean width of the modiolus. Our analysis of Slc26a4-null mice suggests that IP-II in humans reflects loss or deossification of the interscalar septum but not a decreased number of cochlear turns.

4.
Sci Rep ; 11(1): 19950, 2021 Oct 07.
Artigo em Inglês | MEDLINE | ID: mdl-34620949

RESUMO

In an image based virtual try-on network, both features of the target clothes and the input human body should be preserved. However, current techniques failed to solve the problems of blurriness on complex clothes details and artifacts on human body occlusion regions at the same time. To tackle this issue, we propose a non-local virtual try-on network NL-VTON. Considering that convolution is a local operation and limited by its convolution kernel size and rectangular receptive field, which is unsuitable for large size non-rigid transformations of persons and clothes in virtual try-on, we introduce a non-local feature attention module and a grid regularization loss so as to capture detailed features of complex clothes, and design a human body segmentation prediction network to further alleviate the artifacts on occlusion regions. The quantitative and qualitative experiments based on the Zalando dataset demonstrate that our proposed method significantly improves the ability to preserve features of bodies and clothes compared with the state-of-the-art methods.

5.
IEEE Trans Cybern ; PP2021 Oct 04.
Artigo em Inglês | MEDLINE | ID: mdl-34606468

RESUMO

Convolutional neural networks (CNNs) have achieved remarkable performance in driver drowsiness detection based on the extraction of deep features of drivers' faces. However, the performance of driver drowsiness detection methods decreases sharply when complications, such as illumination changes in the cab, occlusions and shadows on the driver's face, and variations in the driver's head pose, occur. In addition, current driver drowsiness detection methods are not capable of distinguishing between driver states, such as talking versus yawning or blinking versus closing eyes. Therefore, technical challenges remain in driver drowsiness detection. In this article, we propose a novel and robust two-stream spatial-temporal graph convolutional network (2s-STGCN) for driver drowsiness detection to solve the above-mentioned challenges. To take advantage of the spatial and temporal features of the input data, we use a facial landmark detection method to extract the driver's facial landmarks from real-time videos and then obtain the driver drowsiness detection result by 2s-STGCN. Unlike existing methods, our proposed method uses videos rather than consecutive video frames as processing units. This is the first effort to exploit these processing units in the field of driver drowsiness detection. Moreover, the two-stream framework not only models both the spatial and temporal features but also models both the first-order and second-order information simultaneously, thereby notably improving driver drowsiness detection. Extensive experiments have been performed on the yawn detection dataset (YawDD) and the National TsingHua University drowsy driver detection (NTHU-DDD) dataset. The experimental results validate the feasibility of the proposed method. This method achieves an average accuracy of 93.4% on the YawDD dataset and an average accuracy of 92.7% on the evaluation set of the NTHU-DDD dataset.

6.
Lung Cancer ; 162: 16-22, 2021 Sep 28.
Artigo em Inglês | MEDLINE | ID: mdl-34649105

RESUMO

BACKGROUND: Early detection of lung cancer in asymptomatic patients remains challenging, especially for stage I. Considering the substantial interaction with tumor immunogenicity, we hypothesized that lung cancer-associated TCR (LC-aTCR) may serve as potential biomarker in early detection of stage I lung cancer. METHODS: Individuals who received low-dose computed tomography (LDCT) screening were enrolled in the study. Surgical tissues and peripheral blood specimens were collected and performed with DNA-based T cell repertoire (TCR) sequencing. The motif-based algorithm was used to deconstruct specific lung cancer-associated TCRs (LC-aTCRs). RESULTS: A total of 146 individuals participating in the real-world LDCT screening project were enrolled in this study, including 52 patients with pathologically-confirmed stage I lung cancer and 94 non-cancer controls. We developed a motif-based algorithm to define 80 LC-aTCRs in the training cohort. Moreover, in the validation cohort, high sensitivity and specificity was showed in stage I lung cancer with 72% and 91% respectively, and the AUC of the ROC curve was 0.91 (95% CI: 0.85 ∼ 0.96). CONCLUSION: This work provides inspiration for stage I lung cancer detection by using blood TCR profiling data. The combination of TCR-based assay and routine screening deserves further testing in larger cohorts.

7.
Zhongguo Zhong Yao Za Zhi ; 46(12): 3106-3115, 2021 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-34467702

RESUMO

To obtain the difference of the fungal and bacterial community diversity between wild Cordyceps sinensis, artificial C. sinensis and their habitat soil, Illmina Hiseq high-throughput sequencing technology was applied. The results show that Proteobacteria was the dominant bacterial phylum in C. sinensis, Actinobacteria was the dominant bacterial phylum in soil microhabitat, Ophiocordyceps sinensis was the predominant dominant fungus of C. sinensis. The α diversity analysis showed that the fungal diversity of stroma was lower than other parts, and the fungal diversity of wild C. sinensis was lower than that of artificial C. sinensis. The ß diversity analysis showed that the fungal and bacterial community diversity of soil microhabitat samples was significantly different from that of C. sinensis. The fungal community diversity was less different between wild and artificial C. sinensis, especially in sclerotia. LEfSe analysis showed a lot of species diversity between wild and artificial C. sinensis. Those different species between wild C. sinensis, artificial C. sinensis and their habitat soil provide ideas for further research on breed and components of C. sinensis.


Assuntos
Cordyceps , Microbiota , Cordyceps/genética , Sequenciamento de Nucleotídeos em Larga Escala , Microbiota/genética , Solo , Microbiologia do Solo
8.
Artigo em Inglês | MEDLINE | ID: mdl-34473939

RESUMO

RATIONALE: Ground-glass opacity (GGO)-associated lung cancers are common and radiologically distinct clinical entities known to have indolent clinical course and superior survival implying a unique underlying biology. However, the molecular and immune characteristics of GGO-associated lung nodules have not been systemically studied. Objectives Providing mechanistic insights for the treatment of these radiologically distinct clinical entities. Methods We initiated a prospective cohort study to collect and characterize pulmonary nodules with (non-solid and part-solid) or without GGO components precisely quantified by 3D image reconstruction to delineate molecular and immune features associated with GGO. Multiomics assessment by targeted gene panel sequencing, RNA sequencing, T cell receptor sequencing and circulating tumor DNA detection was performed. Measurements and Main Results: GGO-associated lung cancers exhibited lower tumor mutation burden than solid nodules. Transcriptomic analysis revealed a less active immune environment in GGO and immune pathways, decreased expression of immune activation markers and lower infiltration of most immune cell subsets, which was confirmed by multiplex immunofluorescence. Furthermore, T cell repertoire (TCR) sequencing revealed lower T cell expansion in GGO-associated lung cancers. Loss of heterozygosity of HLA was significantly less common in lung adenocarcinomas with GGO components than those without. Circulating tumor DNA analysis suggested that release of tumor DNA to peripheral blood was correlated with tumor size of non-GGO components. Conclusions Compared to lung cancers presenting with solid lung nodules, GGO-associated lung cancers are characterized by less active metabolism and immune microenvironment that may be the mechanisms underlying their indolent clinical course.

9.
mSystems ; : e0080721, 2021 Sep 07.
Artigo em Inglês | MEDLINE | ID: mdl-34491088

RESUMO

Nitrogen in different chemical forms is critical for metabolic alterations in Monascus strains and associated pigment diversity. In this study, we observed that ammonium-form nitrogen was superior in promoting the biosynthesis of Monascus pigments (MPs) when compared with nitrate and organic forms. Moreover, with any nitrogen source, the production of yellow and orange pigments was highly synchronized but distantly related to red pigments. However, transcriptional analyses of MP gene clusters suggested a low contribution to MP accumulation, suggesting that MP-limiting factors were located outside the gene cluster. Our metabolomic analyses demonstrated that red pigment biosynthesis was closely related to intracellular amino acids, whereas orange and yellow pigments were associated with nucleotides. In addition, weighted gene coexpression network analyses (WGCNA) based on transcriptomic data showed that multiple primary metabolic pathways were closely related to red pigment production, while several secondary pathways were related to orange pigments, and others were involved with yellow pigment regulation. These findings demonstrate that pigment diversity in Monascus is under combined regulation at metabolomic and transcriptomic levels. IMPORTANCE Natural MPs containing a mixture of red, orange, and yellow pigments are widely used as food coloring agents. MP diversity provides foods with versatile colors and health benefits but, in turn, complicate efforts to achieve maximum yield or desirable combination of pigments during the manufacturing process. Apart from the MP biosynthetic gene cluster, interactions between the main biosynthetic pathways and other intracellular genes/metabolites are critical to our understanding of MP differentiation. The integrative multiomics analytical strategy provides a technical platform and new perspectives for the identification of metabolic shunting mechanisms in MP biosynthesis. Equally, our research highlights the influence of intracellular metabolic alterations on MP differentiation, which will facilitate the rational engineering and optimization of MP production in the future.

10.
BMC Gastroenterol ; 21(1): 354, 2021 Sep 27.
Artigo em Inglês | MEDLINE | ID: mdl-34579655

RESUMO

BACKGROUND: DAL-1 gene was reported to inhibit proliferation, migration, invasion, and epithelial to mesenchymal transition (EMT) of gastric cancer (GC) cells in our previous study. The association between the genomic variants in DAL-1 gene with risk of GC is still unclear. METHODS: In this study, 505 GC cases and 544 healthy controls (HCs) were collected to evaluate the association between six single nucleotide polymorphisms (SNPs) (rs7240736, rs73937194, rs3817466, rs8082898, rs73381527, rs9953490) of DAL-1 gene and GC risk in the Han population in Northeast China. RESULTS: The TA + AA genotypes of rs9953490 were significantly associated with an increased risk in N3 compared with N0 subgroup (adjusted OR = 4.56, 95% CI = 1.49-13.98, P = 0.008), and also showed evident association with an increased risk in TNM stage III compared with stage I-II (adjusted OR = 2.33, 95% CI = 1.16-4.67, P = 0.017). CONCLUSION: The rs9953490 of DAL-1 gene may play an important role in the occurrence and development of GC in the Han population in Northeast China.


Assuntos
Neoplasias Gástricas , Grupo com Ancestrais do Continente Asiático/genética , Estudos de Casos e Controles , China , Transição Epitelial-Mesenquimal , Estudos de Associação Genética , Predisposição Genética para Doença , Genótipo , Humanos , Proteínas dos Microfilamentos , Polimorfismo de Nucleotídeo Único , Neoplasias Gástricas/genética
11.
Br J Nutr ; : 1-11, 2021 Sep 02.
Artigo em Inglês | MEDLINE | ID: mdl-34470677

RESUMO

Little is known about the impact of modifiable risk factors on blood pressure (BP) trajectories and their associations with hypertension (HTN). We aimed to identify BP trajectories in normotensive Chinese adults and explore their influencing factors and associations with HTN. We used data from 3436 adults with at least four BP measurements between 1989 and 2018 in the China Health and Nutrition Survey, an ongoing cohort study. We measured BP using mercury sphygmomanometers with appropriate cuff sizes in all surveys. We used group-based trajectory modelling to identify BP trajectories between 1989 and 2009 and multiple logistic and Cox regression models to analyse their influencing factors and associations with HTN in 2011-2018. We identified five systolic blood pressure (SBP) trajectories, 'Low-increasing (LI)', 'Low-stable (LS)', 'Moderate-increasing (MI)', 'High-stable (HS)' and 'Moderate-decreasing (MD)', and four diastolic blood pressure (DBP) trajectories classified as 'Low-increasing (LI)', 'Moderate-stable (MS)', 'Low-stable (LS)' and 'High-increasing (HI)'. People with higher physical activity (PA) levels and lower waist circumferences (WC) were less likely to be in the SBP LI, MI, HS and MD groups (P < 0·05). People with higher fruit and vegetable intakes, lower WCs and salt intakes and higher PA levels were less likely to be in the DBP LI, MS and HI groups (P < 0·05). Participants in the SBP HS group (hazard ratio (HR) 2·01) or the DBP LI, MS and HI groups (HR 1·38, 1·40, 1·71, respectively) had higher risks of HTN (P < 0·05). This study suggests that BP monitoring is necessary to prevent HTN in the Chinese population.

12.
Mater Sci Eng C Mater Biol Appl ; 129: 112372, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34579891

RESUMO

3D-printed scaffolds have been developed as potential therapeutic strategies in bone tissue engineering. Mg/PCL biomaterials have been attracted much attention owing to biocompatibility, biodegradability as well as tunable mechanical properties. In this work, we developed 3D-printed customized Mg/PCL composite scaffolds with enhanced osteogenesis and biomineralization. Mg microparticles embedded in PCL-based scaffolds took a positive role in the improvement of biocompatibility, biomineralization, and biodegradable abilities. When incorporated with 3 wt% Mg, PCL-based scaffolds exhibited the optimal bone repairing ability in vitro and in vivo. The in vitro experiments indicated that 3 Mg/PCL scaffolds had improved mechanical properties, good biocompatibility, enhanced osteogenic and angiogenic activities. Besides, the in vivo studies demonstrated that Mg/PCL scaffolds promoted tissue ingrowth and new bone formation. In sum, these findings indicated that 3D-printed cell-free Mg/PCL scaffolds are promising strategies for bone healing application.


Assuntos
Impressão Tridimensional , Tecidos Suporte , Materiais Biocompatíveis/farmacologia , Regeneração Óssea , Osteogênese , Poliésteres , Engenharia Tecidual
13.
IEEE Trans Cybern ; PP2021 Aug 31.
Artigo em Inglês | MEDLINE | ID: mdl-34464282

RESUMO

Achieving reliable intervehicle positioning is one of the most fundamental elements for many vehicular applications, including collision avoidance and autonomous driving. Vehicle position is generally provided by a global navigation satellite system (GNSS), which unfortunately suffers from inaccuracy to varying degrees in challenging environments, for example, GNSS outages. In this article, a reliable fusion technique, called non-Gaussian Redheffer weighted least squares (nGRWLSs), is proposed. This new approach highlights the intervehicle positioning estimation in multi-GNSS outage environments, such as complete, partial, and free GNSS pseudorange outages. The proposed method combines, on the one hand, the benefits of the Gaussian dynamical matrix principle and the Redheffer distribution function for the sparse property in complete GNSS pseudorange outages and, on the other hand, the use of the optimal window size to regulate the data flow generated by both the inertial navigation systems (INSs) and GNSS during a partial GNSS pseudorange outage. During the free GNSS pseudorange outage, the process ignores data from the INS, and instead, GNSS pseudorange information alone will be considered to compute the intervehicle positioning information. Consequently, weighted least squares is used as an intervehicle positioning estimator. To address the pseudorange uncommon and INS measurement noises, the generalized error distribution (GED) is used to estimate the non-Gaussian densities. Finally, road-test experiments are implemented to evaluate the consistency of the proposed approach. The experimental results show that the proposed nGRWLS can accurately estimate the intervehicle positioning under various conditions (free, partial, and complete GNSS pseudorange outages).

14.
J Tradit Chin Med ; 41(4): 507-514, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-34392642

RESUMO

OBJECTIVE: To use evidence-based medicine to systematically evaluate the effectiveness and safety of acupuncture therapy for improving anorexia in tumor patients. METHODS: We queried the China National Knowledge Infrastructure Database (CNKI), China Science and Technology Journal Database (VIP), Wanfang Data, PubMed, Cochrane Library, and Embase databases to identify reports of randomized controlled trials (RCTs) that applied acupuncture therapy to improve anorexia in tumor patients, and used Rev Man 5.3 software to conduct a Meta-analysis of the effective rate, appetite score, Karnofsky Performance Status (KPS) score, Functional Assessment of Anorexia/Cachexia Therapy (FAACT) appetite scale, and body weight in each study. Subgroup analysis was conducted based on whether radiotherapy or chemotherapy were also administered. RESULTS: A total of 10 RCTs were included with a total of 648 patients, including 343 patients in the treatment group and 305 patients in the control group. The Meta-analysis results showed that the clinical efficacy, appetite score, KPS score, and FAACT score of the treatment group (which received acupuncture to improve appetite) were better than those of the control group, and the difference was statistically significant (P < 0.05); however, there was no statistically significant difference in body weight between the treatment group and the control group (P > 0.05). The results of the subgroup analysis showed that the effective rate and appetite score for patients with long-term and chronic loss of appetite who underwent acupuncture were better than those of the control group, and the difference was statistically significant (P < 0.05). CONCLUSIONS: Acupuncture therapy has good efficacy and safety in the treatment of anorexia in tumor patients, and it also has good efficacy and safety for long-term and chronic loss of appetite. The reliability and stability of the above results need to be confirmed by high-quality RCTs with larger sample sizes.

15.
J Genet Genomics ; 2021 Jul 06.
Artigo em Inglês | MEDLINE | ID: mdl-34393089

RESUMO

Exposure to intense noise can damage cochlear hair cells, leading to hearing loss in mammals. To avoid this constraint, most mammals have evolved in relatively quiet environments. Echolocating bats, however, are naturally exposed to continuous intense sounds from their own and neighboring sonar emissions for maintaining sonar directionality and range. Here, we propose the presence of intense noise resistance in cochlear hair cells of echolocating bats against noise-induced hearing loss (NIHL). To test this hypothesis, we performed noise exposure experiments for laboratory mice, one nonecholocating bat species, and five echolocating bat species. Contrary to nonecholocating fruit bats and mice, the hearing and the cochlear hair cells of echolocating bats remained unimpaired after continuous intense noise exposure. The comparative analyses of cochleae transcriptomic data showed that several genes protecting cochlear hair cells from intense sounds were overexpressed in echolocating bats. Particularly, the experimental examinations revealed that ISL1 overexpression significantly improved the survival of cochlear hair cells. Our findings support the existence of protective effects in cochlear hair cells of echolocating bats against intense noises, which provides new insight into understanding the relationship between cochlear hair cells and intense noises, and preventing or ameliorating NIHL in mammals.

16.
ACS Nano ; 2021 Aug 12.
Artigo em Inglês | MEDLINE | ID: mdl-34383483

RESUMO

Plasmonic chiral metamaterials have attracted broad research interest because of their potential applications in optical communication, biomedical diagnosis, polarization imaging, and circular dichroism spectroscopy. However, optical losses in plasmonic structures severely limit practical applications. Here, we present the design concept and experimental demonstration for highly efficient subwavelength-thick plasmonic chiral metamaterials with strong chirality. The proposed designs utilize plasmonic metasurfaces to control the phase and polarization of light and exploit anisotropic thin-film interference effects to enhance optical chirality while minimizing optical loss. Based on such design concepts, we demonstrated experimentally optical devices such as circular polarization filters with transmission efficiency up to 90% and extinction ratio >180, polarization converters with conversion efficiency up to 90%, as well as on-chip integrated microfilter arrays for full Stokes polarization detection with high accuracy over a broad wavelength range (3.5-5 µm). The proposed design concepts are applicable from near-infrared to Terahertz regions via structural engineering.

17.
J Hazard Mater ; 422: 126829, 2021 Aug 06.
Artigo em Inglês | MEDLINE | ID: mdl-34399228

RESUMO

Levofloxacin (LEV) infiltrated in groundwater has threatened the safety of drinking water. For in-situ remediation of LEV-contaminated groundwater, there exists a main challenge of exploiting proper high efficient backfill medium in utilizing charming permeable reactive barriers (PRBs). Herein, three porous shapeable three-component hydrogen-bonded covalent organic aerogels (HCOA-1, HCOA-2 and HCOA-3) were fabricated based on a multiple-linking-site strategy to evaluate for adsorptive removal of LEV. The three HCOAs exhibited satisfactory performance in LEV adsorption that could integrate high adsorption capacity, good antiion interference, excellent recyclability and wide pH tolerance. The different regularity of kinetics and isotherms of three HCOAs signified that electrostatic effect, pore preservation, hydrogen bonding probably govern the adsorption process in combination, coupling with π-π electron-donor-acceptor (EDA), dipole-dipole and hydrophobic-hydrophobic interaction besides. In addition, the response surface methodology (RSM) was employed for studying the single and synergetic effects of selected variables and optimizing operation conditions. Furthermore, a laboratory PRB column packed with processable HCOA-2 was set up to investigate the LEV removal, and the breakthrough data was explained by Adams-Bohart, Thomas, BDST and Yoon-Nelson models. We believe could hopefully bring HCOAs into the real in-situ remediation of such challenging and persistent LEV-polluted groundwater with further massive-scale efficiently.

18.
J Environ Manage ; 297: 113415, 2021 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-34333310

RESUMO

In this study, the biodegradation of polycyclic aromatic hydrocarbons (PAHs) in soil washing solution containing Tween 80 was conducted using native mixed bacteria (Pseudomonas sp. Z1, Sphingobacterium sp. Z2, and Klebsiella sp. K) embedded in polyvinyl alcohol-sodium alginate-nano alumina (PVA-SA-ALNPs) gel beads. The optimal dosage of immobilized beads and embedded biomass for the biodegradation of phenanthrene (PHE), fluoranthene (FLU), and pyrene (PYR) were 10 % (v/v) and 20 % (v/v), respectively. SEM analysis showed that the porous structure of the immobilized beads was a cross-linked network with abundant pores that provided many potential adhesion sites for microorganisms. The beads with the immobilized mixed bacteria maintained a high activity during batch experiments and could even be reused for 3 cycles (90 d). Compared with the beads containing individual immobilized strain, the immobilized mixed bacteria showed a more efficient biodegradation of PHE (91.67 %), FLU (88.6 %), and PYR (88.5 %) in synthetic soil washing effluent within 30 d. The first-order kinetic model suitably described the degradation process of the three target PAHs. By adding Tween 80 to the synthetic eluent, the degradation of PHE, FLU, and PYR increased by 16.39 %, 22.25 %, and 21.29 %, respectively, indicating that Tween 80 promoted PAHs biodegradation, even though it was also rapidly degraded during the reaction cycle. These findings suggest that the developed mixed bacteria embedded in PVA-SA-ALNPs gel beads has great potential for PAHs remediation.


Assuntos
Hidrocarbonetos Policíclicos Aromáticos , Poluentes do Solo , Alginatos , Óxido de Alumínio , Bactérias , Biodegradação Ambiental , Álcool de Polivinil , Solo
19.
ACS Appl Mater Interfaces ; 13(33): 39055-39065, 2021 Aug 25.
Artigo em Inglês | MEDLINE | ID: mdl-34433248

RESUMO

Polymer photosensitizers (PPSs) with the distinctive properties of good light-harvesting capability, high photostability, and excellent tumor retention effects have aroused great research interest in photodynamic therapy (PDT). However, their potential translation into clinic was often constrained by the hypoxic nature of tumor microenvironment, the aggregation-caused reduced production of reactive oxygen species (ROS), and the tedious procedure of manufacture. As a powerful and versatile strategy, vacancy engineering possesses the unique capability to effectively improve the photogenerated electron efficiency of nanomaterials for high-performance O2 and ROS production. Herein, by introducing vacancy engineering into the design of PPSs for PDT for the first time, we synthesized a novel PPS of Au-decorated polythionine (PTh) nanoconstructs (PTh@Au NCs) with the unique integrated features of distinguished O2 self-evolving function and highly efficient ROS generation for achieving the greatly enhanced PDT efficacy toward hypoxic tumor both in vitro and in vivo. The incorporation of Au into PTh leads to the special PTh-Au heterostructure-induced sulfur vacancies in PTh@Au NCs, which results in an efficient electron-hole separation performance and also plays a key role in a long lifetime of free electrons and holes. Accordingly, an ∼2- to 3-fold ROS generation and an ∼1.5-fold increase of O2 self-supply than the pure PTh nanoparticles (NPs) were obtained even under hypoxic conditions upon exposure to 650 nm light. By combining such superior ROS generation and O2 self-supply performances with the outstanding cellular internalization and tumor accumulation capacities, an advanced antitumor effect with the achievement of almost complete hypoxic tumor elimination in vivo or 88% cell destruction in vitro was acquired by the PTh@Au NCs. In addition, the distinctive facile one-step redox strategy for PTh@Au NCs synthesis compared to the reported PPSs for PDT also makes it beneficial for potential practical application. The first introduction of vacancy engineering concept into PPSs in the field of PDT proposed in this work offers a new strategy for the development and design highly efficient PPSs for PDT applications.


Assuntos
Antineoplásicos/química , Ouro/química , Nanopartículas Metálicas/química , Fenotiazinas/química , Fármacos Fotossensibilizantes/química , Polímeros/química , Animais , Antineoplásicos/farmacocinética , Linhagem Celular Tumoral , Permeabilidade da Membrana Celular , Feminino , Humanos , Hipóxia/metabolismo , Camundongos , Neoplasias Experimentais , Oxigênio/metabolismo , Fenotiazinas/farmacocinética , Fotoquimioterapia , Fármacos Fotossensibilizantes/farmacocinética , Polímeros/farmacocinética , Espécies Reativas de Oxigênio/metabolismo , Nanomedicina Teranóstica , Microambiente Tumoral
20.
Cancer Sci ; 2021 Aug 21.
Artigo em Inglês | MEDLINE | ID: mdl-34418232

RESUMO

The purpose of this study was to investigate the pathogenesis of colorectal cancer (CRC) and the effects of the long non-coding RNA (lncRNA) plasmacytoma variant translocation 1 (PVT1) on CRC progression. Bioinformatics analysis verified PVT1 expression in tumor and normal tissues. qPCR and Western blotting were used to measure mRNA and protein levels, respectively. MTT, transwell, colony formation, and in vivo assays were used to assess the effects of PVT1 on proliferation, migration, and invasion by CRC cells. PVT1 and miR-152-3p were shown to be colocalized in CRC cells using FISH assay. The target genes of miR-152-3p were predicted and verified by bioinformatics analysis, luciferase assay and RNA pull-down assay. ChIP assay revealed that E2F3 binds with the promoter of MAPK8. PVT1 was overexpressed in CRC specimens, and its expression was higher in CRC cells than normal intestinal cells. PVT1 overexpression enhanced the proliferation, migration, and invasion of CRC cells, while PVT1 knockdown inhibited these processes. miR-152-3p was a target of PVT1, and E2F3 was a target of miR-152-3p. Rescue experiments confirmed the interaction between miR-152-3p and PVT1 and between miR-152-3p and E2F3. Luciferase and ChIP assay results confirmed that E2F3 modulates the transcriptional activation of MAPK8. PVT1 activated E2F3 signaling by sponging miR-152-3p. The PVT1/miR-152-3p/E2F3/MAPK8 axis promoted CRC progression.

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