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1.
J Eur Acad Dermatol Venereol ; 33(2): 410-415, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30169916

RESUMO

BACKGROUND: Pemphigus is an autoimmune blistering disease with pemphigus vulgaris (PV) and foliaceus (PF) as the two major histological subtypes. Associations with HLA molecules have been suggested, but specific HLA risk variants as well as non-HLA risk variants remain to be discovered. METHODS: We performed a two-stage genome-wide association study in the Chinese Han population through a genome-wide discovery analysis and follow-up validation analysis in a total number of 210 PV, 159 PF and 2493 healthy controls. HLA imputation as well as high coverage next generation sequencing based HLA genotyping was employed to investigate the association of classical HLA alleles and amino acid change. RESULTS: We have discovered independent novel associations with PF at rs2178077 on 12q24.33, located next to RAN (PPF = 1.57 × 10-9 ) and rs3888722 within the MHC region (P = 6.73 × 10-9 ). For the HLA variants, we confirmed independent genome-wide level risk associations in HLA-DQB1 and HLA-DRB1, with DQB1*05:03 to be the strongest association with PV (P = 8.59 × 10-68 , OR = 31.16) and PF (P = 4.84 × 10-17 , OR = 5.64). In addition, DRB1*14 was demonstrated to be a second independent variants (P = 4.2 × 10-63 , OR = 35.47) for PV, while DRB1*04:06 was demonstrated to be the second independent signal (P = 7.44 × 10-13 , OR = 5.58) for PF. CONCLUSIONS: These findings advance our understanding of the genetic basis of pemphigus susceptibility and may offer opportunities for risk prediction and preventive treatment for pemphigus, in particular for PV.


Assuntos
Predisposição Genética para Doença/epidemiologia , Sequenciamento de Nucleotídeos em Larga Escala/métodos , Pênfigo/epidemiologia , Pênfigo/genética , Grupo com Ancestrais do Continente Asiático/genética , Estudos de Casos e Controles , Causalidade , China/epidemiologia , Feminino , Frequência do Gene , Estudo de Associação Genômica Ampla , Cadeias beta de HLA-DQ/genética , Cadeias HLA-DRB1/genética , Humanos , Masculino , Pênfigo/patologia , Prevalência , Reprodutibilidade dos Testes
2.
J Neuroimaging ; 28(6): 650-655, 2018 11.
Artigo em Inglês | MEDLINE | ID: mdl-30039613

RESUMO

BACKGROUND AND PURPOSE: Previous cross-sectional studies have reported the involvement of thalamus, pallidum, superior cerebellar peduncle, temporal cortex, and fronto-parietal white matter in multiple sclerosis (MS)-related fatigue. However, the longitudinal imaging correlates of fatigue severity variation in MS remain unclear. METHODS: Structural and diffusion tensor imaging (DTI) data were collected from 43 relapsing remitting MS (RRMS) patients with fatigue (Fatigue Severity Scale [FSS] range: 1-7). Subcortical nuclei volume using FreeSurfer and cortical diffusion indices using a cross-modality technique were measured at baseline and year 1. RESULTS: The pallidal volume had significantly decreased (P = .002) by year 1, although the fatigue score variation was not significant. At year 1, the pallidal volume (P = .023) and fractional anisotropy (FA, P = .013) of right temporal cortex (RTC) correlated significantly with FSS. CONCLUSIONS: The pallidal volume and the FA-RTC may be used to evaluate longitudinal fatigue severity variation. Our study proposes new biomarkers to monitor fatigue severity in MS patients.


Assuntos
Córtex Cerebral/diagnóstico por imagem , Imagem de Tensor de Difusão/métodos , Fadiga/diagnóstico por imagem , Globo Pálido/diagnóstico por imagem , Imagem por Ressonância Magnética/métodos , Esclerose Múltipla Recidivante-Remitente/diagnóstico por imagem , Substância Branca/diagnóstico por imagem , Adulto , Estudos Transversais , Humanos , Pessoa de Meia-Idade , Estudos Retrospectivos , Adulto Jovem
3.
Zhonghua Xue Ye Xue Za Zhi ; 39(6): 454-459, 2018 Jun 14.
Artigo em Chinês | MEDLINE | ID: mdl-30032559

RESUMO

Objective: To investigate the outcomes of anti-CD19 CAR-T cell for relapsed and refractory B cell malignancies. Method: Ten patients with relapsed and refractory B cell acute lymphocytic leukemia (B-ALL) and non-Hodgkin's lymphoma (NHL), diagnosed in the Department of Hematology of Peking University third Hospital from December 2015 to July 2017, were treated with anti-CD19 CAR-T cell therapy, and the efficacy and safety were analyzed. Results: Efficacy was assessed on the 28th day after cell infusion, including 66.7% (4/6) of complete remission (CR) for patients with ALL, 16.7% (1/6) of partial remission (PR), and 83.3% (5/6) of overall response rate (ORR). For NHL patients, CR was 33.3% (1/3) and most of the lesions disappeared in a patient with mantle cell lymphoma, but residual lesion presented persistent state. After infusion of anti-CD19 CAR-T cells, the main side effect was cytokine release syndrome (CRS) and fever. One patient presented with aphasia and the other one had multiple organ failure, which were improved after treatment. No patients died of CRS. Conclusion: anti-CD19 CAR-T cell for relapsed and refractory B cells hematological malignancies is safe, and the most problematic side effect is CRS, which can be controlled by therapy.


Assuntos
Leucemia de Células B , Linfócitos T , Antígenos CD19 , Linfócitos B , Terapia Baseada em Transplante de Células e Tecidos , Humanos , Receptores de Antígenos de Linfócitos T , Recidiva
4.
Clin Genet ; 94(2): 259-263, 2018 08.
Artigo em Inglês | MEDLINE | ID: mdl-29722023

RESUMO

Seven new risk coding variants have been identified through an exome-wide association study (EWAS), which studied the contributions of protein-coding variants to leprosy susceptibility. But some potential susceptibility loci were not studied in the previous EWAS study because of the project consideration. Seventeen unstudied potential susceptibility loci of the previous EWAS were validated in 3169 cases and 9814 controls in this study. Four disease-associated exonic loci were identified: rs671 in ALDH2 (P = 2.0 × 10-20 , odds ratio [OR] = 1.35), rs13259978 in SLC7A2 (P = 1.74 × 10-8 , OR = 1.28), rs925368 in GIT2 (P = 9.18 × 10-17 , OR = 1.44), and rs75680863 in TCN2 (P = 8.37 × 10-21 , OR = 0.74). Potentially implicating ZFP36L1 as a new susceptibility gene, 1 intergenic single nucleotide polymorphism (SNP), rs1465788 (P = 7.81 × 10-6 , OR = 0.88), was also suggested to be associated with leprosy. A luciferase reporter assay showed that the rs1465788 risk allele notably decreased the transcription activity of the flanking sequence. These findings suggest the possible involvement of lipid metabolism, NF-κB homeostasis and macrophage antimicrobial pathways in leprosy pathogenesis.


Assuntos
Predisposição Genética para Doença , Estudo de Associação Genômica Ampla , Hanseníase/genética , Aldeído-Desidrogenase Mitocondrial/genética , Grupo com Ancestrais do Continente Asiático/genética , Fator 1 de Resposta a Butirato/genética , Transportador 2 de Aminoácidos Catiônicos/genética , DNA Intergênico/genética , Exoma/genética , Éxons/genética , Feminino , Proteínas Ativadoras de GTPase/genética , Humanos , Hanseníase/fisiopatologia , Masculino , NF-kappa B/genética , Polimorfismo de Nucleotídeo Único/genética , Transcobalaminas/genética
5.
Transbound Emerg Dis ; 65(3): 607-613, 2018 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-29521007

RESUMO

PCV3 is an emerging swine virus associated with porcine dermatitis and nephropathy syndrome (PDNS), reproductive failure, respiratory diseases and systematic inflammation. Although first identified in 2015, the earliest case has been traced back to 2009 in the United States. In China, PCV3 infection was first detected in 2015, but little information has been available about its occurrence and prevalence there before 2015. In this study, 200 porcine clinical samples collected from 20 provinces, five autonomous regions and four municipalities between 1990 and 1999 were analysed for PCV3 infection by PCR. Results showed that 6.5% of the porcine samples collected from eight provinces and one autonomous region were PCV3 positive, with the earliest cases occurring in 1996. Nucleotide sequence analysis showed that PCV3 strains obtained in this study shared 96.6%-99.7% and 97.1%-99.4% sequence identity at the ORF2 gene and genome levels with all available reference strains from China and other countries, indicating the high genetic stability of PCV3 over the past 20 years.


Assuntos
Infecções por Circoviridae/epidemiologia , Circovirus/isolamento & purificação , Doenças dos Suínos/epidemiologia , Doenças dos Suínos/virologia , Animais , China/epidemiologia , Infecções por Circoviridae/veterinária , Circovirus/genética , Doenças Transmissíveis Emergentes/veterinária , Doenças Transmissíveis Emergentes/virologia , Filogenia , Prevalência , Estudos Retrospectivos , Suínos
6.
Zhonghua Wai Ke Za Zhi ; 56(3): 183-188, 2018 Mar 01.
Artigo em Chinês | MEDLINE | ID: mdl-29534411

RESUMO

Objective: To explore a standard procedure for the treatment of combined dorsal and palmar internal fixation for complex four part distal radius fractures and assess its clinical results. Methods: From May 2009 to October 2016, 38 patients(39 sides)who suffered from complex four part distal radius fractures were performed operatively with open reduction and internal fixation via combined dorsal and palmar approach in Department of Orthopaedic Trauma, Qilu Hospital of Shandong University(Qingdao). The series included 22 males(22 sides) and 16 females(17 sides). Age of the patients was 53.5 years ranging from 25 to 79 years.According to Melone classification, there were 34 sides of type of Ⅳ, 5 of type Ⅴ.According to Frykman classification, there were 15 sides of type Ⅶ, 24 sides of type Ⅷ, and all the cases were type C3 according to AO/OTA classification.Preoperatively, the key articular fragments in four part distal radius fractures were identified and the individual fracture patterns from conventional X-ray and CT-scan were analyzed. All the patients were performed combined volar and dorsal fixation.Firstly, a palmar approach which gave access to and fix the palmar-ulnar fragment and the radial styloid fragment was performed.Then a limited dorsal approach across the third extensor compartment which gave access to the dorso-ulnar fragment and a limited dorsal arthrotomy to visualize the radiocarpal joint when necessary were performed.Through dorsal approach, we can address the dorso-ulnar fragment, free intra-articular fragment and direct visualize the joint.Use of a retinacular flap was routinely advocated to help prevent against tendon irritation and rupture.The follow-up control included conventional X-ray, range of motion(ROM), grip strength, and the disabilities of the arm, shoulder and hand index(DASH), as well as the patient-rated wrist evaluation(PRWE) score for functional outcome at 6 and 12 months. Results: Thirty-three patients(34 sides) were followed up for at least 12 months.The would healed well in all cases 2 weeks postoperatively, and no soft tissue infections, necrosis or neurovascular complications occurred.All the fractures of 38 cases(39 sides)healed averaged 3.6 months(ranging from 2.5-5.7 months), and no loss of reduction occurred postoperatively.Anatomic reconstruction with a step or gap of <1 mm was achieved in 37 cases(38 sides), Whereas 5 patients were lost to follow-up at 12 months postoperatively.ROM and grip strength were all recovered to over 85% of the unaffected side(exception of the bilateral patient). Median DASH-index and PRWE were 6.5(0-17) and 9.3(0-20)respectively. Conclusion: Combined volar and dorsal approaches allow achieving anatomic reconstruction in complex four part intra-articular distal radius fractures and reveal good functional outcomes at intermediate follow-up.


Assuntos
Fixação Interna de Fraturas , Fraturas do Rádio , Adulto , Idoso , Placas Ósseas , Feminino , Fixação Interna de Fraturas/métodos , Humanos , Masculino , Pessoa de Meia-Idade , Radiografia , Fraturas do Rádio/cirurgia , Amplitude de Movimento Articular , Resultado do Tratamento , Articulação do Punho
7.
J Eur Acad Dermatol Venereol ; 32(6): 1017-1021, 2018 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-29405481

RESUMO

BACKGROUND: The current gold standard for diagnosing onychomycosis is direct microscopic examination and culturing. Fungal culture is a time-consuming procedure, while direct microscopy of potassium hydroxide (KOH) mounts suffers from low sensitivity. More rapid and sensitive methods for the diagnosis of onychomycosis are in high demand. OBJECTIVE: To establish an effective method for the diagnosis of onychomycosis by assessing the efficacies of fungal fluorescent staining and internal transcribed spacer (ITS) ribosomal DNA (rDNA) polymerase chain reaction (PCR)-based sequencing. METHODS: A total of 204 clinical specimens from patients with suspected onychomycosis were analysed. The gold standard for a true positive sample was positive by KOH, culturing or both methods. All specimens were also tested by fungal fluorescent staining and ITS rDNA PCR-based sequencing. We compared the detection, sensitivity and specificity for these two methods with conventional methods. RESULTS: In total, 126 (62%) and 102 (50%) were detected by fluorescent staining and PCR-based sequencing, respectively. According to the conventional diagnostic standard, the sensitivity of fluorescent staining and PCR-based sequencing was 97% and 78%, respectively, and specificities of 89% and 90%, respectively. Use of fluorescence enhanced the sensitivity of direct examination by 12% compared with KOH. PCR-based sequencing increased the sensitivity by 6% compared with culturing. CONCLUSIONS: Fluorescence microscopy has a higher sensitivity for the detection of fungi in nail specimens compared with KOH and can be used as a rapid screening tool. PCR-based sequencing was faster and more sensitive compared with culture and when used in conjunction with fluorescence microscopy resulted in higher efficiency.


Assuntos
DNA Fúngico/genética , DNA Ribossômico/genética , Corantes Fluorescentes/química , Onicomicose/diagnóstico , Reação em Cadeia da Polimerase/métodos , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Criança , Pré-Escolar , Feminino , Fungos/classificação , Fungos/genética , Humanos , Masculino , Microscopia de Fluorescência , Pessoa de Meia-Idade , Unhas/microbiologia , Onicomicose/microbiologia , Valor Preditivo dos Testes , Sensibilidade e Especificidade , Adulto Jovem
8.
Transbound Emerg Dis ; 65(2): 432-440, 2018 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-28963755

RESUMO

Porcine postweaning multisystemic wasting syndrome (PMWS) caused by porcine circovirus type 2 (PCV2) is a disease causing severe economic losses annually worldwide to the pig industry. PCV2 infection was first reported in China in 2000, and currently has three major genotypes, PCV2a, b and d, circulating in this country. To further elucidate the origin and prevalence of PCV2 in China, 123 clinical pig tissue samples collected in 25 provinces between 1990 and 1999 were analysed by PCV2-specific PCR, resulting in identification of 23 PCV2 strains collected between 1996 and 1999. Phylogenetic analysis based on the nucleotide sequences of open reading frame 2 (ORF2) showed that 20 of the 23 grouped within PCV2a, while the remaining three strains formed an independent clade, so far unreported and therefore named PCV2f. This genotype shared lower sequence identity with other known genotypes. This study provides further understanding of the genetic diversity and evolution of PCV2 and has tracked PCV2 infection in China back to 1996 rather than 2000.


Assuntos
Circovirus/genética , Variação Genética , Genoma Viral/genética , Síndrome Definhante Multissistêmico de Suínos Desmamados/virologia , Doenças dos Suínos/virologia , Animais , China/epidemiologia , Circovirus/isolamento & purificação , DNA Viral/genética , Genótipo , Fases de Leitura Aberta , Filogenia , Reação em Cadeia da Polimerase/veterinária , Síndrome Definhante Multissistêmico de Suínos Desmamados/epidemiologia , Prevalência , Estudos Retrospectivos , Suínos , Doenças dos Suínos/epidemiologia
9.
Zhonghua Bing Li Xue Za Zhi ; 46(9): 648-649, 2017 Sep 08.
Artigo em Chinês | MEDLINE | ID: mdl-28910880
10.
Exp Neurol ; 295: 125-134, 2017 09.
Artigo em Inglês | MEDLINE | ID: mdl-28587875

RESUMO

We have previously reported that administration of a CD11d monoclonal antibody (mAb) improves recovery in a clip-compression model of SCI. In this model the CD11d mAb reduces the infiltration of activated leukocytes into the injured spinal cord (as indicated by reduced intraspinal MPO). However not all anti-inflammatory strategies have reported beneficial results, suggesting that success of the CD11d mAb treatment may depend on the type or severity of the injury. We therefore tested the CD11d mAb treatment in a rat hemi-contusion model of cervical SCI. In contrast to its effects in the clip-compression model, the CD11d mAb treatment did not improve forelimb function nor did it significantly reduce MPO levels in the hemi-contused cord. To determine if the disparate results using the CD11d mAb were due to the biomechanical nature of the cord injury (compression SCI versus contusion SCI) or to the spinal level of the injury (12th thoracic level versus cervical) we further evaluated the CD11d mAb treatment after a T12 contusion SCI. In contrast to the T12 clip compression SCI, the CD11d mAb treatment did not improve locomotor recovery or significantly reduce MPO levels after T12 contusion SCI. Lesion analyses revealed increased levels of hemorrhage after contusion SCI compared to clip-compression SCI. SCI that is accompanied by increased intraspinal hemorrhage would be predicted to be refractory to the CD11d mAb therapy as this approach targets leukocyte diapedesis through the intact vasculature. These results suggest that the disparate results of the anti-CD11d treatment in contusion and clip-compression models of SCI are due to the different pathophysiological mechanisms that dominate these two types of spinal cord injuries.


Assuntos
Antígenos CD11/efeitos dos fármacos , Hemorragia/complicações , Traumatismos da Medula Espinal/tratamento farmacológico , Animais , Anticorpos Bloqueadores/farmacologia , Anticorpos Monoclonais/farmacologia , Vértebras Cervicais/lesões , Membro Anterior , Locomoção , Masculino , Peroxidase/metabolismo , Ratos , Ratos Sprague-Dawley , Ratos Wistar , Recuperação de Função Fisiológica , Compressão da Medula Espinal/tratamento farmacológico , Compressão da Medula Espinal/fisiopatologia , Traumatismos da Medula Espinal/complicações , Vértebras Torácicas/lesões , Migração Transendotelial e Transepitelial/efeitos dos fármacos
11.
Front Microbiol ; 7: 1867, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27965627

RESUMO

Phage-mediated horizontal gene transfer (HGT) is common in free-living bacteria, and many transferred genes can play a significant role in their new bacterial hosts. However, there are few reports concerning phage-mediated HGT in endosymbionts (obligate intracellular bacteria within animal or plant hosts), such as Wolbachia. The Wolbachia-infecting temperate phage WO can actively shift among Wolbachia genomes and has the potential to mediate HGT between Wolbachia strains. In the present study, we extend previous findings by validating that the phage WO can mediate transfer of non-phage genes. To do so, we utilized bioinformatic, phylogenetic, and molecular analyses based on all sequenced Wolbachia and phage WO genomes. Our results show that the phage WO can mediate HGT between Wolbachia strains, regardless of whether the transferred genes originate from Wolbachia or other unrelated bacteria.

12.
Zhonghua Yan Ke Za Zhi ; 52(9): 674-80, 2016 Sep 11.
Artigo em Chinês | MEDLINE | ID: mdl-27647248

RESUMO

OBJECTIVE: To establish a finite-element biomechanical model of astigmatic keratotomy, and to investigate the impact of surgical parameters on corneal deformation, stress distribution and astigmatism correction. METHODS: With Rhinoceros modeling and Abaqus finite element analysis software, a three-dimensional finite-element model of astigmatic cornea was developed, and surgical parameters such as incision optical zone, incision depth and length were varied. Postoperative corneal stress, apical deformation and astigmatism correction were assessed. RESULTS: A significant increase of stress was noticed near corneal incisions, and maximum corneal stress decreased with the increase of incision depth. Both anterior and posterior corneal surface moved slightly forward postoperatively. Maximum corneal stress was 340 392, 361 022 and 214 187 Pa, and anterior and posterior apical deformation was 49.80, 51.64, 55.53 µm and 54.15, 55.91, 59.67 µm, with 45°, 60° and 90° in arc length of the incision, respectively. The refractive power decreased in steep meridian and increased in flat meridian, resulting in a total decrease of corneal astigmatism. The magnitude of astigmatism correction was 0.85, 1.59, 2.23 and 3.06 D with 30°, 45°, 60° and 90° in arc length of the incision, respectively. CONCLUSIONS: The finite-element biomechanical model of astigmatic keratotomy could be used to predict the optical outcomes after surgery. The magnitude of astigmatism correction is positively correlated with the surgical incision arc length. (Chin J Ophthalmol, 2016, 52: 674-680).


Assuntos
Astigmatismo/cirurgia , Córnea/fisiologia , Ceratotomia Radial , Astigmatismo/fisiopatologia , Extração de Catarata , Córnea/cirurgia , Topografia da Córnea , Análise de Elementos Finitos , Humanos , Refração Ocular/fisiologia
13.
Colorectal Dis ; 18(9): O322-9, 2016 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-27438774

RESUMO

AIM: The aim of this study was to evaluate the prognostic impact of lymph node skip metastasis (LNSM) in patients with Stage III colorectal cancer. METHOD: Between April 2003 and December 2014, a total of 41 patients with lymph node skip metastasis (skip+) were compared with 86 patients with pericolic lymph node metastases [lymph node distribution (LND)1] and 57 patients with intermediate and/or main lymph node metastasis (LND2+3). All patients had radical D3 lymphadenectomy, performed either laparoscopically or as open surgery. RESULTS: The frequency of pT1-2 stage cancer was significantly higher in the skip+ group than in the LND1 group (26.8% vs 5.8%, P = 0.001). The number of metastatic lymph nodes in the skip+ group was lower than in the LND2+3 group (1.9 ± 1.5 vs 6.5 ± 6.0, P < 0.001). The 3-year disease-free survival (DFS) of the skip+, LND1 and LND2+3 groups was 64.8%, 69.7% and 40.1%, respectively (P = 0.008). The 3-year systemic recurrence rates of the skip+, LND1 and LND2+3 groups were 30.2%, 20.3% and 48.1%, respectively; (P = 0.002). Cox regression analysis revealed that preoperative carcinoembryonic antigen (CEA) of ≥ 5 ng/ml [hazard ratio (HR) = 2.2, P = 0.029], poor differentiation (HR = 3.8, P = 0.001) and skip+ (HR = 0.2, P = 0.021) were independently prognostic factors for DFS. CONCLUSION: The prognosis for the LND1-negative lymph node skip metastasis group was better than for the LND2+3 group and was comparable with that of the LND1 group after radical D3 lymphadenectomy.


Assuntos
Adenocarcinoma/cirurgia , Neoplasias Colorretais/cirurgia , Procedimentos Cirúrgicos do Sistema Digestório/métodos , Excisão de Linfonodo/métodos , Linfonodos/patologia , Recidiva Local de Neoplasia/epidemiologia , Adenocarcinoma/sangue , Adenocarcinoma/patologia , Adulto , Idoso , Antígeno Carcinoembrionário/sangue , Estudos de Coortes , Neoplasias Colorretais/sangue , Neoplasias Colorretais/patologia , Intervalo Livre de Doença , Feminino , Humanos , Laparoscopia , Metástase Linfática/patologia , Masculino , Pessoa de Meia-Idade , Análise Multivariada , Estadiamento de Neoplasias , Prognóstico , Modelos de Riscos Proporcionais , Estudos Retrospectivos
14.
Nanoscale ; 8(23): 11956-61, 2016 Jun 09.
Artigo em Inglês | MEDLINE | ID: mdl-27240477

RESUMO

A novel C3N4-CDot composite photocatalyst was very recently shown to be highly efficient and very stable in water splitting by solar radiation without using any sacrificial reagent (J. Liu, et al., Science, 2015, 347(6225), 970). This photocatalyst utilizes a two-electron/two-step process in which the production of H2O2 and H2 is photocatalyzed by using C3N4 in the first step and H2O2 is decomposed by using CDots in the second step. The present work is a study on the generality of this approach by application of a C3N4/MnO2 catalyst. This new catalyst indeed splits water by a two step process in a stable way, without any sacrificial agent. It was however found that though the absorbance of the new catalyst in the visible range of 500-600 nm is much larger than that of the C3N4-CDot catalyst, its water splitting efficiency is much lower. These findings add insight into and assist in the further optimization of this new class of photocatalysts to meet the requirements of commercial water splitting systems.

15.
Br J Dermatol ; 174(1): 176-9, 2016 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-26440558

RESUMO

Corynespora cassiicola is a plant pathogen associated with leaf-spotting disease. The fungus has been found on diverse substrates: leaves, stems and roots of plants; nematode cysts and human skin. It rarely causes human infections. Here we report one case of subcutaneous phaeohyphomycosis caused by C. cassiicola with prominent tissue necrosis in a woman. All of her clinical features pointed towards a genetic linkage. Hence, whole-exome sequencing and Sanger sequencing were performed on this patient. One mutation of CARD9 was detected.


Assuntos
Ascomicetos , Proteínas Adaptadoras de Sinalização CARD/genética , Dermatomicoses/genética , Dermatoses Faciais/genética , Mutação/genética , Adulto , Proteínas Adaptadoras de Sinalização CARD/deficiência , Feminino , Humanos
16.
Neuroscience ; 289: 56-62, 2015 Mar 19.
Artigo em Inglês | MEDLINE | ID: mdl-25595976

RESUMO

Hemifacial spasm (HFS) is a peripheral nerve disorder which impacts the living quality of patients both psychologically and physically. Whether HFS has structural changes under these specific stressors including psychological and physiological conditions in the CNS remains largely unknown. In the current study, voxel-based morphometry (VBM) was used to evaluate changes in gray matter (GM) by using T1-weighted imaging in 25 HFS patients and 25 demographically similar healthy volunteers. The severity of the spasm was assessed using a Cohen evaluation scale. Hamilton anxiety (HAMA) and Hamilton depression (HAMD) rating scales were used to evaluate the affective conditions of subjects. 3D-FIESTA and 3D-TOF sequences were applied to evaluate the neurovascular compression (NVC) rating in each subject. In our results, we found that HFS patients had higher NVC rating scores than those of healthy volunteers, and the spasm severity rating was positively correlated with the NVC rating (r=0.736, p<0.001). HFS patients had higher scores on the HAMA and HAMD compared with healthy volunteers. For the GM comparison, reductions were found in the thalamus, putamen, pallidum, dorsolateral prefrontal cortex, amygdala and parahippocampal gyrus in patients with HFS compared with healthy volunteers. Additionally, the GM volume changes in the amygdala did not exhibit any significant between-group differences with HAMA and HAMD scores as covariates. Our results suggested that HFS probably led to GM volume abnormalities of the CNS. We indicated that the GM volume changes of the amygdala may be highly related to emotional factors.


Assuntos
Encéfalo/patologia , Substância Cinzenta/patologia , Espasmo Hemifacial/patologia , Ansiedade/patologia , Depressão/patologia , Feminino , Espasmo Hemifacial/psicologia , Humanos , Processamento de Imagem Assistida por Computador , Imagem por Ressonância Magnética , Masculino , Pessoa de Meia-Idade , Tamanho do Órgão , Escalas de Graduação Psiquiátrica , Índice de Gravidade de Doença , Inquéritos e Questionários
17.
Genetika ; 51(10): 1212-6, 2015 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-27169238

RESUMO

The isolation and development of 15 polymorphic dinucleotide microsatellite loci were described for Ophicephalus argus from the Huaihe River. All loci were polymorphic in the 30 individuals tested. The number of alleles per variable locus ranged from nine to seventeen, with a mean of 12.00. These novel microsatellite loci showed high level of polymorphism. Observed and expected heterozygosities ranged from 0.793 to 0.929 and from 0.841 to 0.952, respectively. Two loci were found deviated from HWE in the sampled population after Bonferroni correction. These microsatellite loci will be useful for revealing population structure, genetic diversity, and phylogeography of Ophicephalus argus.


Assuntos
Repetições de Dinucleotídeos , Peixes/genética , Loci Gênicos , Polimorfismo Genético , Animais
18.
Neuroscience ; 285: 81-96, 2015 Jan 29.
Artigo em Inglês | MEDLINE | ID: mdl-25451281

RESUMO

We previously demonstrated that hydrogen peroxide concentration ([H2O2]) significantly increases after spinal cord injury (SCI). The present study explored (1) whether SCI-elevated [H2O2] is sufficient to induce oxidation and cell death, (2) if apoptosis is a pathway of H2O2-induced cell death, and (3) whether H2O2-induced oxidation and cell death could be reversed by treatment with the catalytic antioxidant Mn (III) tetrakis (4-benzoic acid) porphyrin (MnTBAP). H2O2 was perfused through a microcannula into the uninjured rat spinal cord to mimic the conditions induced by SCI. Protein and DNA oxidation, membrane phospholipids peroxidation (MLP), cell death and apoptosis were characterized by histochemical and immunohistochemical staining with antibodies against markers of oxidation and apoptosis. Stained cells were quantified in sections of H2O2-, or artificial cerebrospinal fluid (ACSF)-exposed with vehicle-, or MnTBAP-treated groups. Compared with ACSF-exposed animals, SCI-elevated [H2O2] significantly increased intracellular protein and DNA oxidation by threefold and MLP by eightfold in neurons, respectively. H2O2-elevated extracellular malondialdehyde was measured by microdialysis sampling. We demonstrated that SCI-elevated [H2O2] significantly increased extracellular malondialdehyde above pre-injury levels. H2O2 also significantly increased cell loss and the numbers of terminal deoxynucleotidyl transferase (TdT)-mediated deoxyuridine triphosphate-(dUTP)-biotin nick end labeling (TUNEL)-positive and active caspase-3-positive neurons by 2.3-, 2.8-, and 5.6-fold compared to ACSF controls, respectively. Our results directly and unequivocally demonstrate that SCI-elevated [H2O2] contributes to post-SCI MLP, protein, and DNA oxidation to induce cell death. Therefore, we conclude that (1) the role of H2O2 in secondary SCI is pro-oxidation and pro-cell death, (2) apoptosis is a pathway for SCI-elevated [H2O2] to induce cell death, (3) caspase activation is a mechanism of H2O2-induced apoptosis after SCI, and (4) MnTBAP treatment significantly decreased H2O2-induced oxidation, cell loss, and apoptosis to the levels of ACSF controls, further supporting MnTBAP's ability to scavenge H2O2 by in vivo evidence.


Assuntos
Morte Celular/fisiologia , Peróxido de Hidrogênio/metabolismo , Traumatismos da Medula Espinal/fisiopatologia , Medula Espinal/fisiopatologia , Animais , Antioxidantes/farmacologia , Caspases/metabolismo , Morte Celular/efeitos dos fármacos , DNA/efeitos dos fármacos , DNA/metabolismo , Modelos Animais de Doenças , Espaço Extracelular/metabolismo , Peróxido de Hidrogênio/administração & dosagem , Masculino , Metaloporfirinas/farmacologia , Neurônios/efeitos dos fármacos , Neurônios/patologia , Neurônios/fisiologia , Oxirredução , Fosfolipídeos/metabolismo , Proteínas/metabolismo , Ratos Sprague-Dawley , Medula Espinal/efeitos dos fármacos , Medula Espinal/patologia , Traumatismos da Medula Espinal/tratamento farmacológico , Traumatismos da Medula Espinal/patologia
19.
Eur Rev Med Pharmacol Sci ; 18(20): 3038-44, 2014 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-25392102

RESUMO

OBJECTIVE: Osteosarcoma (OS) is the most common primary malignant bone tumour in children and adolescents. Despite aggressive therapy, survival outcomes remain unsatisfactory, especially for patients with metastatic disease or patients with a poor chemotherapy response. Previous study founds inosine 5'-monophosphate dehydrogenase type II (IMPDH2) was an independent prognostic factor and observed frequent IMPDH2 overexpression in osteosarcoma patients with poor response to chemotherapy. In the present work, we provide evidence for direct involvement of IMPDH2 in the development of radioresistance and chemoresistance. MATERIALS AND METHODS: The expression of IMPDH2 was examined in OS cells. Stable cell lines overexpressing IMPDH2 and IMPDH2 knock-down cells were generated using the osteosarcoma cell line. The stable transfected cells, alone or in combination with cisplatin or γ-irradiation, was used to treat OS cells. The growth inhibitory and apoptotic effects of IMPDH2 in vitro and in vivo were examined. RESULTS: Overexpression of IMPDH2 in IMPDH2 poor-expressed U2OS cells induced strong cisplatin chemoresistance and γ-irradiation radioresistance through inhibition of apoptosis in vitro and in vivo. Knockdown of IMPDH2 in IMPDH2 rich-expressed Saos-2 cells resulted in significant chemosensitivity and γ-irradiation radiosensitivity through inducing of apoptosis in vitro and in vivo. CONCLUSIONS: IMPDH2 is directly involved in the development of chemoresistance and radioresistance in osteosarcoma cells, suggesting that targeting of IMPDH2 by shRNA in combination with chemotherapy and γ-irradiation might be a promising means of overcoming chemoresistance and radioresistance in osteosarcomas with high IMPDH2 expression.


Assuntos
Neoplasias Ósseas/tratamento farmacológico , Neoplasias Ósseas/radioterapia , IMP Desidrogenase/fisiologia , Osteossarcoma/tratamento farmacológico , Osteossarcoma/radioterapia , Animais , Apoptose/efeitos dos fármacos , Apoptose/fisiologia , Neoplasias Ósseas/metabolismo , Linhagem Celular Tumoral , Cisplatino/farmacologia , Cisplatino/uso terapêutico , Resistencia a Medicamentos Antineoplásicos/fisiologia , Feminino , Humanos , Camundongos Nus , Osteossarcoma/metabolismo
20.
Acta Anaesthesiol Scand ; 58(7): 858-66, 2014 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-24961586

RESUMO

BACKGROUND: We undertook a prospective study of non-obstetric epidurals placed in surgical inpatients at a single teaching hospital to evaluate the incidence of and potential risk factors for major complications of continuous epidural anesthesia. METHODS: Demographic information, details of the epidural procedure, and complications (from the pre-anesthetic period through resolution) were recorded for more than 5000 surgical inpatients who underwent continuous epidural anesthesia in our institution between March 2009 and April 2011. The incidence of and risk factors for major complications were evaluated. RESULTS: During the study period, 5083 patients were interviewed and their details were recorded (98% capture rate). Sixty-nine (1.36%) experienced major complications: epidural hematoma in 1 patient (0.02%), post-operative neurologic deficits in 57 patients (1.12%), post-dural puncture headache in 7 patients (0.14%), and systemic local anesthetic toxicity in 4 patients (0.08%). Only one patient had permanent sequelae: unilateral lower limb paresthesia. Identified risk factors for neurologic deficits were as follows: American Society of Anesthesiologists status II-III, siting in the lumbar region, orthopedic and urologic surgery, multiple attempts to site an epidural, paresthesia during insertion, a history of neuraxial anesthesia, and use of patient-controlled epidural analgesia. CONCLUSIONS: Serious complications were very rare; only one patient had permanent sequelae, and a single epidural hematoma was diagnosed. Post-operative neurologic deficits were more common, but most complications resolved spontaneously within 3 months and they rarely required intervention.


Assuntos
Anestesia Epidural/efeitos adversos , Adulto , Idoso , Analgesia Controlada pelo Paciente , Anestésicos Locais/administração & dosagem , Anestésicos Locais/efeitos adversos , Dura-Máter/lesões , Feminino , Hematoma/epidemiologia , Hematoma/etiologia , Humanos , Hipestesia/epidemiologia , Hipestesia/etiologia , Incidência , Perna (Membro)/inervação , Masculino , Pessoa de Meia-Idade , Neuralgia/epidemiologia , Neuralgia/etiologia , Parestesia/epidemiologia , Parestesia/etiologia , Cefaleia Pós-Punção Dural/epidemiologia , Cefaleia Pós-Punção Dural/etiologia , Estudos Prospectivos , Fatores de Risco
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