Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 9 de 9
Filtrar
Mais filtros










Intervalo de ano de publicação
1.
Braz. j. biol ; 82: e233523, 2022. tab
Artigo em Inglês | LILACS-Express | LILACS | ID: biblio-1153470

RESUMO

Abstract Microbiological studies of the sanitary and health status of psittacine birds that will be reintroduced is important in evaluating whether these animals act as carriers of pathogenic agents to other animals and humans. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a faster and more accurate method to identify bacteria than conventional microbiology methods. The aim of this study was to evaluate the health status of psittacines housed in captivity, by assessment of Gram-negative bacteria from fecal microbiota through MALDI- TOF MS identification. The results indicate high frequency of Gram-negative bacteria in feces (96.5%), especially from the Enterobacteriaceae family (88.7%). The most prevalent bacteria were Escherichia coli (39.0%), Proteus vulgaris (12.2%), Klebsiella spp. (12.1%) and Raoultella ornithinolytica (8.7%). Proteus hauseri, Citrobacter spp., Morganella morgannii, Providencia rettgeri, Enterobacter spp. and Escherichia hermannii were isolated with lower frequency. . All these agents are potentially pathogenic for parrots and can cause systemic infections in other animals and humans. These findings reinforce that MALDI- TOF MS proved to be a rapid and accurate method of identification of the microorganism and evaluation of the health status of psittacines, providing relevant data to assist decision-making regarding the sanitary protocols in wildlife centers, and possible future reintroduction of wild birds.


Resumo Estudos microbiológicos da sanidade de psitacídeos que serão reintroduzidos são importantes para avaliar se esses animais atuam como portadores de agentes patogênicos para outros animais e humanos. A espectrometria de massa por ionização/dessorção de matriz assistida por laser/tempo de vôo (MALDI-TOF MS) é um método mais rápido e preciso para identificar bactérias na comparação com métodos convencionais de microbiologia. O objetivo deste estudo foi avaliar o estado de saúde de psitacídeos cativos, identificando bactérias Gram-negativas da microbiota fecal por MALDI -TOF MS. Os resultados indicaram alta frequência de bactérias Gram-negativas nas fezes (96,5%), principalmente da família Enterobacteriaceae (88,7%). As mais prevalentes foram Escherichia coli (39,0%), Proteus vulgaris (12,2%), Klebsiella spp. (12,1%) e Raoultella ornithinolytica (8,7%). Proteus hauseri, Citrobacter spp., Morganella morgannii, Providencia rettgeri, Enterobacter spp. e Escherichia hermannii foram isolados com menor frequência. Todos esses agentes são potencialmente patogênicos para os papagaios e podem causar infecções sistêmicas em outros animais e seres humanos. Esses achados reforçam que o MALDI- TOF MS é um método rápido e preciso de identificação do microrganismo e avaliação do estado de saúde dos psitacídeos, fornecendo dados relevantes para auxiliar na tomada de decisões sobre os protocolos sanitários em centros de triagem de animais selvagens e sobre a possibilidade de reintrodução futura.

2.
Braz J Biol ; 82: e233523, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33787713

RESUMO

Microbiological studies of the sanitary and health status of psittacine birds that will be reintroduced is important in evaluating whether these animals act as carriers of pathogenic agents to other animals and humans. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a faster and more accurate method to identify bacteria than conventional microbiology methods. The aim of this study was to evaluate the health status of psittacines housed in captivity, by assessment of Gram-negative bacteria from fecal microbiota through MALDI- TOF MS identification. The results indicate high frequency of Gram-negative bacteria in feces (96.5%), especially from the Enterobacteriaceae family (88.7%). The most prevalent bacteria were Escherichia coli (39.0%), Proteus vulgaris (12.2%), Klebsiella spp. (12.1%) and Raoultella ornithinolytica (8.7%). Proteus hauseri, Citrobacter spp., Morganella morgannii, Providencia rettgeri, Enterobacter spp. and Escherichia hermannii were isolated with lower frequency. . All these agents are potentially pathogenic for parrots and can cause systemic infections in other animals and humans. These findings reinforce that MALDI- TOF MS proved to be a rapid and accurate method of identification of the microorganism and evaluation of the health status of psittacines, providing relevant data to assist decision-making regarding the sanitary protocols in wildlife centers, and possible future reintroduction of wild birds.


Assuntos
Bactérias Gram-Negativas , Psittaciformes , Animais , Enterobacteriaceae , Humanos , Proteus , Providencia , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
3.
Arq. bras. med. vet. zootec ; 66(3): 797-800, 06/2014. graf
Artigo em Inglês | LILACS | ID: lil-718061

RESUMO

A seven-year-old female spayed Schnauzer was presented with cutaneous ulcerated nodular lesions shortly after the beginning of an immunosuppressive treatment for immune-mediated hemolytic disease. Cytology was performed and a great number of neutrophils and banana-shaped organisms were observed. Biopsy showed a neutrophilic and histiocytic dermatitis and panniculitis with myriads of intralesional bradyzoites cysts and tachyzoites. PCR analysis was positive for Toxoplasma gondii and negative for Neospora caninum. Immunohistochemistry confirmed intralesional T. gondii antigens. This study reports a rare case of cutaneous toxoplasmosis in an immunosuppressed dog...


Uma cadela Schnauzer, castrada, de sete anos apresentou lesões cutâneas nodulares ulceradas pouco tempo após início de tratamento imunossupressor para doença hemolítica imunomediada. Foi realizado exame citológico, e um grande número de neutrófilos e estruturas em forma de banana foi observado. Biópsia mostrou dermatite e paniculite neutrofílica e histiocítica com miríades de taquizoítos e cistos de bradizoítos intralesionais. PCR foi positivo para Toxoplasma gondii e negativo para Neospora caninum. Imuno-histoquímica confirmou antígenos de T. gondii intralesionais. Este trabalho relata um caso raro de toxoplasmose cutânea em um cão imunossuprimido...


Assuntos
Animais , Feminino , Cães , Cães/parasitologia , Dermatite/veterinária , Hospedeiro Imunocomprometido , Paniculite/veterinária , Toxoplasmose Animal , Toxoplasma/isolamento & purificação , Icterícia/veterinária , Reação em Cadeia da Polimerase/veterinária
4.
Lupus ; 20(3): 265-73, 2011 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-21233146

RESUMO

Systemic lupus erythematosus (SLE) is an autoimmune disorder of the connective tissue with a wide and heterogeneous spectrum of manifestations, with renal and neurological involvement usually related to worse prognosis. SLE more frequently affects females of reproductive age, and a high prevalence and renal manifestation seem to be associated with non-European ethnicity. The present study aims to investigate candidate loci to SLE predisposition and evaluate the influence of ethnic ancestry in the disease risk and clinical phenotypic heterogeneity of lupus at onset. Samples represented by 111 patients and 345 controls, originated from the city of Belém, located in the Northern Region of Brazil, were investigated for polymorphisms in HLA-G, HLA-C, SLC11A1, MTHFR, CASP8 and 15 KIR genes, in addition to 89 Amerindian samples genotyped for SLC11A1. We also investigated 48 insertion/deletion ancestry markers to characterize individual African, European and Amerindian ancestry proportions in the samples. Predisposition to SLE was associated with GTGT deletion at the SLC11A1 3'UTR, presence of KIR2DS2 +/KIR2DS5 +/KIR3DS1 + profile, increased number of stimulatory KIR genes, and European and Amerindian ancestries. The ancestry analysis ruled out ethnic differences between controls and patients as the source of the observed associations. Moreover, the African ancestry was associated with renal manifestations.


Assuntos
Proteínas de Transporte de Cátions/genética , Grupos Étnicos/genética , Marcadores Genéticos , Predisposição Genética para Doença , Lúpus Eritematoso Sistêmico/genética , Polimorfismo Genético , Receptores KIR/genética , Adulto , Idade de Início , Brasil , Cidades , Feminino , Frequência do Gene , Humanos , Lúpus Eritematoso Sistêmico/etnologia , Masculino , Receptores KIR3DS1/genética
5.
J Neuroendocrinol ; 23(2): 148-57, 2011 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-21091554

RESUMO

Protein restriction during lactation has been suggested to diminish parasympathetic activity, whereas sympathetic activity is enhanced in adult rats. The present study analyses whether dysfunction of the autonomic nervous system is involved in the impairment of insulin secretion from perinatally undernourished rats. Male neonates were reared by mothers fed a low- (4%) protein (LP group) or normal- (23%) protein diet (NP group). At 81 days of age, LP rats showed less body mass than NP rats (318 ± 4 g versus 370 ± 5 g) (P < 0.001). Fat tissue accumulation decreased in LP [0.8 ± 0.03 g/100 g body weight (BW)] compared to NP rats (1.1 ± 0.04 g/100 g BW) (P < 0.001). LP were glucose-intolerant as registered by the area under the curve of an i.v. glucose tolerance test (37 ± 3) compared to NP rats (29 ± 2) (P < 0.05); however, LP animals showed fasting normoglycaemia (LP, 5.0 ± 0.1; NP, 4.9 ± 0.03 mm) and hypoinsulinaemia (LP, 0.10 ± 0.02 ng/ml; NP, 0.17 ± 0.02 ng/ml). LP also showed glucose tissue uptake 60% higher than NP rats (P < 0.05). Vagus firing rate from LP was lower (7.1 ± 0.8 spikes/5 s) than that in NP rats (12.3 ± 0.7 spikes/5 s) (P < 0.001); however, there was no difference in sympathetic nervous activity. The cholinergic insulinotrophic effect was lower in pancreatic islets from LP (0.07 ± 0.01 ng/min/islet) than in NP rats (0.3 ± 0.06 ng/min/islet), whereas the levels of adrenaline-mediated inhibition of glucose-induced insulin release were similar. Perinatal protein restriction inhibited the activity of the vagus nerve, thus reducing the insulinotrophic effect of parasympathetic pathways on pancreatic ß-cells, which inhibit insulin secretion.


Assuntos
Glucose/metabolismo , Insulina/metabolismo , Desnutrição Proteico-Calórica/fisiopatologia , Nervo Vago/fisiologia , Medula Suprarrenal/metabolismo , Animais , Animais Recém-Nascidos , Catecolaminas/metabolismo , Dieta com Restrição de Proteínas , Feminino , Glucose/farmacologia , Teste de Tolerância a Glucose , Células Secretoras de Insulina/efeitos dos fármacos , Células Secretoras de Insulina/metabolismo , Lactação/fisiologia , Masculino , Ratos
6.
Res Commun Mol Pathol Pharmacol ; 106(1-2): 63-76, 1999.
Artigo em Inglês | MEDLINE | ID: mdl-11127809

RESUMO

The effects of diets with different protein levels were evaluated when given at different intervals to lactating rats. Food intake by the litter and weight gain by the young are investigated. From the time of birth, groups of five litters were fed with commercial diet (23% protein) or with semi-synthetic diets (4,8,12 and 16% protein) during the 1st, 1st and 2nd, or 1st, 2nd and 3rd weeks of lactation. After weaning, the young of dams, which were fed hypoprotein diets (4 and 8%), showed less food intake capacity, reduced growth capacity and, in adult life, low body weight in comparison to animals raised on commercial ration with higher protein contents. 12 and 16% protein diets did not cause any change in feeding behavior or in weight development as opposed to the 4 and 8% protein diets. Results suggest that the effects of early undernourishment are time-dependent and may cause irreversible changes in the regulation of metabolism and pathogenesis.


Assuntos
Lactação , Deficiência de Proteína/fisiopatologia , Animais , Animais Recém-Nascidos , Regulação do Apetite , Feminino , Masculino , Ratos , Ratos Wistar , Fatores de Tempo , Ganho de Peso
7.
Res Commun Mol Pathol Pharmacol ; 92(1): 73-84, 1996 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-8733829

RESUMO

In order to characterize an alternative animal model for the study of diabetes mellitus type II onset, we compared the effects of a diet containing 8% of protein (LPD) and a normal diet containing 25% of protein supplied to the dams during the first 12 days of lactation. We studied in the pups the growth evolution and, when they develop into adults (60 days), the glucose tolerance test (GTT) and the insulin secretion, in response to stimulatory concentrations of glucose. The weight of the two groups were significantly different at 60 days of age (LPD = 179 +/- 19 g; NPD = 186 +/- 18 g). The GTT ten minutes after iv glucose administration showed a significant increase of blood glucose concentration of the LPD group (LPD = 550 +/- 17 mg/dl; NPD = 425 +/- 13 mg/dl, p < 0.001). The insulin secretion, four minutes after stimulation was found reduced in the LPD group (LPD = 1.1 +/- 0.08 muU/islet/min; NPD = 1.85 +/- 0.2 muU/islet/min.). The present study indicates insulin secretory and/or resistance impairment due to early undernutrition. Also, the data taken together suggest that undernutrition during early lactation can be used as an alternative model to study particular characteristics of the onset of diabetes mellitus type II.


Assuntos
Diabetes Mellitus Experimental/metabolismo , Lactação/metabolismo , Animais , Glicemia/metabolismo , Modelos Animais de Doenças , Feminino , Insulina/metabolismo , Gravidez , Ratos , Fatores de Tempo
8.
Braz J Med Biol Res ; 26(12): 1355-8, 1993 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-8136737

RESUMO

In order to study the effect of nutrition on the onset of disturbances in Wistar rat pancreatic beta cells, we compared the effects of a low protein diet (8% protein) and a normal protein diet (25% protein) supplied to the dams (6 in each group) during the first 12 days of lactation. The parameter evaluated was the beta cells phosphate flush in response to stimulatory concentration of glucose (16.7 mM) of isolated islets of Langerhans from 60-day old pups. Using a collagenase digestion technique, islets were isolated from the pups and the 32P fractional outflow rate (FOR) of the beta cells was used as a metabolic index in both experimental groups (N = 36). We observed that although the weights of the pups of the two groups were not significantly different at 60 days of age (control = 186 +/- 18 g; undernourished during lactation = 179 +/- 19 g), the typical phosphate flush response (FOR = 2.4 +/- 0.4%/min) to a stimulatory glucose concentration (16.7 mM) was abolished in the rats from undernourished mothers. Our data are consistent with the hypothesis that undernutrition may be an important cause of diabetes mellitus type II.


Assuntos
Proteínas na Dieta/administração & dosagem , Ilhotas Pancreáticas/metabolismo , Lactação , Fosfatos/metabolismo , Radioisótopos de Fósforo/metabolismo , Animais , Animais Recém-Nascidos , Animais Lactentes , Peso Corporal , Feminino , Glucose/administração & dosagem , Gravidez , Ratos , Ratos Wistar , Fatores de Tempo
9.
Braz. j. med. biol. res ; 26(12): 1355-8, Dec. 1993. graf
Artigo em Inglês | LILACS | ID: lil-148841

RESUMO

In order to study the effect of nutrition on the onset of disturbances in Wistar rat pancreatic beta cells, we compared the effects of a low protein diet (8 per cent protein) and a normal protein diet (25 per cent protein) supplied to the dams (6 in each group) during the first 12 days of lactation. The parameter evaluated was the beta cells phosphate flush in response to stimulatory concentration of glucose (16.7 mM) of isolated islets of Langerhans from 60-day old pups. Using a collagenase digestion technique, islets were isolated from the pups and the 32P fractional outflow rate (FOR) of the beta cells was used as a metabolic index in both experimental groups (N = 36). We observed that although the weights of the pups of the two groups were not significantly different at 60 days of age (control = 186 +/- 18 g; undernourished during lactation = 179 +/- 19 g), the typical phosphate flush response (FOR = 2.4 +/- 0.4 per cent /min) to a stimulatory glucose concentration (16.7 mM) was abolished in the rats from undernourished mothers. Our data are consistent with the hypothesis that undernutrition may be an important cause of diabetes mellitus type II


Assuntos
Animais , Feminino , Gravidez , Ratos , Ilhotas Pancreáticas/metabolismo , Lactação , Fosfatos/metabolismo , Proteínas na Dieta/administração & dosagem , Radioisótopos de Fósforo/metabolismo , Animais Recém-Nascidos , Animais Lactentes , Peso Corporal , Glucose/administração & dosagem , Ratos Wistar , Fatores de Tempo
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...